Restriction map for
pF1KSDB0033
(Linear) (Six-base) MAPSORT of: KIBB0033.seq Check: 8481 from: 1 to: 3189
KIBB0033.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2520 3189
Size: 2520 669
Acc65I G'GTAC_C
Cuts at: 0 21 3189
Size: 21 3168
AflII C'TTAA_G
Cuts at: 0 1708 3189
Size: 1708 1481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 485 495 553 3189
Size: 485 10 58 2636
Fragments arranged by size:
2636 485 58 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 3189
Size: 565 32 2592
Fragments arranged by size:
2592 565 32
AlwNI CAG_nnn'CTG
Cuts at: 0 65 729 2208 2610 2820 3189
Size: 65 664 1479 402 210 369
Fragments arranged by size:
1479 664 402 369 210 65
ApaI G_GGCC'C
Cuts at: 0 3085 3189
Size: 3085 104
ApaLI G'TGCA_C
Cuts at: 0 682 3189
Size: 682 2507
ApoI r'AATT_y
Cuts at: 0 1621 2257 3180 3189
Size: 1621 636 923 9
Fragments arranged by size:
1621 923 636 9
AvaI C'yCGr_G
Cuts at: 0 490 1249 1501 2407 2723 3189
Size: 490 759 252 906 316 466
Fragments arranged by size:
906 759 490 466 316 252
BanI G'GyrC_C
Cuts at: 0 21 502 628 2766 3097 3152 3189
Size: 21 481 126 2138 331 55 37
Fragments arranged by size:
2138 481 331 126 55 37 21
BanII G_rGCy'C
Cuts at: 0 1094 1102 1225 1256 1355 1409 1772 1859
Size: 1094 8 123 31 99 54 363 87
Cuts at: 1859 2746 2753 3085 3189
Size: 887 7 332 104
Fragments arranged by size:
1094 887 363 332 123 104 99 87
54 31 8 7
BbvCI CC'TCA_GC
Cuts at: 0 1024 3189
Size: 1024 2165
BciVI GTATCCnnnnn_n'
Cuts at: 0 1536 3189
Size: 1536 1653
BglII A'GATC_T
Cuts at: 0 281 2141 2636 3189
Size: 281 1860 495 553
Fragments arranged by size:
1860 553 495 281
BlpI GC'TnA_GC
Cuts at: 0 913 3189
Size: 913 2276
Bme1580I G_kGCm'C
Cuts at: 0 338 633 686 2038 3085 3155 3189
Size: 338 295 53 1352 1047 70 34
Fragments arranged by size:
1352 1047 338 295 70 53 34
BmgBI CAC'GTC
Cuts at: 0 1215 2045 3189
Size: 1215 830 1144
Fragments arranged by size:
1215 1144 830
BmrI ACTGGGnnnn_n'
Cuts at: 0 1800 2323 3189
Size: 1800 523 866
Fragments arranged by size:
1800 866 523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 3189
Size: 1156 2033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 562 567 751 1132 1549 1558 2209
Size: 181 381 5 184 381 417 9 651
Cuts at: 2209 2253 3189
Size: 44 936
Fragments arranged by size:
936 651 417 381 381 184 181 44
9 5
Bpu10I CC'TnA_GC
Cuts at: 0 1024 1096 2914 3189
Size: 1024 72 1818 275
Fragments arranged by size:
1818 1024 275 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1345 3189
Size: 988 357 1844
Fragments arranged by size:
1844 988 357
BsaI GGTCTCn'nnnn_
Cuts at: 0 258 469 1708 3189
Size: 258 211 1239 1481
Fragments arranged by size:
1481 1239 258 211
BsaAI yAC'GTr
Cuts at: 0 2054 2994 3189
Size: 2054 940 195
Fragments arranged by size:
2054 940 195
BsaBI GATnn'nnATC
Cuts at: 0 2146 3189
Size: 2146 1043
BsaHI Gr'CG_yC
Cuts at: 0 317 1136 2855 3189
Size: 317 819 1719 334
Fragments arranged by size:
1719 819 334 317
BsaWI w'CCGG_w
Cuts at: 0 569 1700 1760 2893 3189
Size: 569 1131 60 1133 296
Fragments arranged by size:
1133 1131 569 296 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 491 521 565 595 1441 1471 1705 1735
Size: 491 30 44 30 846 30 234 30
Cuts at: 1735 2960 2990 3189
Size: 1225 30 199
Fragments arranged by size:
1225 846 491 234 199 44 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 760 816 982 1012 1102 1390 1465
Size: 757 3 56 166 30 90 288 75
Cuts at: 1465 1501 3189
Size: 36 1688
Fragments arranged by size:
1688 757 288 166 90 75 56 36
30 3
BseYI C'CCAG_C
Cuts at: 0 483 762 1225 3189
Size: 483 279 463 1964
Fragments arranged by size:
1964 483 463 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 631 1618 3189
Size: 163 468 987 1571
Fragments arranged by size:
1571 987 468 163
BsiEI CG_ry'CG
Cuts at: 0 1799 1905 2068 2787 3189
Size: 1799 106 163 719 402
Fragments arranged by size:
1799 719 402 163 106
BsiHKAI G_wGCw'C
Cuts at: 0 378 686 1094 1240 1355 1772 3189
Size: 378 308 408 146 115 417 1417
Fragments arranged by size:
1417 417 408 378 308 146 115
BsiWI C'GTAC_G
Cuts at: 0 1124 3189
Size: 1124 2065
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1127 1786 2035 2995 3189
Size: 1127 659 249 960 194
Fragments arranged by size:
1127 960 659 249 194
Bsp1286I G_dGCh'C
Cuts at: 0 338 378 633 686 1094 1102 1225 1240
Size: 338 40 255 53 408 8 123 15
Cuts at: 1240 1256 1355 1409 1772 1859 2038 2746 2753
Size: 16 99 54 363 87 179 708 7
Cuts at: 2753 3085 3155 3189
Size: 332 70 34
Fragments arranged by size:
708 408 363 338 332 255 179 123
99 87 70 54 53 40 34 16
15 8 7
BspEI T'CCGG_A
Cuts at: 0 569 1700 3189
Size: 569 1131 1489
Fragments arranged by size:
1489 1131 569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 284 487 578 917 1970 2520 3189
Size: 206 78 203 91 339 1053 550 669
Fragments arranged by size:
1053 669 550 339 206 203 91 78
BsrBI CCG'CTC
Cuts at: 0 425 702 3189
Size: 425 277 2487
Fragments arranged by size:
2487 425 277
BsrDI GCAATG_nn'
Cuts at: 0 981 3189
Size: 981 2208
BsrFI r'CCGG_y
Cuts at: 0 290 515 1978 3189
Size: 290 225 1463 1211
Fragments arranged by size:
1463 1211 290 225
BsrGI T'GTAC_A
Cuts at: 0 1154 2940 3189
Size: 1154 1786 249
Fragments arranged by size:
1786 1154 249
BssSI C'ACGA_G
Cuts at: 0 674 1130 2493 3189
Size: 674 456 1363 696
Fragments arranged by size:
1363 696 674 456
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2610 2820 3189
Size: 2610 210 369
Fragments arranged by size:
2610 369 210
BstEII G'GTnAC_C
Cuts at: 0 99 984 1650 3189
Size: 99 885 666 1539
Fragments arranged by size:
1539 885 666 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 694 1215 2045 3189
Size: 694 521 830 1144
Fragments arranged by size:
1144 830 694 521
BstYI r'GATC_y
Cuts at: 0 281 1838 2141 2219 2636 3189
Size: 281 1557 303 78 417 553
Fragments arranged by size:
1557 553 417 303 281 78
Bsu36I CC'TnA_GG
Cuts at: 0 2458 3189
Size: 2458 731
BtgI C'CryG_G
Cuts at: 0 104 312 353 635 1241 2624 3172 3189
Size: 104 208 41 282 606 1383 548 17
Fragments arranged by size:
1383 606 548 282 208 104 41 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1059 1380 3189
Size: 1059 321 1809
Fragments arranged by size:
1809 1059 321
BtsI GCAGTG_nn'
Cuts at: 0 90 376 3189
Size: 90 286 2813
Fragments arranged by size:
2813 286 90
DraIII CAC_nnn'GTG
Cuts at: 0 1935 2495 3189
Size: 1935 560 694
Fragments arranged by size:
1935 694 560
DrdI GACnn_nn'nnGTC
Cuts at: 0 853 2860 3189
Size: 853 2007 329
Fragments arranged by size:
2007 853 329
EaeI y'GGCC_r
Cuts at: 0 642 923 1786 1902 2065 2210 3189
Size: 642 281 863 116 163 145 979
Fragments arranged by size:
979 863 642 281 163 145 116
EagI C'GGCC_G
Cuts at: 0 1902 2065 3189
Size: 1902 163 1124
Fragments arranged by size:
1902 1124 163
EarI CTCTTCn'nnn_
Cuts at: 0 758 1348 1702 2111 2266 2689 3189
Size: 758 590 354 409 155 423 500
Fragments arranged by size:
758 590 500 423 409 354 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 373 1248 1615 1915 3189
Size: 373 875 367 300 1274
Fragments arranged by size:
1274 875 373 367 300
EcoICRI GAG'CTC
Cuts at: 0 1092 1353 1770 3189
Size: 1092 261 417 1419
Fragments arranged by size:
1419 1092 417 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 562 567 751 1132 1549 1558 2209
Size: 181 381 5 184 381 417 9 651
Cuts at: 2209 2253 3189
Size: 44 936
Fragments arranged by size:
936 651 417 381 381 184 181 44
9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 802 1114 1144 2521 3189
Size: 802 312 30 1377 668
Fragments arranged by size:
1377 802 668 312 30
EcoO109I rG'GnC_Cy
Cuts at: 0 62 273 906 3081 3082 3189
Size: 62 211 633 2175 1 107
Fragments arranged by size:
2175 633 211 107 62 1
EcoRI G'AATT_C
Cuts at: 0 1621 3180 3189
Size: 1621 1559 9
Fragments arranged by size:
1621 1559 9
FspI TGC'GCA
Cuts at: 0 382 1972 2437 3189
Size: 382 1590 465 752
Fragments arranged by size:
1590 752 465 382
FspAI rTGC'GCAy
Cuts at: 0 2437 3189
Size: 2437 752
HaeII r_GCGC'y
Cuts at: 0 7 3189
Size: 7 3182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2267 2299 2366 2398 2907 2939 3189
Size: 2267 32 67 32 509 32 250
Fragments arranged by size:
2267 509 250 67 32 32 32
HindIII A'AGCT_T
Cuts at: 0 70 2006 3189
Size: 70 1936 1183
Fragments arranged by size:
1936 1183 70
KpnI G_GTAC'C
Cuts at: 0 25 3189
Size: 25 3164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1346 1934 2118 3189
Size: 1346 588 184 1071
Fragments arranged by size:
1346 1071 588 184
MscI TGG'CCA
Cuts at: 0 644 925 2212 3189
Size: 644 281 1287 977
Fragments arranged by size:
1287 977 644 281
MslI CAynn'nnrTG
Cuts at: 0 43 607 3189
Size: 43 564 2582
Fragments arranged by size:
2582 564 43
MspA1I CmG'CkG
Cuts at: 0 726 1104 1229 1593 1830 1871 2402 2469
Size: 726 378 125 364 237 41 531 67
Cuts at: 2469 2610 2817 3189
Size: 141 207 372
Fragments arranged by size:
726 531 378 372 364 237 207 141
125 67 41
NaeI GCC'GGC
Cuts at: 0 292 1980 3189
Size: 292 1688 1209
Fragments arranged by size:
1688 1209 292
NcoI C'CATG_G
Cuts at: 0 104 3189
Size: 104 3085
NdeI CA'TA_TG
Cuts at: 0 299 3189
Size: 299 2890
NgoMIV G'CCGG_C
Cuts at: 0 290 1978 3189
Size: 290 1688 1211
Fragments arranged by size:
1688 1211 290
PasI CC'CwG_GG
Cuts at: 0 78 3189
Size: 78 3111
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 3189
Size: 729 2460
PfoI T'CCnGG_A
Cuts at: 0 662 1256 1526 2423 2483 3189
Size: 662 594 270 897 60 706
Fragments arranged by size:
897 706 662 594 270 60
PmlI CAC'GTG
Cuts at: 0 2054 2994 3189
Size: 2054 940 195
Fragments arranged by size:
2054 940 195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 3189
Size: 565 32 2592
Fragments arranged by size:
2592 565 32
PpuMI rG'GwC_Cy
Cuts at: 0 273 906 3189
Size: 273 633 2283
Fragments arranged by size:
2283 633 273
PspOMI G'GGCC_C
Cuts at: 0 3081 3189
Size: 3081 108
PstI C_TGCA'G
Cuts at: 0 281 464 1337 1829 1847 2615 2735 2984
Size: 281 183 873 492 18 768 120 249
Cuts at: 2984 3189
Size: 205
Fragments arranged by size:
873 768 492 281 249 205 183 120
18
PvuII CAG'CTG
Cuts at: 0 726 1229 1830 2469 2610 2817 3189
Size: 726 503 601 639 141 207 372
Fragments arranged by size:
726 639 601 503 372 207 141
SacI G_AGCT'C
Cuts at: 0 1094 1355 1772 3189
Size: 1094 261 417 1417
Fragments arranged by size:
1417 1094 417 261
SapI GCTCTTCn'nnn_
Cuts at: 0 1348 2689 3189
Size: 1348 1341 500
Fragments arranged by size:
1348 1341 500
SexAI A'CCwGG_T
Cuts at: 0 797 1931 3189
Size: 797 1134 1258
Fragments arranged by size:
1258 1134 797
SfcI C'TryA_G
Cuts at: 0 277 285 460 523 1333 1656 1825 1843
Size: 277 8 175 63 810 323 169 18
Cuts at: 1843 2611 2731 2980 3189
Size: 768 120 249 209
Fragments arranged by size:
810 768 323 277 249 209 175 169
120 63 18 8
SmaI CCC'GGG
Cuts at: 0 1503 3189
Size: 1503 1686
SmlI C'TyrA_G
Cuts at: 0 1003 1360 1708 3189
Size: 1003 357 348 1481
Fragments arranged by size:
1481 1003 357 348
StuI AGG'CCT
Cuts at: 0 547 2527 2737 3189
Size: 547 1980 210 452
Fragments arranged by size:
1980 547 452 210
StyI C'CwwG_G
Cuts at: 0 104 1079 1145 1208 2738 3137 3189
Size: 104 975 66 63 1530 399 52
Fragments arranged by size:
1530 975 399 104 66 63 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 628 1819 3189
Size: 454 174 1191 1370
Fragments arranged by size:
1370 1191 454 174
TatI w'GTAC_w
Cuts at: 0 599 1154 2120 2940 3189
Size: 599 555 966 820 249
Fragments arranged by size:
966 820 599 555 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 1474 1506 1705 1737 2342 2374
Size: 491 32 951 32 199 32 605 32
Cuts at: 2374 2479 2511 2960 2992 3189
Size: 105 32 449 32 197
Fragments arranged by size:
951 605 491 449 199 197 105 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1605 3189
Size: 1605 1584
XmaI C'CCGG_G
Cuts at: 0 1501 3189
Size: 1501 1688
XmnI GAAnn'nnTTC
Cuts at: 0 1280 2416 3189
Size: 1280 1136 773
Fragments arranged by size:
1280 1136 773
Enzymes that do cut:
AarI Acc65I AflII AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbvCI BciVI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HindIII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI
SexAI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflIII AgeI AhdI
AleI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BclI BfrBI BglI BmtI BsmI BspHI
BssHII BstBI BstZ17I ClaI CspCI DraI EcoRV FalI
FseI HincII HpaI KasI MfeI MluI NarI NheI
NotI NruI NsiI NspI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI