Restriction map for
pF1KA2034
(Linear) (Six-base) MAPSORT of: KIAA2034.seq Check: 9423 from: 1 to: 6108
KIAA2034.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 129 1793 2700 6108
Size: 129 1664 907 3408
Fragments arranged by size:
3408 1664 907 129
AatII G_ACGT'C
Cuts at: 0 4742 6108
Size: 4742 1366
AccI GT'mk_AC
Cuts at: 0 1832 6108
Size: 1832 4276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1788 2545 3361 3730 5080 5839 6108
Size: 724 1064 757 816 369 1350 759 269
Fragments arranged by size:
1350 1064 816 759 757 724 369 269
AflIII A'CryG_T
Cuts at: 0 404 518 6108
Size: 404 114 5590
Fragments arranged by size:
5590 404 114
AgeI A'CCGG_T
Cuts at: 0 1004 6108
Size: 1004 5104
AhdI GACnn_n'nnGTC
Cuts at: 0 3555 3911 6108
Size: 3555 356 2197
Fragments arranged by size:
3555 2197 356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2031 4768 6108
Size: 2031 2737 1340
Fragments arranged by size:
2737 2031 1340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1684 1716 5537 5569 6108
Size: 1684 32 3821 32 539
Fragments arranged by size:
3821 1684 539 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 945 2005 2433 2754 2931 3078 3330 3501
Size: 945 1060 428 321 177 147 252 171
Cuts at: 3501 3762 3786 4140 4395 4458 4542 5541 6108
Size: 261 24 354 255 63 84 999 567
Fragments arranged by size:
1060 999 945 567 428 354 321 261
255 252 177 171 147 84 63 24
ApaI G_GGCC'C
Cuts at: 0 55 101 2748 3423 3489 3495 4629 4698
Size: 55 46 2647 675 66 6 1134 69
Cuts at: 4698 5598 6108
Size: 900 510
Fragments arranged by size:
2647 1134 900 675 510 69 66 55
46 6
ApoI r'AATT_y
Cuts at: 0 814 3118 3153 6108
Size: 814 2304 35 2955
Fragments arranged by size:
2955 2304 814 35
AvaI C'yCGr_G
Cuts at: 0 25 61 123 985 1259 2564 3488 4181
Size: 25 36 62 862 274 1305 924 693
Cuts at: 4181 4367 4622 5174 5855 6108
Size: 186 255 552 681 253
Fragments arranged by size:
1305 924 862 693 681 552 274 255
253 186 62 36 25
BanI G'GyrC_C
Cuts at: 0 21 36 127 171 507 856 1432 1781
Size: 21 15 91 44 336 349 576 349
Cuts at: 1781 3935 4307 4355 5381 6108
Size: 2154 372 48 1026 727
Fragments arranged by size:
2154 1026 727 576 372 349 349 336
91 48 44 21 15
BanII G_rGCy'C
Cuts at: 0 55 101 992 2748 2807 3367 3423 3489
Size: 55 46 891 1756 59 560 56 66
Cuts at: 3489 3495 3677 4029 4629 4698 5598 5862 6108
Size: 6 182 352 600 69 900 264 246
Fragments arranged by size:
1756 900 891 600 560 352 264 246
182 69 66 59 56 55 46 6
BbeI G_GCGC'C
Cuts at: 0 40 860 1436 3939 4311 4359 5385 6108
Size: 40 820 576 2503 372 48 1026 723
Fragments arranged by size:
2503 1026 820 723 576 372 48 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 616 3152 3479 3721 3850 5994 6108
Size: 616 2536 327 242 129 2144 114
Fragments arranged by size:
2536 2144 616 327 242 129 114
BbvCI CC'TCA_GC
Cuts at: 0 3056 3221 3763 4202 5234 5651 6108
Size: 3056 165 542 439 1032 417 457
Fragments arranged by size:
3056 1032 542 457 439 417 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1767 1801 6108
Size: 1767 34 4307
Fragments arranged by size:
4307 1767 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2563 6108
Size: 2563 3545
BglI GCCn_nnn'nGGC
Cuts at: 0 45 96 652 2848 3862 4198 4372 4702
Size: 45 51 556 2196 1014 336 174 330
Cuts at: 4702 5222 6108
Size: 520 886
Fragments arranged by size:
2196 1014 886 556 520 336 330 174
51 45
BglII A'GATC_T
Cuts at: 0 1472 1931 2474 5183 6108
Size: 1472 459 543 2709 925
Fragments arranged by size:
2709 1472 925 543 459
BlpI GC'TnA_GC
Cuts at: 0 3163 4102 4706 4723 5498 6108
Size: 3163 939 604 17 775 610
Fragments arranged by size:
3163 939 775 610 604 17
Bme1580I G_kGCm'C
Cuts at: 0 26 55 62 101 130 2748 3423 3489
Size: 26 29 7 39 29 2618 675 66
Cuts at: 3489 3495 3996 4332 4629 4647 4698 5598 5604
Size: 6 501 336 297 18 51 900 6
Cuts at: 5604 6108
Size: 504
Fragments arranged by size:
2618 900 675 504 501 336 297 66
51 39 29 29 26 18 7 6
6
BmgBI CAC'GTC
Cuts at: 0 3913 6108
Size: 3913 2195
BmrI ACTGGGnnnn_n'
Cuts at: 0 49 1249 2186 6108
Size: 49 1200 937 3922
Fragments arranged by size:
3922 1200 937 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 982 1011 1285 5205 6108
Size: 982 29 274 3920 903
Fragments arranged by size:
3920 982 903 274 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 620 745 901 977 1090 1126 1363
Size: 611 9 125 156 76 113 36 237
Cuts at: 1363 1582 2263 2308 2791 2953 2974 3106 3352
Size: 219 681 45 483 162 21 132 246
Cuts at: 3352 3574 3637 3658 3742 3829 3880 3964 4060
Size: 222 63 21 84 87 51 84 96
Cuts at: 4060 4249 4354 4447 4501 4531 4669 4699 4732
Size: 189 105 93 54 30 138 30 33
Cuts at: 4732 4846 4888 5059 5071 5248 5377 5395 5428
Size: 114 42 171 12 177 129 18 33
Cuts at: 5428 5725 5809 5851 6108
Size: 297 84 42 257
Fragments arranged by size:
681 611 483 297 257 246 237 222
219 189 177 171 162 156 138 132
129 125 114 113 105 96 93 87
84 84 84 76 63 54 51 45
42 42 36 33 33 30 30 21
21 18 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 2155 3056 3221 3763 4202 5234 5338 5651
Size: 2155 901 165 542 439 1032 104 313
Cuts at: 5651 5861 6108
Size: 210 247
Fragments arranged by size:
2155 1032 901 542 439 313 247 210
165 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2638 3070 3506 4873 5203 5650 6108
Size: 2638 432 436 1367 330 447 458
Fragments arranged by size:
2638 1367 458 447 436 432 330
BsaI GGTCTCn'nnnn_
Cuts at: 0 862 1134 2513 4147 4405 5665 6108
Size: 862 272 1379 1634 258 1260 443
Fragments arranged by size:
1634 1379 1260 862 443 272 258
BsaAI yAC'GTr
Cuts at: 0 407 519 651 4669 6108
Size: 407 112 132 4018 1439
Fragments arranged by size:
4018 1439 407 132 112
BsaHI Gr'CG_yC
Cuts at: 0 37 159 857 1433 3936 4308 4356 4466
Size: 37 122 698 576 2503 372 48 110
Cuts at: 4466 4739 5382 5571 6108
Size: 273 643 189 537
Fragments arranged by size:
2503 698 643 576 537 372 273 189
122 110 48 37
BsaWI w'CCGG_w
Cuts at: 0 1004 2123 3677 6108
Size: 1004 1119 1554 2431
Fragments arranged by size:
2431 1554 1119 1004
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 784 814 1684 1714 2965 2995
Size: 394 30 360 30 870 30 1251 30
Cuts at: 2995 3664 3694 4033 4063 6108
Size: 669 30 339 30 2045
Fragments arranged by size:
2045 1251 870 669 394 360 339 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 241 789 970 973 1585 1705 2527
Size: 238 3 548 181 3 612 120 822
Cuts at: 2527 2848 2908 2986 2989 2992 3073 3238 3241
Size: 321 60 78 3 3 81 165 3
Cuts at: 3241 3244 3325 3424 3454 3457 3625 3739 3742
Size: 3 81 99 30 3 168 114 3
Cuts at: 3742 4165 4246 4249 4330 4333 4360 4564 4666
Size: 423 81 3 81 3 27 204 102
Cuts at: 4666 4669 4687 5008 5068 5110 5173 5194 5374
Size: 3 18 321 60 42 63 21 180
Cuts at: 5374 5425 5428 5596 5668 5764 5767 5848 5857
Size: 51 3 168 72 96 3 81 9
Cuts at: 5857 5860 6028 6049 6108
Size: 3 168 21 59
Fragments arranged by size:
822 612 548 423 321 321 238 204
181 180 168 168 168 165 120 114
102 99 96 81 81 81 81 81
78 72 63 60 60 59 51 42
30 27 21 21 18 9 3 3
3 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 71 100 604 945 2488 2501 3422 3426
Size: 71 29 504 341 1543 13 921 4
Cuts at: 3426 3494 4118 4286 4388 5291 5590 6108
Size: 68 624 168 102 903 299 518
Fragments arranged by size:
1543 921 903 624 518 504 341 299
168 102 71 68 29 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 570 939 1196 1324 2037 2785 2903
Size: 349 221 369 257 128 713 748 118
Cuts at: 2903 3003 3775 3893 3985 4108 4900 6108
Size: 100 772 118 92 123 792 1208
Fragments arranged by size:
1208 792 772 748 713 369 349 257
221 128 123 118 118 100 92
BsiEI CG_ry'CG
Cuts at: 0 1654 1777 4847 5871 5915 6108
Size: 1654 123 3070 1024 44 193
Fragments arranged by size:
3070 1654 1024 193 123 44
BsiHKAI G_wGCw'C
Cuts at: 0 1912 2807 3367 3677 4507 4812 5280 5741
Size: 1912 895 560 310 830 305 468 461
Cuts at: 5741 6108
Size: 367
Fragments arranged by size:
1912 895 830 560 468 461 367 310
305
BsmBI CGTCTCn'nnnn_
Cuts at: 0 166 665 1057 2550 2890 6108
Size: 166 499 392 1493 340 3218
Fragments arranged by size:
3218 1493 499 392 340 166
Bsp1286I G_dGCh'C
Cuts at: 0 26 55 62 101 130 992 1912 2748
Size: 26 29 7 39 29 862 920 836
Cuts at: 2748 2807 3367 3423 3489 3495 3677 3996 4029
Size: 59 560 56 66 6 182 319 33
Cuts at: 4029 4332 4507 4629 4647 4698 4812 5280 5598
Size: 303 175 122 18 51 114 468 318
Cuts at: 5598 5604 5741 5862 6108
Size: 6 137 121 246
Fragments arranged by size:
920 862 836 560 468 319 318 303
246 182 175 137 122 121 114 66
59 56 51 39 33 29 29 26
18 7 6 6
BspEI T'CCGG_A
Cuts at: 0 2123 6108
Size: 2123 3985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 349 884 1631 1793 2700 3140 4919
Size: 129 220 535 747 162 907 440 1779
Cuts at: 4919 5457 6108
Size: 538 651
Fragments arranged by size:
1779 907 747 651 538 535 440 220
162 129
BsrBI CCG'CTC
Cuts at: 0 254 381 729 1047 1650 3174 4000 4431
Size: 254 127 348 318 603 1524 826 431
Cuts at: 4431 4590 4683 4986 4998 5528 5757 6108
Size: 159 93 303 12 530 229 351
Fragments arranged by size:
1524 826 603 530 431 351 348 318
303 254 229 159 127 93 12
BsrDI GCAATG_nn'
Cuts at: 0 777 1154 5618 6108
Size: 777 377 4464 490
Fragments arranged by size:
4464 777 490 377
BsrFI r'CCGG_y
Cuts at: 0 1004 1653 1916 4442 5080 5866 5951 6108
Size: 1004 649 263 2526 638 786 85 157
Fragments arranged by size:
2526 1004 786 649 638 263 157 85
BssHII G'CGCG_C
Cuts at: 0 2838 6108
Size: 2838 3270
BssSI C'ACGA_G
Cuts at: 0 163 502 1099 2221 3742 3995 6108
Size: 163 339 597 1122 1521 253 2113
Fragments arranged by size:
2113 1521 1122 597 339 253 163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2763 3078 3963 6108
Size: 2763 315 885 2145
Fragments arranged by size:
2763 2145 885 315
BstEII G'GTnAC_C
Cuts at: 0 2529 5332 6108
Size: 2529 2803 776
Fragments arranged by size:
2803 2529 776
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4479 6108
Size: 4479 1629
BstYI r'GATC_y
Cuts at: 0 284 1472 1931 2002 2360 2474 5183 5552
Size: 284 1188 459 71 358 114 2709 369
Cuts at: 5552 6108
Size: 556
Fragments arranged by size:
2709 1188 556 459 369 358 284 114
71
Bsu36I CC'TnA_GG
Cuts at: 0 2620 6080 6108
Size: 2620 3460 28
Fragments arranged by size:
3460 2620 28
BtgI C'CryG_G
Cuts at: 0 93 274 2248 3774 4193 4415 4472 5018
Size: 93 181 1974 1526 419 222 57 546
Cuts at: 5018 6108
Size: 1090
Fragments arranged by size:
1974 1526 1090 546 419 222 181 93
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1443 6108
Size: 1443 4665
BtsI GCAGTG_nn'
Cuts at: 0 533 2677 2701 3758 4580 4717 6108
Size: 533 2144 24 1057 822 137 1391
Fragments arranged by size:
2144 1391 1057 822 533 137 24
DraIII CAC_nnn'GTG
Cuts at: 0 507 2098 6108
Size: 507 1591 4010
Fragments arranged by size:
4010 1591 507
DrdI GACnn_nn'nnGTC
Cuts at: 0 1071 2527 2542 6108
Size: 1071 1456 15 3566
Fragments arranged by size:
3566 1456 1071 15
EaeI y'GGCC_r
Cuts at: 0 329 1038 1290 1352 1651 1655 1755 1774
Size: 329 709 252 62 299 4 100 19
Cuts at: 1774 2100 2168 3197 3461 3533 3569 3777 4844
Size: 326 68 1029 264 72 36 208 1067
Cuts at: 4844 4955 5015 5082 5261 5324 5868 5912 6108
Size: 111 60 67 179 63 544 44 196
Fragments arranged by size:
1067 1029 709 544 329 326 299 264
252 208 196 179 111 100 72 68
67 63 62 60 44 36 19 4
EagI C'GGCC_G
Cuts at: 0 1651 1774 4844 5868 5912 6108
Size: 1651 123 3070 1024 44 196
Fragments arranged by size:
3070 1651 1024 196 123 44
EarI CTCTTCn'nnn_
Cuts at: 0 1060 1159 1381 1543 2221 2338 2504 2825
Size: 1060 99 222 162 678 117 166 321
Cuts at: 2825 3004 3119 4931 5396 6108
Size: 179 115 1812 465 712
Fragments arranged by size:
1812 1060 712 678 465 321 222 179
166 162 117 115 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 45 247 262 889 2024 3598 3814 4959
Size: 45 202 15 627 1135 1574 216 1145
Cuts at: 4959 6108
Size: 1149
Fragments arranged by size:
1574 1149 1145 1135 627 216 202 45
15
EcoICRI GAG'CTC
Cuts at: 0 2805 3365 3675 6108
Size: 2805 560 310 2433
Fragments arranged by size:
2805 2433 560 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 620 724 745 901 977 1090 1126
Size: 611 9 104 21 156 76 113 36
Cuts at: 1126 1363 1582 1788 2263 2308 2545 2791 2953
Size: 237 219 206 475 45 237 246 162
Cuts at: 2953 2974 3106 3352 3361 3574 3637 3658 3730
Size: 21 132 246 9 213 63 21 72
Cuts at: 3730 3742 3829 3880 3964 4060 4249 4354 4447
Size: 12 87 51 84 96 189 105 93
Cuts at: 4447 4501 4531 4669 4699 4732 4846 4888 5059
Size: 54 30 138 30 33 114 42 171
Cuts at: 5059 5071 5080 5248 5377 5395 5428 5725 5809
Size: 12 9 168 129 18 33 297 84
Cuts at: 5809 5839 5851 6108
Size: 30 12 257
Fragments arranged by size:
611 475 297 257 246 246 237 237
219 213 206 189 171 168 162 156
138 132 129 114 113 105 104 96
93 87 84 84 76 72 63 54
51 45 42 36 33 33 30 30
30 21 21 21 18 12 12 12
9 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 1354 2335 3304 3514 3535 3607 5341
Size: 349 1005 981 969 210 21 72 1734
Cuts at: 5341 5578 6108
Size: 237 530
Fragments arranged by size:
1734 1005 981 969 530 349 237 210
72 21
EcoO109I rG'GnC_Cy
Cuts at: 0 539 1083 1347 2283 2493 2585 3129 3485
Size: 539 544 264 936 210 92 544 356
Cuts at: 3485 3549 3600 3783 4178 4380 4626 4695 5223
Size: 64 51 183 395 202 246 69 528
Cuts at: 5223 5594 6086 6108
Size: 371 492 22
Fragments arranged by size:
936 544 544 539 528 492 395 371
356 264 246 210 202 183 92 69
64 51 22
EcoRI G'AATT_C
Cuts at: 0 3153 6108
Size: 3153 2955
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2496 6108
Size: 2496 3612
FseI GG_CCGG'CC
Cuts at: 0 1657 5870 6108
Size: 1657 4213 238
Fragments arranged by size:
4213 1657 238
FspI TGC'GCA
Cuts at: 0 5707 6108
Size: 5707 401
HaeII r_GCGC'y
Cuts at: 0 40 208 501 860 956 1374 1436 2434
Size: 40 168 293 359 96 418 62 998
Cuts at: 2434 2601 2618 2789 3597 3625 3774 3939 4060
Size: 167 17 171 808 28 149 165 121
Cuts at: 4060 4311 4359 4395 5217 5385 6108
Size: 251 48 36 822 168 723
Fragments arranged by size:
998 822 808 723 418 359 293 251
171 168 168 167 165 149 121 96
62 48 40 36 28 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 784 816 2345 2377 3193 3225
Size: 559 32 193 32 1529 32 816 32
Cuts at: 3225 3541 3573 3664 3696 3897 3929 4033 4057
Size: 316 32 91 32 201 32 104 24
Cuts at: 4057 4065 4089 4404 4436 5669 5701 5836 5868
Size: 8 24 315 32 1233 32 135 32
Cuts at: 5868 6108
Size: 240
Fragments arranged by size:
1529 1233 816 559 316 315 240 201
193 135 104 91 32 32 32 32
32 32 32 32 32 32 24 24
8
HincII GTy'rAC
Cuts at: 0 1833 6108
Size: 1833 4275
KasI G'GCGC_C
Cuts at: 0 36 856 1432 3935 4307 4355 5381 6108
Size: 36 820 576 2503 372 48 1026 727
Fragments arranged by size:
2503 1026 820 727 576 372 48 36
MfeI C'AATT_G
Cuts at: 0 2857 6108
Size: 2857 3251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5375 6030 6053 6108
Size: 5375 655 23 55
Fragments arranged by size:
5375 655 55 23
MscI TGG'CCA
Cuts at: 0 1354 2170 3463 3571 4957 5326 6108
Size: 1354 816 1293 108 1386 369 782
Fragments arranged by size:
1386 1354 1293 816 782 369 108
MslI CAynn'nnrTG
Cuts at: 0 19 1114 1555 4904 6108
Size: 19 1095 441 3349 1204
Fragments arranged by size:
3349 1204 1095 441 19
MspA1I CmG'CkG
Cuts at: 0 95 276 735 942 1210 1382 1482 1773
Size: 95 181 459 207 268 172 100 291
Cuts at: 1773 2204 2250 2505 2560 2634 2826 3084 3220
Size: 431 46 255 55 74 192 258 136
Cuts at: 3220 3402 3426 3498 3807 3930 3954 4122 4227
Size: 182 24 72 309 123 24 168 105
Cuts at: 4227 4243 4491 4561 4843 4953 4965 5373 5497
Size: 16 248 70 282 110 12 408 124
Cuts at: 5497 5541 5787 5817 5829 6108
Size: 44 246 30 12 279
Fragments arranged by size:
459 431 408 309 291 282 279 268
258 255 248 246 207 192 182 181
172 168 136 124 123 110 105 100
95 74 72 70 55 46 44 30
24 24 16 12 12
NaeI GCC'GGC
Cuts at: 0 1655 4444 5082 5868 6108
Size: 1655 2789 638 786 240
Fragments arranged by size:
2789 1655 786 638 240
NarI GG'CG_CC
Cuts at: 0 37 857 1433 3936 4308 4356 5382 6108
Size: 37 820 576 2503 372 48 1026 726
Fragments arranged by size:
2503 1026 820 726 576 372 48 37
NcoI C'CATG_G
Cuts at: 0 4193 4472 6108
Size: 4193 279 1636
Fragments arranged by size:
4193 1636 279
NgoMIV G'CCGG_C
Cuts at: 0 1653 4442 5080 5866 6108
Size: 1653 2789 638 786 242
Fragments arranged by size:
2789 1653 786 638 242
NspI r_CATG'y
Cuts at: 0 555 6108
Size: 555 5553
PasI CC'CwG_GG
Cuts at: 0 3786 6108
Size: 3786 2322
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1850 2098 2433 3330 3786 4395 4542
Size: 945 905 248 335 897 456 609 147
Cuts at: 4542 6108
Size: 1566
Fragments arranged by size:
1566 945 905 897 609 456 335 248
147
PfoI T'CCnGG_A
Cuts at: 0 374 1058 1451 2285 2336 3140 5555 5777
Size: 374 684 393 834 51 804 2415 222
Cuts at: 5777 6108
Size: 331
Fragments arranged by size:
2415 834 804 684 393 374 331 222
51
PmlI CAC'GTG
Cuts at: 0 519 651 4669 6108
Size: 519 132 4018 1439
Fragments arranged by size:
4018 1439 519 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1684 1716 6108
Size: 1684 32 4392
Fragments arranged by size:
4392 1684 32
PpuMI rG'GwC_Cy
Cuts at: 0 1083 2283 2493 3129 3549 3600 4178 6086
Size: 1083 1200 210 636 420 51 578 1908
Cuts at: 6086 6108
Size: 22
Fragments arranged by size:
1908 1200 1083 636 578 420 210 51
22
PspOMI G'GGCC_C
Cuts at: 0 51 97 2744 3419 3485 3491 4625 4694
Size: 51 46 2647 675 66 6 1134 69
Cuts at: 4694 5594 6108
Size: 900 514
Fragments arranged by size:
2647 1134 900 675 514 69 66 51
46 6
PspXI vC'TCGA_Gb
Cuts at: 0 985 6108
Size: 985 5123
PstI C_TGCA'G
Cuts at: 0 560 1532 1628 2010 2711 2738 2759 3083
Size: 560 972 96 382 701 27 21 324
Cuts at: 3083 3377 3452 3905 3959 4022 4148 4274 4451
Size: 294 75 453 54 63 126 126 177
Cuts at: 4451 4916 5252 5468 5885 6108
Size: 465 336 216 417 223
Fragments arranged by size:
972 701 560 465 453 417 382 336
324 294 223 216 177 126 126 96
75 63 54 27 21
PvuII CAG'CTG
Cuts at: 0 735 942 1210 1482 2505 2560 2826 3084
Size: 735 207 268 272 1023 55 266 258
Cuts at: 3084 3220 3426 3498 3807 3954 4122 4227 4243
Size: 136 206 72 309 147 168 105 16
Cuts at: 4243 4561 4953 4965 5373 5497 5541 5787 5817
Size: 318 392 12 408 124 44 246 30
Cuts at: 5817 5829 6108
Size: 12 279
Fragments arranged by size:
1023 735 408 392 318 309 279 272
268 266 258 246 207 206 168 147
136 124 105 72 55 44 30 16
12 12
RsrII CG'GwC_CG
Cuts at: 0 5271 6108
Size: 5271 837
SacI G_AGCT'C
Cuts at: 0 2807 3367 3677 6108
Size: 2807 560 310 2431
Fragments arranged by size:
2807 2431 560 310
SacII CC_GC'GG
Cuts at: 0 96 277 2251 6108
Size: 96 181 1974 3857
Fragments arranged by size:
3857 1974 181 96
SalI G'TCGA_C
Cuts at: 0 1831 6108
Size: 1831 4277
SanDI GG'GwC_CC
Cuts at: 0 4178 6086 6108
Size: 4178 1908 22
Fragments arranged by size:
4178 1908 22
SapI GCTCTTCn'nnn_
Cuts at: 0 1543 5396 6108
Size: 1543 3853 712
Fragments arranged by size:
3853 1543 712
SbfI CC_TGCA'GG
Cuts at: 0 4916 5468 6108
Size: 4916 552 640
Fragments arranged by size:
4916 640 552
SexAI A'CCwGG_T
Cuts at: 0 2094 6108
Size: 2094 4014
SfcI C'TryA_G
Cuts at: 0 556 1528 1624 2006 2707 2734 2755 2773
Size: 556 972 96 382 701 27 21 18
Cuts at: 2773 3016 3079 3373 3448 3901 3955 4018 4144
Size: 243 63 294 75 453 54 63 126
Cuts at: 4144 4270 4447 4912 5248 5464 5881 6108
Size: 126 177 465 336 216 417 227
Fragments arranged by size:
972 701 556 465 453 417 382 336
294 243 227 216 177 126 126 96
75 63 63 54 27 21 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3862 6108
Size: 3862 2246
SfoI GGC'GCC
Cuts at: 0 38 858 1434 3937 4309 4357 5383 6108
Size: 38 820 576 2503 372 48 1026 725
Fragments arranged by size:
2503 1026 820 725 576 372 48 38
SmaI CCC'GGG
Cuts at: 0 27 2566 3490 4183 4369 5176 5857 6108
Size: 27 2539 924 693 186 807 681 251
Fragments arranged by size:
2539 924 807 693 681 251 186 27
SmlI C'TyrA_G
Cuts at: 0 985 2653 3049 3521 4888 5218 5629 6108
Size: 985 1668 396 472 1367 330 411 479
Fragments arranged by size:
1668 1367 985 479 472 411 396 330
SphI G_CATG'C
Cuts at: 0 555 6108
Size: 555 5553
SrfI GCCC'GGGC
Cuts at: 0 27 3490 6108
Size: 27 3463 2618
Fragments arranged by size:
3463 2618 27
StuI AGG'CCT
Cuts at: 0 355 763 2410 6108
Size: 355 408 1647 3698
Fragments arranged by size:
3698 1647 408 355
StyI C'CwwG_G
Cuts at: 0 908 1355 1409 1428 1974 2384 2690 3578
Size: 908 447 54 19 546 410 306 888
Cuts at: 3578 4172 4193 4472 5516 6108
Size: 594 21 279 1044 592
Fragments arranged by size:
1044 908 888 594 592 546 447 410
306 279 54 21 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 159 348 547 2385 5566 6108
Size: 159 189 199 1838 3181 542
Fragments arranged by size:
3181 1838 542 199 189 159
TatI w'GTAC_w
Cuts at: 0 4563 6108
Size: 4563 1545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1229 1261 1678 1710 2965 2997 5687 5719
Size: 1229 32 417 32 1255 32 2690 32
Cuts at: 5719 6108
Size: 389
Fragments arranged by size:
2690 1255 1229 417 389 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 15 1885 6108
Size: 15 1870 4223
Fragments arranged by size:
4223 1870 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1122 1557 2391 4164 4476 4863 6108
Size: 1122 435 834 1773 312 387 1245
Fragments arranged by size:
1773 1245 1122 834 435 387 312
XhoI C'TCGA_G
Cuts at: 0 985 6108
Size: 985 5123
XmaI C'CCGG_G
Cuts at: 0 25 2564 3488 4181 4367 5174 5855 6108
Size: 25 2539 924 693 186 807 681 253
Fragments arranged by size:
2539 924 807 693 681 253 186 25
XmnI GAAnn'nnTTC
Cuts at: 0 1489 6108
Size: 1489 4619
ZraI GAC'GTC
Cuts at: 0 4740 6108
Size: 4740 1368
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfcI
SfiI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AleI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BclI BfrBI BmtI BsaBI
BsiWI BsmI BspHI BsrGI BstBI BstZ17I ClaI CspCI
DraI EcoRV FspAI HindIII HpaI KpnI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PshAI
PsiI PsrI PvuI ScaI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI