Restriction map for
pF1KA1983
(Linear) (Six-base) MAPSORT of: KIAA1983.seq Check: 447 from: 1 to: 1218
KIAA1983.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 199 664 1073 1218
Size: 170 29 465 409 145
Fragments arranged by size:
465 409 170 145 29
AflIII A'CryG_T
Cuts at: 0 92 517 1218
Size: 92 425 701
Fragments arranged by size:
701 425 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 671 1218
Size: 671 547
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1218
Size: 1051 32 135
Fragments arranged by size:
1051 135 32
ApaI G_GGCC'C
Cuts at: 0 769 910 974 1218
Size: 769 141 64 244
Fragments arranged by size:
769 244 141 64
ApaLI G'TGCA_C
Cuts at: 0 303 1218
Size: 303 915
ApoI r'AATT_y
Cuts at: 0 235 1117 1218
Size: 235 882 101
Fragments arranged by size:
882 235 101
BanI G'GyrC_C
Cuts at: 0 3 927 1073 1218
Size: 3 924 146 145
Fragments arranged by size:
924 146 145 3
BanII G_rGCy'C
Cuts at: 0 197 769 850 910 974 1153 1200 1218
Size: 197 572 81 60 64 179 47 18
Fragments arranged by size:
572 197 179 81 64 60 47 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 520 1218
Size: 176 344 698
Fragments arranged by size:
698 344 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 259 293 1218
Size: 259 34 925
Fragments arranged by size:
925 259 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 340 1218
Size: 340 878
BfrBI ATG'CAT
Cuts at: 0 254 1218
Size: 254 964
BglI GCCn_nnn'nGGC
Cuts at: 0 47 488 773 845 905 1218
Size: 47 441 285 72 60 313
Fragments arranged by size:
441 313 285 72 60 47
Bme1580I G_kGCm'C
Cuts at: 0 307 441 769 910 930 974 1076 1218
Size: 307 134 328 141 20 44 102 142
Fragments arranged by size:
328 307 142 141 134 102 44 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1175 1218
Size: 968 207 43
Fragments arranged by size:
968 207 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1212 1218
Size: 1212 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 986 1218
Size: 986 232
BsaAI yAC'GTr
Cuts at: 0 95 1218
Size: 95 1123
BsaBI GATnn'nnATC
Cuts at: 0 1163 1218
Size: 1163 55
BsaWI w'CCGG_w
Cuts at: 0 371 1076 1218
Size: 371 705 142
Fragments arranged by size:
705 371 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 45 75 1218
Size: 45 30 1143
Fragments arranged by size:
1143 45 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 41 124 974 1111 1218
Size: 41 83 850 137 107
Fragments arranged by size:
850 137 107 83 41
BseYI C'CCAG_C
Cuts at: 0 45 1123 1218
Size: 45 1078 95
Fragments arranged by size:
1078 95 45
BsiHKAI G_wGCw'C
Cuts at: 0 197 307 1218
Size: 197 110 911
Fragments arranged by size:
911 197 110
Bsp1286I G_dGCh'C
Cuts at: 0 197 307 441 769 850 910 930 974
Size: 197 110 134 328 81 60 20 44
Cuts at: 974 1076 1153 1200 1218
Size: 102 77 47 18
Fragments arranged by size:
328 197 134 110 102 81 77 60
47 44 20 18
BsrDI GCAATG_nn'
Cuts at: 0 429 1044 1218
Size: 429 615 174
Fragments arranged by size:
615 429 174
BstAPI GCAn_nnn'nTGC
Cuts at: 0 633 1218
Size: 633 585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1146 1218
Size: 1146 72
Bsu36I CC'TnA_GG
Cuts at: 0 1112 1218
Size: 1112 106
BtgI C'CryG_G
Cuts at: 0 841 860 1139 1218
Size: 841 19 279 79
Fragments arranged by size:
841 279 79 19
BtsI GCAGTG_nn'
Cuts at: 0 307 1218
Size: 307 911
EaeI y'GGCC_r
Cuts at: 0 318 552 1218
Size: 318 234 666
Fragments arranged by size:
666 318 234
EarI CTCTTCn'nnn_
Cuts at: 0 930 1092 1218
Size: 930 162 126
Fragments arranged by size:
930 162 126
EcoICRI GAG'CTC
Cuts at: 0 195 1218
Size: 195 1023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 199 664 968 1073 1175 1218
Size: 170 29 465 304 105 102 43
Fragments arranged by size:
465 304 170 105 102 43 29
EcoO109I rG'GnC_Cy
Cuts at: 0 739 765 766 820 856 906 907 988
Size: 739 26 1 54 36 50 1 81
Cuts at: 988 1218
Size: 230
Fragments arranged by size:
739 230 81 54 50 36 26 1
1
HaeII r_GCGC'y
Cuts at: 0 967 1218
Size: 967 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 45 77 239 271 1135 1167 1218
Size: 45 32 162 32 864 32 51
Fragments arranged by size:
864 162 51 45 32 32 32
MscI TGG'CCA
Cuts at: 0 554 1218
Size: 554 664
MspA1I CmG'CkG
Cuts at: 0 45 66 473 642 679 1218
Size: 45 21 407 169 37 539
Fragments arranged by size:
539 407 169 45 37 21
NcoI C'CATG_G
Cuts at: 0 860 1139 1218
Size: 860 279 79
Fragments arranged by size:
860 279 79
NruI TCG'CGA
Cuts at: 0 157 1218
Size: 157 1061
NsiI A_TGCA'T
Cuts at: 0 256 1218
Size: 256 962
NspI r_CATG'y
Cuts at: 0 206 521 605 1218
Size: 206 315 84 613
Fragments arranged by size:
613 315 206 84
PciI A'CATG_T
Cuts at: 0 517 1218
Size: 517 701
PfoI T'CCnGG_A
Cuts at: 0 342 497 735 984 1218
Size: 342 155 238 249 234
Fragments arranged by size:
342 249 238 234 155
PmlI CAC'GTG
Cuts at: 0 95 1218
Size: 95 1123
PpuMI rG'GwC_Cy
Cuts at: 0 739 856 988 1218
Size: 739 117 132 230
Fragments arranged by size:
739 230 132 117
PspOMI G'GGCC_C
Cuts at: 0 765 906 970 1218
Size: 765 141 64 248
Fragments arranged by size:
765 248 141 64
PstI C_TGCA'G
Cuts at: 0 641 1058 1218
Size: 641 417 160
Fragments arranged by size:
641 417 160
PvuII CAG'CTG
Cuts at: 0 45 642 679 1218
Size: 45 597 37 539
Fragments arranged by size:
597 539 45 37
SacI G_AGCT'C
Cuts at: 0 197 1218
Size: 197 1021
SanDI GG'GwC_CC
Cuts at: 0 856 1218
Size: 856 362
SfcI C'TryA_G
Cuts at: 0 102 207 637 1054 1218
Size: 102 105 430 417 164
Fragments arranged by size:
430 417 164 105 102
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 905 1218
Size: 905 313
SmlI C'TyrA_G
Cuts at: 0 1191 1218
Size: 1191 27
StyI C'CwwG_G
Cuts at: 0 458 860 1139 1218
Size: 458 402 279 79
Fragments arranged by size:
458 402 279 79
TatI w'GTAC_w
Cuts at: 0 331 1218
Size: 331 887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 420 452 717 749 1218
Size: 420 32 265 32 469
Fragments arranged by size:
469 420 265 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 1218
Size: 42 1176
Enzymes that do cut:
AcuI AflIII AlfI AloI ApaI ApaLI ApoI BanI
BanII BbsI BcgI BciVI BfrBI BglI Bme1580I BpmI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BsrDI BstAPI BstXI Bsu36I BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I MscI
MspA1I NcoI NruI NsiI NspI PciI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SfcI SfiI
SmlI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlwNI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BclI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaHI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI PacI PasI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI