Restriction map for
pF1KSDA1960
(Linear) (Six-base) MAPSORT of: KIAA1960.seq Check: 6020 from: 1 to: 2556
KIAA1960.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AatII G_ACGT'C
Cuts at: 0 469 2556
Size: 469 2087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 876 905 1969 2305 2422 2556
Size: 418 458 29 1064 336 117 134
Fragments arranged by size:
1064 458 418 336 134 117 29
AfeI AGC'GCT
Cuts at: 0 918 2556
Size: 918 1638
AgeI A'CCGG_T
Cuts at: 0 2343 2556
Size: 2343 213
AhdI GACnn_n'nnGTC
Cuts at: 0 867 2556
Size: 867 1689
AleI CACnn'nnGTG
Cuts at: 0 2031 2556
Size: 2031 525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 514 1371 2556
Size: 514 857 1185
Fragments arranged by size:
1185 857 514
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 847 879 2556
Size: 454 32 361 32 1677
Fragments arranged by size:
1677 454 361 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 622 1339 1644 1839 2094 2556
Size: 622 717 305 195 255 462
Fragments arranged by size:
717 622 462 305 255 195
ApaI G_GGCC'C
Cuts at: 0 214 764 1232 2556
Size: 214 550 468 1324
Fragments arranged by size:
1324 550 468 214
AvaI C'yCGr_G
Cuts at: 0 127 207 300 309 408 573 602 763
Size: 127 80 93 9 99 165 29 161
Cuts at: 763 829 1624 1850 2192 2556
Size: 66 795 226 342 364
Fragments arranged by size:
795 364 342 226 165 161 127 99
93 80 66 29 9
BanI G'GyrC_C
Cuts at: 0 2042 2556
Size: 2042 514
BanII G_rGCy'C
Cuts at: 0 214 488 609 764 770 1232 1919 2556
Size: 214 274 121 155 6 462 687 637
Fragments arranged by size:
687 637 462 274 214 155 121 6
BbeI G_GCGC'C
Cuts at: 0 2046 2556
Size: 2046 510
BciVI GTATCCnnnnn_n'
Cuts at: 0 1076 2556
Size: 1076 1480
BglI GCCn_nnn'nGGC
Cuts at: 0 37 412 575 1140 1608 1908 2197 2377
Size: 37 375 163 565 468 300 289 180
Cuts at: 2377 2398 2556
Size: 21 158
Fragments arranged by size:
565 468 375 300 289 180 163 158
37 21
BlpI GC'TnA_GC
Cuts at: 0 565 2556
Size: 565 1991
Bme1580I G_kGCm'C
Cuts at: 0 214 764 1232 1582 2085 2556
Size: 214 550 468 350 503 471
Fragments arranged by size:
550 503 471 468 350 214
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 1424 2556
Size: 186 1238 1132
Fragments arranged by size:
1238 1132 186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 756 2244 2556
Size: 756 1488 312
Fragments arranged by size:
1488 756 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 1529 1799 2077 2116 2507 2556
Size: 726 803 270 278 39 391 49
Fragments arranged by size:
803 726 391 278 270 49 39
Bpu10I CC'TnA_GC
Cuts at: 0 1275 2556
Size: 1275 1281
BsaI GGTCTCn'nnnn_
Cuts at: 0 1114 1119 2556
Size: 1114 5 1437
Fragments arranged by size:
1437 1114 5
BsaAI yAC'GTr
Cuts at: 0 186 2295 2556
Size: 186 2109 261
Fragments arranged by size:
2109 261 186
BsaHI Gr'CG_yC
Cuts at: 0 466 2043 2273 2453 2556
Size: 466 1577 230 180 103
Fragments arranged by size:
1577 466 230 180 103
BsaWI w'CCGG_w
Cuts at: 0 2343 2556
Size: 2343 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 16 46 454 484 538 568 847 877
Size: 16 30 408 30 54 30 279 30
Cuts at: 877 2234 2264 2505 2535 2556
Size: 1357 30 241 30 21
Fragments arranged by size:
1357 408 279 241 54 30 30 30
30 30 30 21 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1486 1642 1798 1838 1870 2281 2365 2556
Size: 1486 156 156 40 32 411 84 191
Fragments arranged by size:
1486 411 191 156 156 84 40 32
BseYI C'CCAG_C
Cuts at: 0 394 525 1233 1509 1685 1690 1832 2154
Size: 394 131 708 276 176 5 142 322
Cuts at: 2154 2402 2491 2556
Size: 248 89 65
Fragments arranged by size:
708 394 322 276 248 176 142 131
89 65 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1907 2556
Size: 1907 649
BsiEI CG_ry'CG
Cuts at: 0 27 359 630 2556
Size: 27 332 271 1926
Fragments arranged by size:
1926 332 271 27
BsiHKAI G_wGCw'C
Cuts at: 0 458 488 2294 2340 2556
Size: 458 30 1806 46 216
Fragments arranged by size:
1806 458 216 46 30
BsmI GAATG_Cn'
Cuts at: 0 727 2556
Size: 727 1829
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 826 2492 2556
Size: 457 369 1666 64
Fragments arranged by size:
1666 457 369 64
Bsp1286I G_dGCh'C
Cuts at: 0 214 458 488 609 764 770 1232 1582
Size: 214 244 30 121 155 6 462 350
Cuts at: 1582 1919 2085 2294 2340 2556
Size: 337 166 209 46 216
Fragments arranged by size:
462 350 337 244 216 214 209 166
155 121 46 30 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1132 2556
Size: 1132 1424
BsrBI CCG'CTC
Cuts at: 0 1320 2010 2100 2310 2556
Size: 1320 690 90 210 246
Fragments arranged by size:
1320 690 246 210 90
BsrDI GCAATG_nn'
Cuts at: 0 1542 1760 2556
Size: 1542 218 796
Fragments arranged by size:
1542 796 218
BsrFI r'CCGG_y
Cuts at: 0 402 591 625 711 1842 2108 2343 2556
Size: 402 189 34 86 1131 266 235 213
Fragments arranged by size:
1131 402 266 235 213 189 86 34
BssHII G'CGCG_C
Cuts at: 0 81 440 595 2170 2556
Size: 81 359 155 1575 386
Fragments arranged by size:
1575 386 359 155 81
BssSI C'ACGA_G
Cuts at: 0 811 2556
Size: 811 1745
BstXI CCAn_nnnn'nTGG
Cuts at: 0 858 1264 1288 1906 2556
Size: 858 406 24 618 650
Fragments arranged by size:
858 650 618 406 24
BstYI r'GATC_y
Cuts at: 0 1656 2556
Size: 1656 900
Bsu36I CC'TnA_GG
Cuts at: 0 2087 2556
Size: 2087 469
BtgI C'CryG_G
Cuts at: 0 247 260 371 2556
Size: 247 13 111 2185
Fragments arranged by size:
2185 247 111 13
BtsI GCAGTG_nn'
Cuts at: 0 282 2072 2556
Size: 282 1790 484
Fragments arranged by size:
1790 484 282
DraIII CAC_nnn'GTG
Cuts at: 0 1890 2556
Size: 1890 666
EaeI y'GGCC_r
Cuts at: 0 356 627 1290 1584 1646 1961 2117 2472
Size: 356 271 663 294 62 315 156 355
Cuts at: 2472 2556
Size: 84
Fragments arranged by size:
663 356 355 315 294 271 156 84
62
EagI C'GGCC_G
Cuts at: 0 356 627 2556
Size: 356 271 1929
Fragments arranged by size:
1929 356 271
EarI CTCTTCn'nnn_
Cuts at: 0 1169 2549 2556
Size: 1169 1380 7
Fragments arranged by size:
1380 1169 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 379 546 2419 2556
Size: 379 167 1873 137
Fragments arranged by size:
1873 379 167 137
EcoICRI GAG'CTC
Cuts at: 0 486 2556
Size: 486 2070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 726 876 905 1529 1799 1969 2077
Size: 418 308 150 29 624 270 170 108
Cuts at: 2077 2116 2305 2422 2507 2556
Size: 39 189 117 85 49
Fragments arranged by size:
624 418 308 270 189 170 150 117
108 85 49 39 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 730 1174 2437 2556
Size: 730 444 1263 119
Fragments arranged by size:
1263 730 444 119
EcoO109I rG'GnC_Cy
Cuts at: 0 6 13 211 297 305 634 869 1179
Size: 6 7 198 86 8 329 235 310
Cuts at: 1179 1228 1229 1549 1811 1989 2513 2556
Size: 49 1 320 262 178 524 43
Fragments arranged by size:
524 329 320 310 262 235 198 178
86 49 43 8 7 6 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 681 2556
Size: 681 1875
HaeII r_GCGC'y
Cuts at: 0 793 920 2046 2556
Size: 793 127 1126 510
Fragments arranged by size:
1126 793 510 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 853 885 1452 1484 1795 1827
Size: 16 32 805 32 567 32 311 32
Cuts at: 1827 2234 2266 2505 2537 2556
Size: 407 32 239 32 19
Fragments arranged by size:
805 567 407 311 239 32 32 32
32 32 32 19 16
KasI G'GCGC_C
Cuts at: 0 2042 2556
Size: 2042 514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 886 892 2556
Size: 798 88 6 1664
Fragments arranged by size:
1664 798 88 6
MscI TGG'CCA
Cuts at: 0 1292 1586 1648 2474 2556
Size: 1292 294 62 826 82
Fragments arranged by size:
1292 826 294 82 62
MslI CAynn'nnrTG
Cuts at: 0 2031 2556
Size: 2031 525
MspA1I CmG'CkG
Cuts at: 0 249 262 339 373 429 447 654 1480
Size: 249 13 77 34 56 18 207 826
Cuts at: 1480 1513 2259 2556
Size: 33 746 297
Fragments arranged by size:
826 746 297 249 207 77 56 34
33 18 13
NaeI GCC'GGC
Cuts at: 0 404 593 627 713 1844 2556
Size: 404 189 34 86 1131 712
Fragments arranged by size:
1131 712 404 189 86 34
NarI GG'CG_CC
Cuts at: 0 2043 2556
Size: 2043 513
NgoMIV G'CCGG_C
Cuts at: 0 402 591 625 711 1842 2556
Size: 402 189 34 86 1131 714
Fragments arranged by size:
1131 714 402 189 86 34
PasI CC'CwG_GG
Cuts at: 0 9 757 1224 1707 2509 2556
Size: 9 748 467 483 802 47
Fragments arranged by size:
802 748 483 467 47 9
PfoI T'CCnGG_A
Cuts at: 0 458 1332 2556
Size: 458 874 1224
Fragments arranged by size:
1224 874 458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 847 879 2556
Size: 454 32 361 32 1677
Fragments arranged by size:
1677 454 361 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 13 297 305 634 869 1549 1811 1989
Size: 13 284 8 329 235 680 262 178
Cuts at: 1989 2513 2556
Size: 524 43
Fragments arranged by size:
680 524 329 284 262 235 178 43
13 8
PspOMI G'GGCC_C
Cuts at: 0 210 760 1228 2556
Size: 210 550 468 1328
Fragments arranged by size:
1328 550 468 210
PspXI vC'TCGA_Gb
Cuts at: 0 408 1624 2556
Size: 408 1216 932
Fragments arranged by size:
1216 932 408
PstI C_TGCA'G
Cuts at: 0 1074 1344 1485 2018 2067 2240 2258 2556
Size: 1074 270 141 533 49 173 18 298
Fragments arranged by size:
1074 533 298 270 173 141 49 18
PvuII CAG'CTG
Cuts at: 0 1480 1513 2556
Size: 1480 33 1043
Fragments arranged by size:
1480 1043 33
SacI G_AGCT'C
Cuts at: 0 488 2556
Size: 488 2068
SacII CC_GC'GG
Cuts at: 0 250 263 374 2556
Size: 250 13 111 2182
Fragments arranged by size:
2182 250 111 13
SanDI GG'GwC_CC
Cuts at: 0 305 634 2556
Size: 305 329 1922
Fragments arranged by size:
1922 329 305
ScaI AGT'ACT
Cuts at: 0 1797 2556
Size: 1797 759
SfcI C'TryA_G
Cuts at: 0 96 1070 1340 1379 1481 2014 2063 2236
Size: 96 974 270 39 102 533 49 173
Cuts at: 2236 2254 2556
Size: 18 302
Fragments arranged by size:
974 533 302 270 173 102 96 49
39 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 412 1608 2197 2556
Size: 412 1196 589 359
Fragments arranged by size:
1196 589 412 359
SfoI GGC'GCC
Cuts at: 0 2044 2556
Size: 2044 512
SmaI CCC'GGG
Cuts at: 0 209 302 311 575 604 2194 2556
Size: 209 93 9 264 29 1590 362
Fragments arranged by size:
1590 362 264 209 93 29 9
SmlI C'TyrA_G
Cuts at: 0 408 1624 2556
Size: 408 1216 932
Fragments arranged by size:
1216 932 408
SrfI GCCC'GGGC
Cuts at: 0 209 2194 2556
Size: 209 1985 362
Fragments arranged by size:
1985 362 209
StuI AGG'CCT
Cuts at: 0 736 2077 2227 2556
Size: 736 1341 150 329
Fragments arranged by size:
1341 736 329 150
StyI C'CwwG_G
Cuts at: 0 770 843 987 1520 2276 2469 2556
Size: 770 73 144 533 756 193 87
Fragments arranged by size:
770 756 533 193 144 87 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 718 1846 1897 2556
Size: 718 1128 51 659
Fragments arranged by size:
1128 718 659 51
TatI w'GTAC_w
Cuts at: 0 1795 2556
Size: 1795 761
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 2556
Size: 538 32 1986
Fragments arranged by size:
1986 538 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 1411 2556
Size: 54 1357 1145
Fragments arranged by size:
1357 1145 54
XhoI C'TCGA_G
Cuts at: 0 408 1624 2556
Size: 408 1216 932
Fragments arranged by size:
1216 932 408
XmaI C'CCGG_G
Cuts at: 0 207 300 309 573 602 2192 2556
Size: 207 93 9 264 29 1590 364
Fragments arranged by size:
1590 364 264 207 93 29 9
ZraI GAC'GTC
Cuts at: 0 467 2556
Size: 467 2089
Enzymes that do cut:
AatII AcuI AfeI AgeI AhdI AleI AlfI AloI
AlwNI ApaI AvaI BanI BanII BbeI BciVI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV PasI PfoI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SanDI ScaI SfcI SfiI
SfoI SmaI SmlI SrfI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI
BsiWI BspEI BspHI BsrGI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PshAI PsiI PsrI PvuI
RsrII SalI SapI SbfI SexAI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I XbaI XmnI