Restriction map for
pF1KSDA2013
(Linear) (Six-base) MAPSORT of: KIAA2013.seq Check: 918 from: 1 to: 2001
KIAA2013.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 152 1697 2001
Size: 152 1545 304
Fragments arranged by size:
1545 304 152
AccI GT'mk_AC
Cuts at: 0 602 1174 2001
Size: 602 572 827
Fragments arranged by size:
827 602 572
Acc65I G'GTAC_C
Cuts at: 0 246 2001
Size: 246 1755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 1008 1185 1860 2001
Size: 425 583 177 675 141
Fragments arranged by size:
675 583 425 177 141
AflIII A'CryG_T
Cuts at: 0 1521 1523 2001
Size: 1521 2 478
Fragments arranged by size:
1521 478 2
AhdI GACnn_n'nnGTC
Cuts at: 0 598 2001
Size: 598 1403
AlwNI CAG_nnn'CTG
Cuts at: 0 484 1269 1698 2001
Size: 484 785 429 303
Fragments arranged by size:
785 484 429 303
ApaI G_GGCC'C
Cuts at: 0 57 512 668 2001
Size: 57 455 156 1333
Fragments arranged by size:
1333 455 156 57
ApaLI G'TGCA_C
Cuts at: 0 775 865 2001
Size: 775 90 1136
Fragments arranged by size:
1136 775 90
AvaI C'yCGr_G
Cuts at: 0 331 505 1762 1913 2001
Size: 331 174 1257 151 88
Fragments arranged by size:
1257 331 174 151 88
BanI G'GyrC_C
Cuts at: 0 172 246 354 1336 1608 2001
Size: 172 74 108 982 272 393
Fragments arranged by size:
982 393 272 172 108 74
BanII G_rGCy'C
Cuts at: 0 57 347 512 668 923 965 1017 2001
Size: 57 290 165 156 255 42 52 984
Fragments arranged by size:
984 290 255 165 156 57 52 42
BbeI G_GCGC'C
Cuts at: 0 176 358 1612 2001
Size: 176 182 1254 389
Fragments arranged by size:
1254 389 182 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 203 2001
Size: 169 34 1798
Fragments arranged by size:
1798 169 34
BfrBI ATG'CAT
Cuts at: 0 1444 2001
Size: 1444 557
BglI GCCn_nnn'nGGC
Cuts at: 0 77 277 688 1245 1492 1570 1729 2001
Size: 77 200 411 557 247 78 159 272
Fragments arranged by size:
557 411 272 247 200 159 78 77
BglII A'GATC_T
Cuts at: 0 1556 2001
Size: 1556 445
BlpI GC'TnA_GC
Cuts at: 0 391 1016 1132 1366 2001
Size: 391 625 116 234 635
Fragments arranged by size:
635 625 391 234 116
Bme1580I G_kGCm'C
Cuts at: 0 57 371 512 668 779 869 1211 1341
Size: 57 314 141 156 111 90 342 130
Cuts at: 1341 2001
Size: 660
Fragments arranged by size:
660 342 314 156 141 130 111 90
57
BmgBI CAC'GTC
Cuts at: 0 303 600 870 1425 2001
Size: 303 297 270 555 576
Fragments arranged by size:
576 555 303 297 270
BmrI ACTGGGnnnn_n'
Cuts at: 0 776 2001
Size: 776 1225
BmtI G_CTAG'C
Cuts at: 0 1960 2001
Size: 1960 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1620 2001
Size: 1620 381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 281 431 727 979 1285 1387 1880
Size: 217 64 150 296 252 306 102 493
Cuts at: 1880 2001
Size: 121
Fragments arranged by size:
493 306 296 252 217 150 121 102
64
Bpu10I CC'TnA_GC
Cuts at: 0 1144 2001
Size: 1144 857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 907 1702 2001
Size: 4 903 795 299
Fragments arranged by size:
903 795 299 4
BsaI GGTCTCn'nnnn_
Cuts at: 0 713 1154 2001
Size: 713 441 847
Fragments arranged by size:
847 713 441
BsaAI yAC'GTr
Cuts at: 0 1524 2001
Size: 1524 477
BsaHI Gr'CG_yC
Cuts at: 0 173 355 1609 2001
Size: 173 182 1254 392
Fragments arranged by size:
1254 392 182 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 404 434 1093 1123 2001
Size: 404 30 659 30 878
Fragments arranged by size:
878 659 404 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 724 997 2001
Size: 55 669 273 1004
Fragments arranged by size:
1004 669 273 55
BseYI C'CCAG_C
Cuts at: 0 50 252 468 678 1751 2001
Size: 50 202 216 210 1073 250
Fragments arranged by size:
1073 250 216 210 202 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 397 1414 1696 2001
Size: 124 273 1017 282 305
Fragments arranged by size:
1017 305 282 273 124
BsiEI CG_ry'CG
Cuts at: 0 546 2001
Size: 546 1455
BsiHKAI G_wGCw'C
Cuts at: 0 779 869 899 923 965 1367 1745 2001
Size: 779 90 30 24 42 402 378 256
Fragments arranged by size:
779 402 378 256 90 42 30 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 525 841 876 2001
Size: 525 316 35 1125
Fragments arranged by size:
1125 525 316 35
Bsp1286I G_dGCh'C
Cuts at: 0 57 347 371 512 668 779 869 899
Size: 57 290 24 141 156 111 90 30
Cuts at: 899 923 965 1017 1211 1341 1367 1745 2001
Size: 24 42 52 194 130 26 378 256
Fragments arranged by size:
378 290 256 194 156 141 130 111
90 57 52 42 30 26 24 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 614 1697 1706 2001
Size: 152 462 1083 9 295
Fragments arranged by size:
1083 462 295 152 9
BsrBI CCG'CTC
Cuts at: 0 339 2001
Size: 339 1662
BsrDI GCAATG_nn'
Cuts at: 0 1444 2001
Size: 1444 557
BsrFI r'CCGG_y
Cuts at: 0 267 499 673 746 2001
Size: 267 232 174 73 1255
Fragments arranged by size:
1255 267 232 174 73
BssHII G'CGCG_C
Cuts at: 0 116 395 2001
Size: 116 279 1606
Fragments arranged by size:
1606 279 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 147 1269 1698 1710 2001
Size: 147 1122 429 12 291
Fragments arranged by size:
1122 429 291 147 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 771 804 1309 2001
Size: 705 66 33 505 692
Fragments arranged by size:
705 692 505 66 33
BstYI r'GATC_y
Cuts at: 0 1265 1556 2001
Size: 1265 291 445
Fragments arranged by size:
1265 445 291
BtgI C'CryG_G
Cuts at: 0 159 350 491 533 632 1529 1539 1613
Size: 159 191 141 42 99 897 10 74
Cuts at: 1613 2001
Size: 388
Fragments arranged by size:
897 388 191 159 141 99 74 42
10
BtsI GCAGTG_nn'
Cuts at: 0 668 1126 1194 1657 2001
Size: 668 458 68 463 344
Fragments arranged by size:
668 463 458 344 68
DraIII CAC_nnn'GTG
Cuts at: 0 783 2001
Size: 783 1218
EaeI y'GGCC_r
Cuts at: 0 203 278 494 543 644 791 1237 1484
Size: 203 75 216 49 101 147 446 247
Cuts at: 1484 1542 2001
Size: 58 459
Fragments arranged by size:
459 446 247 216 203 147 101 75
58 49
EagI C'GGCC_G
Cuts at: 0 543 2001
Size: 543 1458
EarI CTCTTCn'nnn_
Cuts at: 0 1027 1783 1816 1825 1831 1879 2001
Size: 1027 756 33 9 6 48 122
Fragments arranged by size:
1027 756 122 48 33 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 997 1510 2001
Size: 997 513 491
Fragments arranged by size:
997 513 491
EcoICRI GAG'CTC
Cuts at: 0 921 963 2001
Size: 921 42 1038
Fragments arranged by size:
1038 921 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 281 425 431 727 979 1008 1185
Size: 217 64 144 6 296 252 29 177
Cuts at: 1185 1285 1387 1860 1880 2001
Size: 100 102 473 20 121
Fragments arranged by size:
473 296 252 217 177 144 121 102
100 64 29 20 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 709 2001
Size: 709 1292
EcoO109I rG'GnC_Cy
Cuts at: 0 256 473 481 664 1695 1758 2001
Size: 256 217 8 183 1031 63 243
Fragments arranged by size:
1031 256 243 217 183 63 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1890 2001
Size: 1890 111
HaeII r_GCGC'y
Cuts at: 0 176 358 931 1612 2001
Size: 176 182 573 681 389
Fragments arranged by size:
681 573 389 182 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 584 616 2001
Size: 584 32 1385
Fragments arranged by size:
1385 584 32
HincII GTy'rAC
Cuts at: 0 1175 2001
Size: 1175 826
KasI G'GCGC_C
Cuts at: 0 172 354 1608 2001
Size: 172 182 1254 393
Fragments arranged by size:
1254 393 182 172
KpnI G_GTAC'C
Cuts at: 0 250 2001
Size: 250 1751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 2001
Size: 696 1305
MscI TGG'CCA
Cuts at: 0 280 1544 2001
Size: 280 1264 457
Fragments arranged by size:
1264 457 280
MspA1I CmG'CkG
Cuts at: 0 15 50 161 252 381 390 468 535
Size: 15 35 111 91 129 9 78 67
Cuts at: 535 840 979 1205 2001
Size: 305 139 226 796
Fragments arranged by size:
796 305 226 139 129 111 91 78
67 35 15 9
NaeI GCC'GGC
Cuts at: 0 269 501 675 2001
Size: 269 232 174 1326
Fragments arranged by size:
1326 269 232 174
NarI GG'CG_CC
Cuts at: 0 173 355 1609 2001
Size: 173 182 1254 392
Fragments arranged by size:
1254 392 182 173
NgoMIV G'CCGG_C
Cuts at: 0 267 499 673 2001
Size: 267 232 174 1328
Fragments arranged by size:
1328 267 232 174
NheI G'CTAG_C
Cuts at: 0 1956 2001
Size: 1956 45
NsiI A_TGCA'T
Cuts at: 0 1446 2001
Size: 1446 555
NspI r_CATG'y
Cuts at: 0 975 1107 2001
Size: 975 132 894
Fragments arranged by size:
975 894 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 484 2001
Size: 484 1517
PfoI T'CCnGG_A
Cuts at: 0 258 2001
Size: 258 1743
PmlI CAC'GTG
Cuts at: 0 1524 2001
Size: 1524 477
PpuMI rG'GwC_Cy
Cuts at: 0 256 473 481 1695 1758 2001
Size: 256 217 8 1214 63 243
Fragments arranged by size:
1214 256 243 217 63 8
PshAI GACnn'nnGTC
Cuts at: 0 39 2001
Size: 39 1962
PspOMI G'GGCC_C
Cuts at: 0 53 508 664 2001
Size: 53 455 156 1337
Fragments arranged by size:
1337 455 156 53
PstI C_TGCA'G
Cuts at: 0 11 467 611 656 935 1274 1355 1385
Size: 11 456 144 45 279 339 81 30
Cuts at: 1385 1694 2001
Size: 309 307
Fragments arranged by size:
456 339 309 307 279 144 81 45
30 11
PvuII CAG'CTG
Cuts at: 0 50 381 468 840 2001
Size: 50 331 87 372 1161
Fragments arranged by size:
1161 372 331 87 50
SacI G_AGCT'C
Cuts at: 0 923 965 2001
Size: 923 42 1036
Fragments arranged by size:
1036 923 42
SacII CC_GC'GG
Cuts at: 0 162 536 2001
Size: 162 374 1465
Fragments arranged by size:
1465 374 162
SalI G'TCGA_C
Cuts at: 0 1173 2001
Size: 1173 828
SanDI GG'GwC_CC
Cuts at: 0 473 2001
Size: 473 1528
SapI GCTCTTCn'nnn_
Cuts at: 0 1027 2001
Size: 1027 974
SbfI CC_TGCA'GG
Cuts at: 0 1355 1694 2001
Size: 1355 339 307
Fragments arranged by size:
1355 339 307
ScaI AGT'ACT
Cuts at: 0 1849 2001
Size: 1849 152
SexAI A'CCwGG_T
Cuts at: 0 779 2001
Size: 779 1222
SfcI C'TryA_G
Cuts at: 0 7 463 607 652 931 1270 1351 1381
Size: 7 456 144 45 279 339 81 30
Cuts at: 1381 1690 2001
Size: 309 311
Fragments arranged by size:
456 339 311 309 279 144 81 45
30 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 277 1729 2001
Size: 277 1452 272
Fragments arranged by size:
1452 277 272
SfoI GGC'GCC
Cuts at: 0 174 356 1610 2001
Size: 174 182 1254 391
Fragments arranged by size:
1254 391 182 174
SmaI CCC'GGG
Cuts at: 0 333 507 1764 2001
Size: 333 174 1257 237
Fragments arranged by size:
1257 333 237 174
SmlI C'TyrA_G
Cuts at: 0 19 922 1717 2001
Size: 19 903 795 284
Fragments arranged by size:
903 795 284 19
StuI AGG'CCT
Cuts at: 0 691 1919 1980 2001
Size: 691 1228 61 21
Fragments arranged by size:
1228 691 61 21
StyI C'CwwG_G
Cuts at: 0 686 914 1920 2001
Size: 686 228 1006 81
Fragments arranged by size:
1006 686 228 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 849 1075 1324 1620 1983 2001
Size: 312 537 226 249 296 363 18
Fragments arranged by size:
537 363 312 296 249 226 18
TatI w'GTAC_w
Cuts at: 0 1847 2001
Size: 1847 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 404 436 2001
Size: 404 32 1565
Fragments arranged by size:
1565 404 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 639 837 1069 2001
Size: 288 351 198 232 932
Fragments arranged by size:
932 351 288 232 198
XmaI C'CCGG_G
Cuts at: 0 331 505 1762 2001
Size: 331 174 1257 239
Fragments arranged by size:
1257 331 239 174
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MspA1I NaeI NarI NgoMIV NheI NsiI NspI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BciVI BclI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI MslI NcoI NdeI
NotI NruI PacI PasI PciI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI
ZraI