Restriction map for pF1KA2014
 (Linear) (Six-base) MAPSORT of: KIAA2014.seq  Check: 8569  from: 1  to: 3081



KIAA2014.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1034    1092    3081 
   Size:       1034      58    1989

  Fragments arranged by size: 

                1989    1034      58
 
AatII G_ACGT'C

Cuts at:      0     671    3081 
   Size:        671    2410
 
Acc65I G'GTAC_C

Cuts at:      0    2971    3081 
   Size:       2971     110
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655    1749    2133    2308    3081 
   Size:        655    1094     384     175     773

  Fragments arranged by size: 

                1094     773     655     384     175
 
AflIII A'CryG_T

Cuts at:      0    1130    3081 
   Size:       1130    1951
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     913    1865    1910    2258    3081 
   Size:        913     952      45     348     823

  Fragments arranged by size: 

                 952     913     823     348      45
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      94     126    3081 
   Size:         94      32    2955

  Fragments arranged by size: 

                2955      94      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2065    2109    2286    2376    3081 
   Size:       2065      44     177      90     705

  Fragments arranged by size: 

                2065     705     177      90      44
 
ApaI G_GGCC'C

Cuts at:      0     633    1849    3081 
   Size:        633    1216    1232

  Fragments arranged by size: 

                1232    1216     633
 
ApaLI G'TGCA_C

Cuts at:      0     991    1351    3081 
   Size:        991     360    1730

  Fragments arranged by size: 

                1730     991     360
 
ApoI r'AATT_y

Cuts at:      0     379    3081 
   Size:        379    2702
 
AvaI C'yCGr_G

Cuts at:      0      30    3062    3081 
   Size:         30    3032      19

  Fragments arranged by size: 

                3032      30      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     663     696    3081 
   Size:        663      33    2385

  Fragments arranged by size: 

                2385     663      33
 
BanI G'GyrC_C

Cuts at:      0    2927    2971    3081 
   Size:       2927      44     110

  Fragments arranged by size: 

                2927     110      44
 
BanII G_rGCy'C

Cuts at:      0      41      92     130     633    1849    3069    3081 
   Size:         41      51      38     503    1216    1220      12

  Fragments arranged by size: 

                1220    1216     503      51      41      38      12
 
BbeI G_GCGC'C

Cuts at:      0    2931    3081 
   Size:       2931     150
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1710    1755    2267    3081 
   Size:       1710      45     512     814

  Fragments arranged by size: 

                1710     814     512      45
 
BbvCI CC'TCA_GC

Cuts at:      0    1973    2066    2126    2672    3081 
   Size:       1973      93      60     546     409

  Fragments arranged by size: 

                1973     546     409      93      60
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     504     538    3081 
   Size:        504      34    2543

  Fragments arranged by size: 

                2543     504      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     691    2385    3081 
   Size:        691    1694     696

  Fragments arranged by size: 

                1694     696     691
 
BglI GCCn_nnn'nGGC

Cuts at:      0     553    2668    3081 
   Size:        553    2115     413

  Fragments arranged by size: 

                2115     553     413
 
BglII A'GATC_T

Cuts at:      0     341    1787    1979    3081 
   Size:        341    1446     192    1102

  Fragments arranged by size: 

                1446    1102     341     192
 
BlpI GC'TnA_GC

Cuts at:      0     124    3081 
   Size:        124    2957
 
Bme1580I G_kGCm'C

Cuts at:      0     633     995    1355    1849    3081 
   Size:        633     362     360     494    1232

  Fragments arranged by size: 

                1232     633     494     362     360
 
BmgBI CAC'GTC

Cuts at:      0     675    2541    3081 
   Size:        675    1866     540

  Fragments arranged by size: 

                1866     675     540
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     269     432    2326    2740    3044    3081 
   Size:        269     163    1894     414     304      37

  Fragments arranged by size: 

                1894     414     304     269     163      37
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      60    3081 
   Size:         60    3021
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     121     128     241     358     531    1204    1453 
   Size:         31      90       7     113     117     173     673     249

Cuts at:   1453    1505    2131    2353    2542    2635    3053    3081 
   Size:         52     626     222     189      93     418      28

  Fragments arranged by size: 

                 673     626     418     249     222     189     173     117
                 113      93      90      52      31      28       7
 
Bpu10I CC'TnA_GC

Cuts at:      0      86    1973    2066    2126    2672    2843    3081 
   Size:         86    1887      93      60     546     171     238

  Fragments arranged by size: 

                1887     546     238     171      93      86      60
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1159    3081 
   Size:       1159    1922
 
BsaHI Gr'CG_yC

Cuts at:      0     668    2257    2660    2928    3081 
   Size:        668    1589     403     268     153

  Fragments arranged by size: 

                1589     668     403     268     153
 
BsaWI w'CCGG_w

Cuts at:      0     503     602     929    2408    2609    3081 
   Size:        503      99     327    1479     201     472

  Fragments arranged by size: 

                1479     503     472     327     201      99
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      94     124    1198    1228    2712    2742    3081 
   Size:         94      30    1074      30    1484      30     339

  Fragments arranged by size: 

                1484    1074     339      94      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     315     854    1072    1210    1219    1399    1552    1990 
   Size:        315     539     218     138       9     180     153     438

Cuts at:   1990    2128    2689    2830    3081 
   Size:        138     561     141     251

  Fragments arranged by size: 

                 561     539     438     315     251     218     180     153
                 141     138     138       9
 
BseYI C'CCAG_C

Cuts at:      0     541    1047    1614    2171    2550    2879    3081 
   Size:        541     506     567     557     379     329     202

  Fragments arranged by size: 

                 567     557     541     506     379     329     202
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     883    1126    1622    2473    2604    3026    3081 
   Size:        328     555     243     496     851     131     422      55

  Fragments arranged by size: 

                 851     555     496     422     328     243     131      55
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     130     602     995    1355    3081 
   Size:        130     472     393     360    1726

  Fragments arranged by size: 

                1726     472     393     360     130
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      92     130     602     633     995    1355    1849 
   Size:         41      51      38     472      31     362     360     494

Cuts at:   1849    3069    3081 
   Size:       1220      12

  Fragments arranged by size: 

                1220     494     472     362     360      51      41      38
                  31      12
 
BspEI T'CCGG_A

Cuts at:      0     929    2609    3081 
   Size:        929    1680     472

  Fragments arranged by size: 

                1680     929     472
 
BspHI T'CATG_A

Cuts at:      0     701    3081 
   Size:        701    2380
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     146     506     539    1034    1092    2225    3081 
   Size:        146     360      33     495      58    1133     856

  Fragments arranged by size: 

                1133     856     495     360     146      58      33
 
BsrBI CCG'CTC

Cuts at:      0    1967    2094    2100    3081 
   Size:       1967     127       6     981

  Fragments arranged by size: 

                1967     981     127       6
 
BsrDI GCAATG_nn'

Cuts at:      0    2249    3081 
   Size:       2249     832
 
BsrFI r'CCGG_y

Cuts at:      0    2945    3081 
   Size:       2945     136
 
BssHII G'CGCG_C

Cuts at:      0     586    3081 
   Size:        586    2495
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1359    2487    3081 
   Size:       1359    1128     594

  Fragments arranged by size: 

                1359    1128     594
 
BstBI TT'CG_AA

Cuts at:      0    1677    3081 
   Size:       1677    1404
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1753    1938    2859    3081 
   Size:       1753     185     921     222

  Fragments arranged by size: 

                1753     921     222     185
 
BstYI r'GATC_y

Cuts at:      0     192     341    1787    1803    1979    2379    3081 
   Size:        192     149    1446      16     176     400     702

  Fragments arranged by size: 

                1446     702     400     192     176     149      16
 
Bsu36I CC'TnA_GG

Cuts at:      0    1535    3081 
   Size:       1535    1546
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2418    3081 
   Size:       2418     663
 
BtsI GCAGTG_nn'

Cuts at:      0    2419    2519    3000    3081 
   Size:       2419     100     481      81

  Fragments arranged by size: 

                2419     481     100      81
 
DraIII CAC_nnn'GTG

Cuts at:      0     999    1350    3081 
   Size:        999     351    1731

  Fragments arranged by size: 

                1731     999     351
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     673    2602    3081 
   Size:        673    1929     479

  Fragments arranged by size: 

                1929     673     479
 
EaeI y'GGCC_r

Cuts at:      0    1457    1940    1962    3081 
   Size:       1457     483      22    1119

  Fragments arranged by size: 

                1457    1119     483      22
 
EarI CTCTTCn'nnn_

Cuts at:      0     353    1556    2152    3081 
   Size:        353    1203     596     929

  Fragments arranged by size: 

                1203     929     596     353
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1559    3081 
   Size:       1559    1522
 
EcoICRI GAG'CTC

Cuts at:      0     128    3081 
   Size:        128    2953
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     121     128     241     358     531     655    1204 
   Size:         31      90       7     113     117     173     124     549

Cuts at:   1204    1453    1505    1749    2131    2133    2308    2353    2542 
   Size:        249      52     244     382       2     175      45     189

Cuts at:   2542    2635    3053    3081 
   Size:         93     418      28

  Fragments arranged by size: 

                 549     418     382     249     244     189     175     173
                 124     117     113      93      90      52      45      31
                  28       7       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     513    1186    1954    3081 
   Size:        513     673     768    1127

  Fragments arranged by size: 

                1127     768     673     513
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     528     629     630    1428    1452    1468    1542 
   Size:         27     501     101       1     798      24      16      74

Cuts at:   1542    1797    1845    1846    3073    3081 
   Size:        255      48       1    1227       8

  Fragments arranged by size: 

                1227     798     501     255     101      74      48      27
                  24      16       8       1       1
 
HaeII r_GCGC'y

Cuts at:      0      20     548    2931    3081 
   Size:         20     528    2383     150

  Fragments arranged by size: 

                2383     528     150      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     412     444    1198    1230    2364    2383    2396    2415 
   Size:        412      32     754      32    1134      19      13      19

Cuts at:   2415    2712    2744    3081 
   Size:        297      32     337

  Fragments arranged by size: 

                1134     754     412     337     297      32      32      32
                  19      19      13
 
HincII GTy'rAC

Cuts at:      0     317    1655    2168    3081 
   Size:        317    1338     513     913

  Fragments arranged by size: 

                1338     913     513     317
 
HindIII A'AGCT_T

Cuts at:      0    1231    3081 
   Size:       1231    1850
 
KasI G'GCGC_C

Cuts at:      0    2927    3081 
   Size:       2927     154
 
KpnI G_GTAC'C

Cuts at:      0    2975    3081 
   Size:       2975     106
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1133    1181    1526    1895    2279    3081 
   Size:       1133      48     345     369     384     802

  Fragments arranged by size: 

                1133     802     384     369     345      48
 
MscI TGG'CCA

Cuts at:      0    1459    1942    3081 
   Size:       1459     483    1139

  Fragments arranged by size: 

                1459    1139     483
 
MslI CAynn'nnrTG

Cuts at:      0     743     988    3081 
   Size:        743     245    2093

  Fragments arranged by size: 

                2093     743     245
 
MspA1I CmG'CkG

Cuts at:      0     823    1287    1296    2125    2655    2838    2883    3081 
   Size:        823     464       9     829     530     183      45     198

  Fragments arranged by size: 

                 829     823     530     464     198     183      45       9
            
 
NarI GG'CG_CC

Cuts at:      0    2928    3081 
   Size:       2928     153
 
NdeI CA'TA_TG

Cuts at:      0    1329    3081 
   Size:       1329    1752
 
NspI r_CATG'y

Cuts at:      0     972    1213    1494    3081 
   Size:        972     241     281    1587

  Fragments arranged by size: 

                1587     972     281     241
 
PasI CC'CwG_GG

Cuts at:      0     619    3081 
   Size:        619    2462
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2178    2376    3012    3081 
   Size:       2178     198     636      69

  Fragments arranged by size: 

                2178     636     198      69
 
PfoI T'CCnGG_A

Cuts at:      0     508    2207    3081 
   Size:        508    1699     874

  Fragments arranged by size: 

                1699     874     508
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      94     126    3081 
   Size:         94      32    2955

  Fragments arranged by size: 

                2955      94      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      27     528    1542    1797    3081 
   Size:         27     501    1014     255    1284

  Fragments arranged by size: 

                1284    1014     501     255      27
 
PshAI GACnn'nnGTC

Cuts at:      0    1607    3081 
   Size:       1607    1474
 
PspOMI G'GGCC_C

Cuts at:      0     629    1845    3081 
   Size:        629    1216    1236

  Fragments arranged by size: 

                1236    1216     629
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     697     729    3081 
   Size:        697      32    2352

  Fragments arranged by size: 

                2352     697      32
 
PstI C_TGCA'G

Cuts at:      0     536    1031    1070    1103    1476    1987    2222    2511 
   Size:        536     495      39      33     373     511     235     289

Cuts at:   2511    3081 
   Size:        570

  Fragments arranged by size: 

                 570     536     511     495     373     289     235      39
                  33
 
PvuII CAG'CTG

Cuts at:      0     823    1287    2125    2838    3081 
   Size:        823     464     838     713     243

  Fragments arranged by size: 

                 838     823     713     464     243
 
SacI G_AGCT'C

Cuts at:      0     130    3081 
   Size:        130    2951
 
SanDI GG'GwC_CC

Cuts at:      0      27    3081 
   Size:         27    3054
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2152    3081 
   Size:       2152     929
 
ScaI AGT'ACT

Cuts at:      0     326    3081 
   Size:        326    2755
 
SexAI A'CCwGG_T

Cuts at:      0     725    1346    3081 
   Size:        725     621    1735

  Fragments arranged by size: 

                1735     725     621
 
SfcI C'TryA_G

Cuts at:      0     532    1027    1066    1099    1291    1472    1983    2218 
   Size:        532     495      39      33     192     181     511     235

Cuts at:   2218    2507    3081 
   Size:        289     574

  Fragments arranged by size: 

                 574     532     511     495     289     235     192     181
                  39      33
 
SfoI GGC'GCC

Cuts at:      0    2929    3081 
   Size:       2929     152
 
SmaI CCC'GGG

Cuts at:      0      32    3081 
   Size:         32    3049
 
SphI G_CATG'C

Cuts at:      0     972    1494    3081 
   Size:        972     522    1587

  Fragments arranged by size: 

                1587     972     522
 
SspI AAT'ATT

Cuts at:      0     948    3081 
   Size:        948    2133
 
StuI AGG'CCT

Cuts at:      0     404    1390    2680    2996    3081 
   Size:        404     986    1290     316      85

  Fragments arranged by size: 

                1290     986     404     316      85
 
StyI C'CwwG_G

Cuts at:      0     787    2936    3081 
   Size:        787    2149     145

  Fragments arranged by size: 

                2149     787     145
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     356     360     555    3081 
   Size:        356       4     195    2526

  Fragments arranged by size: 

                2526     356     195       4
 
TatI w'GTAC_w

Cuts at:      0     324     411    3081 
   Size:        324      87    2670

  Fragments arranged by size: 

                2670     324      87
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2179    3081 
   Size:       2179     902
 
XmaI C'CCGG_G

Cuts at:      0      30    3081 
   Size:         30    3051
 
XmnI GAAnn'nnTTC

Cuts at:      0     292    3081 
   Size:        292    2789
 
ZraI GAC'GTC

Cuts at:      0     669    3081 
   Size:        669    2412
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI    BstXI
    BstYI   Bsu36I    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI
     NdeI     NspI     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI
   PspOMI     PsrI     PstI    PvuII     SacI    SanDI     SapI     ScaI
    SexAI     SfcI     SfoI     SmaI     SphI     SspI     StuI     StyI
    TaqII     TatI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AscI
     AseI    AsiSI    AvrII    BamHI     BclI    BfrBI     BmtI    BpuEI
    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BsmBI    BsrGI    BssSI
   BstEII  BstZ17I     BtgI     ClaI    CspCI     DraI     EagI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI     HpaI     MfeI     MluI
     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PsiI    PspXI     PvuI    RsrII    SacII
     SalI     SbfI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SwaI     TstI  Tth111I     XbaI     XhoI
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