Restriction map for
pF1KSDB0029
(Linear) (Six-base) MAPSORT of: KIBB0029.seq Check: 9654 from: 1 to: 1710
KIBB0029.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 283 565 1710
Size: 283 282 1145
Fragments arranged by size:
1145 283 282
AccI GT'mk_AC
Cuts at: 0 1433 1710
Size: 1433 277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 1710
Size: 990 720
AleI CACnn'nnGTG
Cuts at: 0 1630 1710
Size: 1630 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1372 1710
Size: 1372 338
AlwNI CAG_nnn'CTG
Cuts at: 0 1246 1552 1710
Size: 1246 306 158
Fragments arranged by size:
1246 306 158
AvaI C'yCGr_G
Cuts at: 0 1556 1710
Size: 1556 154
BamHI G'GATC_C
Cuts at: 0 1236 1710
Size: 1236 474
BanI G'GyrC_C
Cuts at: 0 298 324 1060 1537 1624 1671 1710
Size: 298 26 736 477 87 47 39
Fragments arranged by size:
736 477 298 87 47 39 26
BanII G_rGCy'C
Cuts at: 0 542 833 925 1326 1564 1710
Size: 542 291 92 401 238 146
Fragments arranged by size:
542 401 291 238 146 92
BbeI G_GCGC'C
Cuts at: 0 1675 1710
Size: 1675 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 172 1710
Size: 172 1538
BbvCI CC'TCA_GC
Cuts at: 0 698 1710
Size: 698 1012
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1647 1681 1710
Size: 1647 34 29
Fragments arranged by size:
1647 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 1193 1710
Size: 1193 517
BclI T'GATC_A
Cuts at: 0 81 308 1710
Size: 81 227 1402
Fragments arranged by size:
1402 227 81
BglI GCCn_nnn'nGGC
Cuts at: 0 662 892 1622 1710
Size: 662 230 730 88
Fragments arranged by size:
730 662 230 88
BglII A'GATC_T
Cuts at: 0 548 1169 1710
Size: 548 621 541
Fragments arranged by size:
621 548 541
BlpI GC'TnA_GC
Cuts at: 0 1650 1710
Size: 1650 60
Bme1580I G_kGCm'C
Cuts at: 0 996 1063 1710
Size: 996 67 647
Fragments arranged by size:
996 647 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 891 1710
Size: 891 819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 221 1710
Size: 176 45 1489
Fragments arranged by size:
1489 176 45
Bpu10I CC'TnA_GC
Cuts at: 0 288 329 698 919 1710
Size: 288 41 369 221 791
Fragments arranged by size:
791 369 288 221 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1710
Size: 569 1141
BsaAI yAC'GTr
Cuts at: 0 1380 1710
Size: 1380 330
BsaBI GATnn'nnATC
Cuts at: 0 193 518 1710
Size: 193 325 1192
Fragments arranged by size:
1192 325 193
BsaHI Gr'CG_yC
Cuts at: 0 1672 1710
Size: 1672 38
BsaWI w'CCGG_w
Cuts at: 0 939 1710
Size: 939 771
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 937 967 1069 1099 1710
Size: 937 30 102 30 611
Fragments arranged by size:
937 611 102 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 173 1090 1316 1710
Size: 173 917 226 394
Fragments arranged by size:
917 394 226 173
BseYI C'CCAG_C
Cuts at: 0 570 1710
Size: 570 1140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 797 984 1360 1710
Size: 475 322 187 376 350
Fragments arranged by size:
475 376 350 322 187
BsiEI CG_ry'CG
Cuts at: 0 1576 1686 1710
Size: 1576 110 24
Fragments arranged by size:
1576 110 24
BsiHKAI G_wGCw'C
Cuts at: 0 542 1710
Size: 542 1168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 52 1263 1710
Size: 52 1211 447
Fragments arranged by size:
1211 447 52
Bsp1286I G_dGCh'C
Cuts at: 0 542 833 925 996 1063 1326 1564 1710
Size: 542 291 92 71 67 263 238 146
Fragments arranged by size:
542 291 263 238 146 92 71 67
BspEI T'CCGG_A
Cuts at: 0 939 1710
Size: 939 771
BspHI T'CATG_A
Cuts at: 0 764 1710
Size: 764 946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 283 565 1382 1478 1710
Size: 283 282 817 96 232
Fragments arranged by size:
817 283 282 232 96
BsrBI CCG'CTC
Cuts at: 0 1080 1710
Size: 1080 630
BsrDI GCAATG_nn'
Cuts at: 0 578 611 722 1710
Size: 578 33 111 988
Fragments arranged by size:
988 578 111 33
BssSI C'ACGA_G
Cuts at: 0 36 1710
Size: 36 1674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 453 1552 1710
Size: 453 1099 158
Fragments arranged by size:
1099 453 158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1071 1393 1710
Size: 1071 322 317
Fragments arranged by size:
1071 322 317
BstYI r'GATC_y
Cuts at: 0 548 972 1169 1236 1710
Size: 548 424 197 67 474
Fragments arranged by size:
548 474 424 197 67
Bsu36I CC'TnA_GG
Cuts at: 0 1055 1710
Size: 1055 655
BtgI C'CryG_G
Cuts at: 0 28 1710
Size: 28 1682
BtsI GCAGTG_nn'
Cuts at: 0 836 996 1010 1710
Size: 836 160 14 700
Fragments arranged by size:
836 700 160 14
ClaI AT'CG_AT
Cuts at: 0 209 1710
Size: 209 1501
EaeI y'GGCC_r
Cuts at: 0 962 1109 1395 1573 1683 1710
Size: 962 147 286 178 110 27
Fragments arranged by size:
962 286 178 147 110 27
EagI C'GGCC_G
Cuts at: 0 1573 1683 1710
Size: 1573 110 27
Fragments arranged by size:
1573 110 27
EarI CTCTTCn'nnn_
Cuts at: 0 382 680 1710
Size: 382 298 1030
Fragments arranged by size:
1030 382 298
EcoICRI GAG'CTC
Cuts at: 0 540 1710
Size: 540 1170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 221 990 1710
Size: 176 45 769 720
Fragments arranged by size:
769 720 176 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 349 466 1710
Size: 55 294 117 1244
Fragments arranged by size:
1244 294 117 55
EcoRV GAT'ATC
Cuts at: 0 609 1710
Size: 609 1101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 303 1056 1710
Size: 303 753 654
Fragments arranged by size:
753 654 303
HaeII r_GCGC'y
Cuts at: 0 1675 1710
Size: 1675 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 53 62 70 85 102 322 354
Size: 30 23 9 8 15 17 220 32
Cuts at: 354 775 807 1069 1101 1710
Size: 421 32 262 32 609
Fragments arranged by size:
609 421 262 220 32 32 32 30
23 17 15 9 8
HincII GTy'rAC
Cuts at: 0 483 1710
Size: 483 1227
HpaI GTT'AAC
Cuts at: 0 483 1710
Size: 483 1227
KasI G'GCGC_C
Cuts at: 0 1671 1710
Size: 1671 39
MfeI C'AATT_G
Cuts at: 0 738 1025 1710
Size: 738 287 685
Fragments arranged by size:
738 685 287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1448 1710
Size: 617 831 262
Fragments arranged by size:
831 617 262
MscI TGG'CCA
Cuts at: 0 964 1111 1710
Size: 964 147 599
Fragments arranged by size:
964 599 147
MslI CAynn'nnrTG
Cuts at: 0 304 431 1630 1710
Size: 304 127 1199 80
Fragments arranged by size:
1199 304 127 80
MspA1I CmG'CkG
Cuts at: 0 30 781 968 1710
Size: 30 751 187 742
Fragments arranged by size:
751 742 187 30
NarI GG'CG_CC
Cuts at: 0 1672 1710
Size: 1672 38
NspI r_CATG'y
Cuts at: 0 415 636 1477 1494 1710
Size: 415 221 841 17 216
Fragments arranged by size:
841 415 221 216 17
PasI CC'CwG_GG
Cuts at: 0 1386 1710
Size: 1386 324
PflMI CCAn_nnn'nTGG
Cuts at: 0 1140 1710
Size: 1140 570
PfoI T'CCnGG_A
Cuts at: 0 153 1710
Size: 153 1557
PmlI CAC'GTG
Cuts at: 0 1380 1710
Size: 1380 330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 1710
Size: 691 32 987
Fragments arranged by size:
987 691 32
PsiI TTA'TAA
Cuts at: 0 509 1710
Size: 509 1201
PstI C_TGCA'G
Cuts at: 0 980 1414 1710
Size: 980 434 296
Fragments arranged by size:
980 434 296
PvuII CAG'CTG
Cuts at: 0 781 1710
Size: 781 929
SacI G_AGCT'C
Cuts at: 0 542 1710
Size: 542 1168
SacII CC_GC'GG
Cuts at: 0 31 1710
Size: 31 1679
SapI GCTCTTCn'nnn_
Cuts at: 0 680 1710
Size: 680 1030
SfcI C'TryA_G
Cuts at: 0 976 1410 1710
Size: 976 434 300
Fragments arranged by size:
976 434 300
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 662 1710
Size: 662 1048
SfoI GGC'GCC
Cuts at: 0 1673 1710
Size: 1673 37
SmlI C'TyrA_G
Cuts at: 0 584 1710
Size: 584 1126
SphI G_CATG'C
Cuts at: 0 1477 1710
Size: 1477 233
StuI AGG'CCT
Cuts at: 0 355 1710
Size: 355 1355
StyI C'CwwG_G
Cuts at: 0 9 270 887 1483 1710
Size: 9 261 617 596 227
Fragments arranged by size:
617 596 261 227 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 439 1642 1710
Size: 439 1203 68
Fragments arranged by size:
1203 439 68
TatI w'GTAC_w
Cuts at: 0 669 1289 1710
Size: 669 620 421
Fragments arranged by size:
669 620 421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 24 56 1035 1067 1710
Size: 24 32 979 32 643
Fragments arranged by size:
979 643 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 439 1225 1429 1710
Size: 439 786 204 281
Fragments arranged by size:
786 439 281 204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 1024 1119 1710
Size: 186 838 95 591
Fragments arranged by size:
838 591 186 95
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
ClaI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoRV
FalI HaeII Hin4I HincII HpaI KasI MfeI MmeI
MscI MslI MspA1I NarI NspI PasI PflMI PfoI
PmlI PpiI PsiI PstI PvuII SacI SacII SapI
SfcI SfiI SfoI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BfrBI BmgBI BmtI BplI BsaI BsiWI BsmI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI CspCI
DraI DraIII DrdI EciI EcoO109I EcoRI FseI FspI
FspAI HindIII KpnI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI