Restriction map for
pF1KA2015
(Linear) (Six-base) MAPSORT of: KIAA2015.seq Check: 1054 from: 1 to: 2976
KIAA2015.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1271 1807 2027 2267 2976
Size: 869 402 536 220 240 709
Fragments arranged by size:
869 709 536 402 240 220
AfeI AGC'GCT
Cuts at: 0 154 2976
Size: 154 2822
AflII C'TTAA_G
Cuts at: 0 2413 2976
Size: 2413 563
AflIII A'CryG_T
Cuts at: 0 689 2976
Size: 689 2287
AlwNI CAG_nnn'CTG
Cuts at: 0 1736 2976
Size: 1736 1240
ApoI r'AATT_y
Cuts at: 0 525 550 752 831 978 1203 1867 1997
Size: 525 25 202 79 147 225 664 130
Cuts at: 1997 2023 2626 2677 2909 2976
Size: 26 603 51 232 67
Fragments arranged by size:
664 603 525 232 225 202 147 130
79 67 51 26 25
AseI AT'TA_AT
Cuts at: 0 1721 1786 1949 2044 2734 2976
Size: 1721 65 163 95 690 242
Fragments arranged by size:
1721 690 242 163 95 65
AvaI C'yCGr_G
Cuts at: 0 1436 2976
Size: 1436 1540
BanI G'GyrC_C
Cuts at: 0 370 2976
Size: 370 2606
BanII G_rGCy'C
Cuts at: 0 52 2976
Size: 52 2924
BbeI G_GCGC'C
Cuts at: 0 374 2976
Size: 374 2602
BbsI GAAGACnn'nnnn_
Cuts at: 0 2273 2976
Size: 2273 703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 697 731 2976
Size: 697 34 2245
Fragments arranged by size:
2245 697 34
BclI T'GATC_A
Cuts at: 0 2762 2976
Size: 2762 214
BfrBI ATG'CAT
Cuts at: 0 1572 2158 2976
Size: 1572 586 818
Fragments arranged by size:
1572 818 586
BglII A'GATC_T
Cuts at: 0 1758 2976
Size: 1758 1218
Bme1580I G_kGCm'C
Cuts at: 0 231 2976
Size: 231 2745
BmrI ACTGGGnnnn_n'
Cuts at: 0 105 2976
Size: 105 2871
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1504 2976
Size: 1504 1472
Bpu10I CC'TnA_GC
Cuts at: 0 46 2976
Size: 46 2930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1762 2392 2976
Size: 668 1094 630 584
Fragments arranged by size:
1094 668 630 584
BsaAI yAC'GTr
Cuts at: 0 673 2976
Size: 673 2303
BsaBI GATnn'nnATC
Cuts at: 0 283 2976
Size: 283 2693
BsaHI Gr'CG_yC
Cuts at: 0 27 137 185 371 2976
Size: 27 110 48 186 2605
Fragments arranged by size:
2605 186 110 48 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1642 2976
Size: 1642 1334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 2512 2976
Size: 247 2265 464
Fragments arranged by size:
2265 464 247
BsiHKAI G_wGCw'C
Cuts at: 0 52 2976
Size: 52 2924
BsmI GAATG_Cn'
Cuts at: 0 1197 2976
Size: 1197 1779
BsmBI CGTCTCn'nnnn_
Cuts at: 0 18 2976
Size: 18 2958
Bsp1286I G_dGCh'C
Cuts at: 0 52 231 2976
Size: 52 179 2745
Fragments arranged by size:
2745 179 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 2976
Size: 158 2818
BsrDI GCAATG_nn'
Cuts at: 0 998 2976
Size: 998 1978
BsrGI T'GTAC_A
Cuts at: 0 324 1518 2349 2976
Size: 324 1194 831 627
Fragments arranged by size:
1194 831 627 324
BstBI TT'CG_AA
Cuts at: 0 792 2976
Size: 792 2184
BstYI r'GATC_y
Cuts at: 0 110 1758 2976
Size: 110 1648 1218
Fragments arranged by size:
1648 1218 110
Bsu36I CC'TnA_GG
Cuts at: 0 1531 2976
Size: 1531 1445
BtgI C'CryG_G
Cuts at: 0 53 140 231 2184 2976
Size: 53 87 91 1953 792
Fragments arranged by size:
1953 792 91 87 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 982 2976
Size: 982 1994
BtsI GCAGTG_nn'
Cuts at: 0 404 1280 2495 2708 2765 2976
Size: 404 876 1215 213 57 211
Fragments arranged by size:
1215 876 404 213 211 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1130 1165 2976
Size: 1130 35 1811
Fragments arranged by size:
1811 1130 35
DraI TTT'AAA
Cuts at: 0 1808 2976
Size: 1808 1168
EaeI y'GGCC_r
Cuts at: 0 234 696 2261 2300 2900 2976
Size: 234 462 1565 39 600 76
Fragments arranged by size:
1565 600 462 234 76 39
EarI CTCTTCn'nnn_
Cuts at: 0 16 332 684 2099 2648 2822 2976
Size: 16 316 352 1415 549 174 154
Fragments arranged by size:
1415 549 352 316 174 154 16
EcoICRI GAG'CTC
Cuts at: 0 50 2976
Size: 50 2926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1271 1807 2027 2267 2976
Size: 869 402 536 220 240 709
Fragments arranged by size:
869 709 536 402 240 220
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 2976
Size: 34 2942
EcoRI G'AATT_C
Cuts at: 0 1203 2023 2909 2976
Size: 1203 820 886 67
Fragments arranged by size:
1203 886 820 67
EcoRV GAT'ATC
Cuts at: 0 393 2976
Size: 393 2583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2706 2970 2976
Size: 2706 264 6
Fragments arranged by size:
2706 264 6
HaeII r_GCGC'y
Cuts at: 0 43 156 374 2976
Size: 43 113 218 2602
Fragments arranged by size:
2602 218 113 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 1623 1655 2320 2352 2976
Size: 263 32 1328 32 665 32 624
Fragments arranged by size:
1328 665 624 263 32 32 32
HincII GTy'rAC
Cuts at: 0 588 1642 2976
Size: 588 1054 1334
Fragments arranged by size:
1334 1054 588
KasI G'GCGC_C
Cuts at: 0 370 2976
Size: 370 2606
MfeI C'AATT_G
Cuts at: 0 2178 2976
Size: 2178 798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 2372 2976
Size: 768 1604 604
Fragments arranged by size:
1604 768 604
MscI TGG'CCA
Cuts at: 0 698 2302 2976
Size: 698 1604 674
Fragments arranged by size:
1604 698 674
MslI CAynn'nnrTG
Cuts at: 0 236 1003 1577 2976
Size: 236 767 574 1399
Fragments arranged by size:
1399 767 574 236
MspA1I CmG'CkG
Cuts at: 0 142 1524 2793 2976
Size: 142 1382 1269 183
Fragments arranged by size:
1382 1269 183 142
NarI GG'CG_CC
Cuts at: 0 371 2976
Size: 371 2605
NcoI C'CATG_G
Cuts at: 0 53 231 2184 2976
Size: 53 178 1953 792
Fragments arranged by size:
1953 792 178 53
NdeI CA'TA_TG
Cuts at: 0 542 2423 2976
Size: 542 1881 553
Fragments arranged by size:
1881 553 542
NsiI A_TGCA'T
Cuts at: 0 1574 2160 2976
Size: 1574 586 816
Fragments arranged by size:
1574 816 586
NspI r_CATG'y
Cuts at: 0 583 693 2976
Size: 583 110 2283
Fragments arranged by size:
2283 583 110
PciI A'CATG_T
Cuts at: 0 689 2976
Size: 689 2287
PfoI T'CCnGG_A
Cuts at: 0 173 359 529 2976
Size: 173 186 170 2447
Fragments arranged by size:
2447 186 173 170
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2825 2857 2976
Size: 2825 32 119
Fragments arranged by size:
2825 119 32
PvuII CAG'CTG
Cuts at: 0 1524 2976
Size: 1524 1452
SacI G_AGCT'C
Cuts at: 0 52 2976
Size: 52 2924
SacII CC_GC'GG
Cuts at: 0 143 2976
Size: 143 2833
SapI GCTCTTCn'nnn_
Cuts at: 0 16 2976
Size: 16 2960
SfoI GGC'GCC
Cuts at: 0 372 2976
Size: 372 2604
SmlI C'TyrA_G
Cuts at: 0 683 1741 2371 2413 2976
Size: 683 1058 630 42 563
Fragments arranged by size:
1058 683 630 563 42
SnaBI TAC'GTA
Cuts at: 0 673 2976
Size: 673 2303
SpeI A'CTAG_T
Cuts at: 0 1329 2976
Size: 1329 1647
SspI AAT'ATT
Cuts at: 0 477 1383 2803 2976
Size: 477 906 1420 173
Fragments arranged by size:
1420 906 477 173
StyI C'CwwG_G
Cuts at: 0 53 231 2184 2976
Size: 53 178 1953 792
Fragments arranged by size:
1953 792 178 53
TatI w'GTAC_w
Cuts at: 0 324 621 1295 1518 2349 2976
Size: 324 297 674 223 831 627
Fragments arranged by size:
831 674 627 324 297 223
XbaI T'CTAG_A
Cuts at: 0 1357 1764 2976
Size: 1357 407 1212
Fragments arranged by size:
1357 1212 407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 2976
Size: 357 2619
XmnI GAAnn'nnTTC
Cuts at: 0 10 1760 2318 2976
Size: 10 1750 558 658
Fragments arranged by size:
1750 658 558 10
Enzymes that do cut:
AcuI AfeI AflII AflIII AlwNI ApoI AseI AvaI
BanI BanII BbeI BbsI BcgI BclI BfrBI BglII
Bme1580I BmrI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrGI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI NspI PciI
PfoI PsrI PvuII SacI SacII SapI SfoI SmlI
SnaBI SpeI SspI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbvCI BciVI BglI BlpI BmgBI BmtI BpmI
BsaI BsaWI BsaXI BseYI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I
ClaI DraIII DrdI EagI EciI EcoO109I FseI FspI
FspAI HindIII HpaI KpnI MluI NaeI NgoMIV NheI
NotI NruI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SphI SrfI StuI SwaI TaqII TstI
Tth111I XhoI XmaI ZraI