Restriction map for
pF1KA1998
(Linear) (Six-base) MAPSORT of: KIAA1998.seq Check: 6467 from: 1 to: 5115
KIAA1998.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4358 5115
Size: 4358 757
AatII G_ACGT'C
Cuts at: 0 653 3755 5115
Size: 653 3102 1360
Fragments arranged by size:
3102 1360 653
AccI GT'mk_AC
Cuts at: 0 1118 4829 5115
Size: 1118 3711 286
Fragments arranged by size:
3711 1118 286
Acc65I G'GTAC_C
Cuts at: 0 2281 5115
Size: 2281 2834
AclI AA'CG_TT
Cuts at: 0 158 5115
Size: 158 4957
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 175 923 1337 2283 2607 3593 3644
Size: 101 74 748 414 946 324 986 51
Cuts at: 3644 4141 4312 5115
Size: 497 171 803
Fragments arranged by size:
986 946 803 748 497 414 324 171
101 74 51
AfeI AGC'GCT
Cuts at: 0 3089 5115
Size: 3089 2026
AflIII A'CryG_T
Cuts at: 0 126 3216 5115
Size: 126 3090 1899
Fragments arranged by size:
3090 1899 126
AhdI GACnn_n'nnGTC
Cuts at: 0 2554 4275 5115
Size: 2554 1721 840
Fragments arranged by size:
2554 1721 840
AleI CACnn'nnGTG
Cuts at: 0 2037 5115
Size: 2037 3078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3734 5115
Size: 3734 1381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1515 1547 5115
Size: 1515 32 3568
Fragments arranged by size:
3568 1515 32
AlwNI CAG_nnn'CTG
Cuts at: 0 351 901 1840 2601 3237 4536 4578 4888
Size: 351 550 939 761 636 1299 42 310
Cuts at: 4888 5115
Size: 227
Fragments arranged by size:
1299 939 761 636 550 351 310 227
42
ApaI G_GGCC'C
Cuts at: 0 4569 5115
Size: 4569 546
ApaLI G'TGCA_C
Cuts at: 0 2062 4318 5115
Size: 2062 2256 797
Fragments arranged by size:
2256 2062 797
ApoI r'AATT_y
Cuts at: 0 657 918 1688 1790 2104 2151 2675 2772
Size: 657 261 770 102 314 47 524 97
Cuts at: 2772 2857 2925 2972 3500 3641 3683 4086 4131
Size: 85 68 47 528 141 42 403 45
Cuts at: 4131 4992 5115
Size: 861 123
Fragments arranged by size:
861 770 657 528 524 403 314 261
141 123 102 97 85 68 47 47
45 42
AseI AT'TA_AT
Cuts at: 0 1172 5115
Size: 1172 3943
AvaI C'yCGr_G
Cuts at: 0 555 689 4021 4642 5115
Size: 555 134 3332 621 473
Fragments arranged by size:
3332 621 555 473 134
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2797 2830 5115
Size: 2797 33 2285
Fragments arranged by size:
2797 2285 33
BamHI G'GATC_C
Cuts at: 0 2505 3545 4017 4025 5115
Size: 2505 1040 472 8 1090
Fragments arranged by size:
2505 1090 1040 472 8
BanI G'GyrC_C
Cuts at: 0 595 2281 3880 5115
Size: 595 1686 1599 1235
Fragments arranged by size:
1686 1599 1235 595
BanII G_rGCy'C
Cuts at: 0 256 407 1516 2392 3767 4569 5115
Size: 256 151 1109 876 1375 802 546
Fragments arranged by size:
1375 1109 876 802 546 256 151
BbeI G_GCGC'C
Cuts at: 0 3884 5115
Size: 3884 1231
BbsI GAAGACnn'nnnn_
Cuts at: 0 653 1007 3614 5115
Size: 653 354 2607 1501
Fragments arranged by size:
2607 1501 653 354
BbvCI CC'TCA_GC
Cuts at: 0 2455 4519 5115
Size: 2455 2064 596
Fragments arranged by size:
2455 2064 596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 304 338 577 611 5115
Size: 304 34 239 34 4504
Fragments arranged by size:
4504 304 239 34 34
BclI T'GATC_A
Cuts at: 0 1140 3387 3464 5115
Size: 1140 2247 77 1651
Fragments arranged by size:
2247 1651 1140 77
BfrBI ATG'CAT
Cuts at: 0 2586 2984 5115
Size: 2586 398 2131
Fragments arranged by size:
2586 2131 398
BglI GCCn_nnn'nGGC
Cuts at: 0 373 3634 4583 5115
Size: 373 3261 949 532
Fragments arranged by size:
3261 949 532 373
BglII A'GATC_T
Cuts at: 0 2618 5115
Size: 2618 2497
Bme1580I G_kGCm'C
Cuts at: 0 2066 4322 4569 5115
Size: 2066 2256 247 546
Fragments arranged by size:
2256 2066 546 247
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1265 1535 5115
Size: 531 734 270 3580
Fragments arranged by size:
3580 734 531 270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 715 5115
Size: 715 4400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 870 1484 1575 1670 2887 4195 4324
Size: 148 722 614 91 95 1217 1308 129
Cuts at: 4324 4468 4474 4531 5102 5115
Size: 144 6 57 571 13
Fragments arranged by size:
1308 1217 722 614 571 148 144 129
95 91 57 13 6
Bpu10I CC'TnA_GC
Cuts at: 0 2455 4519 5115
Size: 2455 2064 596
Fragments arranged by size:
2455 2064 596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1503 3184 4819 5115
Size: 1503 1681 1635 296
Fragments arranged by size:
1681 1635 1503 296
BsaI GGTCTCn'nnnn_
Cuts at: 0 1511 2188 2363 5115
Size: 1511 677 175 2752
Fragments arranged by size:
2752 1511 677 175
BsaAI yAC'GTr
Cuts at: 0 273 622 5115
Size: 273 349 4493
Fragments arranged by size:
4493 349 273
BsaBI GATnn'nnATC
Cuts at: 0 43 1022 4016 5115
Size: 43 979 2994 1099
Fragments arranged by size:
2994 1099 979 43
BsaHI Gr'CG_yC
Cuts at: 0 650 1448 2964 3752 3881 5115
Size: 650 798 1516 788 129 1234
Fragments arranged by size:
1516 1234 798 788 650 129
BsaWI w'CCGG_w
Cuts at: 0 240 803 1575 5115
Size: 240 563 772 3540
Fragments arranged by size:
3540 772 563 240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3905 3935 4213 4243 4417 4423 4447 4453
Size: 3905 30 278 30 174 6 24 6
Cuts at: 4453 5115
Size: 662
Fragments arranged by size:
3905 662 278 174 30 30 24 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 1506 2656 3460 3715 4318 4462 4623
Size: 415 1091 1150 804 255 603 144 161
Cuts at: 4623 5115
Size: 492
Fragments arranged by size:
1150 1091 804 603 492 415 255 161
144
BseYI C'CCAG_C
Cuts at: 0 1322 4568 4581 5115
Size: 1322 3246 13 534
Fragments arranged by size:
3246 1322 534 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 914 5115
Size: 715 199 4201
Fragments arranged by size:
4201 715 199
BsiEI CG_ry'CG
Cuts at: 0 2787 4602 5115
Size: 2787 1815 513
Fragments arranged by size:
2787 1815 513
BsiHKAI G_wGCw'C
Cuts at: 0 384 407 1516 2066 4322 4628 5115
Size: 384 23 1109 550 2256 306 487
Fragments arranged by size:
2256 1109 550 487 384 306 23
BsmI GAATG_Cn'
Cuts at: 0 1470 2984 2986 5115
Size: 1470 1514 2 2129
Fragments arranged by size:
2129 1514 1470 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 190 3534 4059 4147 5115
Size: 190 3344 525 88 968
Fragments arranged by size:
3344 968 525 190 88
Bsp1286I G_dGCh'C
Cuts at: 0 256 384 407 1516 2066 2392 3767 4322
Size: 256 128 23 1109 550 326 1375 555
Cuts at: 4322 4569 4628 5115
Size: 247 59 487
Fragments arranged by size:
1375 1109 555 550 487 326 256 247
128 59 23
BspEI T'CCGG_A
Cuts at: 0 803 5115
Size: 803 4312
BspHI T'CATG_A
Cuts at: 0 131 1877 3945 4680 5115
Size: 131 1746 2068 735 435
Fragments arranged by size:
2068 1746 735 435 131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1833 2345 4358 5115
Size: 1833 512 2013 757
Fragments arranged by size:
2013 1833 757 512
BsrBI CCG'CTC
Cuts at: 0 3740 4422 4428 4515 5115
Size: 3740 682 6 87 600
Fragments arranged by size:
3740 682 600 87 6
BsrDI GCAATG_nn'
Cuts at: 0 1066 3655 5115
Size: 1066 2589 1460
Fragments arranged by size:
2589 1460 1066
BsrFI r'CCGG_y
Cuts at: 0 1082 4601 5115
Size: 1082 3519 514
Fragments arranged by size:
3519 1082 514
BssSI C'ACGA_G
Cuts at: 0 406 5115
Size: 406 4709
BstAPI GCAn_nnn'nTGC
Cuts at: 0 388 4578 5115
Size: 388 4190 537
Fragments arranged by size:
4190 537 388
BstBI TT'CG_AA
Cuts at: 0 4858 5115
Size: 4858 257
BstEII G'GTnAC_C
Cuts at: 0 243 4038 5115
Size: 243 3795 1077
Fragments arranged by size:
3795 1077 243
BstXI CCAn_nnnn'nTGG
Cuts at: 0 642 2319 5115
Size: 642 1677 2796
Fragments arranged by size:
2796 1677 642
BstYI r'GATC_y
Cuts at: 0 112 2505 2618 3167 3545 4017 4025 4193
Size: 112 2393 113 549 378 472 8 168
Cuts at: 4193 5115
Size: 922
Fragments arranged by size:
2393 922 549 472 378 168 113 112
8
BtgI C'CryG_G
Cuts at: 0 183 248 2384 2666 4619 5115
Size: 183 65 2136 282 1953 496
Fragments arranged by size:
2136 1953 496 282 183 65
BtsI GCAGTG_nn'
Cuts at: 0 382 1187 1639 1999 2032 2044 2466 3866
Size: 382 805 452 360 33 12 422 1400
Cuts at: 3866 5115
Size: 1249
Fragments arranged by size:
1400 1249 805 452 422 382 360 33
12
ClaI AT'CG_AT
Cuts at: 0 4662 5115
Size: 4662 453
DraI TTT'AAA
Cuts at: 0 2895 3128 5115
Size: 2895 233 1987
Fragments arranged by size:
2895 1987 233
DraIII CAC_nnn'GTG
Cuts at: 0 1226 1288 5115
Size: 1226 62 3827
Fragments arranged by size:
3827 1226 62
DrdI GACnn_nn'nnGTC
Cuts at: 0 4051 5115
Size: 4051 1064
EaeI y'GGCC_r
Cuts at: 0 3635 4049 4236 4310 4599 4896 5115
Size: 3635 414 187 74 289 297 219
Fragments arranged by size:
3635 414 297 289 219 187 74
EagI C'GGCC_G
Cuts at: 0 4599 5115
Size: 4599 516
EarI CTCTTCn'nnn_
Cuts at: 0 500 584 805 902 1739 2397 3993 5115
Size: 500 84 221 97 837 658 1596 1122
Fragments arranged by size:
1596 1122 837 658 500 221 97 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3943 5115
Size: 3943 1172
EcoICRI GAG'CTC
Cuts at: 0 405 1514 5115
Size: 405 1109 3601
Fragments arranged by size:
3601 1109 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 148 175 870 923 1337 1484 1575
Size: 101 47 27 695 53 414 147 91
Cuts at: 1575 1670 2283 2607 2887 3593 3644 4141 4195
Size: 95 613 324 280 706 51 497 54
Cuts at: 4195 4312 4324 4468 4474 4531 5102 5115
Size: 117 12 144 6 57 571 13
Fragments arranged by size:
706 695 613 571 497 414 324 280
147 144 117 101 95 91 57 54
53 51 47 27 13 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3788 5115
Size: 3788 1327
EcoO109I rG'GnC_Cy
Cuts at: 0 519 4224 5115
Size: 519 3705 891
Fragments arranged by size:
3705 891 519
EcoRI G'AATT_C
Cuts at: 0 918 1790 2857 2972 5115
Size: 918 872 1067 115 2143
Fragments arranged by size:
2143 1067 918 872 115
EcoRV GAT'ATC
Cuts at: 0 1038 1161 3312 5115
Size: 1038 123 2151 1803
Fragments arranged by size:
2151 1803 1038 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 43 5115
Size: 43 5072
FspI TGC'GCA
Cuts at: 0 4366 5115
Size: 4366 749
HaeII r_GCGC'y
Cuts at: 0 3091 3884 4359 4536 5115
Size: 3091 793 475 177 579
Fragments arranged by size:
3091 793 579 475 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1881 1913 2540 2572 3905 3937 4213 4245
Size: 1881 32 627 32 1333 32 276 32
Cuts at: 4245 4261 4293 4423 4455 4662 4694 5115
Size: 16 32 130 32 207 32 421
Fragments arranged by size:
1881 1333 627 421 276 207 130 32
32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 0 2886 5115
Size: 2886 2229
HindIII A'AGCT_T
Cuts at: 0 681 1754 2295 3418 4706 5115
Size: 681 1073 541 1123 1288 409
Fragments arranged by size:
1288 1123 1073 681 541 409
HpaI GTT'AAC
Cuts at: 0 2886 5115
Size: 2886 2229
KasI G'GCGC_C
Cuts at: 0 3880 5115
Size: 3880 1235
KpnI G_GTAC'C
Cuts at: 0 2285 5115
Size: 2285 2830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1385 2196 5115
Size: 761 624 811 2919
Fragments arranged by size:
2919 811 761 624
MscI TGG'CCA
Cuts at: 0 3637 4312 5115
Size: 3637 675 803
Fragments arranged by size:
3637 803 675
MslI CAynn'nnrTG
Cuts at: 0 2037 2366 3716 4766 5115
Size: 2037 329 1350 1050 349
Fragments arranged by size:
2037 1350 1050 349 329
MspA1I CmG'CkG
Cuts at: 0 890 2345 2652 4353 4386 4621 4888 5115
Size: 890 1455 307 1701 33 235 267 227
Fragments arranged by size:
1701 1455 890 307 267 235 227 33
NaeI GCC'GGC
Cuts at: 0 1084 4603 5115
Size: 1084 3519 512
Fragments arranged by size:
3519 1084 512
NarI GG'CG_CC
Cuts at: 0 3881 5115
Size: 3881 1234
NcoI C'CATG_G
Cuts at: 0 183 248 2384 5115
Size: 183 65 2136 2731
Fragments arranged by size:
2731 2136 183 65
NgoMIV G'CCGG_C
Cuts at: 0 1082 4601 5115
Size: 1082 3519 514
Fragments arranged by size:
3519 1082 514
NruI TCG'CGA
Cuts at: 0 4283 5115
Size: 4283 832
NsiI A_TGCA'T
Cuts at: 0 2588 2986 5115
Size: 2588 398 2129
Fragments arranged by size:
2588 2129 398
NspI r_CATG'y
Cuts at: 0 130 1098 2096 3220 5115
Size: 130 968 998 1124 1895
Fragments arranged by size:
1895 1124 998 968 130
PacI TTA_AT'TAA
Cuts at: 0 3101 5115
Size: 3101 2014
PciI A'CATG_T
Cuts at: 0 126 3216 5115
Size: 126 3090 1899
Fragments arranged by size:
3090 1899 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 184 573 2179 4035 4203 4536 5115
Size: 184 389 1606 1856 168 333 579
Fragments arranged by size:
1856 1606 579 389 333 184 168
PfoI T'CCnGG_A
Cuts at: 0 4490 5115
Size: 4490 625
PsiI TTA'TAA
Cuts at: 0 5023 5115
Size: 5023 92
PspOMI G'GGCC_C
Cuts at: 0 4565 5115
Size: 4565 550
PstI C_TGCA'G
Cuts at: 0 16 340 1080 1994 2039 2350 2651 4418
Size: 16 324 740 914 45 311 301 1767
Cuts at: 4418 5015 5115
Size: 597 100
Fragments arranged by size:
1767 914 740 597 324 311 301 100
45 16
PvuII CAG'CTG
Cuts at: 0 2345 2652 5115
Size: 2345 307 2463
Fragments arranged by size:
2463 2345 307
SacI G_AGCT'C
Cuts at: 0 407 1516 5115
Size: 407 1109 3599
Fragments arranged by size:
3599 1109 407
SacII CC_GC'GG
Cuts at: 0 4622 5115
Size: 4622 493
SapI GCTCTTCn'nnn_
Cuts at: 0 1739 2397 5115
Size: 1739 658 2718
Fragments arranged by size:
2718 1739 658
SfcI C'TryA_G
Cuts at: 0 12 336 1076 1256 1624 1990 2035 2346
Size: 12 324 740 180 368 366 45 311
Cuts at: 2346 2377 2402 2647 2724 3293 3557 4414 5011
Size: 31 25 245 77 569 264 857 597
Cuts at: 5011 5115
Size: 104
Fragments arranged by size:
857 740 597 569 368 366 324 311
264 245 180 104 77 45 31 25
12
SfoI GGC'GCC
Cuts at: 0 3882 5115
Size: 3882 1233
SmaI CCC'GGG
Cuts at: 0 557 4023 5115
Size: 557 3466 1092
Fragments arranged by size:
3466 1092 557
SmlI C'TyrA_G
Cuts at: 0 1518 3163 4834 5115
Size: 1518 1645 1671 281
Fragments arranged by size:
1671 1645 1518 281
SpeI A'CTAG_T
Cuts at: 0 4813 5115
Size: 4813 302
SphI G_CATG'C
Cuts at: 0 2096 5115
Size: 2096 3019
SspI AAT'ATT
Cuts at: 0 5103 5115
Size: 5103 12
StuI AGG'CCT
Cuts at: 0 571 841 3868 5115
Size: 571 270 3027 1247
Fragments arranged by size:
3027 1247 571 270
StyI C'CwwG_G
Cuts at: 0 183 248 392 572 1442 2145 2288 2318
Size: 183 65 144 180 870 703 143 30
Cuts at: 2318 2384 3515 5115
Size: 66 1131 1600
Fragments arranged by size:
1600 1131 870 703 183 180 144 143
66 65 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1698 2801 5115
Size: 1698 1103 2314
Fragments arranged by size:
2314 1698 1103
TatI w'GTAC_w
Cuts at: 0 3044 4713 5115
Size: 3044 1669 402
Fragments arranged by size:
3044 1669 402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1521 1553 2126 2158 2185 2217 3496 3528
Size: 1521 32 573 32 27 32 1279 32
Cuts at: 3528 4017 4049 4417 4449 4954 4986 5115
Size: 489 32 368 32 505 32 129
Fragments arranged by size:
1521 1279 573 505 489 368 129 32
32 32 32 32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 3919 5115
Size: 3919 1196
XbaI T'CTAG_A
Cuts at: 0 438 3759 5115
Size: 438 3321 1356
Fragments arranged by size:
3321 1356 438
XmaI C'CCGG_G
Cuts at: 0 555 4021 5115
Size: 555 3466 1094
Fragments arranged by size:
3466 1094 555
XmnI GAAnn'nnTTC
Cuts at: 0 462 494 1604 2154 2984 5115
Size: 462 32 1110 550 830 2131
Fragments arranged by size:
2131 1110 830 550 462 32
ZraI GAC'GTC
Cuts at: 0 651 3753 5115
Size: 651 3102 1362
Fragments arranged by size:
3102 1362 651
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PacI PciI PflMI PfoI PsiI PspOMI
PstI PvuII SacI SacII SapI SfcI SfoI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AscI AsiSI AvrII BciVI BlpI BmgBI
BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I BtgZI CspCI
FseI FspAI MfeI MluI NdeI NheI NotI PasI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SrfI SwaI XcmI XhoI