Restriction map for
pF1KSDB0036
(Linear) (Six-base) MAPSORT of: KIBB0036.seq Check: 8187 from: 1 to: 1935
KIBB0036.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 361 1935
Size: 361 1574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1406 1935
Size: 1319 87 529
Fragments arranged by size:
1319 529 87
AflII C'TTAA_G
Cuts at: 0 315 1935
Size: 315 1620
AflIII A'CryG_T
Cuts at: 0 302 356 1935
Size: 302 54 1579
Fragments arranged by size:
1579 302 54
AleI CACnn'nnGTG
Cuts at: 0 439 1935
Size: 439 1496
AlwNI CAG_nnn'CTG
Cuts at: 0 15 1935
Size: 15 1920
ApaI G_GGCC'C
Cuts at: 0 110 1935
Size: 110 1825
ApaLI G'TGCA_C
Cuts at: 0 141 1935
Size: 141 1794
ApoI r'AATT_y
Cuts at: 0 1814 1935
Size: 1814 121
AseI AT'TA_AT
Cuts at: 0 647 1935
Size: 647 1288
BanI G'GyrC_C
Cuts at: 0 1758 1935
Size: 1758 177
BanII G_rGCy'C
Cuts at: 0 110 1935
Size: 110 1825
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 811 911 1935
Size: 216 595 100 1024
Fragments arranged by size:
1024 595 216 100
BbvCI CC'TCA_GC
Cuts at: 0 1089 1935
Size: 1089 846
BciVI GTATCCnnnnn_n'
Cuts at: 0 75 1092 1935
Size: 75 1017 843
Fragments arranged by size:
1017 843 75
Bme1580I G_kGCm'C
Cuts at: 0 110 145 1935
Size: 110 35 1790
Fragments arranged by size:
1790 110 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 1706 1935
Size: 1706 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 1935
Size: 1385 550
Bpu10I CC'TnA_GC
Cuts at: 0 1089 1935
Size: 1089 846
BsaI GGTCTCn'nnnn_
Cuts at: 0 1878 1935
Size: 1878 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1247 1277 1383 1413 1935
Size: 1247 30 106 30 522
Fragments arranged by size:
1247 522 106 30 30
BseYI C'CCAG_C
Cuts at: 0 38 1935
Size: 38 1897
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1935
Size: 1094 841
BsiHKAI G_wGCw'C
Cuts at: 0 145 1920 1935
Size: 145 1775 15
Fragments arranged by size:
1775 145 15
BsmI GAATG_Cn'
Cuts at: 0 1749 1935
Size: 1749 186
Bsp1286I G_dGCh'C
Cuts at: 0 110 145 1920 1935
Size: 110 35 1775 15
Fragments arranged by size:
1775 110 35 15
BspHI T'CATG_A
Cuts at: 0 1371 1880 1935
Size: 1371 509 55
Fragments arranged by size:
1371 509 55
BsrDI GCAATG_nn'
Cuts at: 0 561 1935
Size: 561 1374
BsrFI r'CCGG_y
Cuts at: 0 52 1935
Size: 52 1883
BsrGI T'GTAC_A
Cuts at: 0 1212 1935
Size: 1212 723
BssSI C'ACGA_G
Cuts at: 0 94 1935
Size: 94 1841
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1620 1935
Size: 1620 315
BstYI r'GATC_y
Cuts at: 0 1702 1935
Size: 1702 233
BtsI GCAGTG_nn'
Cuts at: 0 29 920 1935
Size: 29 891 1015
Fragments arranged by size:
1015 891 29
DraI TTT'AAA
Cuts at: 0 392 1013 1935
Size: 392 621 922
Fragments arranged by size:
922 621 392
EaeI y'GGCC_r
Cuts at: 0 6 1935
Size: 6 1929
EarI CTCTTCn'nnn_
Cuts at: 0 439 1935
Size: 439 1496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1385 1406 1935
Size: 1319 66 21 529
Fragments arranged by size:
1319 529 66 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 802 1910 1935
Size: 802 1108 25
Fragments arranged by size:
1108 802 25
EcoO109I rG'GnC_Cy
Cuts at: 0 106 807 1935
Size: 106 701 1128
Fragments arranged by size:
1128 701 106
EcoRI G'AATT_C
Cuts at: 0 1814 1935
Size: 1814 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 715 1935
Size: 715 1220
HaeII r_GCGC'y
Cuts at: 0 1026 1935
Size: 1026 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1353 1385 1432 1464 1935
Size: 1353 32 47 32 471
Fragments arranged by size:
1353 471 47 32 32
HincII GTy'rAC
Cuts at: 0 1226 1440 1675 1694 1935
Size: 1226 214 235 19 241
Fragments arranged by size:
1226 241 235 214 19
HindIII A'AGCT_T
Cuts at: 0 1007 1281 1935
Size: 1007 274 654
Fragments arranged by size:
1007 654 274
HpaI GTT'AAC
Cuts at: 0 1694 1935
Size: 1694 241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 750 1365 1935
Size: 635 115 615 570
Fragments arranged by size:
635 615 570 115
MslI CAynn'nnrTG
Cuts at: 0 439 1935
Size: 439 1496
MspA1I CmG'CkG
Cuts at: 0 883 1426 1935
Size: 883 543 509
Fragments arranged by size:
883 543 509
NspI r_CATG'y
Cuts at: 0 360 1935
Size: 360 1575
PciI A'CATG_T
Cuts at: 0 356 1935
Size: 356 1579
PspOMI G'GGCC_C
Cuts at: 0 106 1935
Size: 106 1829
PstI C_TGCA'G
Cuts at: 0 1071 1935
Size: 1071 864
PvuII CAG'CTG
Cuts at: 0 883 1426 1935
Size: 883 543 509
Fragments arranged by size:
883 543 509
RsrII CG'GwC_CG
Cuts at: 0 21 1935
Size: 21 1914
SexAI A'CCwGG_T
Cuts at: 0 1677 1935
Size: 1677 258
SfcI C'TryA_G
Cuts at: 0 878 1067 1935
Size: 878 189 868
Fragments arranged by size:
878 868 189
SmlI C'TyrA_G
Cuts at: 0 315 1935
Size: 315 1620
SspI AAT'ATT
Cuts at: 0 664 1935
Size: 664 1271
StyI C'CwwG_G
Cuts at: 0 851 1935
Size: 851 1084
SwaI ATTT'AAAT
Cuts at: 0 392 1935
Size: 392 1543
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 38 204 1220 1935
Size: 38 166 1016 715
Fragments arranged by size:
1016 715 166 38
TatI w'GTAC_w
Cuts at: 0 1212 1246 1935
Size: 1212 34 689
Fragments arranged by size:
1212 689 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1383 1415 1935
Size: 1383 32 520
Fragments arranged by size:
1383 520 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1736 1935
Size: 1736 199
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaI ApaLI
ApoI AseI BanI BanII BbsI BbvCI BciVI Bme1580I
BmrI BpmI Bpu10I BsaI BsaXI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstXI
BstYI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII HpaI MmeI
MslI MspA1I NspI PciI PspOMI PstI PvuII RsrII
SexAI SfcI SmlI SspI StyI SwaI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI AscI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI Tth111I XbaI XhoI XmaI
XmnI ZraI