Restriction map for
pF1KSDB0010
(Linear) (Six-base) MAPSORT of: KIBB0010.seq Check: 8039 from: 1 to: 1857
KIBB0010.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1337 1857
Size: 1337 520
Acc65I G'GTAC_C
Cuts at: 0 130 1857
Size: 130 1727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1269 1857
Size: 551 718 588
Fragments arranged by size:
718 588 551
AfeI AGC'GCT
Cuts at: 0 1111 1444 1857
Size: 1111 333 413
Fragments arranged by size:
1111 413 333
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 1857
Size: 173 32 1652
Fragments arranged by size:
1652 173 32
AlwNI CAG_nnn'CTG
Cuts at: 0 145 948 1017 1468 1857
Size: 145 803 69 451 389
Fragments arranged by size:
803 451 389 145 69
ApaI G_GGCC'C
Cuts at: 0 101 338 508 1857
Size: 101 237 170 1349
Fragments arranged by size:
1349 237 170 101
ApoI r'AATT_y
Cuts at: 0 1628 1857
Size: 1628 229
AvaI C'yCGr_G
Cuts at: 0 703 1238 1857
Size: 703 535 619
Fragments arranged by size:
703 619 535
BamHI G'GATC_C
Cuts at: 0 1436 1857
Size: 1436 421
BanI G'GyrC_C
Cuts at: 0 130 764 953 1857
Size: 130 634 189 904
Fragments arranged by size:
904 634 189 130
BanII G_rGCy'C
Cuts at: 0 101 204 338 508 938 1621 1728 1857
Size: 101 103 134 170 430 683 107 129
Fragments arranged by size:
683 430 170 134 129 107 103 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 703 1251 1857
Size: 703 548 606
Fragments arranged by size:
703 606 548
BbvCI CC'TCA_GC
Cuts at: 0 301 1857
Size: 301 1556
BciVI GTATCCnnnnn_n'
Cuts at: 0 35 1053 1857
Size: 35 1018 804
Fragments arranged by size:
1018 804 35
BglI GCCn_nnn'nGGC
Cuts at: 0 685 1602 1857
Size: 685 917 255
Fragments arranged by size:
917 685 255
BglII A'GATC_T
Cuts at: 0 1505 1857
Size: 1505 352
BlpI GC'TnA_GC
Cuts at: 0 824 1857
Size: 824 1033
Bme1580I G_kGCm'C
Cuts at: 0 101 338 508 956 1106 1857
Size: 101 237 170 448 150 751
Fragments arranged by size:
751 448 237 170 150 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 1857
Size: 55 1802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 131 1857
Size: 131 1726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1241 1393 1401 1628 1772 1857
Size: 530 711 152 8 227 144 85
Fragments arranged by size:
711 530 227 152 144 85 8
Bpu10I CC'TnA_GC
Cuts at: 0 301 692 1169 1857
Size: 301 391 477 688
Fragments arranged by size:
688 477 391 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1018 1857
Size: 1006 12 839
Fragments arranged by size:
1006 839 12
BsaAI yAC'GTr
Cuts at: 0 642 1857
Size: 642 1215
BsaWI w'CCGG_w
Cuts at: 0 38 265 1857
Size: 38 227 1592
Fragments arranged by size:
1592 227 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 1078 1108 1292 1322 1857
Size: 529 30 519 30 184 30 535
Fragments arranged by size:
535 529 519 184 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 366 478 550 580 946 967 1316 1319
Size: 366 112 72 30 366 21 349 3
Cuts at: 1319 1775 1857
Size: 456 82
Fragments arranged by size:
456 366 366 349 112 82 72 30
21 3
BseYI C'CCAG_C
Cuts at: 0 289 948 1051 1131 1857
Size: 289 659 103 80 726
Fragments arranged by size:
726 659 289 103 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1857
Size: 1012 845
BsiHKAI G_wGCw'C
Cuts at: 0 938 1857
Size: 938 919
BsiWI C'GTAC_G
Cuts at: 0 151 1857
Size: 151 1706
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1557 1857
Size: 1557 300
Bsp1286I G_dGCh'C
Cuts at: 0 101 204 338 508 938 956 1106 1621
Size: 101 103 134 170 430 18 150 515
Cuts at: 1621 1728 1857
Size: 107 129
Fragments arranged by size:
515 430 170 150 134 129 107 103
101 18
BspHI T'CATG_A
Cuts at: 0 1001 1857
Size: 1001 856
BsrDI GCAATG_nn'
Cuts at: 0 224 1537 1857
Size: 224 1313 320
Fragments arranged by size:
1313 320 224
BsrFI r'CCGG_y
Cuts at: 0 848 1857
Size: 848 1009
BssHII G'CGCG_C
Cuts at: 0 69 1857
Size: 69 1788
BssSI C'ACGA_G
Cuts at: 0 841 1857
Size: 841 1016
BstAPI GCAn_nnn'nTGC
Cuts at: 0 960 1468 1857
Size: 960 508 389
Fragments arranged by size:
960 508 389
BstEII G'GTnAC_C
Cuts at: 0 1203 1390 1857
Size: 1203 187 467
Fragments arranged by size:
1203 467 187
BstXI CCAn_nnnn'nTGG
Cuts at: 0 498 1587 1857
Size: 498 1089 270
Fragments arranged by size:
1089 498 270
BstYI r'GATC_y
Cuts at: 0 807 1436 1505 1857
Size: 807 629 69 352
Fragments arranged by size:
807 629 352 69
BstZ17I GTA'TAC
Cuts at: 0 1338 1857
Size: 1338 519
Bsu36I CC'TnA_GG
Cuts at: 0 349 1214 1857
Size: 349 865 643
Fragments arranged by size:
865 643 349
BtgI C'CryG_G
Cuts at: 0 683 1857
Size: 683 1174
BtsI GCAGTG_nn'
Cuts at: 0 240 1857
Size: 240 1617
EaeI y'GGCC_r
Cuts at: 0 27 677 1594 1718 1857
Size: 27 650 917 124 139
Fragments arranged by size:
917 650 139 124 27
EarI CTCTTCn'nnn_
Cuts at: 0 527 1857
Size: 527 1330
EcoICRI GAG'CTC
Cuts at: 0 936 1857
Size: 936 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 551 1241 1269 1393 1401 1628 1772
Size: 530 21 690 28 124 8 227 144
Cuts at: 1772 1857
Size: 85
Fragments arranged by size:
690 530 227 144 124 85 28 21
8
EcoO109I rG'GnC_Cy
Cuts at: 0 97 98 189 325 334 504 505 699
Size: 97 1 91 136 9 170 1 194
Cuts at: 699 1603 1749 1857
Size: 904 146 108
Fragments arranged by size:
904 194 170 146 136 108 97 91
9 1 1
EcoRI G'AATT_C
Cuts at: 0 1628 1857
Size: 1628 229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1846 1857
Size: 1846 11
HaeII r_GCGC'y
Cuts at: 0 849 1113 1446 1857
Size: 849 264 333 411
Fragments arranged by size:
849 411 333 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 529 561 1857
Size: 127 32 370 32 1296
Fragments arranged by size:
1296 370 127 32 32
KpnI G_GTAC'C
Cuts at: 0 134 1857
Size: 134 1723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 419 479 1822 1857
Size: 341 78 60 1343 35
Fragments arranged by size:
1343 341 78 60 35
MscI TGG'CCA
Cuts at: 0 679 1720 1857
Size: 679 1041 137
Fragments arranged by size:
1041 679 137
MslI CAynn'nnrTG
Cuts at: 0 860 1354 1857
Size: 860 494 503
Fragments arranged by size:
860 503 494
MspA1I CmG'CkG
Cuts at: 0 948 966 996 1014 1857
Size: 948 18 30 18 843
Fragments arranged by size:
948 843 30 18 18
NaeI GCC'GGC
Cuts at: 0 850 1857
Size: 850 1007
NcoI C'CATG_G
Cuts at: 0 683 1857
Size: 683 1174
NdeI CA'TA_TG
Cuts at: 0 423 857 1857
Size: 423 434 1000
Fragments arranged by size:
1000 434 423
NgoMIV G'CCGG_C
Cuts at: 0 848 1857
Size: 848 1009
NruI TCG'CGA
Cuts at: 0 179 1857
Size: 179 1678
NspI r_CATG'y
Cuts at: 0 66 77 901 1498 1857
Size: 66 11 824 597 359
Fragments arranged by size:
824 597 359 66 11
PasI CC'CwG_GG
Cuts at: 0 85 331 1296 1650 1857
Size: 85 246 965 354 207
Fragments arranged by size:
965 354 246 207 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 1432 1857
Size: 1432 425
PpuMI rG'GwC_Cy
Cuts at: 0 189 699 1857
Size: 189 510 1158
Fragments arranged by size:
1158 510 189
PshAI GACnn'nnGTC
Cuts at: 0 407 1857
Size: 407 1450
PspOMI G'GGCC_C
Cuts at: 0 97 334 504 1857
Size: 97 237 170 1353
Fragments arranged by size:
1353 237 170 97
PstI C_TGCA'G
Cuts at: 0 209 965 1068 1457 1464 1857
Size: 209 756 103 389 7 393
Fragments arranged by size:
756 393 389 209 103 7
PvuII CAG'CTG
Cuts at: 0 948 966 1014 1857
Size: 948 18 48 843
Fragments arranged by size:
948 843 48 18
RsrII CG'GwC_CG
Cuts at: 0 12 1857
Size: 12 1845
SacI G_AGCT'C
Cuts at: 0 938 1857
Size: 938 919
SanDI GG'GwC_CC
Cuts at: 0 699 1857
Size: 699 1158
SbfI CC_TGCA'GG
Cuts at: 0 1457 1857
Size: 1457 400
SfcI C'TryA_G
Cuts at: 0 205 961 1064 1453 1460 1857
Size: 205 756 103 389 7 397
Fragments arranged by size:
756 397 389 205 103 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1602 1857
Size: 1602 255
SmlI C'TyrA_G
Cuts at: 0 1021 1033 1857
Size: 1021 12 824
Fragments arranged by size:
1021 824 12
SphI G_CATG'C
Cuts at: 0 66 77 1857
Size: 66 11 1780
Fragments arranged by size:
1780 66 11
StuI AGG'CCT
Cuts at: 0 1483 1857
Size: 1483 374
StyI C'CwwG_G
Cuts at: 0 103 683 1386 1752 1857
Size: 103 580 703 366 105
Fragments arranged by size:
703 580 366 105 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1563 1683 1719 1857
Size: 1563 120 36 138
Fragments arranged by size:
1563 138 120 36
TatI w'GTAC_w
Cuts at: 0 869 1857
Size: 869 988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1292 1324 1857
Size: 1078 32 182 32 533
Fragments arranged by size:
1078 533 182 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 543 1857
Size: 543 1314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 428 1713 1857
Size: 428 1285 144
Fragments arranged by size:
1285 428 144
Enzymes that do cut:
AccI Acc65I AcuI AfeI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
BsmBI Bsp1286I BspHI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NruI NspI PasI PflMI PpuMI PshAI PspOMI
PstI PvuII RsrII SacI SanDI SbfI SfcI SfiI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BclI BfrBI BmgBI BmtI BsaI BsaBI BsaHI
BsiEI BsmI BspEI BspMI BsrBI BsrGI BstBI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI NarI NheI NotI NsiI PacI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
SacII SalI SapI ScaI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
XmaI XmnI ZraI