Restriction map for
pF1KA1966
(Linear) (Six-base) MAPSORT of: KIAA1966.seq Check: 5874 from: 1 to: 2181
KIAA1966.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AatII G_ACGT'C
Cuts at: 0 1566 2181
Size: 1566 615
AccI GT'mk_AC
Cuts at: 0 1605 2028 2181
Size: 1605 423 153
Fragments arranged by size:
1605 423 153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 548 1232 2181
Size: 83 465 684 949
Fragments arranged by size:
949 684 465 83
ApoI r'AATT_y
Cuts at: 0 341 1222 1783 2181
Size: 341 881 561 398
Fragments arranged by size:
881 561 398 341
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 50 83 2181
Size: 50 33 2098
Fragments arranged by size:
2098 50 33
BamHI G'GATC_C
Cuts at: 0 414 1695 2181
Size: 414 1281 486
Fragments arranged by size:
1281 486 414
BanII G_rGCy'C
Cuts at: 0 226 2181
Size: 226 1955
BbsI GAAGACnn'nnnn_
Cuts at: 0 497 1459 2181
Size: 497 962 722
Fragments arranged by size:
962 722 497
BfrBI ATG'CAT
Cuts at: 0 1539 2181
Size: 1539 642
BglII A'GATC_T
Cuts at: 0 304 856 1174 2181
Size: 304 552 318 1007
Fragments arranged by size:
1007 552 318 304
BmgBI CAC'GTC
Cuts at: 0 484 1423 2107 2181
Size: 484 939 684 74
Fragments arranged by size:
939 684 484 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 1290 2181
Size: 1290 891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 2181
Size: 348 1833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1874 2181
Size: 1874 307
BsaI GGTCTCn'nnnn_
Cuts at: 0 758 1671 2036 2054 2144 2181
Size: 758 913 365 18 90 37
Fragments arranged by size:
913 758 365 90 37 18
BsaBI GATnn'nnATC
Cuts at: 0 332 2181
Size: 332 1849
BsaHI Gr'CG_yC
Cuts at: 0 1563 1602 2181
Size: 1563 39 579
Fragments arranged by size:
1563 579 39
BsaWI w'CCGG_w
Cuts at: 0 404 2181
Size: 404 1777
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 616 703 706 709 718 721 724
Size: 34 582 87 3 3 9 3 3
Cuts at: 724 733 736 739 742 745 790 1877 2181
Size: 9 3 3 3 3 45 1087 304
Fragments arranged by size:
1087 582 304 87 45 34 9 9
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1314 1457 2181
Size: 1314 143 724
Fragments arranged by size:
1314 724 143
BsiHKAI G_wGCw'C
Cuts at: 0 226 2181
Size: 226 1955
BsmI GAATG_Cn'
Cuts at: 0 1539 1541 1826 2181
Size: 1539 2 285 355
Fragments arranged by size:
1539 355 285 2
Bsp1286I G_dGCh'C
Cuts at: 0 226 2181
Size: 226 1955
BspEI T'CCGG_A
Cuts at: 0 404 2181
Size: 404 1777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 961 2181
Size: 961 1220
BssSI C'ACGA_G
Cuts at: 0 1556 2181
Size: 1556 625
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1821 2181
Size: 1821 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 553 1811 2181
Size: 553 1258 370
Fragments arranged by size:
1258 553 370
BstYI r'GATC_y
Cuts at: 0 304 414 493 856 1174 1267 1695 2181
Size: 304 110 79 363 318 93 428 486
Fragments arranged by size:
486 428 363 318 304 110 93 79
BtsI GCAGTG_nn'
Cuts at: 0 567 2181
Size: 567 1614
DraI TTT'AAA
Cuts at: 0 1329 1691 1754 2181
Size: 1329 362 63 427
Fragments arranged by size:
1329 427 362 63
DraIII CAC_nnn'GTG
Cuts at: 0 1284 2181
Size: 1284 897
EaeI y'GGCC_r
Cuts at: 0 2016 2181
Size: 2016 165
EarI CTCTTCn'nnn_
Cuts at: 0 662 695 710 898 2181
Size: 662 33 15 188 1283
Fragments arranged by size:
1283 662 188 33 15
EcoICRI GAG'CTC
Cuts at: 0 224 2181
Size: 224 1957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 348 548 1232 2181
Size: 83 265 200 684 949
Fragments arranged by size:
949 684 265 200 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1814 1832 1907 2181
Size: 1814 18 75 274
Fragments arranged by size:
1814 274 75 18
EcoO109I rG'GnC_Cy
Cuts at: 0 1760 2181
Size: 1760 421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1262 2181
Size: 1262 919
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1058 1087 1090 1119 1568 1600
Size: 982 32 44 29 3 29 449 32
Cuts at: 1600 1641 1673 2181
Size: 41 32 508
Fragments arranged by size:
982 508 449 44 41 32 32 32
29 29 3
HincII GTy'rAC
Cuts at: 0 1606 2181
Size: 1606 575
MfeI C'AATT_G
Cuts at: 0 926 2181
Size: 926 1255
MslI CAynn'nnrTG
Cuts at: 0 1097 1964 2181
Size: 1097 867 217
Fragments arranged by size:
1097 867 217
NdeI CA'TA_TG
Cuts at: 0 966 2181
Size: 966 1215
NsiI A_TGCA'T
Cuts at: 0 1541 2181
Size: 1541 640
PflMI CCAn_nnn'nTGG
Cuts at: 0 1391 1713 2181
Size: 1391 322 468
Fragments arranged by size:
1391 468 322
PfoI T'CCnGG_A
Cuts at: 0 1586 2181
Size: 1586 595
PpuMI rG'GwC_Cy
Cuts at: 0 1760 2181
Size: 1760 421
PsiI TTA'TAA
Cuts at: 0 377 2181
Size: 377 1804
SacI G_AGCT'C
Cuts at: 0 226 2181
Size: 226 1955
SalI G'TCGA_C
Cuts at: 0 1604 2181
Size: 1604 577
SmlI C'TyrA_G
Cuts at: 0 1889 2181
Size: 1889 292
SspI AAT'ATT
Cuts at: 0 1199 2181
Size: 1199 982
StyI C'CwwG_G
Cuts at: 0 1390 1645 1810 2181
Size: 1390 255 165 371
Fragments arranged by size:
1390 371 255 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1272 2168 2181
Size: 1272 896 13
Fragments arranged by size:
1272 896 13
TatI w'GTAC_w
Cuts at: 0 256 1956 2181
Size: 256 1700 225
Fragments arranged by size:
1700 256 225
XbaI T'CTAG_A
Cuts at: 0 324 2181
Size: 324 1857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1808 2181
Size: 1808 373
XmnI GAAnn'nnTTC
Cuts at: 0 1539 2181
Size: 1539 642
ZraI GAC'GTC
Cuts at: 0 1564 2181
Size: 1564 617
Enzymes that do cut:
AatII AccI AcuI ApoI BaeI BamHI BanII BbsI
BfrBI BglII BmgBI BmrI BpmI BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspMI BssSI BstAPI BstXI BstYI BtsI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HincII MfeI MslI NdeI NsiI PflMI PfoI PpuMI
PsiI SacI SalI SmlI SspI StyI TaqII TatI
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BanI BbeI BbvCI BcgI BciVI
BclI BglI BlpI Bme1580I BmtI BplI Bpu10I BsaAI
BsaXI BsgI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DrdI EagI EciI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XhoI XmaI