Restriction map for
pF1KA2001
(Linear) (Six-base) MAPSORT of: KIAA2001.seq Check: 812 from: 1 to: 1707
KIAA2001.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AatII G_ACGT'C
Cuts at: 0 1638 1707
Size: 1638 69
AccI GT'mk_AC
Cuts at: 0 482 1707
Size: 482 1225
AflIII A'CryG_T
Cuts at: 0 702 1427 1707
Size: 702 725 280
Fragments arranged by size:
725 702 280
AlwNI CAG_nnn'CTG
Cuts at: 0 780 1330 1707
Size: 780 550 377
Fragments arranged by size:
780 550 377
ApaI G_GGCC'C
Cuts at: 0 350 1707
Size: 350 1357
ApoI r'AATT_y
Cuts at: 0 493 679 844 1697 1707
Size: 493 186 165 853 10
Fragments arranged by size:
853 493 186 165 10
AvaI C'yCGr_G
Cuts at: 0 106 238 544 1707
Size: 106 132 306 1163
Fragments arranged by size:
1163 306 132 106
BamHI G'GATC_C
Cuts at: 0 1320 1707
Size: 1320 387
BanI G'GyrC_C
Cuts at: 0 435 550 1558 1707
Size: 435 115 1008 149
Fragments arranged by size:
1008 435 149 115
BanII G_rGCy'C
Cuts at: 0 117 287 350 464 863 1707
Size: 117 170 63 114 399 844
Fragments arranged by size:
844 399 170 117 114 63
BbeI G_GCGC'C
Cuts at: 0 554 1707
Size: 554 1153
BbsI GAAGACnn'nnnn_
Cuts at: 0 1049 1638 1707
Size: 1049 589 69
Fragments arranged by size:
1049 589 69
BglI GCCn_nnn'nGGC
Cuts at: 0 1556 1707
Size: 1556 151
Bme1580I G_kGCm'C
Cuts at: 0 185 350 1707
Size: 185 165 1357
Fragments arranged by size:
1357 185 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 472 612 1707
Size: 472 140 1095
Fragments arranged by size:
1095 472 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 135 463 980 1707
Size: 85 50 328 517 727
Fragments arranged by size:
727 517 328 85 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1707
Size: 715 992
BsaI GGTCTCn'nnnn_
Cuts at: 0 467 505 1330 1707
Size: 467 38 825 377
Fragments arranged by size:
825 467 377 38
BsaAI yAC'GTr
Cuts at: 0 703 1707
Size: 703 1004
BsaHI Gr'CG_yC
Cuts at: 0 10 551 1635 1707
Size: 10 541 1084 72
Fragments arranged by size:
1084 541 72 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 464 494 1707
Size: 464 30 1213
Fragments arranged by size:
1213 464 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 1144 1147 1150 1195 1198 1201 1228
Size: 211 933 3 3 45 3 3 27
Cuts at: 1228 1231 1234 1237 1258 1348 1369 1372 1707
Size: 3 3 3 21 90 21 3 335
Fragments arranged by size:
933 335 211 90 45 27 21 21
3 3 3 3 3 3 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 863 1707
Size: 863 844
BsmI GAATG_Cn'
Cuts at: 0 90 1707
Size: 90 1617
Bsp1286I G_dGCh'C
Cuts at: 0 117 185 287 350 464 863 1707
Size: 117 68 102 63 114 399 844
Fragments arranged by size:
844 399 117 114 102 68 63
BsrBI CCG'CTC
Cuts at: 0 447 558 954 1707
Size: 447 111 396 753
Fragments arranged by size:
753 447 396 111
BsrDI GCAATG_nn'
Cuts at: 0 150 1707
Size: 150 1557
BsrFI r'CCGG_y
Cuts at: 0 263 384 1707
Size: 263 121 1323
Fragments arranged by size:
1323 263 121
BssSI C'ACGA_G
Cuts at: 0 1688 1707
Size: 1688 19
BstBI TT'CG_AA
Cuts at: 0 966 1629 1707
Size: 966 663 78
Fragments arranged by size:
966 663 78
BstEII G'GTnAC_C
Cuts at: 0 615 1459 1707
Size: 615 844 248
Fragments arranged by size:
844 615 248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1444 1707
Size: 1444 263
BstYI r'GATC_y
Cuts at: 0 1320 1707
Size: 1320 387
EaeI y'GGCC_r
Cuts at: 0 119 166 881 1692 1707
Size: 119 47 715 811 15
Fragments arranged by size:
811 715 119 47 15
EarI CTCTTCn'nnn_
Cuts at: 0 1016 1166 1187 1707
Size: 1016 150 21 520
Fragments arranged by size:
1016 520 150 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 386 1642 1707
Size: 386 1256 65
Fragments arranged by size:
1256 386 65
EcoICRI GAG'CTC
Cuts at: 0 861 1707
Size: 861 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 135 463 980 1707
Size: 85 50 328 517 727
Fragments arranged by size:
727 517 328 85 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 193 1707
Size: 121 72 1514
Fragments arranged by size:
1514 121 72
EcoRI G'AATT_C
Cuts at: 0 493 679 844 1697 1707
Size: 493 186 165 853 10
Fragments arranged by size:
853 493 186 165 10
HaeII r_GCGC'y
Cuts at: 0 391 554 1707
Size: 391 163 1153
Fragments arranged by size:
1153 391 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 344 464 496 1707
Size: 280 32 32 120 32 1211
Fragments arranged by size:
1211 280 120 32 32 32
HincII GTy'rAC
Cuts at: 0 872 1707
Size: 872 835
KasI G'GCGC_C
Cuts at: 0 550 1707
Size: 550 1157
MluI A'CGCG_T
Cuts at: 0 1427 1707
Size: 1427 280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1707
Size: 821 886
MscI TGG'CCA
Cuts at: 0 168 1707
Size: 168 1539
MspA1I CmG'CkG
Cuts at: 0 164 352 441 1707
Size: 164 188 89 1266
Fragments arranged by size:
1266 188 164 89
NaeI GCC'GGC
Cuts at: 0 265 386 1707
Size: 265 121 1321
Fragments arranged by size:
1321 265 121
NarI GG'CG_CC
Cuts at: 0 551 1707
Size: 551 1156
NgoMIV G'CCGG_C
Cuts at: 0 263 384 1707
Size: 263 121 1323
Fragments arranged by size:
1323 263 121
NspI r_CATG'y
Cuts at: 0 643 1707
Size: 643 1064
PflMI CCAn_nnn'nTGG
Cuts at: 0 602 1707
Size: 602 1105
PfoI T'CCnGG_A
Cuts at: 0 323 659 1707
Size: 323 336 1048
Fragments arranged by size:
1048 336 323
PmlI CAC'GTG
Cuts at: 0 703 1707
Size: 703 1004
PspOMI G'GGCC_C
Cuts at: 0 346 1707
Size: 346 1361
SacI G_AGCT'C
Cuts at: 0 863 1707
Size: 863 844
ScaI AGT'ACT
Cuts at: 0 1027 1707
Size: 1027 680
SfcI C'TryA_G
Cuts at: 0 1029 1575 1707
Size: 1029 546 132
Fragments arranged by size:
1029 546 132
SfoI GGC'GCC
Cuts at: 0 552 1707
Size: 552 1155
SgrAI Cr'CCGG_yG
Cuts at: 0 384 1707
Size: 384 1323
SmaI CCC'GGG
Cuts at: 0 240 546 1707
Size: 240 306 1161
Fragments arranged by size:
1161 306 240
SmlI C'TyrA_G
Cuts at: 0 730 1707
Size: 730 977
SphI G_CATG'C
Cuts at: 0 643 1707
Size: 643 1064
StuI AGG'CCT
Cuts at: 0 836 1550 1707
Size: 836 714 157
Fragments arranged by size:
836 714 157
StyI C'CwwG_G
Cuts at: 0 596 1076 1707
Size: 596 480 631
Fragments arranged by size:
631 596 480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 603 634 1471 1707
Size: 603 31 837 236
Fragments arranged by size:
837 603 236 31
TatI w'GTAC_w
Cuts at: 0 1025 1707
Size: 1025 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1417 1449 1707
Size: 1417 32 258
Fragments arranged by size:
1417 258 32
Tth111I GACn'n_nGTC
Cuts at: 0 478 1707
Size: 478 1229
XmaI C'CCGG_G
Cuts at: 0 238 544 1707
Size: 238 306 1163
Fragments arranged by size:
1163 306 238
XmnI GAAnn'nnTTC
Cuts at: 0 233 1550 1707
Size: 233 1317 157
Fragments arranged by size:
1317 233 157
ZraI GAC'GTC
Cuts at: 0 1636 1707
Size: 1636 71
Enzymes that do cut:
AatII AccI AflIII AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsiHKAI BsmI
Bsp1286I BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI
BstYI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
HaeII Hin4I HincII KasI MluI MmeI MscI MspA1I
NaeI NarI NgoMIV NspI PflMI PfoI PmlI PspOMI
SacI ScaI SfcI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XmaI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaBI BsaWI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII
BstAPI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoO109I EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SnaBI SpeI SrfI SspI SwaI
XbaI XcmI XhoI