Restriction map for
pF1KA2021
(Linear) (Six-base) MAPSORT of: KIAA2021.seq Check: 8592 from: 1 to: 1908
KIAA2021.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 587 1908
Size: 587 1321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 716 754 814 1908
Size: 616 100 38 60 1094
Fragments arranged by size:
1094 616 100 60 38
AfeI AGC'GCT
Cuts at: 0 1111 1908
Size: 1111 797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 340 1705 1877 1908
Size: 340 1365 172 31
Fragments arranged by size:
1365 340 172 31
AlwNI CAG_nnn'CTG
Cuts at: 0 336 777 836 1005 1074 1396 1476 1542
Size: 336 441 59 169 69 322 80 66
Cuts at: 1542 1770 1908
Size: 228 138
Fragments arranged by size:
441 336 322 228 169 138 80 69
66 59
ApaI G_GGCC'C
Cuts at: 0 1728 1908
Size: 1728 180
ApaLI G'TGCA_C
Cuts at: 0 256 1908
Size: 256 1652
AvaI C'yCGr_G
Cuts at: 0 226 1908
Size: 226 1682
BanI G'GyrC_C
Cuts at: 0 35 86 1519 1908
Size: 35 51 1433 389
Fragments arranged by size:
1433 389 51 35
BanII G_rGCy'C
Cuts at: 0 175 784 1022 1171 1304 1728 1908
Size: 175 609 238 149 133 424 180
Fragments arranged by size:
609 424 238 180 175 149 133
BbeI G_GCGC'C
Cuts at: 0 39 90 1908
Size: 39 51 1818
Fragments arranged by size:
1818 51 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 712 832 1908
Size: 712 120 1076
Fragments arranged by size:
1076 712 120
BbvCI CC'TCA_GC
Cuts at: 0 563 1908
Size: 563 1345
BglI GCCn_nnn'nGGC
Cuts at: 0 1723 1908
Size: 1723 185
BglII A'GATC_T
Cuts at: 0 246 824 1908
Size: 246 578 1084
Fragments arranged by size:
1084 578 246
BlpI GC'TnA_GC
Cuts at: 0 1165 1745 1908
Size: 1165 580 163
Fragments arranged by size:
1165 580 163
Bme1580I G_kGCm'C
Cuts at: 0 260 1728 1908
Size: 260 1468 180
Fragments arranged by size:
1468 260 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 242 873 1908
Size: 242 631 1035
Fragments arranged by size:
1035 631 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 556 676 813 1015 1453 1725 1908
Size: 134 422 120 137 202 438 272 183
Fragments arranged by size:
438 422 272 202 183 137 134 120
Bpu10I CC'TnA_GC
Cuts at: 0 169 563 716 778 1136 1908
Size: 169 394 153 62 358 772
Fragments arranged by size:
772 394 358 169 153 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1740 1908
Size: 1740 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 523 1463 1772 1908
Size: 523 940 309 136
Fragments arranged by size:
940 523 309 136
BsaHI Gr'CG_yC
Cuts at: 0 36 87 1908
Size: 36 51 1821
Fragments arranged by size:
1821 51 36
BsaWI w'CCGG_w
Cuts at: 0 126 1908
Size: 126 1782
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1908
Size: 766 30 1112
Fragments arranged by size:
1112 766 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 502 505 508 511 514 517 553
Size: 499 3 3 3 3 3 3 36
Cuts at: 553 774 1060 1174 1393 1396 1908
Size: 221 286 114 219 3 512
Fragments arranged by size:
512 499 286 221 219 114 36 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 45 468 711 1908
Size: 45 423 243 1197
Fragments arranged by size:
1197 423 243 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1316 1465 1908
Size: 1064 252 149 443
Fragments arranged by size:
1064 443 252 149
BsiHKAI G_wGCw'C
Cuts at: 0 260 784 1052 1406 1580 1908
Size: 260 524 268 354 174 328
Fragments arranged by size:
524 354 328 268 260 174
BsmI GAATG_Cn'
Cuts at: 0 1285 1908
Size: 1285 623
Bsp1286I G_dGCh'C
Cuts at: 0 175 260 784 1022 1052 1171 1304 1406
Size: 175 85 524 238 30 119 133 102
Cuts at: 1406 1580 1728 1908
Size: 174 148 180
Fragments arranged by size:
524 238 180 175 174 148 133 119
102 85 30
BspEI T'CCGG_A
Cuts at: 0 126 1908
Size: 126 1782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 990 1908
Size: 990 918
BsrDI GCAATG_nn'
Cuts at: 0 800 906 1088 1214 1421 1908
Size: 800 106 182 126 207 487
Fragments arranged by size:
800 487 207 182 126 106
BsrFI r'CCGG_y
Cuts at: 0 38 144 1908
Size: 38 106 1764
Fragments arranged by size:
1764 106 38
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1005 1396 1479 1908
Size: 1005 391 83 429
Fragments arranged by size:
1005 429 391 83
BstBI TT'CG_AA
Cuts at: 0 1509 1908
Size: 1509 399
BstXI CCAn_nnnn'nTGG
Cuts at: 0 993 1347 1434 1908
Size: 993 354 87 474
Fragments arranged by size:
993 474 354 87
BstYI r'GATC_y
Cuts at: 0 246 824 1908
Size: 246 578 1084
Fragments arranged by size:
1084 578 246
BtgI C'CryG_G
Cuts at: 0 524 678 1677 1908
Size: 524 154 999 231
Fragments arranged by size:
999 524 231 154
BtsI GCAGTG_nn'
Cuts at: 0 324 812 1397 1908
Size: 324 488 585 511
Fragments arranged by size:
585 511 488 324
DraIII CAC_nnn'GTG
Cuts at: 0 30 1410 1797 1908
Size: 30 1380 387 111
Fragments arranged by size:
1380 387 111 30
EaeI y'GGCC_r
Cuts at: 0 118 1418 1788 1868 1908
Size: 118 1300 370 80 40
Fragments arranged by size:
1300 370 118 80 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 1908
Size: 184 1724
EcoICRI GAG'CTC
Cuts at: 0 782 1908
Size: 782 1126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 556 616 676 716 754 813 814
Size: 134 422 60 60 40 38 59 1
Cuts at: 814 1015 1453 1725 1908
Size: 201 438 272 183
Fragments arranged by size:
438 422 272 201 183 134 60 60
59 40 38 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 1042 1908
Size: 997 45 866
Fragments arranged by size:
997 866 45
EcoO109I rG'GnC_Cy
Cuts at: 0 288 674 774 1002 1229 1247 1263 1724
Size: 288 386 100 228 227 18 16 461
Cuts at: 1724 1908
Size: 184
Fragments arranged by size:
461 386 288 228 227 184 100 18
16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1799 1908
Size: 1799 109
FspI TGC'GCA
Cuts at: 0 400 1908
Size: 400 1508
HaeII r_GCGC'y
Cuts at: 0 39 90 138 205 1113 1908
Size: 39 51 48 67 908 795
Fragments arranged by size:
908 795 67 51 48 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 410 442 699 731 766 798
Size: 121 32 257 32 257 32 35 32
Cuts at: 798 811 843 1129 1161 1291 1323 1908
Size: 13 32 286 32 130 32 585
Fragments arranged by size:
585 286 257 257 130 121 35 32
32 32 32 32 32 32 13
KasI G'GCGC_C
Cuts at: 0 35 86 1908
Size: 35 51 1822
Fragments arranged by size:
1822 51 35
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 1259 1908
Size: 995 264 649
Fragments arranged by size:
995 649 264
MscI TGG'CCA
Cuts at: 0 1870 1908
Size: 1870 38
MslI CAynn'nnrTG
Cuts at: 0 805 1345 1908
Size: 805 540 563
Fragments arranged by size:
805 563 540
MspA1I CmG'CkG
Cuts at: 0 49 562 733 1065 1564 1908
Size: 49 513 171 332 499 344
Fragments arranged by size:
513 499 344 332 171 49
NaeI GCC'GGC
Cuts at: 0 146 1908
Size: 146 1762
NarI GG'CG_CC
Cuts at: 0 36 87 1908
Size: 36 51 1821
Fragments arranged by size:
1821 51 36
NcoI C'CATG_G
Cuts at: 0 1677 1908
Size: 1677 231
NgoMIV G'CCGG_C
Cuts at: 0 144 1908
Size: 144 1764
PasI CC'CwG_GG
Cuts at: 0 1194 1204 1908
Size: 1194 10 704
Fragments arranged by size:
1194 704 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 52 1797 1908
Size: 52 1745 111
Fragments arranged by size:
1745 111 52
PfoI T'CCnGG_A
Cuts at: 0 992 1908
Size: 992 916
PpuMI rG'GwC_Cy
Cuts at: 0 774 1002 1263 1908
Size: 774 228 261 645
Fragments arranged by size:
774 645 261 228
PspOMI G'GGCC_C
Cuts at: 0 1724 1908
Size: 1724 184
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 915 947 1908
Size: 915 32 961
Fragments arranged by size:
961 915 32
PstI C_TGCA'G
Cuts at: 0 332 483 1070 1401 1637 1908
Size: 332 151 587 331 236 271
Fragments arranged by size:
587 332 331 271 236 151
PvuII CAG'CTG
Cuts at: 0 49 733 1065 1908
Size: 49 684 332 843
Fragments arranged by size:
843 684 332 49
SacI G_AGCT'C
Cuts at: 0 784 1908
Size: 784 1124
ScaI AGT'ACT
Cuts at: 0 924 1908
Size: 924 984
SexAI A'CCwGG_T
Cuts at: 0 1406 1908
Size: 1406 502
SfcI C'TryA_G
Cuts at: 0 328 451 479 1066 1267 1397 1633 1668
Size: 328 123 28 587 201 130 236 35
Cuts at: 1668 1908
Size: 240
Fragments arranged by size:
587 328 240 236 201 130 123 35
28
SfoI GGC'GCC
Cuts at: 0 37 88 1908
Size: 37 51 1820
Fragments arranged by size:
1820 51 37
SmlI C'TyrA_G
Cuts at: 0 1719 1908
Size: 1719 189
StyI C'CwwG_G
Cuts at: 0 74 1259 1433 1452 1677 1908
Size: 74 1185 174 19 225 231
Fragments arranged by size:
1185 231 225 174 74 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1217 1507 1908
Size: 1217 290 401
Fragments arranged by size:
1217 401 290
TatI w'GTAC_w
Cuts at: 0 922 1361 1503 1908
Size: 922 439 142 405
Fragments arranged by size:
922 439 405 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 303 335 1640 1672 1908
Size: 303 32 1305 32 236
Fragments arranged by size:
1305 303 236 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 531 1561 1908
Size: 531 1030 347
Fragments arranged by size:
1030 531 347
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI
BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI
PpuMI PspOMI PsrI PstI PvuII SacI ScaI SexAI
SfcI SfoI SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BmgBI BmtI BplI
BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BsrGI
BssHII BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EarI EcoRI EcoRV FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI