Restriction map for
pF1KB0019
(Linear) (Six-base) MAPSORT of: KIBB0019.seq Check: 578 from: 1 to: 8529
KIBB0019.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 7953 8529
Size: 7953 576
AccI GT'mk_AC
Cuts at: 0 690 1983 2722 6954 7110 8529
Size: 690 1293 739 4232 156 1419
Fragments arranged by size:
4232 1419 1293 739 690 156
Acc65I G'GTAC_C
Cuts at: 0 613 8529
Size: 613 7916
AclI AA'CG_TT
Cuts at: 0 3435 8529
Size: 3435 5094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 469 2981 3464 3869 3968 4013 4049
Size: 67 402 2512 483 405 99 45 36
Cuts at: 4049 5764 6122 8378 8529
Size: 1715 358 2256 151
Fragments arranged by size:
2512 2256 1715 483 405 402 358 151
99 67 45 36
AflII C'TTAA_G
Cuts at: 0 6757 7353 8529
Size: 6757 596 1176
Fragments arranged by size:
6757 1176 596
AflIII A'CryG_T
Cuts at: 0 2836 5359 5361 7770 8529
Size: 2836 2523 2 2409 759
Fragments arranged by size:
2836 2523 2409 759 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1406 1646 5276 8529
Size: 1406 240 3630 3253
Fragments arranged by size:
3630 3253 1406 240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8472 8504 8529
Size: 8472 32 25
Fragments arranged by size:
8472 32 25
AlwNI CAG_nnn'CTG
Cuts at: 0 1321 3892 4453 5839 7081 8529
Size: 1321 2571 561 1386 1242 1448
Fragments arranged by size:
2571 1448 1386 1321 1242 561
ApaLI G'TGCA_C
Cuts at: 0 1832 8529
Size: 1832 6697
ApoI r'AATT_y
Cuts at: 0 528 659 1113 1647 1779 2823 4057 5047
Size: 528 131 454 534 132 1044 1234 990
Cuts at: 5047 5442 5856 6291 6324 6497 6623 7855 8529
Size: 395 414 435 33 173 126 1232 674
Fragments arranged by size:
1234 1232 1044 990 674 534 528 454
435 414 395 173 132 131 126 33
AseI AT'TA_AT
Cuts at: 0 3362 5183 8529
Size: 3362 1821 3346
Fragments arranged by size:
3362 3346 1821
AvaI C'yCGr_G
Cuts at: 0 1118 1136 6674 7676 8529
Size: 1118 18 5538 1002 853
Fragments arranged by size:
5538 1118 1002 853 18
AvrII C'CTAG_G
Cuts at: 0 8368 8529
Size: 8368 161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6038 6071 8529
Size: 6038 33 2458
Fragments arranged by size:
6038 2458 33
BanI G'GyrC_C
Cuts at: 0 613 7941 8529
Size: 613 7328 588
Fragments arranged by size:
7328 613 588
BanII G_rGCy'C
Cuts at: 0 5975 8529
Size: 5975 2554
BbsI GAAGACnn'nnnn_
Cuts at: 0 505 1048 1619 2711 3626 5258 5457 7250
Size: 505 543 571 1092 915 1632 199 1793
Cuts at: 7250 7566 8529
Size: 316 963
Fragments arranged by size:
1793 1632 1092 963 915 571 543 505
316 199
BciVI GTATCCnnnnn_n'
Cuts at: 0 7827 8529
Size: 7827 702
BclI T'GATC_A
Cuts at: 0 18 6456 8529
Size: 18 6438 2073
Fragments arranged by size:
6438 2073 18
BfrBI ATG'CAT
Cuts at: 0 2038 5180 8529
Size: 2038 3142 3349
Fragments arranged by size:
3349 3142 2038
BglI GCCn_nnn'nGGC
Cuts at: 0 1546 8529
Size: 1546 6983
BglII A'GATC_T
Cuts at: 0 1334 2636 2872 6222 8008 8017 8529
Size: 1334 1302 236 3350 1786 9 512
Fragments arranged by size:
3350 1786 1334 1302 512 236 9
BlpI GC'TnA_GC
Cuts at: 0 770 2297 8529
Size: 770 1527 6232
Fragments arranged by size:
6232 1527 770
Bme1580I G_kGCm'C
Cuts at: 0 1836 5037 8529
Size: 1836 3201 3492
Fragments arranged by size:
3492 3201 1836
BmrI ACTGGGnnnn_n'
Cuts at: 0 4253 5796 6046 8529
Size: 4253 1543 250 2483
Fragments arranged by size:
4253 2483 1543 250
BmtI G_CTAG'C
Cuts at: 0 1006 8529
Size: 1006 7523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 752 7206 8529
Size: 752 6454 1323
Fragments arranged by size:
6454 1323 752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 341 4472 4562 5033 6749 7088 7427
Size: 269 72 4131 90 471 1716 339 339
Cuts at: 7427 8405 8529
Size: 978 124
Fragments arranged by size:
4131 1716 978 471 339 339 269 124
90 72
Bpu10I CC'TnA_GC
Cuts at: 0 4400 8492 8529
Size: 4400 4092 37
Fragments arranged by size:
4400 4092 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 2104 2223 3244 5084 5990 6048 6151
Size: 430 1674 119 1021 1840 906 58 103
Cuts at: 6151 6705 8283 8529
Size: 554 1578 246
Fragments arranged by size:
1840 1674 1578 1021 906 554 430 246
119 103 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 3465 4129 6246 7595 8529
Size: 3465 664 2117 1349 934
Fragments arranged by size:
3465 2117 1349 934 664
BsaAI yAC'GTr
Cuts at: 0 4765 5362 8529
Size: 4765 597 3167
Fragments arranged by size:
4765 3167 597
BsaBI GATnn'nnATC
Cuts at: 0 306 6906 8529
Size: 306 6600 1623
Fragments arranged by size:
6600 1623 306
BsaWI w'CCGG_w
Cuts at: 0 1865 5877 8529
Size: 1865 4012 2652
Fragments arranged by size:
4012 2652 1865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5006 5036 6792 6822 8529
Size: 5006 30 1756 30 1707
Fragments arranged by size:
5006 1756 1707 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1054 2490 4316 5042 6708 6830 8529
Size: 1054 1436 1826 726 1666 122 1699
Fragments arranged by size:
1826 1699 1666 1436 1054 726 122
BseYI C'CCAG_C
Cuts at: 0 2494 3000 6938 8529
Size: 2494 506 3938 1591
Fragments arranged by size:
3938 2494 1591 506
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 881 1172 3689 5258 8529
Size: 348 533 291 2517 1569 3271
Fragments arranged by size:
3271 2517 1569 533 348 291
BsiHKAI G_wGCw'C
Cuts at: 0 642 1584 1836 3244 4098 4524 7652 8529
Size: 642 942 252 1408 854 426 3128 877
Fragments arranged by size:
3128 1408 942 877 854 642 426 252
BsmI GAATG_Cn'
Cuts at: 0 5182 7164 8529
Size: 5182 1982 1365
Fragments arranged by size:
5182 1982 1365
Bsp1286I G_dGCh'C
Cuts at: 0 642 1584 1836 3244 4098 4524 5037 5975
Size: 642 942 252 1408 854 426 513 938
Cuts at: 5975 7652 8529
Size: 1677 877
Fragments arranged by size:
1677 1408 942 938 877 854 642 513
426 252
BspHI T'CATG_A
Cuts at: 0 972 1278 8529
Size: 972 306 7251
Fragments arranged by size:
7251 972 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 625 1614 6942 7953 8529
Size: 625 989 5328 1011 576
Fragments arranged by size:
5328 1011 989 625 576
BsrBI CCG'CTC
Cuts at: 0 774 8529
Size: 774 7755
BsrDI GCAATG_nn'
Cuts at: 0 424 1092 1400 1421 1791 6143 8529
Size: 424 668 308 21 370 4352 2386
Fragments arranged by size:
4352 2386 668 424 370 308 21
BsrFI r'CCGG_y
Cuts at: 0 754 8529
Size: 754 7775
BsrGI T'GTAC_A
Cuts at: 0 4841 8529
Size: 4841 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3892 4375 4417 8529
Size: 3892 483 42 4112
Fragments arranged by size:
4112 3892 483 42
BstBI TT'CG_AA
Cuts at: 0 6708 8529
Size: 6708 1821
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2670 6201 6877 8529
Size: 2670 3531 676 1652
Fragments arranged by size:
3531 2670 1652 676
BstYI r'GATC_y
Cuts at: 0 301 1334 2636 2872 3951 6222 6907 8008
Size: 301 1033 1302 236 1079 2271 685 1101
Cuts at: 8008 8017 8411 8529
Size: 9 394 118
Fragments arranged by size:
2271 1302 1101 1079 1033 685 394 301
236 118 9
BstZ17I GTA'TAC
Cuts at: 0 691 8529
Size: 691 7838
Bsu36I CC'TnA_GG
Cuts at: 0 7074 7893 8529
Size: 7074 819 636
Fragments arranged by size:
7074 819 636
BtgI C'CryG_G
Cuts at: 0 4475 4915 8144 8529
Size: 4475 440 3229 385
Fragments arranged by size:
4475 3229 440 385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 610 8529
Size: 610 7919
BtsI GCAGTG_nn'
Cuts at: 0 2645 3648 3980 4236 8133 8529
Size: 2645 1003 332 256 3897 396
Fragments arranged by size:
3897 2645 1003 396 332 256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3323 3358 8529
Size: 3323 35 5171
Fragments arranged by size:
5171 3323 35
DraI TTT'AAA
Cuts at: 0 2891 3542 5396 8529
Size: 2891 651 1854 3133
Fragments arranged by size:
3133 2891 1854 651
DraIII CAC_nnn'GTG
Cuts at: 0 8320 8529
Size: 8320 209
EaeI y'GGCC_r
Cuts at: 0 6972 8401 8529
Size: 6972 1429 128
Fragments arranged by size:
6972 1429 128
EarI CTCTTCn'nnn_
Cuts at: 0 354 1373 2258 2508 3461 3497 8529
Size: 354 1019 885 250 953 36 5032
Fragments arranged by size:
5032 1019 953 885 354 250 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 269 341 469 2981 3464 3869 3968
Size: 67 202 72 128 2512 483 405 99
Cuts at: 3968 4013 4049 4472 4562 5033 5764 6122 6749
Size: 45 36 423 90 471 731 358 627
Cuts at: 6749 7088 7427 8378 8405 8529
Size: 339 339 951 27 124
Fragments arranged by size:
2512 951 731 627 483 471 423 405
358 339 339 202 128 124 99 90
72 67 45 36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5075 5546 6115 6785 8529
Size: 5075 471 569 670 1744
Fragments arranged by size:
5075 1744 670 569 471
EcoO109I rG'GnC_Cy
Cuts at: 0 4683 7427 8529
Size: 4683 2744 1102
Fragments arranged by size:
4683 2744 1102
EcoRI G'AATT_C
Cuts at: 0 659 6497 8529
Size: 659 5838 2032
Fragments arranged by size:
5838 2032 659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3236 3398 5531 7727 8529
Size: 3236 162 2133 2196 802
Fragments arranged by size:
3236 2196 2133 802 162
HaeII r_GCGC'y
Cuts at: 0 7575 8499 8529
Size: 7575 924 30
Fragments arranged by size:
7575 924 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 903 935 973 1005 1081 1113
Size: 718 32 153 32 38 32 76 32
Cuts at: 1113 2413 2445 3637 3669 3701 7604 7636 8403
Size: 1300 32 1192 32 32 3903 32 767
Cuts at: 8403 8435 8529
Size: 32 94
Fragments arranged by size:
3903 1300 1192 767 718 153 94 76
38 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 0 5616 7322 8529
Size: 5616 1706 1207
Fragments arranged by size:
5616 1706 1207
HindIII A'AGCT_T
Cuts at: 0 2241 2517 6380 8529
Size: 2241 276 3863 2149
Fragments arranged by size:
3863 2241 2149 276
KpnI G_GTAC'C
Cuts at: 0 617 8529
Size: 617 7912
MfeI C'AATT_G
Cuts at: 0 562 8529
Size: 562 7967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1677 2798 3291 5729 8529
Size: 1677 1121 493 2438 2800
Fragments arranged by size:
2800 2438 1677 1121 493
MslI CAynn'nnrTG
Cuts at: 0 1085 1388 3041 8529
Size: 1085 303 1653 5488
Fragments arranged by size:
5488 1653 1085 303
MspA1I CmG'CkG
Cuts at: 0 1028 3847 3946 4833 8529
Size: 1028 2819 99 887 3696
Fragments arranged by size:
3696 2819 1028 887 99
NdeI CA'TA_TG
Cuts at: 0 15 3038 3629 8529
Size: 15 3023 591 4900
Fragments arranged by size:
4900 3023 591 15
NheI G'CTAG_C
Cuts at: 0 1002 8529
Size: 1002 7527
NruI TCG'CGA
Cuts at: 0 993 8529
Size: 993 7536
NsiI A_TGCA'T
Cuts at: 0 2040 5182 8529
Size: 2040 3142 3347
Fragments arranged by size:
3347 3142 2040
NspI r_CATG'y
Cuts at: 0 1393 2042 2118 2840 4888 6368 6663 7774
Size: 1393 649 76 722 2048 1480 295 1111
Cuts at: 7774 8529
Size: 755
Fragments arranged by size:
2048 1480 1393 1111 755 722 649 295
76
PciI A'CATG_T
Cuts at: 0 2836 7770 8529
Size: 2836 4934 759
Fragments arranged by size:
4934 2836 759
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 3277 4231 8529
Size: 627 2650 954 4298
Fragments arranged by size:
4298 2650 954 627
PfoI T'CCnGG_A
Cuts at: 0 2607 6699 8529
Size: 2607 4092 1830
Fragments arranged by size:
4092 2607 1830
PmlI CAC'GTG
Cuts at: 0 5362 8529
Size: 5362 3167
PpuMI rG'GwC_Cy
Cuts at: 0 4683 7427 8529
Size: 4683 2744 1102
Fragments arranged by size:
4683 2744 1102
PshAI GACnn'nnGTC
Cuts at: 0 2026 8529
Size: 2026 6503
PsiI TTA'TAA
Cuts at: 0 4252 6584 8529
Size: 4252 2332 1945
Fragments arranged by size:
4252 2332 1945
PspXI vC'TCGA_Gb
Cuts at: 0 7676 8529
Size: 7676 853
PstI C_TGCA'G
Cuts at: 0 9 2172 2652 4013 4413 4425 8529
Size: 9 2163 480 1361 400 12 4104
Fragments arranged by size:
4104 2163 1361 480 400 12 9
PvuII CAG'CTG
Cuts at: 0 1028 3847 3946 4833 8529
Size: 1028 2819 99 887 3696
Fragments arranged by size:
3696 2819 1028 887 99
SapI GCTCTTCn'nnn_
Cuts at: 0 1373 8529
Size: 1373 7156
ScaI AGT'ACT
Cuts at: 0 140 390 4485 7056 7875 8529
Size: 140 250 4095 2571 819 654
Fragments arranged by size:
4095 2571 819 654 250 140
SexAI A'CCwGG_T
Cuts at: 0 5243 8529
Size: 5243 3286
SfcI C'TryA_G
Cuts at: 0 5 1414 1476 1823 2168 2483 2648 4009
Size: 5 1409 62 347 345 315 165 1361
Cuts at: 4009 4409 4421 5072 7541 8264 8529
Size: 400 12 651 2469 723 265
Fragments arranged by size:
2469 1409 1361 723 651 400 347 345
315 265 165 62 12 5
SmaI CCC'GGG
Cuts at: 0 1120 8529
Size: 1120 7409
SmlI C'TyrA_G
Cuts at: 0 409 2119 2238 3259 5099 6005 6063 6166
Size: 409 1710 119 1021 1840 906 58 103
Cuts at: 6166 6674 6720 6757 7353 7676 8298 8529
Size: 508 46 37 596 323 622 231
Fragments arranged by size:
1840 1710 1021 906 622 596 508 409
323 231 119 103 58 46 37
SphI G_CATG'C
Cuts at: 0 2118 8529
Size: 2118 6411
SspI AAT'ATT
Cuts at: 0 2223 3091 8529
Size: 2223 868 5438
Fragments arranged by size:
5438 2223 868
StuI AGG'CCT
Cuts at: 0 2199 8529
Size: 2199 6330
StyI C'CwwG_G
Cuts at: 0 929 2342 5459 7036 8368 8529
Size: 929 1413 3117 1577 1332 161
Fragments arranged by size:
3117 1577 1413 1332 929 161
SwaI ATTT'AAAT
Cuts at: 0 5396 8529
Size: 5396 3133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4119 5292 6193 7962 8529
Size: 4119 1173 901 1769 567
Fragments arranged by size:
4119 1769 1173 901 567
TatI w'GTAC_w
Cuts at: 0 138 388 2207 3214 4159 4483 4841 5086
Size: 138 250 1819 1007 945 324 358 245
Cuts at: 5086 6520 6724 7054 7873 8529
Size: 1434 204 330 819 656
Fragments arranged by size:
1819 1434 1007 945 819 656 358 330
324 250 245 204 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 917 949 3543 3575 6792 6824 8529
Size: 917 32 2594 32 3217 32 1705
Fragments arranged by size:
3217 2594 1705 917 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 5245 8529
Size: 5245 3284
XbaI T'CTAG_A
Cuts at: 0 5592 6373 6898 6910 7249 7405 7609 8529
Size: 5592 781 525 12 339 156 204 920
Fragments arranged by size:
5592 920 781 525 339 204 156 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 561 936 1078 3274 5133 8142 8529
Size: 561 375 142 2196 1859 3009 387
Fragments arranged by size:
3009 2196 1859 561 387 375 142
XhoI C'TCGA_G
Cuts at: 0 6674 7676 8529
Size: 6674 1002 853
Fragments arranged by size:
6674 1002 853
XmaI C'CCGG_G
Cuts at: 0 1118 8529
Size: 1118 7411
XmnI GAAnn'nnTTC
Cuts at: 0 2616 5577 6072 7257 8529
Size: 2616 2961 495 1185 1272
Fragments arranged by size:
2961 2616 1272 1185 495
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AloI AlwNI ApaLI ApoI AseI AvaI AvrII BaeI
BanI BanII BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KpnI MfeI MmeI MslI MspA1I NdeI NheI NruI
NsiI NspI PciI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PstI PvuII SapI ScaI SexAI SfcI
SmaI SmlI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI ApaI AscI AsiSI
BamHI BbeI BbvCI BcgI BmgBI BsaHI BsiEI BsiWI
BsmBI BspEI BssHII BssSI BstEII ClaI DrdI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HpaI KasI
MluI MscI NaeI NarI NcoI NgoMIV NotI PacI
PasI PmeI PpiI PspOMI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI ZraI