Restriction map for
pF1KSDB0025
(Linear) (Six-base) MAPSORT of: KIBB0025.seq Check: 6768 from: 1 to: 1692
KIBB0025.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 99 1692
Size: 99 1593
AatII G_ACGT'C
Cuts at: 0 83 1692
Size: 83 1609
AccI GT'mk_AC
Cuts at: 0 83 1412 1692
Size: 83 1329 280
Fragments arranged by size:
1329 280 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1581 1628 1692
Size: 698 883 47 64
Fragments arranged by size:
883 698 64 47
AflIII A'CryG_T
Cuts at: 0 740 1692
Size: 740 952
AhdI GACnn_n'nnGTC
Cuts at: 0 719 1692
Size: 719 973
AleI CACnn'nnGTG
Cuts at: 0 232 1692
Size: 232 1460
AlwNI CAG_nnn'CTG
Cuts at: 0 1408 1692
Size: 1408 284
ApaLI G'TGCA_C
Cuts at: 0 1498 1692
Size: 1498 194
BamHI G'GATC_C
Cuts at: 0 26 1652 1692
Size: 26 1626 40
Fragments arranged by size:
1626 40 26
BanI G'GyrC_C
Cuts at: 0 277 411 888 1369 1692
Size: 277 134 477 481 323
Fragments arranged by size:
481 477 323 277 134
BanII G_rGCy'C
Cuts at: 0 125 1692
Size: 125 1567
BbsI GAAGACnn'nnnn_
Cuts at: 0 1022 1344 1444 1692
Size: 1022 322 100 248
Fragments arranged by size:
1022 322 248 100
BciVI GTATCCnnnnn_n'
Cuts at: 0 1172 1692
Size: 1172 520
BclI T'GATC_A
Cuts at: 0 287 1692
Size: 287 1405
BglI GCCn_nnn'nGGC
Cuts at: 0 1607 1692
Size: 1607 85
BlpI GC'TnA_GC
Cuts at: 0 1145 1692
Size: 1145 547
Bme1580I G_kGCm'C
Cuts at: 0 414 1372 1502 1692
Size: 414 958 130 190
Fragments arranged by size:
958 414 190 130
BmgBI CAC'GTC
Cuts at: 0 300 1692
Size: 300 1392
Bpu10I CC'TnA_GC
Cuts at: 0 605 1692
Size: 605 1087
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 802 1692
Size: 310 492 890
Fragments arranged by size:
890 492 310
BsaAI yAC'GTr
Cuts at: 0 230 393 1692
Size: 230 163 1299
Fragments arranged by size:
1299 230 163
BsaBI GATnn'nnATC
Cuts at: 0 31 1692
Size: 31 1661
BsaHI Gr'CG_yC
Cuts at: 0 80 1349 1692
Size: 80 1269 343
Fragments arranged by size:
1269 343 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 32 62 876 906 1692
Size: 32 30 814 30 786
Fragments arranged by size:
814 786 32 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 679 1097 1277 1469 1652 1692
Size: 679 418 180 192 183 40
Fragments arranged by size:
679 418 192 183 180 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 803 1072 1692
Size: 48 755 269 620
Fragments arranged by size:
755 620 269 48
BsiHKAI G_wGCw'C
Cuts at: 0 125 1502 1692
Size: 125 1377 190
Fragments arranged by size:
1377 190 125
BsmI GAATG_Cn'
Cuts at: 0 228 1692
Size: 228 1464
Bsp1286I G_dGCh'C
Cuts at: 0 125 414 1372 1502 1692
Size: 125 289 958 130 190
Fragments arranged by size:
958 289 190 130 125
BspHI T'CATG_A
Cuts at: 0 125 218 479 929 1692
Size: 125 93 261 450 763
Fragments arranged by size:
763 450 261 125 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 1242 1692
Size: 99 1143 450
Fragments arranged by size:
1143 450 99
BsrDI GCAATG_nn'
Cuts at: 0 701 1692
Size: 701 991
BsrFI r'CCGG_y
Cuts at: 0 977 1372 1692
Size: 977 395 320
Fragments arranged by size:
977 395 320
BsrGI T'GTAC_A
Cuts at: 0 497 1692
Size: 497 1195
BssSI C'ACGA_G
Cuts at: 0 70 1692
Size: 70 1622
BstEII G'GTnAC_C
Cuts at: 0 1311 1692
Size: 1311 381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 1692
Size: 234 1458
BstYI r'GATC_y
Cuts at: 0 26 1028 1652 1692
Size: 26 1002 624 40
Fragments arranged by size:
1002 624 40 26
Bsu36I CC'TnA_GG
Cuts at: 0 1390 1692
Size: 1390 302
BtgI C'CryG_G
Cuts at: 0 249 1296 1692
Size: 249 1047 396
Fragments arranged by size:
1047 396 249
BtsI GCAGTG_nn'
Cuts at: 0 815 1692
Size: 815 877
ClaI AT'CG_AT
Cuts at: 0 1619 1692
Size: 1619 73
DraI TTT'AAA
Cuts at: 0 1445 1692
Size: 1445 247
DraIII CAC_nnn'GTG
Cuts at: 0 407 1302 1692
Size: 407 895 390
Fragments arranged by size:
895 407 390
DrdI GACnn_nn'nnGTC
Cuts at: 0 367 1692
Size: 367 1325
EaeI y'GGCC_r
Cuts at: 0 294 356 593 941 1121 1692
Size: 294 62 237 348 180 571
Fragments arranged by size:
571 348 294 237 180 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 820 1692
Size: 820 872
EcoICRI GAG'CTC
Cuts at: 0 123 1692
Size: 123 1569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1581 1628 1692
Size: 698 883 47 64
Fragments arranged by size:
883 698 64 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 1667 1692
Size: 34 1633 25
Fragments arranged by size:
1633 34 25
EcoO109I rG'GnC_Cy
Cuts at: 0 492 1215 1394 1578 1692
Size: 492 723 179 184 114
Fragments arranged by size:
723 492 184 179 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1035 1692
Size: 1035 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 642 674 705 737 1644 1676 1692
Size: 642 32 31 32 907 32 16
Fragments arranged by size:
907 642 32 32 32 31 16
HincII GTy'rAC
Cuts at: 0 462 738 1692
Size: 462 276 954
Fragments arranged by size:
954 462 276
HindIII A'AGCT_T
Cuts at: 0 1147 1692
Size: 1147 545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 1071 1160 1692
Size: 252 819 89 532
Fragments arranged by size:
819 532 252 89
MscI TGG'CCA
Cuts at: 0 943 1692
Size: 943 749
MslI CAynn'nnrTG
Cuts at: 0 232 283 1692
Size: 232 51 1409
Fragments arranged by size:
1409 232 51
MspA1I CmG'CkG
Cuts at: 0 775 1692
Size: 775 917
NcoI C'CATG_G
Cuts at: 0 249 1692
Size: 249 1443
NspI r_CATG'y
Cuts at: 0 504 826 1290 1692
Size: 504 322 464 402
Fragments arranged by size:
504 464 402 322
PasI CC'CwG_GG
Cuts at: 0 1504 1692
Size: 1504 188
PflMI CCAn_nnn'nTGG
Cuts at: 0 111 1692
Size: 111 1581
PfoI T'CCnGG_A
Cuts at: 0 1185 1665 1692
Size: 1185 480 27
Fragments arranged by size:
1185 480 27
PmlI CAC'GTG
Cuts at: 0 230 393 1692
Size: 230 163 1299
Fragments arranged by size:
1299 230 163
PpuMI rG'GwC_Cy
Cuts at: 0 492 1215 1394 1578 1692
Size: 492 723 179 184 114
Fragments arranged by size:
723 492 184 179 114
PvuII CAG'CTG
Cuts at: 0 775 1692
Size: 775 917
SacI G_AGCT'C
Cuts at: 0 125 1692
Size: 125 1567
SanDI GG'GwC_CC
Cuts at: 0 1215 1692
Size: 1215 477
SexAI A'CCwGG_T
Cuts at: 0 403 731 1692
Size: 403 328 961
Fragments arranged by size:
961 403 328
StyI C'CwwG_G
Cuts at: 0 143 249 257 365 416 1692
Size: 143 106 8 108 51 1276
Fragments arranged by size:
1276 143 108 106 51 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 214 429 1692
Size: 82 132 215 1263
Fragments arranged by size:
1263 215 132 82
TatI w'GTAC_w
Cuts at: 0 497 1692
Size: 497 1195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 155 187 876 908 1692
Size: 32 32 91 32 689 32 784
Fragments arranged by size:
784 689 91 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1408 1692
Size: 1408 284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 577 1692
Size: 165 412 1115
Fragments arranged by size:
1115 412 165
ZraI GAC'GTC
Cuts at: 0 81 1692
Size: 81 1611
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlwNI
ApaLI BamHI BanI BanII BbsI BciVI BclI BglI
BlpI Bme1580I BmgBI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI ClaI DraI DraIII DrdI EaeI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NspI PasI PflMI
PfoI PmlI PpuMI PvuII SacI SanDI SexAI StyI
TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AlfI AloI ApaI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BfrBI BglII BmrI BmtI BplI BpmI
BpuEI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BstAPI BstBI BstZ17I BtgZI CspCI EagI EarI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI XmnI