(Linear) (Six-base) MAPSORT of: KIAA2014.seq Check: 8569 from: 1 to: 3081 KIAA2014.orf With 182 enzymes: SgfI * July 18, 2007 13:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1034 1092 3081 Size: 1034 58 1989 Fragments arranged by size: 1989 1034 58 AatII G_ACGT'C Cuts at: 0 671 3081 Size: 671 2410 Acc65I G'GTAC_C Cuts at: 0 2971 3081 Size: 2971 110 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 655 1749 2133 2308 3081 Size: 655 1094 384 175 773 Fragments arranged by size: 1094 773 655 384 175 AflIII A'CryG_T Cuts at: 0 1130 3081 Size: 1130 1951 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 913 1865 1910 2258 3081 Size: 913 952 45 348 823 Fragments arranged by size: 952 913 823 348 45 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 94 126 3081 Size: 94 32 2955 Fragments arranged by size: 2955 94 32 AlwNI CAG_nnn'CTG Cuts at: 0 2065 2109 2286 2376 3081 Size: 2065 44 177 90 705 Fragments arranged by size: 2065 705 177 90 44 ApaI G_GGCC'C Cuts at: 0 633 1849 3081 Size: 633 1216 1232 Fragments arranged by size: 1232 1216 633 ApaLI G'TGCA_C Cuts at: 0 991 1351 3081 Size: 991 360 1730 Fragments arranged by size: 1730 991 360 ApoI r'AATT_y Cuts at: 0 379 3081 Size: 379 2702 AvaI C'yCGr_G Cuts at: 0 30 3062 3081 Size: 30 3032 19 Fragments arranged by size: 3032 30 19 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 663 696 3081 Size: 663 33 2385 Fragments arranged by size: 2385 663 33 BanI G'GyrC_C Cuts at: 0 2927 2971 3081 Size: 2927 44 110 Fragments arranged by size: 2927 110 44 BanII G_rGCy'C Cuts at: 0 41 92 130 633 1849 3069 3081 Size: 41 51 38 503 1216 1220 12 Fragments arranged by size: 1220 1216 503 51 41 38 12 BbeI G_GCGC'C Cuts at: 0 2931 3081 Size: 2931 150 BbsI GAAGACnn'nnnn_ Cuts at: 0 1710 1755 2267 3081 Size: 1710 45 512 814 Fragments arranged by size: 1710 814 512 45 BbvCI CC'TCA_GC Cuts at: 0 1973 2066 2126 2672 3081 Size: 1973 93 60 546 409 Fragments arranged by size: 1973 546 409 93 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 504 538 3081 Size: 504 34 2543 Fragments arranged by size: 2543 504 34 BciVI GTATCCnnnnn_n' Cuts at: 0 691 2385 3081 Size: 691 1694 696 Fragments arranged by size: 1694 696 691 BglI GCCn_nnn'nGGC Cuts at: 0 553 2668 3081 Size: 553 2115 413 Fragments arranged by size: 2115 553 413 BglII A'GATC_T Cuts at: 0 341 1787 1979 3081 Size: 341 1446 192 1102 Fragments arranged by size: 1446 1102 341 192 BlpI GC'TnA_GC Cuts at: 0 124 3081 Size: 124 2957 Bme1580I G_kGCm'C Cuts at: 0 633 995 1355 1849 3081 Size: 633 362 360 494 1232 Fragments arranged by size: 1232 633 494 362 360 BmgBI CAC'GTC Cuts at: 0 675 2541 3081 Size: 675 1866 540 Fragments arranged by size: 1866 675 540 BmrI ACTGGGnnnn_n' Cuts at: 0 269 432 2326 2740 3044 3081 Size: 269 163 1894 414 304 37 Fragments arranged by size: 1894 414 304 269 163 37 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 60 3081 Size: 60 3021 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 121 128 241 358 531 1204 1453 Size: 31 90 7 113 117 173 673 249 Cuts at: 1453 1505 2131 2353 2542 2635 3053 3081 Size: 52 626 222 189 93 418 28 Fragments arranged by size: 673 626 418 249 222 189 173 117 113 93 90 52 31 28 7 Bpu10I CC'TnA_GC Cuts at: 0 86 1973 2066 2126 2672 2843 3081 Size: 86 1887 93 60 546 171 238 Fragments arranged by size: 1887 546 238 171 93 86 60 BsaI GGTCTCn'nnnn_ Cuts at: 0 1159 3081 Size: 1159 1922 BsaHI Gr'CG_yC Cuts at: 0 668 2257 2660 2928 3081 Size: 668 1589 403 268 153 Fragments arranged by size: 1589 668 403 268 153 BsaWI w'CCGG_w Cuts at: 0 503 602 929 2408 2609 3081 Size: 503 99 327 1479 201 472 Fragments arranged by size: 1479 503 472 327 201 99 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 94 124 1198 1228 2712 2742 3081 Size: 94 30 1074 30 1484 30 339 Fragments arranged by size: 1484 1074 339 94 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 315 854 1072 1210 1219 1399 1552 1990 Size: 315 539 218 138 9 180 153 438 Cuts at: 1990 2128 2689 2830 3081 Size: 138 561 141 251 Fragments arranged by size: 561 539 438 315 251 218 180 153 141 138 138 9 BseYI C'CCAG_C Cuts at: 0 541 1047 1614 2171 2550 2879 3081 Size: 541 506 567 557 379 329 202 Fragments arranged by size: 567 557 541 506 379 329 202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 883 1126 1622 2473 2604 3026 3081 Size: 328 555 243 496 851 131 422 55 Fragments arranged by size: 851 555 496 422 328 243 131 55 BsiHKAI G_wGCw'C Cuts at: 0 130 602 995 1355 3081 Size: 130 472 393 360 1726 Fragments arranged by size: 1726 472 393 360 130 Bsp1286I G_dGCh'C Cuts at: 0 41 92 130 602 633 995 1355 1849 Size: 41 51 38 472 31 362 360 494 Cuts at: 1849 3069 3081 Size: 1220 12 Fragments arranged by size: 1220 494 472 362 360 51 41 38 31 12 BspEI T'CCGG_A Cuts at: 0 929 2609 3081 Size: 929 1680 472 Fragments arranged by size: 1680 929 472 BspHI T'CATG_A Cuts at: 0 701 3081 Size: 701 2380 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 146 506 539 1034 1092 2225 3081 Size: 146 360 33 495 58 1133 856 Fragments arranged by size: 1133 856 495 360 146 58 33 BsrBI CCG'CTC Cuts at: 0 1967 2094 2100 3081 Size: 1967 127 6 981 Fragments arranged by size: 1967 981 127 6 BsrDI GCAATG_nn' Cuts at: 0 2249 3081 Size: 2249 832 BsrFI r'CCGG_y Cuts at: 0 2945 3081 Size: 2945 136 BssHII G'CGCG_C Cuts at: 0 586 3081 Size: 586 2495 BstAPI GCAn_nnn'nTGC Cuts at: 0 1359 2487 3081 Size: 1359 1128 594 Fragments arranged by size: 1359 1128 594 BstBI TT'CG_AA Cuts at: 0 1677 3081 Size: 1677 1404 BstXI CCAn_nnnn'nTGG Cuts at: 0 1753 1938 2859 3081 Size: 1753 185 921 222 Fragments arranged by size: 1753 921 222 185 BstYI r'GATC_y Cuts at: 0 192 341 1787 1803 1979 2379 3081 Size: 192 149 1446 16 176 400 702 Fragments arranged by size: 1446 702 400 192 176 149 16 Bsu36I CC'TnA_GG Cuts at: 0 1535 3081 Size: 1535 1546 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2418 3081 Size: 2418 663 BtsI GCAGTG_nn' Cuts at: 0 2419 2519 3000 3081 Size: 2419 100 481 81 Fragments arranged by size: 2419 481 100 81 DraIII CAC_nnn'GTG Cuts at: 0 999 1350 3081 Size: 999 351 1731 Fragments arranged by size: 1731 999 351 DrdI GACnn_nn'nnGTC Cuts at: 0 673 2602 3081 Size: 673 1929 479 Fragments arranged by size: 1929 673 479 EaeI y'GGCC_r Cuts at: 0 1457 1940 1962 3081 Size: 1457 483 22 1119 Fragments arranged by size: 1457 1119 483 22 EarI CTCTTCn'nnn_ Cuts at: 0 353 1556 2152 3081 Size: 353 1203 596 929 Fragments arranged by size: 1203 929 596 353 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1559 3081 Size: 1559 1522 EcoICRI GAG'CTC Cuts at: 0 128 3081 Size: 128 2953 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 121 128 241 358 531 655 1204 Size: 31 90 7 113 117 173 124 549 Cuts at: 1204 1453 1505 1749 2131 2133 2308 2353 2542 Size: 249 52 244 382 2 175 45 189 Cuts at: 2542 2635 3053 3081 Size: 93 418 28 Fragments arranged by size: 549 418 382 249 244 189 175 173 124 117 113 93 90 52 45 31 28 7 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 513 1186 1954 3081 Size: 513 673 768 1127 Fragments arranged by size: 1127 768 673 513 EcoO109I rG'GnC_Cy Cuts at: 0 27 528 629 630 1428 1452 1468 1542 Size: 27 501 101 1 798 24 16 74 Cuts at: 1542 1797 1845 1846 3073 3081 Size: 255 48 1 1227 8 Fragments arranged by size: 1227 798 501 255 101 74 48 27 24 16 8 1 1 HaeII r_GCGC'y Cuts at: 0 20 548 2931 3081 Size: 20 528 2383 150 Fragments arranged by size: 2383 528 150 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 412 444 1198 1230 2364 2383 2396 2415 Size: 412 32 754 32 1134 19 13 19 Cuts at: 2415 2712 2744 3081 Size: 297 32 337 Fragments arranged by size: 1134 754 412 337 297 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 0 317 1655 2168 3081 Size: 317 1338 513 913 Fragments arranged by size: 1338 913 513 317 HindIII A'AGCT_T Cuts at: 0 1231 3081 Size: 1231 1850 KasI G'GCGC_C Cuts at: 0 2927 3081 Size: 2927 154 KpnI G_GTAC'C Cuts at: 0 2975 3081 Size: 2975 106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1133 1181 1526 1895 2279 3081 Size: 1133 48 345 369 384 802 Fragments arranged by size: 1133 802 384 369 345 48 MscI TGG'CCA Cuts at: 0 1459 1942 3081 Size: 1459 483 1139 Fragments arranged by size: 1459 1139 483 MslI CAynn'nnrTG Cuts at: 0 743 988 3081 Size: 743 245 2093 Fragments arranged by size: 2093 743 245 MspA1I CmG'CkG Cuts at: 0 823 1287 1296 2125 2655 2838 2883 3081 Size: 823 464 9 829 530 183 45 198 Fragments arranged by size: 829 823 530 464 198 183 45 9 NarI GG'CG_CC Cuts at: 0 2928 3081 Size: 2928 153 NdeI CA'TA_TG Cuts at: 0 1329 3081 Size: 1329 1752 NspI r_CATG'y Cuts at: 0 972 1213 1494 3081 Size: 972 241 281 1587 Fragments arranged by size: 1587 972 281 241 PasI CC'CwG_GG Cuts at: 0 619 3081 Size: 619 2462 PflMI CCAn_nnn'nTGG Cuts at: 0 2178 2376 3012 3081 Size: 2178 198 636 69 Fragments arranged by size: 2178 636 198 69 PfoI T'CCnGG_A Cuts at: 0 508 2207 3081 Size: 508 1699 874 Fragments arranged by size: 1699 874 508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 94 126 3081 Size: 94 32 2955 Fragments arranged by size: 2955 94 32 PpuMI rG'GwC_Cy Cuts at: 0 27 528 1542 1797 3081 Size: 27 501 1014 255 1284 Fragments arranged by size: 1284 1014 501 255 27 PshAI GACnn'nnGTC Cuts at: 0 1607 3081 Size: 1607 1474 PspOMI G'GGCC_C Cuts at: 0 629 1845 3081 Size: 629 1216 1236 Fragments arranged by size: 1236 1216 629 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 697 729 3081 Size: 697 32 2352 Fragments arranged by size: 2352 697 32 PstI C_TGCA'G Cuts at: 0 536 1031 1070 1103 1476 1987 2222 2511 Size: 536 495 39 33 373 511 235 289 Cuts at: 2511 3081 Size: 570 Fragments arranged by size: 570 536 511 495 373 289 235 39 33 PvuII CAG'CTG Cuts at: 0 823 1287 2125 2838 3081 Size: 823 464 838 713 243 Fragments arranged by size: 838 823 713 464 243 SacI G_AGCT'C Cuts at: 0 130 3081 Size: 130 2951 SanDI GG'GwC_CC Cuts at: 0 27 3081 Size: 27 3054 SapI GCTCTTCn'nnn_ Cuts at: 0 2152 3081 Size: 2152 929 ScaI AGT'ACT Cuts at: 0 326 3081 Size: 326 2755 SexAI A'CCwGG_T Cuts at: 0 725 1346 3081 Size: 725 621 1735 Fragments arranged by size: 1735 725 621 SfcI C'TryA_G Cuts at: 0 532 1027 1066 1099 1291 1472 1983 2218 Size: 532 495 39 33 192 181 511 235 Cuts at: 2218 2507 3081 Size: 289 574 Fragments arranged by size: 574 532 511 495 289 235 192 181 39 33 SfoI GGC'GCC Cuts at: 0 2929 3081 Size: 2929 152 SmaI CCC'GGG Cuts at: 0 32 3081 Size: 32 3049 SphI G_CATG'C Cuts at: 0 972 1494 3081 Size: 972 522 1587 Fragments arranged by size: 1587 972 522 SspI AAT'ATT Cuts at: 0 948 3081 Size: 948 2133 StuI AGG'CCT Cuts at: 0 404 1390 2680 2996 3081 Size: 404 986 1290 316 85 Fragments arranged by size: 1290 986 404 316 85 StyI C'CwwG_G Cuts at: 0 787 2936 3081 Size: 787 2149 145 Fragments arranged by size: 2149 787 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 356 360 555 3081 Size: 356 4 195 2526 Fragments arranged by size: 2526 356 195 4 TatI w'GTAC_w Cuts at: 0 324 411 3081 Size: 324 87 2670 Fragments arranged by size: 2670 324 87 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2179 3081 Size: 2179 902 XmaI C'CCGG_G Cuts at: 0 30 3081 Size: 30 3051 XmnI GAAnn'nnTTC Cuts at: 0 292 3081 Size: 292 2789 ZraI GAC'GTC Cuts at: 0 669 3081 Size: 669 2412 Enzymes that do cut: AarI AatII Acc65I AcuI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NarI NdeI NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SmaI SphI SspI StuI StyI TaqII TatI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AgeI AhdI AleI AscI AseI AsiSI AvrII BamHI BclI BfrBI BmtI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BsrGI BssSI BstEII BstZ17I BtgI ClaI CspCI DraI EagI EcoRI EcoRV FalI FseI FspI FspAI HpaI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PsiI PspXI PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XhoI