Restriction map for
pF1KSDA1932
(Linear) (Six-base) MAPSORT of: KIAA1932.seq Check: 7624 from: 1 to: 2337
KIAA1932.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1555 2337
Size: 1555 782
Acc65I G'GTAC_C
Cuts at: 0 266 2337
Size: 266 2071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1978 2337
Size: 1429 549 359
Fragments arranged by size:
1429 549 359
AfeI AGC'GCT
Cuts at: 0 784 2337
Size: 784 1553
AflIII A'CryG_T
Cuts at: 0 215 1676 2337
Size: 215 1461 661
Fragments arranged by size:
1461 661 215
AhdI GACnn_n'nnGTC
Cuts at: 0 584 2337
Size: 584 1753
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1986 2337
Size: 1986 351
AlwNI CAG_nnn'CTG
Cuts at: 0 135 160 369 1530 1716 2337
Size: 135 25 209 1161 186 621
Fragments arranged by size:
1161 621 209 186 135 25
ApaI G_GGCC'C
Cuts at: 0 482 1293 2010 2337
Size: 482 811 717 327
Fragments arranged by size:
811 717 482 327
ApaLI G'TGCA_C
Cuts at: 0 206 2337
Size: 206 2131
AvaI C'yCGr_G
Cuts at: 0 722 920 1042 1067 1465 1914 2337
Size: 722 198 122 25 398 449 423
Fragments arranged by size:
722 449 423 398 198 122 25
BanI G'GyrC_C
Cuts at: 0 266 1018 1054 1344 1543 2136 2337
Size: 266 752 36 290 199 593 201
Fragments arranged by size:
752 593 290 266 201 199 36
BanII G_rGCy'C
Cuts at: 0 417 482 905 943 1068 1293 1472 2010
Size: 417 65 423 38 125 225 179 538
Cuts at: 2010 2337
Size: 327
Fragments arranged by size:
538 423 417 327 225 179 125 65
38
BbeI G_GCGC'C
Cuts at: 0 1348 2337
Size: 1348 989
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 1098 2337
Size: 32 1066 1239
Fragments arranged by size:
1239 1066 32
BbvCI CC'TCA_GC
Cuts at: 0 234 362 389 437 2337
Size: 234 128 27 48 1900
Fragments arranged by size:
1900 234 128 48 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1750 1784 1858 1892 2337
Size: 1750 34 74 34 445
Fragments arranged by size:
1750 445 74 34 34
BclI T'GATC_A
Cuts at: 0 18 2120 2337
Size: 18 2102 217
Fragments arranged by size:
2102 217 18
BglI GCCn_nnn'nGGC
Cuts at: 0 130 2113 2337
Size: 130 1983 224
Fragments arranged by size:
1983 224 130
BlpI GC'TnA_GC
Cuts at: 0 380 2337
Size: 380 1957
Bme1580I G_kGCm'C
Cuts at: 0 210 482 1023 1293 1546 1590 2010 2337
Size: 210 272 541 270 253 44 420 327
Fragments arranged by size:
541 420 327 272 270 253 210 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 2224 2337
Size: 313 1911 113
Fragments arranged by size:
1911 313 113
BmtI G_CTAG'C
Cuts at: 0 971 2337
Size: 971 1366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 602 912 1146 1402 1516 2337
Size: 437 165 310 234 256 114 821
Fragments arranged by size:
821 437 310 256 234 165 114
Bpu10I CC'TnA_GC
Cuts at: 0 234 362 389 437 2337
Size: 234 128 27 48 1900
Fragments arranged by size:
1900 234 128 48 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 823 1813 2095 2169 2337
Size: 574 249 990 282 74 168
Fragments arranged by size:
990 574 282 249 168 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 1756 2337
Size: 592 1164 581
Fragments arranged by size:
1164 592 581
BsaAI yAC'GTr
Cuts at: 0 216 2337
Size: 216 2121
BsaHI Gr'CG_yC
Cuts at: 0 91 1039 1345 2337
Size: 91 948 306 992
Fragments arranged by size:
992 948 306 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1132 1162 1192 1222 1432 1462 2337
Size: 1132 30 30 30 210 30 875
Fragments arranged by size:
1132 875 210 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 823 1146 1149 2206 2337
Size: 823 323 3 1057 131
Fragments arranged by size:
1057 823 323 131 3
BseYI C'CCAG_C
Cuts at: 0 61 677 1028 1048 2337
Size: 61 616 351 20 1289
Fragments arranged by size:
1289 616 351 61 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1744 2337
Size: 1744 593
BsiEI CG_ry'CG
Cuts at: 0 1062 1284 2337
Size: 1062 222 1053
Fragments arranged by size:
1062 1053 222
BsiHKAI G_wGCw'C
Cuts at: 0 210 2337
Size: 210 2127
BsmI GAATG_Cn'
Cuts at: 0 2278 2337
Size: 2278 59
BsmBI CGTCTCn'nnnn_
Cuts at: 0 82 2187 2337
Size: 82 2105 150
Fragments arranged by size:
2105 150 82
Bsp1286I G_dGCh'C
Cuts at: 0 210 417 482 905 943 1023 1068 1293
Size: 210 207 65 423 38 80 45 225
Cuts at: 1293 1472 1546 1590 2010 2337
Size: 179 74 44 420 327
Fragments arranged by size:
423 420 327 225 210 207 179 80
74 65 45 44 38
BspHI T'CATG_A
Cuts at: 0 1109 2337
Size: 1109 1228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 531 1555 1580 2337
Size: 531 1024 25 757
Fragments arranged by size:
1024 757 531 25
BsrBI CCG'CTC
Cuts at: 0 1148 1154 2337
Size: 1148 6 1183
Fragments arranged by size:
1183 1148 6
BsrDI GCAATG_nn'
Cuts at: 0 195 1866 2337
Size: 195 1671 471
Fragments arranged by size:
1671 471 195
BsrFI r'CCGG_y
Cuts at: 0 163 1057 2337
Size: 163 894 1280
Fragments arranged by size:
1280 894 163
BssSI C'ACGA_G
Cuts at: 0 100 1949 2071 2337
Size: 100 1849 122 266
Fragments arranged by size:
1849 266 122 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1530 1572 2337
Size: 1530 42 765
Fragments arranged by size:
1530 765 42
BstEII G'GTnAC_C
Cuts at: 0 1792 2337
Size: 1792 545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 499 637 664 1935 2337
Size: 499 138 27 1271 402
Fragments arranged by size:
1271 499 402 138 27
BstYI r'GATC_y
Cuts at: 0 1713 1965 2337
Size: 1713 252 372
Fragments arranged by size:
1713 372 252
Bsu36I CC'TnA_GG
Cuts at: 0 1268 2337
Size: 1268 1069
BtgI C'CryG_G
Cuts at: 0 485 1928 2337
Size: 485 1443 409
Fragments arranged by size:
1443 485 409
BtsI GCAGTG_nn'
Cuts at: 0 1509 2337
Size: 1509 828
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 532 567 2337
Size: 532 35 1770
Fragments arranged by size:
1770 532 35
DraIII CAC_nnn'GTG
Cuts at: 0 1245 2337
Size: 1245 1092
EaeI y'GGCC_r
Cuts at: 0 5 137 200 2337
Size: 5 132 63 2137
Fragments arranged by size:
2137 132 63 5
EarI CTCTTCn'nnn_
Cuts at: 0 1131 1324 1796 2167 2331 2337
Size: 1131 193 472 371 164 6
Fragments arranged by size:
1131 472 371 193 164 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2164 2337
Size: 2164 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 602 912 1146 1402 1429 1516 1978
Size: 437 165 310 234 256 27 87 462
Cuts at: 1978 2337
Size: 359
Fragments arranged by size:
462 437 359 310 256 234 165 87
27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 986 1161 2337
Size: 986 175 1176
Fragments arranged by size:
1176 986 175
EcoO109I rG'GnC_Cy
Cuts at: 0 132 478 713 1166 1289 1392 2007 2337
Size: 132 346 235 453 123 103 615 330
Fragments arranged by size:
615 453 346 330 235 132 123 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 630 2337
Size: 630 1707
HaeII r_GCGC'y
Cuts at: 0 786 1348 1381 2337
Size: 786 562 33 956
Fragments arranged by size:
956 786 562 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 570 602 1108 1132 1140 1164
Size: 328 32 210 32 506 24 8 24
Cuts at: 1164 1186 1218 1323 1355 2337
Size: 22 32 105 32 982
Fragments arranged by size:
982 506 328 210 105 32 32 32
32 24 24 22 8
KasI G'GCGC_C
Cuts at: 0 1344 2337
Size: 1344 993
KpnI G_GTAC'C
Cuts at: 0 270 2337
Size: 270 2067
MluI A'CGCG_T
Cuts at: 0 1676 2337
Size: 1676 661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 785 1235 2337
Size: 668 117 450 1102
Fragments arranged by size:
1102 668 450 117
MscI TGG'CCA
Cuts at: 0 139 202 2337
Size: 139 63 2135
Fragments arranged by size:
2135 139 63
MspA1I CmG'CkG
Cuts at: 0 369 549 710 2337
Size: 369 180 161 1627
Fragments arranged by size:
1627 369 180 161
NaeI GCC'GGC
Cuts at: 0 165 2337
Size: 165 2172
NarI GG'CG_CC
Cuts at: 0 1345 2337
Size: 1345 992
NdeI CA'TA_TG
Cuts at: 0 1295 2337
Size: 1295 1042
NgoMIV G'CCGG_C
Cuts at: 0 163 2337
Size: 163 2174
NheI G'CTAG_C
Cuts at: 0 967 2337
Size: 967 1370
NspI r_CATG'y
Cuts at: 0 345 2061 2337
Size: 345 1716 276
Fragments arranged by size:
1716 345 276
PasI CC'CwG_GG
Cuts at: 0 574 2010 2337
Size: 574 1436 327
Fragments arranged by size:
1436 574 327
PflMI CCAn_nnn'nTGG
Cuts at: 0 890 1986 2337
Size: 890 1096 351
Fragments arranged by size:
1096 890 351
PpuMI rG'GwC_Cy
Cuts at: 0 713 1166 1392 2337
Size: 713 453 226 945
Fragments arranged by size:
945 713 453 226
PspOMI G'GGCC_C
Cuts at: 0 478 1289 2006 2337
Size: 478 811 717 331
Fragments arranged by size:
811 717 478 331
PspXI vC'TCGA_Gb
Cuts at: 0 1465 2337
Size: 1465 872
PstI C_TGCA'G
Cuts at: 0 854 866 1484 1535 2337
Size: 854 12 618 51 802
Fragments arranged by size:
854 802 618 51 12
PvuII CAG'CTG
Cuts at: 0 369 549 2337
Size: 369 180 1788
Fragments arranged by size:
1788 369 180
SapI GCTCTTCn'nnn_
Cuts at: 0 1131 2167 2337
Size: 1131 1036 170
Fragments arranged by size:
1131 1036 170
ScaI AGT'ACT
Cuts at: 0 256 2337
Size: 256 2081
SfcI C'TryA_G
Cuts at: 0 294 850 862 1480 1531 2337
Size: 294 556 12 618 51 806
Fragments arranged by size:
806 618 556 294 51 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 130 2337
Size: 130 2207
SfoI GGC'GCC
Cuts at: 0 1346 2337
Size: 1346 991
SmaI CCC'GGG
Cuts at: 0 724 922 1044 1069 2337
Size: 724 198 122 25 1268
Fragments arranged by size:
1268 724 198 122 25
SmlI C'TyrA_G
Cuts at: 0 589 838 1465 1828 2110 2184 2337
Size: 589 249 627 363 282 74 153
Fragments arranged by size:
627 589 363 282 249 153 74
StuI AGG'CCT
Cuts at: 0 496 2337
Size: 496 1841
StyI C'CwwG_G
Cuts at: 0 1781 1985 2287 2337
Size: 1781 204 302 50
Fragments arranged by size:
1781 302 204 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4 643 2337
Size: 4 639 1694
Fragments arranged by size:
1694 639 4
TatI w'GTAC_w
Cuts at: 0 47 254 275 2337
Size: 47 207 21 2062
Fragments arranged by size:
2062 207 47 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 697 729 1192 1224 1432 1464 2337
Size: 697 32 463 32 208 32 873
Fragments arranged by size:
873 697 463 208 32 32 32
XhoI C'TCGA_G
Cuts at: 0 1465 2337
Size: 1465 872
XmaI C'CCGG_G
Cuts at: 0 722 920 1042 1067 2337
Size: 722 198 122 25 1270
Fragments arranged by size:
1270 722 198 122 25
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI Bme1580I BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI
MluI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV
NheI NspI PasI PflMI PpuMI PspOMI PspXI PstI
PvuII SapI ScaI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AleI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI
BglII BmgBI BplI BsaBI BsaWI BsiWI BspEI BsrGI
BssHII BstBI BstZ17I BtgZI ClaI DraI DrdI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MslI NcoI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XcmI XmnI ZraI