Restriction map for
pF1KSDA1957
(Linear) (Six-base) MAPSORT of: KIAA1957.seq Check: 6778 from: 1 to: 1263
KIAA1957.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 983 1263
Size: 983 280
AatII G_ACGT'C
Cuts at: 0 439 1263
Size: 439 824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 615 1263
Size: 331 284 648
Fragments arranged by size:
648 331 284
AflIII A'CryG_T
Cuts at: 0 14 1263
Size: 14 1249
AhdI GACnn_n'nnGTC
Cuts at: 0 360 654 1263
Size: 360 294 609
Fragments arranged by size:
609 360 294
AleI CACnn'nnGTG
Cuts at: 0 1084 1263
Size: 1084 179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 1263
Size: 322 941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 1263
Size: 478 32 753
Fragments arranged by size:
753 478 32
AlwNI CAG_nnn'CTG
Cuts at: 0 231 1263
Size: 231 1032
ApaI G_GGCC'C
Cuts at: 0 107 178 211 589 609 913 1145 1263
Size: 107 71 33 378 20 304 232 118
Fragments arranged by size:
378 304 232 118 107 71 33 20
ApaLI G'TGCA_C
Cuts at: 0 970 1263
Size: 970 293
AvaI C'yCGr_G
Cuts at: 0 62 171 820 868 914 1219 1263
Size: 62 109 649 48 46 305 44
Fragments arranged by size:
649 305 109 62 48 46 44
BanI G'GyrC_C
Cuts at: 0 400 420 545 830 849 1050 1263
Size: 400 20 125 285 19 201 213
Fragments arranged by size:
400 285 213 201 125 20 19
BanII G_rGCy'C
Cuts at: 0 107 178 211 268 469 516 532 589
Size: 107 71 33 57 201 47 16 57
Cuts at: 589 609 619 913 1145 1263
Size: 20 10 294 232 118
Fragments arranged by size:
294 232 201 118 107 71 57 57
47 33 20 16 10
BbeI G_GCGC'C
Cuts at: 0 549 834 1263
Size: 549 285 429
Fragments arranged by size:
549 429 285
BbsI GAAGACnn'nnnn_
Cuts at: 0 1101 1263
Size: 1101 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1065 1099 1263
Size: 1065 34 164
Fragments arranged by size:
1065 164 34
BglI GCCn_nnn'nGGC
Cuts at: 0 241 286 457 779 917 955 1263
Size: 241 45 171 322 138 38 308
Fragments arranged by size:
322 308 241 171 138 45 38
Bme1580I G_kGCm'C
Cuts at: 0 107 178 211 425 589 609 852 913
Size: 107 71 33 214 164 20 243 61
Cuts at: 913 974 1145 1263
Size: 61 171 118
Fragments arranged by size:
243 214 171 164 118 107 71 61
61 33 20
BmgBI CAC'GTC
Cuts at: 0 218 1263
Size: 218 1045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1263
Size: 1186 77
BsaAI yAC'GTr
Cuts at: 0 330 906 1263
Size: 330 576 357
Fragments arranged by size:
576 357 330
BsaBI GATnn'nnATC
Cuts at: 0 337 1263
Size: 337 926
BsaHI Gr'CG_yC
Cuts at: 0 127 380 436 546 812 831 1013 1263
Size: 127 253 56 110 266 19 182 250
Fragments arranged by size:
266 253 250 182 127 110 56 19
BsaWI w'CCGG_w
Cuts at: 0 1154 1263
Size: 1154 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 478 508 1263
Size: 79 30 369 30 755
Fragments arranged by size:
755 369 79 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 1263
Size: 382 881
BseYI C'CCAG_C
Cuts at: 0 204 1263
Size: 204 1059
BsiEI CG_ry'CG
Cuts at: 0 624 665 1002 1263
Size: 624 41 337 261
Fragments arranged by size:
624 337 261 41
BsiHKAI G_wGCw'C
Cuts at: 0 509 896 974 1263
Size: 509 387 78 289
Fragments arranged by size:
509 387 289 78
Bsp1286I G_dGCh'C
Cuts at: 0 107 178 211 268 425 469 509 516
Size: 107 71 33 57 157 44 40 7
Cuts at: 516 532 589 609 619 852 896 913 974
Size: 16 57 20 10 233 44 17 61
Cuts at: 974 1145 1263
Size: 171 118
Fragments arranged by size:
233 171 157 118 107 71 61 57
57 44 44 40 33 20 17 16
10 7
BspEI T'CCGG_A
Cuts at: 0 1154 1263
Size: 1154 109
BspHI T'CATG_A
Cuts at: 0 611 1263
Size: 611 652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 983 1263
Size: 983 280
BsrBI CCG'CTC
Cuts at: 0 30 701 1263
Size: 30 671 562
Fragments arranged by size:
671 562 30
BsrDI GCAATG_nn'
Cuts at: 0 123 1263
Size: 123 1140
BsrFI r'CCGG_y
Cuts at: 0 827 949 1263
Size: 827 122 314
Fragments arranged by size:
827 314 122
BsrGI T'GTAC_A
Cuts at: 0 110 1263
Size: 110 1153
BssSI C'ACGA_G
Cuts at: 0 474 1263
Size: 474 789
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1028 1263
Size: 1028 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 886 1263
Size: 886 377
BstYI r'GATC_y
Cuts at: 0 137 1263
Size: 137 1126
Bsu36I CC'TnA_GG
Cuts at: 0 1168 1263
Size: 1168 95
BtgI C'CryG_G
Cuts at: 0 179 259 767 777 1263
Size: 179 80 508 10 486
Fragments arranged by size:
508 486 179 80 10
DraIII CAC_nnn'GTG
Cuts at: 0 768 1263
Size: 768 495
EaeI y'GGCC_r
Cuts at: 0 458 621 662 935 951 980 1263
Size: 458 163 41 273 16 29 283
Fragments arranged by size:
458 283 273 163 41 29 16
EagI C'GGCC_G
Cuts at: 0 621 662 1263
Size: 621 41 601
Fragments arranged by size:
621 601 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 615 1263
Size: 331 284 648
Fragments arranged by size:
648 331 284
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 1263
Size: 310 953
EcoO109I rG'GnC_Cy
Cuts at: 0 103 104 175 208 585 586 605 606
Size: 103 1 71 33 377 1 19 1
Cuts at: 606 656 683 910 990 1141 1263
Size: 50 27 227 80 151 122
Fragments arranged by size:
377 227 151 122 103 80 71 50
33 27 19 1 1 1
FseI GG_CCGG'CC
Cuts at: 0 953 1263
Size: 953 310
HaeII r_GCGC'y
Cuts at: 0 549 834 1263
Size: 549 285 429
Fragments arranged by size:
549 429 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 346 378 640 672 1263
Size: 79 32 235 32 262 32 591
Fragments arranged by size:
591 262 235 79 32 32 32
HincII GTy'rAC
Cuts at: 0 125 1263
Size: 125 1138
KasI G'GCGC_C
Cuts at: 0 545 830 1263
Size: 545 285 433
Fragments arranged by size:
545 433 285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 1263
Size: 200 1063
MslI CAynn'nnrTG
Cuts at: 0 1084 1263
Size: 1084 179
MspA1I CmG'CkG
Cuts at: 0 181 559 1263
Size: 181 378 704
Fragments arranged by size:
704 378 181
NaeI GCC'GGC
Cuts at: 0 829 951 1263
Size: 829 122 312
Fragments arranged by size:
829 312 122
NarI GG'CG_CC
Cuts at: 0 546 831 1263
Size: 546 285 432
Fragments arranged by size:
546 432 285
NgoMIV G'CCGG_C
Cuts at: 0 827 949 1263
Size: 827 122 314
Fragments arranged by size:
827 314 122
NspI r_CATG'y
Cuts at: 0 18 1263
Size: 18 1245
PasI CC'CwG_GG
Cuts at: 0 396 535 590 1263
Size: 396 139 55 673
Fragments arranged by size:
673 396 139 55
PciI A'CATG_T
Cuts at: 0 14 1263
Size: 14 1249
PflMI CCAn_nnn'nTGG
Cuts at: 0 17 1263
Size: 17 1246
PmlI CAC'GTG
Cuts at: 0 330 906 1263
Size: 330 576 357
Fragments arranged by size:
576 357 330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 478 510 1263
Size: 220 32 226 32 753
Fragments arranged by size:
753 226 220 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 656 683 1263
Size: 656 27 580
Fragments arranged by size:
656 580 27
PspOMI G'GGCC_C
Cuts at: 0 103 174 207 585 605 909 1141 1263
Size: 103 71 33 378 20 304 232 122
Fragments arranged by size:
378 304 232 122 103 71 33 20
PstI C_TGCA'G
Cuts at: 0 1067 1263
Size: 1067 196
PvuII CAG'CTG
Cuts at: 0 559 1263
Size: 559 704
SacII CC_GC'GG
Cuts at: 0 182 1263
Size: 182 1081
SanDI GG'GwC_CC
Cuts at: 0 683 1263
Size: 683 580
SfcI C'TryA_G
Cuts at: 0 1063 1263
Size: 1063 200
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 241 955 1263
Size: 241 714 308
Fragments arranged by size:
714 308 241
SfoI GGC'GCC
Cuts at: 0 547 832 1263
Size: 547 285 431
Fragments arranged by size:
547 431 285
SmaI CCC'GGG
Cuts at: 0 64 822 1263
Size: 64 758 441
Fragments arranged by size:
758 441 64
SmlI C'TyrA_G
Cuts at: 0 1201 1263
Size: 1201 62
StuI AGG'CCT
Cuts at: 0 94 1263
Size: 94 1169
StyI C'CwwG_G
Cuts at: 0 98 425 563 743 1263
Size: 98 327 138 180 520
Fragments arranged by size:
520 327 180 138 98
TatI w'GTAC_w
Cuts at: 0 110 1263
Size: 110 1153
XmaI C'CCGG_G
Cuts at: 0 62 820 1263
Size: 62 758 443
Fragments arranged by size:
758 443 62
ZraI GAC'GTC
Cuts at: 0 437 1263
Size: 437 826
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BcgI BglI Bme1580I BmgBI BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI DraIII EaeI EagI Eco57MI
EcoNI EcoO109I FseI HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacII
SanDI SfcI SfiI SfoI SmaI SmlI StuI StyI
TatI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI BpmI Bpu10I
BsaI BsgI BsiWI BsmI BsmBI BssHII BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EarI
EciI EcoICRI EcoRI EcoRV FalI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SapI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI