Restriction map for
pF1KSDA1911
(Linear) (Six-base) MAPSORT of: KIAA1911.seq Check: 9479 from: 1 to: 2520
KIAA1911.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 1128 1876 2520
Size: 1128 748 644
Fragments arranged by size:
1128 748 644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1400 2036 2520
Size: 743 657 636 484
Fragments arranged by size:
743 657 636 484
AflIII A'CryG_T
Cuts at: 0 519 2520
Size: 519 2001
AleI CACnn'nnGTG
Cuts at: 0 2100 2520
Size: 2100 420
AlwNI CAG_nnn'CTG
Cuts at: 0 853 1911 2290 2520
Size: 853 1058 379 230
Fragments arranged by size:
1058 853 379 230
ApoI r'AATT_y
Cuts at: 0 30 66 80 419 594 1192 1530 1636
Size: 30 36 14 339 175 598 338 106
Cuts at: 1636 1970 2520
Size: 334 550
Fragments arranged by size:
598 550 339 338 334 175 106 36
30 14
AseI AT'TA_AT
Cuts at: 0 248 581 1373 1394 1415 2501 2520
Size: 248 333 792 21 21 1086 19
Fragments arranged by size:
1086 792 333 248 21 21 19
BanII G_rGCy'C
Cuts at: 0 1437 2520
Size: 1437 1083
BbsI GAAGACnn'nnnn_
Cuts at: 0 694 2024 2520
Size: 694 1330 496
Fragments arranged by size:
1330 694 496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 699 733 1069 1103 2520
Size: 699 34 336 34 1417
Fragments arranged by size:
1417 699 336 34 34
BfrBI ATG'CAT
Cuts at: 0 1287 2520
Size: 1287 1233
BlpI GC'TnA_GC
Cuts at: 0 1149 2520
Size: 1149 1371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 51 2520
Size: 51 2469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 2520
Size: 895 1625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1211 2520
Size: 1211 1309
BsaI GGTCTCn'nnnn_
Cuts at: 0 1021 2520
Size: 1021 1499
BsaBI GATnn'nnATC
Cuts at: 0 569 2520
Size: 569 1951
BsaWI w'CCGG_w
Cuts at: 0 356 2520
Size: 356 2164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 2520
Size: 1225 1295
BsmI GAATG_Cn'
Cuts at: 0 1874 2371 2520
Size: 1874 497 149
Fragments arranged by size:
1874 497 149
BsmBI CGTCTCn'nnnn_
Cuts at: 0 695 2520
Size: 695 1825
Bsp1286I G_dGCh'C
Cuts at: 0 1437 2520
Size: 1437 1083
BsrDI GCAATG_nn'
Cuts at: 0 1087 2520
Size: 1087 1433
BstYI r'GATC_y
Cuts at: 0 667 2427 2520
Size: 667 1760 93
Fragments arranged by size:
1760 667 93
BstZ17I GTA'TAC
Cuts at: 0 1129 1877 2520
Size: 1129 748 643
Fragments arranged by size:
1129 748 643
Bsu36I CC'TnA_GG
Cuts at: 0 2371 2520
Size: 2371 149
BtsI GCAGTG_nn'
Cuts at: 0 481 1701 2520
Size: 481 1220 819
Fragments arranged by size:
1220 819 481
EaeI y'GGCC_r
Cuts at: 0 1454 2520
Size: 1454 1066
EarI CTCTTCn'nnn_
Cuts at: 0 520 693 1019 2134 2198 2520
Size: 520 173 326 1115 64 322
Fragments arranged by size:
1115 520 326 322 173 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 895 1400 2036 2520
Size: 743 152 505 636 484
Fragments arranged by size:
743 636 505 484 152
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1241 2038 2520
Size: 1241 797 482
Fragments arranged by size:
1241 797 482
EcoRI G'AATT_C
Cuts at: 0 30 66 1970 2520
Size: 30 36 1904 550
Fragments arranged by size:
1904 550 36 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2227 2520
Size: 2227 293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1504 1536 2520
Size: 1504 32 984
Fragments arranged by size:
1504 984 32
HincII GTy'rAC
Cuts at: 0 2046 2067 2520
Size: 2046 21 453
Fragments arranged by size:
2046 453 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1199 2317 2520
Size: 1190 9 1118 203
Fragments arranged by size:
1190 1118 203 9
MscI TGG'CCA
Cuts at: 0 1456 2520
Size: 1456 1064
MslI CAynn'nnrTG
Cuts at: 0 2100 2520
Size: 2100 420
NsiI A_TGCA'T
Cuts at: 0 1289 2520
Size: 1289 1231
NspI r_CATG'y
Cuts at: 0 523 2520
Size: 523 1997
PciI A'CATG_T
Cuts at: 0 519 2520
Size: 519 2001
PflMI CCAn_nnn'nTGG
Cuts at: 0 1545 2520
Size: 1545 975
PsiI TTA'TAA
Cuts at: 0 1933 2520
Size: 1933 587
ScaI AGT'ACT
Cuts at: 0 486 536 2464 2520
Size: 486 50 1928 56
Fragments arranged by size:
1928 486 56 50
SfcI C'TryA_G
Cuts at: 0 2190 2520
Size: 2190 330
SmlI C'TyrA_G
Cuts at: 0 1226 2520
Size: 1226 1294
TatI w'GTAC_w
Cuts at: 0 199 321 484 534 1306 2462 2520
Size: 199 122 163 50 772 1156 58
Fragments arranged by size:
1156 772 199 163 122 58 50
XmnI GAAnn'nnTTC
Cuts at: 0 1722 1970 2520
Size: 1722 248 550
Fragments arranged by size:
1722 550 248
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI AseI BanII
BbsI BcgI BfrBI BlpI BplI BpmI BpuEI BsaI
BsaBI BsaWI BsgI BsmI BsmBI Bsp1286I BsrDI BstYI
BstZ17I Bsu36I BtsI EaeI EarI Eco57MI EcoNI EcoRI
FalI Hin4I HincII MmeI MscI MslI NsiI NspI
PciI PflMI PsiI ScaI SfcI SmlI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BglI
BglII Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI ZraI