Restriction map for
pF1KSDA1971
(Linear) (Six-base) MAPSORT of: KIAA1971.seq Check: 4829 from: 1 to: 2427
KIAA1971.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2085 2427
Size: 2085 342
AclI AA'CG_TT
Cuts at: 0 656 2427
Size: 656 1771
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 884 1000 1520 2278 2427
Size: 863 21 116 520 758 149
Fragments arranged by size:
863 758 520 149 116 21
AfeI AGC'GCT
Cuts at: 0 307 2427
Size: 307 2120
AflII C'TTAA_G
Cuts at: 0 2148 2427
Size: 2148 279
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 103 135 2427
Size: 103 32 2292
Fragments arranged by size:
2292 103 32
AlwNI CAG_nnn'CTG
Cuts at: 0 432 1401 1424 1684 2427
Size: 432 969 23 260 743
Fragments arranged by size:
969 743 432 260 23
ApaI G_GGCC'C
Cuts at: 0 177 188 2427
Size: 177 11 2239
Fragments arranged by size:
2239 177 11
ApoI r'AATT_y
Cuts at: 0 1091 1172 1198 1362 2427
Size: 1091 81 26 164 1065
Fragments arranged by size:
1091 1065 164 81 26
AseI AT'TA_AT
Cuts at: 0 649 2427
Size: 649 1778
AvaI C'yCGr_G
Cuts at: 0 67 187 193 370 1031 2427
Size: 67 120 6 177 661 1396
Fragments arranged by size:
1396 661 177 120 67 6
AvrII C'CTAG_G
Cuts at: 0 804 2427
Size: 804 1623
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 126 159 1506 1539 2427
Size: 126 33 1347 33 888
Fragments arranged by size:
1347 888 126 33 33
BanI G'GyrC_C
Cuts at: 0 36 74 484 2427
Size: 36 38 410 1943
Fragments arranged by size:
1943 410 38 36
BanII G_rGCy'C
Cuts at: 0 68 177 188 194 200 260 371 2034
Size: 68 109 11 6 6 60 111 1663
Cuts at: 2034 2166 2427
Size: 132 261
Fragments arranged by size:
1663 261 132 111 109 68 60 11
6 6
BbeI G_GCGC'C
Cuts at: 0 488 2427
Size: 488 1939
BbvCI CC'TCA_GC
Cuts at: 0 515 2427
Size: 515 1912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2059 2093 2427
Size: 2059 34 334
Fragments arranged by size:
2059 334 34
BclI T'GATC_A
Cuts at: 0 1875 2427
Size: 1875 552
BglI GCCn_nnn'nGGC
Cuts at: 0 29 72 2427
Size: 29 43 2355
Fragments arranged by size:
2355 43 29
BglII A'GATC_T
Cuts at: 0 1110 2427
Size: 1110 1317
BlpI GC'TnA_GC
Cuts at: 0 2081 2427
Size: 2081 346
Bme1580I G_kGCm'C
Cuts at: 0 177 188 2427
Size: 177 11 2239
Fragments arranged by size:
2239 177 11
BmgBI CAC'GTC
Cuts at: 0 339 2056 2427
Size: 339 1717 371
Fragments arranged by size:
1717 371 339
BmtI G_CTAG'C
Cuts at: 0 1030 2427
Size: 1030 1397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1459 2185 2427
Size: 1459 726 242
Fragments arranged by size:
1459 726 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 322 850 1423 2018 2338 2427
Size: 46 276 528 573 595 320 89
Fragments arranged by size:
595 573 528 320 276 89 46
Bpu10I CC'TnA_GC
Cuts at: 0 515 2427
Size: 515 1912
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1982 2350 2427
Size: 1982 368 77
Fragments arranged by size:
1982 368 77
BsaAI yAC'GTr
Cuts at: 0 2092 2427
Size: 2092 335
BsaBI GATnn'nnATC
Cuts at: 0 1626 2427
Size: 1626 801
BsaHI Gr'CG_yC
Cuts at: 0 485 2427
Size: 485 1942
BsaWI w'CCGG_w
Cuts at: 0 1445 2171 2427
Size: 1445 726 256
Fragments arranged by size:
1445 726 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 2427
Size: 103 30 2294
Fragments arranged by size:
2294 103 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1582 1913 1916 1919 1961 2156 2349 2401
Size: 1582 331 3 3 42 195 193 52
Cuts at: 2401 2427
Size: 26
Fragments arranged by size:
1582 331 195 193 52 42 26 3
3
BseYI C'CCAG_C
Cuts at: 0 32 230 1715 2290 2427
Size: 32 198 1485 575 137
Fragments arranged by size:
1485 575 198 137 32
BsiEI CG_ry'CG
Cuts at: 0 141 251 470 530 2427
Size: 141 110 219 60 1897
Fragments arranged by size:
1897 219 141 110 60
BsiHKAI G_wGCw'C
Cuts at: 0 1983 2166 2225 2427
Size: 1983 183 59 202
Fragments arranged by size:
1983 202 183 59
BsmI GAATG_Cn'
Cuts at: 0 941 2427
Size: 941 1486
BsmBI CGTCTCn'nnnn_
Cuts at: 0 222 882 2427
Size: 222 660 1545
Fragments arranged by size:
1545 660 222
Bsp1286I G_dGCh'C
Cuts at: 0 68 177 188 194 200 260 371 1983
Size: 68 109 11 6 6 60 111 1612
Cuts at: 1983 2034 2166 2225 2427
Size: 51 132 59 202
Fragments arranged by size:
1612 202 132 111 109 68 60 59
51 11 6 6
BspEI T'CCGG_A
Cuts at: 0 1445 2171 2427
Size: 1445 726 256
Fragments arranged by size:
1445 726 256
BspHI T'CATG_A
Cuts at: 0 1049 2427
Size: 1049 1378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1706 2085 2427
Size: 1706 379 342
Fragments arranged by size:
1706 379 342
BsrBI CCG'CTC
Cuts at: 0 561 2427
Size: 561 1866
BsrFI r'CCGG_y
Cuts at: 0 39 77 235 272 2187 2427
Size: 39 38 158 37 1915 240
Fragments arranged by size:
1915 240 158 39 38 37
BssHII G'CGCG_C
Cuts at: 0 266 399 570 572 2427
Size: 266 133 171 2 1855
Fragments arranged by size:
1855 266 171 133 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 2427
Size: 81 2346
BstEII G'GTnAC_C
Cuts at: 0 693 2427
Size: 693 1734
BstXI CCAn_nnnn'nTGG
Cuts at: 0 639 2422 2427
Size: 639 1783 5
Fragments arranged by size:
1783 639 5
BstYI r'GATC_y
Cuts at: 0 1110 1421 2122 2427
Size: 1110 311 701 305
Fragments arranged by size:
1110 701 311 305
Bsu36I CC'TnA_GG
Cuts at: 0 206 2427
Size: 206 2221
BtgI C'CryG_G
Cuts at: 0 251 261 316 638 698 2427
Size: 251 10 55 322 60 1729
Fragments arranged by size:
1729 322 251 60 55 10
BtsI GCAGTG_nn'
Cuts at: 0 1396 1909 2427
Size: 1396 513 518
Fragments arranged by size:
1396 518 513
DraI TTT'AAA
Cuts at: 0 1073 1635 2427
Size: 1073 562 792
Fragments arranged by size:
1073 792 562
DraIII CAC_nnn'GTG
Cuts at: 0 1746 2427
Size: 1746 681
EaeI y'GGCC_r
Cuts at: 0 248 292 391 467 527 2412 2427
Size: 248 44 99 76 60 1885 15
Fragments arranged by size:
1885 248 99 76 60 44 15
EagI C'GGCC_G
Cuts at: 0 248 467 527 2427
Size: 248 219 60 1900
Fragments arranged by size:
1900 248 219 60
EarI CTCTTCn'nnn_
Cuts at: 0 61 511 958 1559 2427
Size: 61 450 447 601 868
Fragments arranged by size:
868 601 450 447 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 604 2427
Size: 604 1823
EcoICRI GAG'CTC
Cuts at: 0 2164 2427
Size: 2164 263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 322 850 863 884 1000 1423 1520
Size: 46 276 528 13 21 116 423 97
Cuts at: 1520 2018 2278 2338 2427
Size: 498 260 60 89
Fragments arranged by size:
528 498 423 276 260 116 97 89
60 46 21 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2148 2427
Size: 2148 279
EcoO109I rG'GnC_Cy
Cuts at: 0 51 173 184 353 406 808 2406 2427
Size: 51 122 11 169 53 402 1598 21
Fragments arranged by size:
1598 402 169 122 53 51 21 11
EcoRV GAT'ATC
Cuts at: 0 891 2427
Size: 891 1536
FseI GG_CCGG'CC
Cuts at: 0 239 2427
Size: 239 2188
HaeII r_GCGC'y
Cuts at: 0 289 309 385 424 488 2427
Size: 289 20 76 39 64 1939
Fragments arranged by size:
1939 289 76 64 39 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 957 989 1095 1127 1891 1923 2427
Size: 957 32 106 32 764 32 504
Fragments arranged by size:
957 764 504 106 32 32 32
HindIII A'AGCT_T
Cuts at: 0 769 2427
Size: 769 1658
KasI G'GCGC_C
Cuts at: 0 484 2427
Size: 484 1943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1223 1641 2427
Size: 986 237 418 786
Fragments arranged by size:
986 786 418 237
MscI TGG'CCA
Cuts at: 0 2414 2427
Size: 2414 13
MspA1I CmG'CkG
Cuts at: 0 156 230 253 263 318 444 1401 2427
Size: 156 74 23 10 55 126 957 1026
Fragments arranged by size:
1026 957 156 126 74 55 23 10
NaeI GCC'GGC
Cuts at: 0 237 2189 2427
Size: 237 1952 238
Fragments arranged by size:
1952 238 237
NarI GG'CG_CC
Cuts at: 0 485 2427
Size: 485 1942
NcoI C'CATG_G
Cuts at: 0 638 2427
Size: 638 1789
NgoMIV G'CCGG_C
Cuts at: 0 235 2187 2427
Size: 235 1952 240
Fragments arranged by size:
1952 240 235
NheI G'CTAG_C
Cuts at: 0 1026 2427
Size: 1026 1401
PflMI CCAn_nnn'nTGG
Cuts at: 0 690 1401 2427
Size: 690 711 1026
Fragments arranged by size:
1026 711 690
PfoI T'CCnGG_A
Cuts at: 0 44 554 2118 2427
Size: 44 510 1564 309
Fragments arranged by size:
1564 510 309 44
PmlI CAC'GTG
Cuts at: 0 2092 2427
Size: 2092 335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 2427
Size: 103 32 2292
Fragments arranged by size:
2292 103 32
PpuMI rG'GwC_Cy
Cuts at: 0 406 808 2406 2427
Size: 406 402 1598 21
Fragments arranged by size:
1598 406 402 21
PshAI GACnn'nnGTC
Cuts at: 0 806 2427
Size: 806 1621
PsiI TTA'TAA
Cuts at: 0 1264 2427
Size: 1264 1163
PspOMI G'GGCC_C
Cuts at: 0 173 184 2427
Size: 173 11 2243
Fragments arranged by size:
2243 173 11
PstI C_TGCA'G
Cuts at: 0 428 1352 2427
Size: 428 924 1075
Fragments arranged by size:
1075 924 428
PvuII CAG'CTG
Cuts at: 0 230 444 1401 2427
Size: 230 214 957 1026
Fragments arranged by size:
1026 957 230 214
RsrII CG'GwC_CG
Cuts at: 0 42 2427
Size: 42 2385
SacI G_AGCT'C
Cuts at: 0 2166 2427
Size: 2166 261
SacII CC_GC'GG
Cuts at: 0 254 264 319 2427
Size: 254 10 55 2108
Fragments arranged by size:
2108 254 55 10
SanDI GG'GwC_CC
Cuts at: 0 406 2427
Size: 406 2021
ScaI AGT'ACT
Cuts at: 0 721 2427
Size: 721 1706
SfcI C'TryA_G
Cuts at: 0 424 1348 2427
Size: 424 924 1079
Fragments arranged by size:
1079 924 424
SfoI GGC'GCC
Cuts at: 0 486 2427
Size: 486 1941
SmlI C'TyrA_G
Cuts at: 0 1031 1997 2148 2329 2427
Size: 1031 966 151 181 98
Fragments arranged by size:
1031 966 181 151 98
StyI C'CwwG_G
Cuts at: 0 638 804 2099 2427
Size: 638 166 1295 328
Fragments arranged by size:
1295 638 328 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 2427
Size: 24 2403
TatI w'GTAC_w
Cuts at: 0 719 2427
Size: 719 1708
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 981 1013 2427
Size: 981 32 1414
Fragments arranged by size:
1414 981 32
XhoI C'TCGA_G
Cuts at: 0 1031 2427
Size: 1031 1396
XmnI GAAnn'nnTTC
Cuts at: 0 1013 1112 2427
Size: 1013 99 1315
Fragments arranged by size:
1315 1013 99
Enzymes that do cut:
AarI AclI AcuI AfeI AflII AloI AlwNI ApaI
ApoI AseI AvaI AvrII BaeI BanI BanII BbeI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FseI HaeII Hin4I HindIII KasI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NheI PflMI PfoI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII
RsrII SacI SacII SanDI ScaI SfcI SfoI SmlI
StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflIII AgeI AhdI AleI AlfI
ApaLI AscI AsiSI BamHI BbsI BciVI BfrBI BmrI
BsaI BsgI BsiWI BsrDI BsrGI BssSI BstBI BstZ17I
BtgZI ClaI CspCI DrdI EcoRI FalI FspI FspAI
HincII HpaI KpnI MfeI MluI MslI NdeI NotI
NruI NsiI NspI PacI PasI PciI PmeI PspXI
PsrI PvuI SalI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XmaI ZraI