Restriction map for
pF1KA1926
(Linear) (Six-base) MAPSORT of: KIAA1926.seq Check: 9262 from: 1 to: 1206
KIAA1926.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 153 1206
Size: 153 1053
AatII G_ACGT'C
Cuts at: 0 293 1206
Size: 293 913
AccI GT'mk_AC
Cuts at: 0 1082 1206
Size: 1082 124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1206
Size: 853 353
AleI CACnn'nnGTG
Cuts at: 0 179 506 1206
Size: 179 327 700
Fragments arranged by size:
700 327 179
AlwNI CAG_nnn'CTG
Cuts at: 0 76 157 1206
Size: 76 81 1049
Fragments arranged by size:
1049 81 76
ApaLI G'TGCA_C
Cuts at: 0 109 1206
Size: 109 1097
AvaI C'yCGr_G
Cuts at: 0 11 677 1206
Size: 11 666 529
Fragments arranged by size:
666 529 11
BanI G'GyrC_C
Cuts at: 0 184 1116 1206
Size: 184 932 90
Fragments arranged by size:
932 184 90
BanII G_rGCy'C
Cuts at: 0 136 686 1206
Size: 136 550 520
Fragments arranged by size:
550 520 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 285 1206
Size: 285 921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 995 1029 1165 1199 1206
Size: 961 34 34 136 34 7
Fragments arranged by size:
961 136 34 34 34 7
BlpI GC'TnA_GC
Cuts at: 0 347 1206
Size: 347 859
Bme1580I G_kGCm'C
Cuts at: 0 113 1206
Size: 113 1093
BmgBI CAC'GTC
Cuts at: 0 803 1203 1206
Size: 803 400 3
Fragments arranged by size:
803 400 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 1206
Size: 150 1056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1082 1206
Size: 139 943 124
Fragments arranged by size:
943 139 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1206
Size: 235 971
BsaAI yAC'GTr
Cuts at: 0 114 1206
Size: 114 1092
BsaHI Gr'CG_yC
Cuts at: 0 56 242 290 788 1058 1079 1206
Size: 56 186 48 498 270 21 127
Fragments arranged by size:
498 270 186 127 56 48 21
BsaWI w'CCGG_w
Cuts at: 0 859 1107 1206
Size: 859 248 99
Fragments arranged by size:
859 248 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 1206
Size: 112 30 1064
Fragments arranged by size:
1064 112 30
BseYI C'CCAG_C
Cuts at: 0 311 1127 1133 1139 1157 1206
Size: 311 816 6 6 18 49
Fragments arranged by size:
816 311 49 18 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 734 936 1183 1206
Size: 577 157 202 247 23
Fragments arranged by size:
577 247 202 157 23
BsiEI CG_ry'CG
Cuts at: 0 896 1206
Size: 896 310
BsiHKAI G_wGCw'C
Cuts at: 0 113 1107 1206
Size: 113 994 99
Fragments arranged by size:
994 113 99
BsmI GAATG_Cn'
Cuts at: 0 322 1206
Size: 322 884
Bsp1286I G_dGCh'C
Cuts at: 0 113 136 686 1107 1206
Size: 113 23 550 421 99
Fragments arranged by size:
550 421 113 99 23
BspEI T'CCGG_A
Cuts at: 0 859 1206
Size: 859 347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 153 818 1206
Size: 153 665 388
Fragments arranged by size:
665 388 153
BsrFI r'CCGG_y
Cuts at: 0 671 1206
Size: 671 535
BssHII G'CGCG_C
Cuts at: 0 32 546 868 870 982 1009 1169 1171
Size: 32 514 322 2 112 27 160 2
Cuts at: 1171 1206
Size: 35
Fragments arranged by size:
514 322 160 112 35 32 27 2
2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 444 508 1206
Size: 181 263 64 698
Fragments arranged by size:
698 263 181 64
BtgI C'CryG_G
Cuts at: 0 44 177 228 336 507 896 956 1206
Size: 44 133 51 108 171 389 60 250
Fragments arranged by size:
389 250 171 133 108 60 51 44
BtsI GCAGTG_nn'
Cuts at: 0 984 1206
Size: 984 222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 248 283 1206
Size: 248 35 923
Fragments arranged by size:
923 248 35
EaeI y'GGCC_r
Cuts at: 0 93 893 899 968 1206
Size: 93 800 6 69 238
Fragments arranged by size:
800 238 93 69 6
EagI C'GGCC_G
Cuts at: 0 893 1206
Size: 893 313
EarI CTCTTCn'nnn_
Cuts at: 0 1078 1206
Size: 1078 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 853 1082 1206
Size: 139 714 229 124
Fragments arranged by size:
714 229 139 124
EcoO109I rG'GnC_Cy
Cuts at: 0 307 741 1206
Size: 307 434 465
Fragments arranged by size:
465 434 307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 546 1206
Size: 546 660
FspI TGC'GCA
Cuts at: 0 748 1039 1206
Size: 748 291 167
Fragments arranged by size:
748 291 167
HaeII r_GCGC'y
Cuts at: 0 157 420 1206
Size: 157 263 786
Fragments arranged by size:
786 263 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 1206
Size: 112 32 1062
Fragments arranged by size:
1062 112 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1206
Size: 830 376
MscI TGG'CCA
Cuts at: 0 901 1206
Size: 901 305
MslI CAynn'nnrTG
Cuts at: 0 179 506 1206
Size: 179 327 700
Fragments arranged by size:
700 327 179
MspA1I CmG'CkG
Cuts at: 0 46 276 591 840 915 939 950 958
Size: 46 230 315 249 75 24 11 8
Cuts at: 958 1206
Size: 248
Fragments arranged by size:
315 249 248 230 75 46 24 11
8
PfoI T'CCnGG_A
Cuts at: 0 1052 1098 1206
Size: 1052 46 108
Fragments arranged by size:
1052 108 46
PmlI CAC'GTG
Cuts at: 0 114 1206
Size: 114 1092
PstI C_TGCA'G
Cuts at: 0 356 585 938 1206
Size: 356 229 353 268
Fragments arranged by size:
356 353 268 229
PvuII CAG'CTG
Cuts at: 0 591 915 939 1206
Size: 591 324 24 267
Fragments arranged by size:
591 324 267 24
SacII CC_GC'GG
Cuts at: 0 47 959 1206
Size: 47 912 247
Fragments arranged by size:
912 247 47
SapI GCTCTTCn'nnn_
Cuts at: 0 1078 1206
Size: 1078 128
SfcI C'TryA_G
Cuts at: 0 352 581 934 1206
Size: 352 229 353 272
Fragments arranged by size:
353 352 272 229
SmaI CCC'GGG
Cuts at: 0 679 1206
Size: 679 527
SmlI C'TyrA_G
Cuts at: 0 250 1206
Size: 250 956
StuI AGG'CCT
Cuts at: 0 1029 1206
Size: 1029 177
StyI C'CwwG_G
Cuts at: 0 902 1206
Size: 902 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 13 1206
Size: 13 1193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 696 728 1206
Size: 696 32 478
Fragments arranged by size:
696 478 32
Tth111I GACn'n_nGTC
Cuts at: 0 241 1206
Size: 241 965
XmaI C'CCGG_G
Cuts at: 0 677 1206
Size: 677 529
ZraI GAC'GTC
Cuts at: 0 291 1206
Size: 291 915
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlwNI ApaLI AvaI
BanI BanII BbsI BcgI BlpI Bme1580I BmgBI BplI
BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI BssHII
BstXI BtgI BtsI CspCI EaeI EagI EarI Eco57MI
EcoO109I FalI FspI HaeII Hin4I MmeI MscI MslI
MspA1I PfoI PmlI PstI PvuII SacII SapI SfcI
SmaI SmlI StuI StyI TaqII TstI Tth111I XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII
BmrI BmtI Bpu10I BsaI BsaBI BseRI BsiWI BsmBI
BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI
XcmI XhoI XmnI