Restriction map for
pF1KA1962
(Linear) (Six-base) MAPSORT of: KIAA1962.seq Check: 2280 from: 1 to: 2232
KIAA1962.orf
With 182 enzymes: SgfI *
November 10, 2008 09:34 ..
AccI GT'mk_AC
Cuts at: 0 968 2232
Size: 968 1264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 999 1097 1662 1998 2232
Size: 926 73 98 565 336 234
Fragments arranged by size:
926 565 336 234 98 73
AflIII A'CryG_T
Cuts at: 0 1654 2232
Size: 1654 578
AlwNI CAG_nnn'CTG
Cuts at: 0 240 300 2232
Size: 240 60 1932
Fragments arranged by size:
1932 240 60
ApoI r'AATT_y
Cuts at: 0 619 837 895 1376 1544 1628 1712 1770
Size: 619 218 58 481 168 84 84 58
Cuts at: 1770 1796 1964 2048 2132 2232
Size: 26 168 84 84 100
Fragments arranged by size:
619 481 218 168 168 100 84 84
84 84 58 58 26
BanI G'GyrC_C
Cuts at: 0 752 2232
Size: 752 1480
BanII G_rGCy'C
Cuts at: 0 227 2232
Size: 227 2005
BbsI GAAGACnn'nnnn_
Cuts at: 0 2182 2232
Size: 2182 50
BclI T'GATC_A
Cuts at: 0 768 2232
Size: 768 1464
Bme1580I G_kGCm'C
Cuts at: 0 17 2232
Size: 17 2215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 468 2045 2232
Size: 468 1577 187
Fragments arranged by size:
1577 468 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1406 1574 1658 1742 1826 2232
Size: 580 826 168 84 84 84 406
Fragments arranged by size:
826 580 406 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 993 2232
Size: 407 586 1239
Fragments arranged by size:
1239 586 407
BsaI GGTCTCn'nnnn_
Cuts at: 0 237 1581 2232
Size: 237 1344 651
Fragments arranged by size:
1344 651 237
BsaBI GATnn'nnATC
Cuts at: 0 767 2232
Size: 767 1465
BsaWI w'CCGG_w
Cuts at: 0 2140 2232
Size: 2140 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 867 897 2232
Size: 867 30 1335
Fragments arranged by size:
1335 867 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 557 829 2232
Size: 460 97 272 1403
Fragments arranged by size:
1403 460 272 97
BseYI C'CCAG_C
Cuts at: 0 659 791 2232
Size: 659 132 1441
Fragments arranged by size:
1441 659 132
BsiHKAI G_wGCw'C
Cuts at: 0 227 1524 2042 2232
Size: 227 1297 518 190
Fragments arranged by size:
1297 518 227 190
Bsp1286I G_dGCh'C
Cuts at: 0 17 227 1524 2042 2232
Size: 17 210 1297 518 190
Fragments arranged by size:
1297 518 210 190 17
BstEII G'GTnAC_C
Cuts at: 0 369 2232
Size: 369 1863
BstYI r'GATC_y
Cuts at: 0 411 2232
Size: 411 1821
Bsu36I CC'TnA_GG
Cuts at: 0 607 757 2232
Size: 607 150 1475
Fragments arranged by size:
1475 607 150
BtgI C'CryG_G
Cuts at: 0 682 2232
Size: 682 1550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1424 2232
Size: 1424 808
BtsI GCAGTG_nn'
Cuts at: 0 1942 2232
Size: 1942 290
DraI TTT'AAA
Cuts at: 0 2184 2232
Size: 2184 48
EarI CTCTTCn'nnn_
Cuts at: 0 843 1116 2232
Size: 843 273 1116
Fragments arranged by size:
1116 843 273
EcoICRI GAG'CTC
Cuts at: 0 225 2232
Size: 225 2007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 926 999 1097 1406 1574 1658 1662
Size: 580 346 73 98 309 168 84 4
Cuts at: 1662 1742 1826 1998 2232
Size: 80 84 172 234
Fragments arranged by size:
580 346 309 234 172 168 98 84
84 80 73 4
EcoO109I rG'GnC_Cy
Cuts at: 0 81 1163 2232
Size: 81 1082 1069
Fragments arranged by size:
1082 1069 81
EcoRI G'AATT_C
Cuts at: 0 1376 1544 1628 1712 1770 1796 1964 2048
Size: 1376 168 84 84 58 26 168 84
Cuts at: 2048 2232
Size: 184
Fragments arranged by size:
1376 184 168 168 84 84 84 58
26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1029 2232
Size: 1029 1203
HaeII r_GCGC'y
Cuts at: 0 1355 2232
Size: 1355 877
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 403 435 1423 1455 1591 1623
Size: 130 32 241 32 988 32 136 32
Cuts at: 1623 1857 1889 2232
Size: 234 32 343
Fragments arranged by size:
988 343 241 234 136 130 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 214 2232
Size: 214 2018
HindIII A'AGCT_T
Cuts at: 0 1421 2232
Size: 1421 811
MfeI C'AATT_G
Cuts at: 0 1662 2232
Size: 1662 570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 2232
Size: 1228 1004
MslI CAynn'nnrTG
Cuts at: 0 1821 1973 2232
Size: 1821 152 259
Fragments arranged by size:
1821 259 152
NcoI C'CATG_G
Cuts at: 0 682 2232
Size: 682 1550
NspI r_CATG'y
Cuts at: 0 1658 2232
Size: 1658 574
PacI TTA_AT'TAA
Cuts at: 0 953 2232
Size: 953 1279
PciI A'CATG_T
Cuts at: 0 1654 2232
Size: 1654 578
PpuMI rG'GwC_Cy
Cuts at: 0 81 1163 2232
Size: 81 1082 1069
Fragments arranged by size:
1082 1069 81
PsiI TTA'TAA
Cuts at: 0 1568 1988 2232
Size: 1568 420 244
Fragments arranged by size:
1568 420 244
PstI C_TGCA'G
Cuts at: 0 2229 2232
Size: 2229 3
SacI G_AGCT'C
Cuts at: 0 227 2232
Size: 227 2005
SfcI C'TryA_G
Cuts at: 0 8 1347 2225 2232
Size: 8 1339 878 7
Fragments arranged by size:
1339 878 8 7
SmlI C'TyrA_G
Cuts at: 0 422 972 2232
Size: 422 550 1260
Fragments arranged by size:
1260 550 422
SspI AAT'ATT
Cuts at: 0 799 2232
Size: 799 1433
StuI AGG'CCT
Cuts at: 0 2198 2232
Size: 2198 34
StyI C'CwwG_G
Cuts at: 0 682 2232
Size: 682 1550
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 465 497 2232
Size: 465 32 1735
Fragments arranged by size:
1735 465 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 799 2232
Size: 799 1433
XmnI GAAnn'nnTTC
Cuts at: 0 1012 1480 1507 1927 2011 2232
Size: 1012 468 27 420 84 221
Fragments arranged by size:
1012 468 420 221 84 27
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI BanI BanII BbsI
BclI Bme1580I BplI BpmI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII MfeI MmeI
MslI NcoI NspI PacI PciI PpuMI PsiI PstI
SacI SfcI SmlI SspI StuI StyI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaAI BsaHI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoNI EcoRV FseI FspI FspAI
HpaI KasI KpnI MluI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI ZraI