Restriction map for
pF1KB0015
(Linear) (Six-base) MAPSORT of: KIBB0015.seq Check: 4535 from: 1 to: 4773
KIBB0015.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3159 3612 4773
Size: 3159 453 1161
Fragments arranged by size:
3159 1161 453
AatII G_ACGT'C
Cuts at: 0 1715 2114 4773
Size: 1715 399 2659
Fragments arranged by size:
2659 1715 399
AccI GT'mk_AC
Cuts at: 0 2227 4773
Size: 2227 2546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 2318 2644 2691 2866 3631 3886 3898
Size: 265 2053 326 47 175 765 255 12
Cuts at: 3898 4138 4773
Size: 240 635
Fragments arranged by size:
2053 765 635 326 265 255 240 175
47 12
AflII C'TTAA_G
Cuts at: 0 1351 4773
Size: 1351 3422
AflIII A'CryG_T
Cuts at: 0 1376 2230 2435 4773
Size: 1376 854 205 2338
Fragments arranged by size:
2338 1376 854 205
AhdI GACnn_n'nnGTC
Cuts at: 0 254 2019 3465 4773
Size: 254 1765 1446 1308
Fragments arranged by size:
1765 1446 1308 254
AleI CACnn'nnGTG
Cuts at: 0 67 220 4412 4773
Size: 67 153 4192 361
Fragments arranged by size:
4192 361 153 67
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 461 1607 2005 3047 4773
Size: 461 1146 398 1042 1726
Fragments arranged by size:
1726 1146 1042 461 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1933 1965 4773
Size: 1933 32 2808
Fragments arranged by size:
2808 1933 32
AlwNI CAG_nnn'CTG
Cuts at: 0 675 1563 3537 4773
Size: 675 888 1974 1236
Fragments arranged by size:
1974 1236 888 675
ApaI G_GGCC'C
Cuts at: 0 2879 3261 4464 4514 4773
Size: 2879 382 1203 50 259
Fragments arranged by size:
2879 1203 382 259 50
ApaLI G'TGCA_C
Cuts at: 0 637 1419 4773
Size: 637 782 3354
Fragments arranged by size:
3354 782 637
ApoI r'AATT_y
Cuts at: 0 34 55 359 2362 2949 3244 4726 4773
Size: 34 21 304 2003 587 295 1482 47
Fragments arranged by size:
2003 1482 587 304 295 47 34 21
AvaI C'yCGr_G
Cuts at: 0 695 2303 4513 4559 4721 4773
Size: 695 1608 2210 46 162 52
Fragments arranged by size:
2210 1608 695 162 52 46
AvrII C'CTAG_G
Cuts at: 0 1059 4773
Size: 1059 3714
BamHI G'GATC_C
Cuts at: 0 1127 1855 4773
Size: 1127 728 2918
Fragments arranged by size:
2918 1127 728
BanI G'GyrC_C
Cuts at: 0 1780 3171 3952 4634 4773
Size: 1780 1391 781 682 139
Fragments arranged by size:
1780 1391 781 682 139
BanII G_rGCy'C
Cuts at: 0 1229 2879 3261 3653 3887 4144 4464 4514
Size: 1229 1650 382 392 234 257 320 50
Cuts at: 4514 4773
Size: 259
Fragments arranged by size:
1650 1229 392 382 320 259 257 234
50
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 539 1707 3327 3862 4773
Size: 253 286 1168 1620 535 911
Fragments arranged by size:
1620 1168 911 535 286 253
BbvCI CC'TCA_GC
Cuts at: 0 1619 3768 4773
Size: 1619 2149 1005
Fragments arranged by size:
2149 1619 1005
BciVI GTATCCnnnnn_n'
Cuts at: 0 3246 4773
Size: 3246 1527
BclI T'GATC_A
Cuts at: 0 120 4773
Size: 120 4653
BfrBI ATG'CAT
Cuts at: 0 1093 3052 3748 4773
Size: 1093 1959 696 1025
Fragments arranged by size:
1959 1093 1025 696
BglI GCCn_nnn'nGGC
Cuts at: 0 1200 3772 4643 4773
Size: 1200 2572 871 130
Fragments arranged by size:
2572 1200 871 130
BglII A'GATC_T
Cuts at: 0 326 1032 1691 4146 4773
Size: 326 706 659 2455 627
Fragments arranged by size:
2455 706 659 627 326
BlpI GC'TnA_GC
Cuts at: 0 3652 3985 4773
Size: 3652 333 788
Fragments arranged by size:
3652 788 333
Bme1580I G_kGCm'C
Cuts at: 0 641 1423 1827 2879 3261 3739 3955 4464
Size: 641 782 404 1052 382 478 216 509
Cuts at: 4464 4514 4639 4773
Size: 50 125 134
Fragments arranged by size:
1052 782 641 509 478 404 382 216
134 125 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 513 4313 4467 4773
Size: 513 3800 154 306
Fragments arranged by size:
3800 513 306 154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 888 4773
Size: 888 3885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 2846 3145 3571 3737 4153 4773
Size: 1138 1708 299 426 166 416 620
Fragments arranged by size:
1708 1138 620 426 416 299 166
Bpu10I CC'TnA_GC
Cuts at: 0 1619 3768 3776 4773
Size: 1619 2149 8 997
Fragments arranged by size:
2149 1619 997 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3988 4333 4773
Size: 3988 345 440
Fragments arranged by size:
3988 440 345
BsaI GGTCTCn'nnnn_
Cuts at: 0 574 4773
Size: 574 4199
BsaAI yAC'GTr
Cuts at: 0 1377 1872 2220 3141 4773
Size: 1377 495 348 921 1632
Fragments arranged by size:
1632 1377 921 495 348
BsaHI Gr'CG_yC
Cuts at: 0 1712 2111 3758 4773
Size: 1712 399 1647 1015
Fragments arranged by size:
1712 1647 1015 399
BsaWI w'CCGG_w
Cuts at: 0 1625 3368 4685 4773
Size: 1625 1743 1317 88
Fragments arranged by size:
1743 1625 1317 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1008 1038 1933 1963 3544 3574 4773
Size: 1008 30 895 30 1581 30 1199
Fragments arranged by size:
1581 1199 1008 895 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 418 777 1099 1418 1957 1962 2212
Size: 341 77 359 322 319 539 5 250
Cuts at: 2212 2297 2321 3073 3429 4519 4716 4773
Size: 85 24 752 356 1090 197 57
Fragments arranged by size:
1090 752 539 359 356 341 322 319
250 197 85 77 57 24 5
BseYI C'CCAG_C
Cuts at: 0 620 2641 2878 3183 3391 3480 3956 4773
Size: 620 2021 237 305 208 89 476 817
Fragments arranged by size:
2021 817 620 476 305 237 208 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1762 2242 3091 3550 4076 4773
Size: 1762 480 849 459 526 697
Fragments arranged by size:
1762 849 697 526 480 459
BsiHKAI G_wGCw'C
Cuts at: 0 100 641 1122 1423 2467 3653 3887 4313
Size: 100 541 481 301 1044 1186 234 426
Cuts at: 4313 4773
Size: 460
Fragments arranged by size:
1186 1044 541 481 460 426 301 234
100
BsmI GAATG_Cn'
Cuts at: 0 1095 2882 3385 3750 4773
Size: 1095 1787 503 365 1023
Fragments arranged by size:
1787 1095 1023 503 365
BsmBI CGTCTCn'nnnn_
Cuts at: 0 298 859 2157 2303 4773
Size: 298 561 1298 146 2470
Fragments arranged by size:
2470 1298 561 298 146
Bsp1286I G_dGCh'C
Cuts at: 0 100 641 1122 1229 1423 1827 2467 2879
Size: 100 541 481 107 194 404 640 412
Cuts at: 2879 3261 3653 3739 3887 3955 4144 4313 4464
Size: 382 392 86 148 68 189 169 151
Cuts at: 4464 4514 4639 4773
Size: 50 125 134
Fragments arranged by size:
640 541 481 412 404 392 382 194
189 169 151 148 134 125 107 100
86 68 50
BspEI T'CCGG_A
Cuts at: 0 3368 4685 4773
Size: 3368 1317 88
Fragments arranged by size:
3368 1317 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 913 3113 3159 3612 4773
Size: 913 2200 46 453 1161
Fragments arranged by size:
2200 1161 913 453 46
BsrBI CCG'CTC
Cuts at: 0 747 4773
Size: 747 4026
BsrDI GCAATG_nn'
Cuts at: 0 499 1337 1499 4116 4713 4773
Size: 499 838 162 2617 597 60
Fragments arranged by size:
2617 838 597 499 162 60
BsrFI r'CCGG_y
Cuts at: 0 654 4773
Size: 654 4119
BsrGI T'GTAC_A
Cuts at: 0 4178 4773
Size: 4178 595
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1515 1520 3204 4773
Size: 1515 5 1684 1569
Fragments arranged by size:
1684 1569 1515 5
BstEII G'GTnAC_C
Cuts at: 0 1180 4773
Size: 1180 3593
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1815 2754 4240 4773
Size: 1815 939 1486 533
Fragments arranged by size:
1815 1486 939 533
BstYI r'GATC_y
Cuts at: 0 326 1032 1127 1560 1667 1691 1855 3147
Size: 326 706 95 433 107 24 164 1292
Cuts at: 3147 4146 4652 4773
Size: 999 506 121
Fragments arranged by size:
1292 999 706 506 433 326 164 121
107 95 24
BstZ17I GTA'TAC
Cuts at: 0 2228 4773
Size: 2228 2545
Bsu36I CC'TnA_GG
Cuts at: 0 1082 2981 4773
Size: 1082 1899 1792
Fragments arranged by size:
1899 1792 1082
BtgI C'CryG_G
Cuts at: 0 218 3569 4757 4773
Size: 218 3351 1188 16
Fragments arranged by size:
3351 1188 218 16
BtsI GCAGTG_nn'
Cuts at: 0 4 506 582 1270 2283 2462 3968 4057
Size: 4 502 76 688 1013 179 1506 89
Cuts at: 4057 4773
Size: 716
Fragments arranged by size:
1506 1013 716 688 502 179 89 76
4
DraIII CAC_nnn'GTG
Cuts at: 0 3848 4773
Size: 3848 925
EaeI y'GGCC_r
Cuts at: 0 915 1451 1646 2067 2474 3710 4773
Size: 915 536 195 421 407 1236 1063
Fragments arranged by size:
1236 1063 915 536 421 407 195
EarI CTCTTCn'nnn_
Cuts at: 0 88 1355 2125 4132 4417 4773
Size: 88 1267 770 2007 285 356
Fragments arranged by size:
2007 1267 770 356 285 88
EcoICRI GAG'CTC
Cuts at: 0 3651 3885 4773
Size: 3651 234 888
Fragments arranged by size:
3651 888 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1138 2318 2644 2691 2846 2866 3145
Size: 265 873 1180 326 47 155 20 279
Cuts at: 3145 3571 3631 3737 3886 3898 4138 4153 4773
Size: 426 60 106 149 12 240 15 620
Fragments arranged by size:
1180 873 620 426 326 279 265 240
155 149 106 60 47 20 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3724 4773
Size: 3724 1049
EcoO109I rG'GnC_Cy
Cuts at: 0 274 1063 1830 2875 3257 3258 3764 4461
Size: 274 789 767 1045 382 1 506 697
Cuts at: 4461 4510 4773
Size: 49 263
Fragments arranged by size:
1045 789 767 697 506 382 274 263
49 1
EcoRI G'AATT_C
Cuts at: 0 2949 3244 4773
Size: 2949 295 1529
Fragments arranged by size:
2949 1529 295
EcoRV GAT'ATC
Cuts at: 0 396 4773
Size: 396 4377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2346 4110 4143 4773
Size: 2346 1764 33 630
Fragments arranged by size:
2346 1764 630 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 240 272 1078 1110 1354 1386 1768 1800
Size: 240 32 806 32 244 32 382 32
Cuts at: 1800 2005 2037 2159 2191 2276 2308 2656 2688
Size: 205 32 122 32 85 32 348 32
Cuts at: 2688 3220 3252 3451 3483 4131 4138 4163 4170
Size: 532 32 199 32 648 7 25 7
Cuts at: 4170 4680 4712 4773
Size: 510 32 61
Fragments arranged by size:
806 648 532 510 382 348 244 240
205 199 122 85 61 32 32 32
32 32 32 32 32 32 32 32
25 7 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 2190 2573 4773
Size: 242 1948 383 2200
Fragments arranged by size:
2200 1948 383 242
MscI TGG'CCA
Cuts at: 0 917 1648 2069 2476 4773
Size: 917 731 421 407 2297
Fragments arranged by size:
2297 917 731 421 407
MslI CAynn'nnrTG
Cuts at: 0 67 220 1642 1648 1813 1987 3517 4412
Size: 67 153 1422 6 165 174 1530 895
Cuts at: 4412 4661 4773
Size: 249 112
Fragments arranged by size:
1530 1422 895 249 174 165 153 112
67 6
MspA1I CmG'CkG
Cuts at: 0 675 1158 1587 2844 3549 3772 3960 4363
Size: 675 483 429 1257 705 223 188 403
Cuts at: 4363 4622 4773
Size: 259 151
Fragments arranged by size:
1257 705 675 483 429 403 259 223
188 151
NcoI C'CATG_G
Cuts at: 0 218 3569 4773
Size: 218 3351 1204
Fragments arranged by size:
3351 1204 218
NdeI CA'TA_TG
Cuts at: 0 66 2793 4658 4773
Size: 66 2727 1865 115
Fragments arranged by size:
2727 1865 115 66
NsiI A_TGCA'T
Cuts at: 0 1095 3054 3750 4773
Size: 1095 1959 696 1023
Fragments arranged by size:
1959 1095 1023 696
NspI r_CATG'y
Cuts at: 0 986 1248 1518 2234 2420 2439 3307 4773
Size: 986 262 270 716 186 19 868 1466
Fragments arranged by size:
1466 986 868 716 270 262 186 19
PasI CC'CwG_GG
Cuts at: 0 1827 4773
Size: 1827 2946
PciI A'CATG_T
Cuts at: 0 2230 2435 4773
Size: 2230 205 2338
Fragments arranged by size:
2338 2230 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 419 1177 4773
Size: 419 758 3596
Fragments arranged by size:
3596 758 419
PfoI T'CCnGG_A
Cuts at: 0 1205 1706 3815 4130 4773
Size: 1205 501 2109 315 643
Fragments arranged by size:
2109 1205 643 501 315
PmlI CAC'GTG
Cuts at: 0 1377 2220 4773
Size: 1377 843 2553
Fragments arranged by size:
2553 1377 843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1933 1965 4773
Size: 1933 32 2808
Fragments arranged by size:
2808 1933 32
PpuMI rG'GwC_Cy
Cuts at: 0 1063 4773
Size: 1063 3710
PshAI GACnn'nnGTC
Cuts at: 0 173 4773
Size: 173 4600
PspOMI G'GGCC_C
Cuts at: 0 2875 3257 4460 4510 4773
Size: 2875 382 1203 50 263
Fragments arranged by size:
2875 1203 382 263 50
PspXI vC'TCGA_Gb
Cuts at: 0 2303 4773
Size: 2303 2470
PstI C_TGCA'G
Cuts at: 0 1163 1397 1525 1586 1596 3603 3623 4154
Size: 1163 234 128 61 10 2007 20 531
Cuts at: 4154 4773
Size: 619
Fragments arranged by size:
2007 1163 619 531 234 128 61 20
10
PvuII CAG'CTG
Cuts at: 0 675 1158 2844 3549 3960 4622 4773
Size: 675 483 1686 705 411 662 151
Fragments arranged by size:
1686 705 675 662 483 411 151
SacI G_AGCT'C
Cuts at: 0 3653 3887 4773
Size: 3653 234 886
Fragments arranged by size:
3653 886 234
SapI GCTCTTCn'nnn_
Cuts at: 0 88 1355 4132 4773
Size: 88 1267 2777 641
Fragments arranged by size:
2777 1267 641 88
SbfI CC_TGCA'GG
Cuts at: 0 1397 4773
Size: 1397 3376
SfcI C'TryA_G
Cuts at: 0 104 1159 1393 1521 1582 1592 3599 3619
Size: 104 1055 234 128 61 10 2007 20
Cuts at: 3619 4150 4773
Size: 531 623
Fragments arranged by size:
2007 1055 623 531 234 128 104 61
20 10
SmaI CCC'GGG
Cuts at: 0 697 4561 4773
Size: 697 3864 212
Fragments arranged by size:
3864 697 212
SmlI C'TyrA_G
Cuts at: 0 1351 2303 4003 4348 4773
Size: 1351 952 1700 345 425
Fragments arranged by size:
1700 1351 952 425 345
SphI G_CATG'C
Cuts at: 0 1248 1518 3307 4773
Size: 1248 270 1789 1466
Fragments arranged by size:
1789 1466 1248 270
StuI AGG'CCT
Cuts at: 0 1399 1487 2776 4673 4773
Size: 1399 88 1289 1897 100
Fragments arranged by size:
1897 1399 1289 100 88
StyI C'CwwG_G
Cuts at: 0 218 449 947 992 1059 1142 1841 2064
Size: 218 231 498 45 67 83 699 223
Cuts at: 2064 3569 3977 4233 4613 4640 4773
Size: 1505 408 256 380 27 133
Fragments arranged by size:
1505 699 498 408 380 256 231 223
218 133 83 67 45 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 572 3970 4246 4773
Size: 572 3398 276 527
Fragments arranged by size:
3398 572 527 276
TatI w'GTAC_w
Cuts at: 0 81 1770 2327 2432 3873 4178 4469 4773
Size: 81 1689 557 105 1441 305 291 304
Fragments arranged by size:
1689 1441 557 305 304 291 105 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 889 921 1112 1144 2082 2114 4773
Size: 889 32 191 32 938 32 2659
Fragments arranged by size:
2659 938 889 191 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 925 4773
Size: 925 3848
XhoI C'TCGA_G
Cuts at: 0 2303 4773
Size: 2303 2470
XmaI C'CCGG_G
Cuts at: 0 695 4559 4773
Size: 695 3864 214
Fragments arranged by size:
3864 695 214
XmnI GAAnn'nnTTC
Cuts at: 0 1323 2248 2632 2657 4126 4773
Size: 1323 925 384 25 1469 647
Fragments arranged by size:
1469 1323 925 647 384 25
ZraI GAC'GTC
Cuts at: 0 1713 2112 4773
Size: 1713 399 2661
Fragments arranged by size:
2661 1713 399
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SapI SbfI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AscI AseI AsiSI BaeI
BbeI BcgI BmgBI BmtI BsaBI BsiEI BsiWI BspHI
BssHII BssSI BstBI BtgZI ClaI CspCI DraI DrdI
EagI EciI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PmeI PsiI PsrI PvuI
RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI