Restriction map for
pF1KSDB0027
(Linear) (Six-base) MAPSORT of: KIBB0027.seq Check: 2749 from: 1 to: 2937
KIBB0027.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AatII G_ACGT'C
Cuts at: 0 1421 2937
Size: 1421 1516
AccI GT'mk_AC
Cuts at: 0 2090 2937
Size: 2090 847
AclI AA'CG_TT
Cuts at: 0 1082 2937
Size: 1082 1855
AfeI AGC'GCT
Cuts at: 0 237 2098 2937
Size: 237 1861 839
Fragments arranged by size:
1861 839 237
AflIII A'CryG_T
Cuts at: 0 2606 2819 2937
Size: 2606 213 118
Fragments arranged by size:
2606 213 118
AhdI GACnn_n'nnGTC
Cuts at: 0 336 2937
Size: 336 2601
AleI CACnn'nnGTG
Cuts at: 0 2110 2937
Size: 2110 827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 1540 2937
Size: 947 593 1397
Fragments arranged by size:
1397 947 593
AlwNI CAG_nnn'CTG
Cuts at: 0 303 315 894 1408 1605 1959 2937
Size: 303 12 579 514 197 354 978
Fragments arranged by size:
978 579 514 354 303 197 12
ApaI G_GGCC'C
Cuts at: 0 18 888 2937
Size: 18 870 2049
Fragments arranged by size:
2049 870 18
ApaLI G'TGCA_C
Cuts at: 0 2658 2937
Size: 2658 279
ApoI r'AATT_y
Cuts at: 0 1386 2937
Size: 1386 1551
AvaI C'yCGr_G
Cuts at: 0 66 649 955 1492 2739 2937
Size: 66 583 306 537 1247 198
Fragments arranged by size:
1247 583 537 306 198 66
BamHI G'GATC_C
Cuts at: 0 5 2937
Size: 5 2932
BanI G'GyrC_C
Cuts at: 0 1531 1563 1812 1903 2407 2625 2937
Size: 1531 32 249 91 504 218 312
Fragments arranged by size:
1531 504 312 249 218 91 32
BanII G_rGCy'C
Cuts at: 0 18 73 201 224 723 888 1994 2087
Size: 18 55 128 23 499 165 1106 93
Cuts at: 2087 2326 2800 2937
Size: 239 474 137
Fragments arranged by size:
1106 499 474 239 165 137 128 93
55 23 18
BbeI G_GCGC'C
Cuts at: 0 1535 2937
Size: 1535 1402
BbsI GAAGACnn'nnnn_
Cuts at: 0 746 1425 2009 2125 2937
Size: 746 679 584 116 812
Fragments arranged by size:
812 746 679 584 116
BbvCI CC'TCA_GC
Cuts at: 0 1541 1822 2937
Size: 1541 281 1115
Fragments arranged by size:
1541 1115 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2709 2743 2937
Size: 2709 34 194
Fragments arranged by size:
2709 194 34
BglI GCCn_nnn'nGGC
Cuts at: 0 187 196 1093 2455 2600 2937
Size: 187 9 897 1362 145 337
Fragments arranged by size:
1362 897 337 187 145 9
BglII A'GATC_T
Cuts at: 0 731 2937
Size: 731 2206
BlpI GC'TnA_GC
Cuts at: 0 1322 1401 1651 2209 2937
Size: 1322 79 250 558 728
Fragments arranged by size:
1322 728 558 250 79
Bme1580I G_kGCm'C
Cuts at: 0 18 888 984 1568 1817 2410 2614 2662
Size: 18 870 96 584 249 593 204 48
Cuts at: 2662 2937
Size: 275
Fragments arranged by size:
870 593 584 275 249 204 96 48
18
BmgBI CAC'GTC
Cuts at: 0 2663 2937
Size: 2663 274
BmrI ACTGGGnnnn_n'
Cuts at: 0 1677 2937
Size: 1677 1260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2448 2715 2937
Size: 2448 267 222
Fragments arranged by size:
2448 267 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1807 2047 2937
Size: 1807 240 890
Fragments arranged by size:
1807 890 240
Bpu10I CC'TnA_GC
Cuts at: 0 1541 1577 1822 2129 2156 2937
Size: 1541 36 245 307 27 781
Fragments arranged by size:
1541 781 307 245 36 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 469 1111 1207 1684 1978 2890 2937
Size: 424 45 642 96 477 294 912 47
Fragments arranged by size:
912 642 477 424 294 96 47 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 215 222 2336 2937
Size: 215 7 2114 601
Fragments arranged by size:
2114 601 215 7
BsaAI yAC'GTr
Cuts at: 0 2241 2607 2937
Size: 2241 366 330
Fragments arranged by size:
2241 366 330
BsaBI GATnn'nnATC
Cuts at: 0 1174 2937
Size: 1174 1763
BsaHI Gr'CG_yC
Cuts at: 0 767 1418 1532 1895 2937
Size: 767 651 114 363 1042
Fragments arranged by size:
1042 767 651 363 114
BsaWI w'CCGG_w
Cuts at: 0 839 2937
Size: 839 2098
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2020 2050 2937
Size: 2020 30 887
Fragments arranged by size:
2020 887 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 567 1252 1471 2704 2937
Size: 567 685 219 1233 233
Fragments arranged by size:
1233 685 567 233 219
BseYI C'CCAG_C
Cuts at: 0 232 662 1527 2147 2937
Size: 232 430 865 620 790
Fragments arranged by size:
865 790 620 430 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1394 1785 2134 2527 2773 2937
Size: 388 1006 391 349 393 246 164
Fragments arranged by size:
1006 393 391 388 349 246 164
BsiEI CG_ry'CG
Cuts at: 0 87 2595 2937
Size: 87 2508 342
Fragments arranged by size:
2508 342 87
BsiHKAI G_wGCw'C
Cuts at: 0 926 1402 1994 2326 2662 2937
Size: 926 476 592 332 336 275
Fragments arranged by size:
926 592 476 336 332 275
BsmI GAATG_Cn'
Cuts at: 0 602 2937
Size: 602 2335
Bsp1286I G_dGCh'C
Cuts at: 0 18 73 201 224 723 888 926 984
Size: 18 55 128 23 499 165 38 58
Cuts at: 984 1402 1568 1817 1994 2087 2326 2410 2614
Size: 418 166 249 177 93 239 84 204
Cuts at: 2614 2662 2800 2937
Size: 48 138 137
Fragments arranged by size:
499 418 249 239 204 177 166 165
138 137 128 93 84 58 55 48
38 23 18
BspHI T'CATG_A
Cuts at: 0 791 2186 2270 2937
Size: 791 1395 84 667
Fragments arranged by size:
1395 791 667 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 683 1580 1886 2937
Size: 300 383 897 306 1051
Fragments arranged by size:
1051 897 383 306 300
BsrBI CCG'CTC
Cuts at: 0 1326 1631 2937
Size: 1326 305 1306
Fragments arranged by size:
1326 1306 305
BsrDI GCAATG_nn'
Cuts at: 0 1535 2275 2937
Size: 1535 740 662
Fragments arranged by size:
1535 740 662
BsrGI T'GTAC_A
Cuts at: 0 2141 2937
Size: 2141 796
BssSI C'ACGA_G
Cuts at: 0 1315 2937
Size: 1315 1622
BstAPI GCAn_nnn'nTGC
Cuts at: 0 303 315 2937
Size: 303 12 2622
Fragments arranged by size:
2622 303 12
BstYI r'GATC_y
Cuts at: 0 5 731 836 2202 2937
Size: 5 726 105 1366 735
Fragments arranged by size:
1366 735 726 105 5
Bsu36I CC'TnA_GG
Cuts at: 0 192 2309 2668 2937
Size: 192 2117 359 269
Fragments arranged by size:
2117 359 269 192
BtgI C'CryG_G
Cuts at: 0 185 275 2709 2937
Size: 185 90 2434 228
Fragments arranged by size:
2434 228 185 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 584 603 837 1620 2789 2937
Size: 584 19 234 783 1169 148
Fragments arranged by size:
1169 783 584 234 148 19
BtsI GCAGTG_nn'
Cuts at: 0 822 1306 1776 2563 2937
Size: 822 484 470 787 374
Fragments arranged by size:
822 787 484 470 374
DraIII CAC_nnn'GTG
Cuts at: 0 1671 2937
Size: 1671 1266
DrdI GACnn_nn'nnGTC
Cuts at: 0 1843 2937
Size: 1843 1094
EaeI y'GGCC_r
Cuts at: 0 84 179 1118 1910 2045 2219 2592 2937
Size: 84 95 939 792 135 174 373 345
Fragments arranged by size:
939 792 373 345 174 135 95 84
EagI C'GGCC_G
Cuts at: 0 84 2592 2937
Size: 84 2508 345
Fragments arranged by size:
2508 345 84
EarI CTCTTCn'nnn_
Cuts at: 0 1489 1787 2020 2937
Size: 1489 298 233 917
Fragments arranged by size:
1489 917 298 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 134 142 2937
Size: 134 8 2795
Fragments arranged by size:
2795 134 8
EcoICRI GAG'CTC
Cuts at: 0 1992 2324 2937
Size: 1992 332 613
Fragments arranged by size:
1992 613 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1807 2047 2937
Size: 1807 240 890
Fragments arranged by size:
1807 890 240
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 1222 2221 2937
Size: 307 915 999 716
Fragments arranged by size:
999 915 716 307
EcoO109I rG'GnC_Cy
Cuts at: 0 15 109 300 312 779 1061 2010 2226
Size: 15 94 191 12 467 282 949 216
Cuts at: 2226 2937
Size: 711
Fragments arranged by size:
949 711 467 282 216 191 94 15
12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1299 1638 2031 2937
Size: 1299 339 393 906
Fragments arranged by size:
1299 906 393 339
HaeII r_GCGC'y
Cuts at: 0 239 895 1535 2100 2937
Size: 239 656 640 565 837
Fragments arranged by size:
837 656 640 565 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 562 594 1360 1392 2020 2052
Size: 322 32 208 32 766 32 628 32
Cuts at: 2052 2112 2144 2937
Size: 60 32 793
Fragments arranged by size:
793 766 628 322 208 60 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2736 2937
Size: 2736 201
KasI G'GCGC_C
Cuts at: 0 1531 2937
Size: 1531 1406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 1589 2937
Size: 1403 186 1348
Fragments arranged by size:
1403 1348 186
MscI TGG'CCA
Cuts at: 0 2047 2937
Size: 2047 890
MslI CAynn'nnrTG
Cuts at: 0 1180 1522 2110 2479 2719 2937
Size: 1180 342 588 369 240 218
Fragments arranged by size:
1180 588 369 342 240 218
MspA1I CmG'CkG
Cuts at: 0 101 187 321 1405 2213 2250 2711 2901
Size: 101 86 134 1084 808 37 461 190
Cuts at: 2901 2937
Size: 36
Fragments arranged by size:
1084 808 461 190 134 101 86 37
36
NarI GG'CG_CC
Cuts at: 0 1532 2937
Size: 1532 1405
NcoI C'CATG_G
Cuts at: 0 275 2937
Size: 275 2662
NspI r_CATG'y
Cuts at: 0 1032 1053 1554 1695 2295 2478 2823 2937
Size: 1032 21 501 141 600 183 345 114
Fragments arranged by size:
1032 600 501 345 183 141 114 21
PciI A'CATG_T
Cuts at: 0 2819 2937
Size: 2819 118
PflMI CCAn_nnn'nTGG
Cuts at: 0 281 1671 2622 2937
Size: 281 1390 951 315
Fragments arranged by size:
1390 951 315 281
PfoI T'CCnGG_A
Cuts at: 0 1436 1733 1832 2937
Size: 1436 297 99 1105
Fragments arranged by size:
1436 1105 297 99
PmlI CAC'GTG
Cuts at: 0 2241 2937
Size: 2241 696
PpuMI rG'GwC_Cy
Cuts at: 0 109 312 1061 2010 2937
Size: 109 203 749 949 927
Fragments arranged by size:
949 927 749 203 109
PshAI GACnn'nnGTC
Cuts at: 0 441 2937
Size: 441 2496
PsiI TTA'TAA
Cuts at: 0 2828 2937
Size: 2828 109
PspOMI G'GGCC_C
Cuts at: 0 14 884 2937
Size: 14 870 2053
Fragments arranged by size:
2053 870 14
PstI C_TGCA'G
Cuts at: 0 308 320 641 728 812 829 1058 1771
Size: 308 12 321 87 84 17 229 713
Cuts at: 1771 2060 2639 2937
Size: 289 579 298
Fragments arranged by size:
713 579 321 308 298 289 229 87
84 17 12
PvuII CAG'CTG
Cuts at: 0 101 321 2250 2937
Size: 101 220 1929 687
Fragments arranged by size:
1929 687 220 101
SacI G_AGCT'C
Cuts at: 0 1994 2326 2937
Size: 1994 332 611
Fragments arranged by size:
1994 611 332
SacII CC_GC'GG
Cuts at: 0 188 2712 2937
Size: 188 2524 225
Fragments arranged by size:
2524 225 188
SanDI GG'GwC_CC
Cuts at: 0 109 2937
Size: 109 2828
ScaI AGT'ACT
Cuts at: 0 1198 2937
Size: 1198 1739
SexAI A'CCwGG_T
Cuts at: 0 2525 2937
Size: 2525 412
SfcI C'TryA_G
Cuts at: 0 304 316 637 724 808 825 1054 1767
Size: 304 12 321 87 84 17 229 713
Cuts at: 1767 1919 2056 2635 2780 2937
Size: 152 137 579 145 157
Fragments arranged by size:
713 579 321 304 229 157 152 145
137 87 84 17 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 187 2937
Size: 187 2750
SfoI GGC'GCC
Cuts at: 0 1533 2937
Size: 1533 1404
SmaI CCC'GGG
Cuts at: 0 2741 2937
Size: 2741 196
SmlI C'TyrA_G
Cuts at: 0 439 484 1090 1222 1699 1993 2905 2937
Size: 439 45 606 132 477 294 912 32
Fragments arranged by size:
912 606 477 439 294 132 45 32
SphI G_CATG'C
Cuts at: 0 1554 2295 2478 2937
Size: 1554 741 183 459
Fragments arranged by size:
1554 741 459 183
SspI AAT'ATT
Cuts at: 0 906 2937
Size: 906 2031
StuI AGG'CCT
Cuts at: 0 141 1096 1478 1621 1798 2937
Size: 141 955 382 143 177 1139
Fragments arranged by size:
1139 955 382 177 143 141
StyI C'CwwG_G
Cuts at: 0 275 626 1622 2653 2937
Size: 275 351 996 1031 284
Fragments arranged by size:
1031 996 351 284 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1488 1659 2161 2221 2571 2643 2781 2937
Size: 1488 171 502 60 350 72 138 156
Fragments arranged by size:
1488 502 350 171 156 138 72 60
TatI w'GTAC_w
Cuts at: 0 1046 1196 2141 2937
Size: 1046 150 945 796
Fragments arranged by size:
1046 945 796 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1403 1435 2937
Size: 1403 32 1502
Fragments arranged by size:
1502 1403 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1905 2565 2595 2937
Size: 1905 660 30 342
Fragments arranged by size:
1905 660 342 30
XmaI C'CCGG_G
Cuts at: 0 2739 2937
Size: 2739 198
XmnI GAAnn'nnTTC
Cuts at: 0 868 2937
Size: 868 2069
ZraI GAC'GTC
Cuts at: 0 1419 2937
Size: 1419 1518
Enzymes that do cut:
AatII AccI AclI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI
BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NcoI NspI PciI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PstI PvuII SacI
SacII SanDI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AcuI AflII AgeI AloI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BmtI BsiWI
BsmBI BspEI BsrFI BssHII BstBI BstEII BstXI BstZ17I
ClaI CspCI DraI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PpiI
PspXI PsrI PvuI RsrII SalI SapI SbfI SgfI
SgrAI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI