Restriction map for
pF1KSDB0032
(Linear) (Six-base) MAPSORT of: KIBB0032.seq Check: 4464 from: 1 to: 1437
KIBB0032.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 921 1437
Size: 921 516
AatII G_ACGT'C
Cuts at: 0 915 1437
Size: 915 522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1030 1242 1355 1437
Size: 574 456 212 113 82
Fragments arranged by size:
574 456 212 113 82
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 113 1437
Size: 113 1324
ApoI r'AATT_y
Cuts at: 0 370 845 919 1136 1437
Size: 370 475 74 217 301
Fragments arranged by size:
475 370 301 217 74
AvaI C'yCGr_G
Cuts at: 0 626 1437
Size: 626 811
BbsI GAAGACnn'nnnn_
Cuts at: 0 1069 1437
Size: 1069 368
BglII A'GATC_T
Cuts at: 0 335 463 1437
Size: 335 128 974
Fragments arranged by size:
974 335 128
BlpI GC'TnA_GC
Cuts at: 0 86 1437
Size: 86 1351
BmrI ACTGGGnnnn_n'
Cuts at: 0 319 1437
Size: 319 1118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1364 1437
Size: 1364 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1437
Size: 1078 359
BsaBI GATnn'nnATC
Cuts at: 0 1160 1437
Size: 1160 277
BsaHI Gr'CG_yC
Cuts at: 0 912 1437
Size: 912 525
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 458 1437
Size: 458 979
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1437
Size: 49 1388
BsmI GAATG_Cn'
Cuts at: 0 908 1437
Size: 908 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 921 1437
Size: 921 516
BsrDI GCAATG_nn'
Cuts at: 0 318 425 951 1013 1437
Size: 318 107 526 62 424
Fragments arranged by size:
526 424 318 107 62
BsrGI T'GTAC_A
Cuts at: 0 302 1437
Size: 302 1135
BssHII G'CGCG_C
Cuts at: 0 690 1437
Size: 690 747
BstYI r'GATC_y
Cuts at: 0 252 335 463 1437
Size: 252 83 128 974
Fragments arranged by size:
974 252 128 83
BtsI GCAGTG_nn'
Cuts at: 0 436 1437
Size: 436 1001
DraI TTT'AAA
Cuts at: 0 768 1349 1437
Size: 768 581 88
Fragments arranged by size:
768 581 88
EaeI y'GGCC_r
Cuts at: 0 810 1437
Size: 810 627
EarI CTCTTCn'nnn_
Cuts at: 0 740 1437
Size: 740 697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1030 1242 1355 1364 1437
Size: 574 456 212 113 9 73
Fragments arranged by size:
574 456 212 113 73 9
EcoO109I rG'GnC_Cy
Cuts at: 0 967 1437
Size: 967 470
EcoRI G'AATT_C
Cuts at: 0 1136 1437
Size: 1136 301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1156 1437
Size: 1156 281
HincII GTy'rAC
Cuts at: 0 165 1437
Size: 165 1272
HindIII A'AGCT_T
Cuts at: 0 107 615 1437
Size: 107 508 822
Fragments arranged by size:
822 508 107
HpaI GTT'AAC
Cuts at: 0 165 1437
Size: 165 1272
MfeI C'AATT_G
Cuts at: 0 1292 1437
Size: 1292 145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1437
Size: 214 1223
MscI TGG'CCA
Cuts at: 0 812 1437
Size: 812 625
MspA1I CmG'CkG
Cuts at: 0 33 1333 1437
Size: 33 1300 104
Fragments arranged by size:
1300 104 33
NdeI CA'TA_TG
Cuts at: 0 608 1437
Size: 608 829
NspI r_CATG'y
Cuts at: 0 303 1437
Size: 303 1134
PflMI CCAn_nnn'nTGG
Cuts at: 0 808 1099 1437
Size: 808 291 338
Fragments arranged by size:
808 338 291
PpuMI rG'GwC_Cy
Cuts at: 0 967 1437
Size: 967 470
PshAI GACnn'nnGTC
Cuts at: 0 703 1437
Size: 703 734
PsiI TTA'TAA
Cuts at: 0 1028 1437
Size: 1028 409
PspXI vC'TCGA_Gb
Cuts at: 0 626 1437
Size: 626 811
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1311 1343 1437
Size: 1311 32 94
Fragments arranged by size:
1311 94 32
PstI C_TGCA'G
Cuts at: 0 443 654 1002 1437
Size: 443 211 348 435
Fragments arranged by size:
443 435 348 211
PvuII CAG'CTG
Cuts at: 0 33 1333 1437
Size: 33 1300 104
Fragments arranged by size:
1300 104 33
SfcI C'TryA_G
Cuts at: 0 240 356 439 650 956 998 1328 1437
Size: 240 116 83 211 306 42 330 109
Fragments arranged by size:
330 306 240 211 116 109 83 42
SmlI C'TyrA_G
Cuts at: 0 626 1057 1437
Size: 626 431 380
Fragments arranged by size:
626 431 380
SspI AAT'ATT
Cuts at: 0 1408 1437
Size: 1408 29
StyI C'CwwG_G
Cuts at: 0 813 1437
Size: 813 624
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 1239 1437
Size: 165 1074 198
Fragments arranged by size:
1074 198 165
TatI w'GTAC_w
Cuts at: 0 302 1383 1437
Size: 302 1081 54
Fragments arranged by size:
1081 302 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 479 511 1437
Size: 479 32 926
Fragments arranged by size:
926 479 32
XhoI C'TCGA_G
Cuts at: 0 626 1437
Size: 626 811
XmnI GAAnn'nnTTC
Cuts at: 0 452 1323 1437
Size: 452 871 114
Fragments arranged by size:
871 452 114
ZraI GAC'GTC
Cuts at: 0 913 1437
Size: 913 524
Enzymes that do cut:
AarI AatII AcuI AlfI ApoI AvaI BbsI BglII
BlpI BmrI BpmI BpuEI BsaBI BsaHI BseRI BsgI
BsmI BspMI BsrDI BsrGI BssHII BstYI BtsI DraI
EaeI EarI Eco57MI EcoO109I EcoRI FalI HincII HindIII
HpaI MfeI MmeI MscI MspA1I NdeI NspI PflMI
PpuMI PshAI PsiI PspXI PsrI PstI PvuII SfcI
SmlI SspI StyI TaqII TatI TstI XhoI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HaeII Hin4I KasI KpnI
MluI MslI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PspOMI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XbaI XcmI XmaI