Restriction map for
pF1KSDB0040
(Linear) (Six-base) MAPSORT of: KIBB0040.seq Check: 6536 from: 1 to: 1860
KIBB0040.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 647 1267 1287 1541 1860
Size: 647 620 20 254 319
Fragments arranged by size:
647 620 319 254 20
AatII G_ACGT'C
Cuts at: 0 799 1860
Size: 799 1061
AccI GT'mk_AC
Cuts at: 0 539 1860
Size: 539 1321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 543 1860
Size: 412 131 1317
Fragments arranged by size:
1317 412 131
AfeI AGC'GCT
Cuts at: 0 181 1860
Size: 181 1679
AflIII A'CryG_T
Cuts at: 0 482 1860
Size: 482 1378
AhdI GACnn_n'nnGTC
Cuts at: 0 371 724 1860
Size: 371 353 1136
Fragments arranged by size:
1136 371 353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 1860
Size: 34 1826
AlwNI CAG_nnn'CTG
Cuts at: 0 495 1860
Size: 495 1365
AvaI C'yCGr_G
Cuts at: 0 196 1195 1860
Size: 196 999 665
Fragments arranged by size:
999 665 196
AvrII C'CTAG_G
Cuts at: 0 231 1860
Size: 231 1629
BanI G'GyrC_C
Cuts at: 0 337 668 1145 1860
Size: 337 331 477 715
Fragments arranged by size:
715 477 337 331
BanII G_rGCy'C
Cuts at: 0 97 305 335 913 983 1860
Size: 97 208 30 578 70 877
Fragments arranged by size:
877 578 208 97 70 30
BbeI G_GCGC'C
Cuts at: 0 341 1149 1860
Size: 341 808 711
Fragments arranged by size:
808 711 341
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 1053 1419 1860
Size: 405 648 366 441
Fragments arranged by size:
648 441 405 366
BbvCI CC'TCA_GC
Cuts at: 0 424 1860
Size: 424 1436
BglI GCCn_nnn'nGGC
Cuts at: 0 782 1560 1860
Size: 782 778 300
Fragments arranged by size:
782 778 300
BglII A'GATC_T
Cuts at: 0 734 1860
Size: 734 1126
Bme1580I G_kGCm'C
Cuts at: 0 836 1796 1860
Size: 836 960 64
Fragments arranged by size:
960 836 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 319 535 598 613 1069 1102 1465
Size: 310 9 216 63 15 456 33 363
Cuts at: 1465 1764 1860
Size: 299 96
Fragments arranged by size:
456 363 310 299 216 96 63 33
15 9
Bpu10I CC'TnA_GC
Cuts at: 0 424 1860
Size: 424 1436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 706 1860
Size: 490 216 1154
Fragments arranged by size:
1154 490 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 1401 1860
Size: 379 1022 459
Fragments arranged by size:
1022 459 379
BsaAI yAC'GTr
Cuts at: 0 1184 1481 1860
Size: 1184 297 379
Fragments arranged by size:
1184 379 297
BsaBI GATnn'nnATC
Cuts at: 0 466 1860
Size: 466 1394
BsaHI Gr'CG_yC
Cuts at: 0 338 410 796 1146 1709 1860
Size: 338 72 386 350 563 151
Fragments arranged by size:
563 386 350 338 151 72
BsaWI w'CCGG_w
Cuts at: 0 362 1860
Size: 362 1498
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 595 625 1860
Size: 595 30 1235
Fragments arranged by size:
1235 595 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 307 1860
Size: 51 256 1553
Fragments arranged by size:
1553 256 51
BseYI C'CCAG_C
Cuts at: 0 46 90 369 1292 1860
Size: 46 44 279 923 568
Fragments arranged by size:
923 568 279 46 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1345 1860
Size: 625 720 515
Fragments arranged by size:
720 625 515
BsiHKAI G_wGCw'C
Cuts at: 0 145 305 1019 1860
Size: 145 160 714 841
Fragments arranged by size:
841 714 160 145
BsmBI CGTCTCn'nnnn_
Cuts at: 0 208 1860
Size: 208 1652
Bsp1286I G_dGCh'C
Cuts at: 0 97 145 305 335 836 913 983 1019
Size: 97 48 160 30 501 77 70 36
Cuts at: 1019 1796 1860
Size: 777 64
Fragments arranged by size:
777 501 160 97 77 70 64 48
36 30
BspEI T'CCGG_A
Cuts at: 0 362 1860
Size: 362 1498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 647 1016 1267 1287 1541 1860
Size: 647 369 251 20 254 319
Fragments arranged by size:
647 369 319 254 251 20
BsrDI GCAATG_nn'
Cuts at: 0 1416 1860
Size: 1416 444
BsrFI r'CCGG_y
Cuts at: 0 1169 1860
Size: 1169 691
BsrGI T'GTAC_A
Cuts at: 0 497 1860
Size: 497 1363
BstAPI GCAn_nnn'nTGC
Cuts at: 0 927 1860
Size: 927 933
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1686 1860
Size: 1686 174
BstYI r'GATC_y
Cuts at: 0 734 944 1860
Size: 734 210 916
Fragments arranged by size:
916 734 210
Bsu36I CC'TnA_GG
Cuts at: 0 658 1860
Size: 658 1202
BtgI C'CryG_G
Cuts at: 0 116 326 971 993 1685 1860
Size: 116 210 645 22 692 175
Fragments arranged by size:
692 645 210 175 116 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1476 1659 1860
Size: 1476 183 201
Fragments arranged by size:
1476 201 183
BtsI GCAGTG_nn'
Cuts at: 0 813 1860
Size: 813 1047
DraIII CAC_nnn'GTG
Cuts at: 0 1071 1323 1860
Size: 1071 252 537
Fragments arranged by size:
1071 537 252
DrdI GACnn_nn'nnGTC
Cuts at: 0 801 1860
Size: 801 1059
EaeI y'GGCC_r
Cuts at: 0 113 842 968 1496 1860
Size: 113 729 126 528 364
Fragments arranged by size:
729 528 364 126 113
EarI CTCTTCn'nnn_
Cuts at: 0 358 1723 1860
Size: 358 1365 137
Fragments arranged by size:
1365 358 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 135 838 1812 1860
Size: 135 703 974 48
Fragments arranged by size:
974 703 135 48
EcoICRI GAG'CTC
Cuts at: 0 303 1860
Size: 303 1557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 319 412 535 543 598 613 1069
Size: 310 9 93 123 8 55 15 456
Cuts at: 1069 1102 1465 1764 1860
Size: 33 363 299 96
Fragments arranged by size:
456 363 310 299 123 96 93 55
33 15 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 705 1860
Size: 705 1155
EcoO109I rG'GnC_Cy
Cuts at: 0 492 1860
Size: 492 1368
FspI TGC'GCA
Cuts at: 0 1543 1860
Size: 1543 317
HaeII r_GCGC'y
Cuts at: 0 183 326 341 631 1149 1860
Size: 183 143 15 290 518 711
Fragments arranged by size:
711 518 290 183 143 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 357 389 616 648 710 742 1860
Size: 357 32 227 32 62 32 1118
Fragments arranged by size:
1118 357 227 62 32 32 32
KasI G'GCGC_C
Cuts at: 0 337 1145 1860
Size: 337 808 715
Fragments arranged by size:
808 715 337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1622 1784 1860
Size: 1622 162 76
Fragments arranged by size:
1622 162 76
MslI CAynn'nnrTG
Cuts at: 0 1535 1684 1852 1860
Size: 1535 149 168 8
Fragments arranged by size:
1535 168 149 8
MspA1I CmG'CkG
Cuts at: 0 134 563 585 829 951 966 995 1038
Size: 134 429 22 244 122 15 29 43
Cuts at: 1038 1110 1151 1860
Size: 72 41 709
Fragments arranged by size:
709 429 244 134 122 72 43 41
29 22 15
NarI GG'CG_CC
Cuts at: 0 338 1146 1860
Size: 338 808 714
Fragments arranged by size:
808 714 338
NcoI C'CATG_G
Cuts at: 0 1685 1860
Size: 1685 175
NspI r_CATG'y
Cuts at: 0 45 486 1540 1860
Size: 45 441 1054 320
Fragments arranged by size:
1054 441 320 45
PciI A'CATG_T
Cuts at: 0 482 1860
Size: 482 1378
PflMI CCAn_nnn'nTGG
Cuts at: 0 954 1071 1370 1860
Size: 954 117 299 490
Fragments arranged by size:
954 490 299 117
PfoI T'CCnGG_A
Cuts at: 0 689 1094 1277 1860
Size: 689 405 183 583
Fragments arranged by size:
689 583 405 183
PmlI CAC'GTG
Cuts at: 0 1184 1860
Size: 1184 676
PpuMI rG'GwC_Cy
Cuts at: 0 492 1860
Size: 492 1368
PstI C_TGCA'G
Cuts at: 0 941 1860
Size: 941 919
PvuII CAG'CTG
Cuts at: 0 585 951 966 1038 1860
Size: 585 366 15 72 822
Fragments arranged by size:
822 585 366 72 15
SacI G_AGCT'C
Cuts at: 0 305 1860
Size: 305 1555
SacII CC_GC'GG
Cuts at: 0 996 1860
Size: 996 864
SexAI A'CCwGG_T
Cuts at: 0 1391 1860
Size: 1391 469
SfcI C'TryA_G
Cuts at: 0 937 1860
Size: 937 923
SfoI GGC'GCC
Cuts at: 0 339 1147 1860
Size: 339 808 713
Fragments arranged by size:
808 713 339
SmlI C'TyrA_G
Cuts at: 0 505 721 1860
Size: 505 216 1139
Fragments arranged by size:
1139 505 216
SphI G_CATG'C
Cuts at: 0 45 1860
Size: 45 1815
StyI C'CwwG_G
Cuts at: 0 231 1685 1860
Size: 231 1454 175
Fragments arranged by size:
1454 231 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 783 1125 1860
Size: 783 342 735
Fragments arranged by size:
783 735 342
TatI w'GTAC_w
Cuts at: 0 497 1860
Size: 497 1363
Tth111I GACn'n_nGTC
Cuts at: 0 535 1860
Size: 535 1325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 81 1860
Size: 54 27 1779
Fragments arranged by size:
1779 54 27
ZraI GAC'GTC
Cuts at: 0 797 1860
Size: 797 1063
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlfI
AlwNI AvaI AvrII BanI BanII BbeI BbsI BbvCI
BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MmeI MslI MspA1I NarI NcoI
NspI PciI PflMI PfoI PmlI PpuMI PstI PvuII
SacI SacII SexAI SfcI SfoI SmlI SphI StyI
TaqII TatI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AloI ApaI ApaLI
ApoI AscI AseI AsiSI BaeI BamHI BcgI BciVI
BclI BfrBI BlpI BmgBI BmrI BmtI BplI BsiEI
BsiWI BsmI BspHI BsrBI BssHII BssSI BstBI BstEII
BstZ17I ClaI CspCI DraI EagI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI XbaI XhoI XmaI XmnI