Restriction map for
pF1KA1901
(Linear) (Six-base) MAPSORT of: KIAA1901.seq Check: 9472 from: 1 to: 3774
KIAA1901.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 866 1787 1843 2015 2069 2449 2861
Size: 658 208 921 56 172 54 380 412
Cuts at: 2861 3302 3774
Size: 441 472
Fragments arranged by size:
921 658 472 441 412 380 208 172
56 54
AflIII A'CryG_T
Cuts at: 0 351 3148 3774
Size: 351 2797 626
Fragments arranged by size:
2797 626 351
AhdI GACnn_n'nnGTC
Cuts at: 0 742 2618 3774
Size: 742 1876 1156
Fragments arranged by size:
1876 1156 742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3731 3774
Size: 3731 43
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2942 2974 3774
Size: 2942 32 800
Fragments arranged by size:
2942 800 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2428 3774
Size: 2428 1346
ApoI r'AATT_y
Cuts at: 0 368 820 1105 1433 1701 2670 2702 3084
Size: 368 452 285 328 268 969 32 382
Cuts at: 3084 3601 3662 3774
Size: 517 61 112
Fragments arranged by size:
969 517 452 382 368 328 285 268
112 61 32
AvaI C'yCGr_G
Cuts at: 0 1316 1646 2075 2623 2853 3774
Size: 1316 330 429 548 230 921
Fragments arranged by size:
1316 921 548 429 330 230
AvrII C'CTAG_G
Cuts at: 0 727 3774
Size: 727 3047
BanII G_rGCy'C
Cuts at: 0 564 3774
Size: 564 3210
BbsI GAAGACnn'nnnn_
Cuts at: 0 1411 3290 3774
Size: 1411 1879 484
Fragments arranged by size:
1879 1411 484
BbvCI CC'TCA_GC
Cuts at: 0 803 3774
Size: 803 2971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1794 1828 3774
Size: 1794 34 1946
Fragments arranged by size:
1946 1794 34
BglII A'GATC_T
Cuts at: 0 2011 3162 3774
Size: 2011 1151 612
Fragments arranged by size:
2011 1151 612
Bme1580I G_kGCm'C
Cuts at: 0 3477 3774
Size: 3477 297
BmtI G_CTAG'C
Cuts at: 0 1579 3774
Size: 1579 2195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1073 1463 2564 3598 3774
Size: 1016 57 390 1101 1034 176
Fragments arranged by size:
1101 1034 1016 390 176 57
Bpu10I CC'TnA_GC
Cuts at: 0 803 3774
Size: 803 2971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 2150 2984 3774
Size: 96 2054 834 790
Fragments arranged by size:
2054 834 790 96
BsaAI yAC'GTr
Cuts at: 0 1843 3774
Size: 1843 1931
BsaBI GATnn'nnATC
Cuts at: 0 3455 3774
Size: 3455 319
BsaWI w'CCGG_w
Cuts at: 0 2946 3774
Size: 2946 828
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1281 1311 3774
Size: 1281 30 2463
Fragments arranged by size:
2463 1281 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 791 3774
Size: 791 2983
BseYI C'CCAG_C
Cuts at: 0 3460 3774
Size: 3460 314
BsmI GAATG_Cn'
Cuts at: 0 3538 3774
Size: 3538 236
Bsp1286I G_dGCh'C
Cuts at: 0 564 3477 3774
Size: 564 2913 297
Fragments arranged by size:
2913 564 297
BspHI T'CATG_A
Cuts at: 0 2220 3636 3774
Size: 2220 1416 138
Fragments arranged by size:
2220 1416 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1465 3774
Size: 1465 2309
BsrGI T'GTAC_A
Cuts at: 0 327 354 589 3774
Size: 327 27 235 3185
Fragments arranged by size:
3185 327 235 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1846 3774
Size: 1846 1928
BstBI TT'CG_AA
Cuts at: 0 841 3774
Size: 841 2933
BstYI r'GATC_y
Cuts at: 0 258 652 2011 2389 3162 3774
Size: 258 394 1359 378 773 612
Fragments arranged by size:
1359 773 612 394 378 258
BtsI GCAGTG_nn'
Cuts at: 0 1000 1287 3471 3774
Size: 1000 287 2184 303
Fragments arranged by size:
2184 1000 303 287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1719 1754 3774
Size: 1719 35 2020
Fragments arranged by size:
2020 1719 35
DraI TTT'AAA
Cuts at: 0 717 3774
Size: 717 3057
EarI CTCTTCn'nnn_
Cuts at: 0 1033 1598 1781 1910 2084 2394 3380 3774
Size: 1033 565 183 129 174 310 986 394
Fragments arranged by size:
1033 986 565 394 310 183 174 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 866 1016 1073 1463 1787 1843 2015
Size: 658 208 150 57 390 324 56 172
Cuts at: 2015 2069 2449 2564 2861 3302 3598 3774
Size: 54 380 115 297 441 296 176
Fragments arranged by size:
658 441 390 380 324 297 296 208
176 172 150 115 57 56 54
EcoO109I rG'GnC_Cy
Cuts at: 0 494 2380 3268 3774
Size: 494 1886 888 506
Fragments arranged by size:
1886 888 506 494
EcoRI G'AATT_C
Cuts at: 0 1433 3084 3774
Size: 1433 1651 690
Fragments arranged by size:
1651 1433 690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 73 896 1750 3105 3244 3774
Size: 73 823 854 1355 139 530
Fragments arranged by size:
1355 854 823 530 139 73
HaeII r_GCGC'y
Cuts at: 0 1716 3774
Size: 1716 2058
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 724 728 756 760 1281 1313
Size: 373 32 319 4 28 4 521 32
Cuts at: 1313 2604 2636 2836 2868 3558 3590 3774
Size: 1291 32 200 32 690 32 184
Fragments arranged by size:
1291 690 521 373 319 200 184 32
32 32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 0 747 2293 2318 3774
Size: 747 1546 25 1456
Fragments arranged by size:
1546 1456 747 25
HpaI GTT'AAC
Cuts at: 0 2318 3774
Size: 2318 1456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1172 2443 3774
Size: 1172 1271 1331
Fragments arranged by size:
1331 1271 1172
MslI CAynn'nnrTG
Cuts at: 0 235 356 1337 1835 2225 3641 3713 3774
Size: 235 121 981 498 390 1416 72 61
Fragments arranged by size:
1416 981 498 390 235 121 72 61
NdeI CA'TA_TG
Cuts at: 0 1661 3774
Size: 1661 2113
NheI G'CTAG_C
Cuts at: 0 1575 3774
Size: 1575 2199
NspI r_CATG'y
Cuts at: 0 355 604 3152 3774
Size: 355 249 2548 622
Fragments arranged by size:
2548 622 355 249
PciI A'CATG_T
Cuts at: 0 351 3148 3774
Size: 351 2797 626
Fragments arranged by size:
2797 626 351
PflMI CCAn_nnn'nTGG
Cuts at: 0 2967 3774
Size: 2967 807
PfoI T'CCnGG_A
Cuts at: 0 1452 3774
Size: 1452 2322
PmlI CAC'GTG
Cuts at: 0 1843 3774
Size: 1843 1931
PpuMI rG'GwC_Cy
Cuts at: 0 494 2380 3268 3774
Size: 494 1886 888 506
Fragments arranged by size:
1886 888 506 494
PspXI vC'TCGA_Gb
Cuts at: 0 1646 3774
Size: 1646 2128
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3197 3229 3774
Size: 3197 32 545
Fragments arranged by size:
3197 545 32
PstI C_TGCA'G
Cuts at: 0 1476 1616 3329 3774
Size: 1476 140 1713 445
Fragments arranged by size:
1713 1476 445 140
SanDI GG'GwC_CC
Cuts at: 0 494 3774
Size: 494 3280
SapI GCTCTTCn'nnn_
Cuts at: 0 1910 2394 3774
Size: 1910 484 1380
Fragments arranged by size:
1910 1380 484
ScaI AGT'ACT
Cuts at: 0 465 2859 3774
Size: 465 2394 915
Fragments arranged by size:
2394 915 465
SexAI A'CCwGG_T
Cuts at: 0 629 3360 3774
Size: 629 2731 414
Fragments arranged by size:
2731 629 414
SfcI C'TryA_G
Cuts at: 0 19 68 467 1292 1472 1580 1612 1696
Size: 19 49 399 825 180 108 32 84
Cuts at: 1696 3325 3774
Size: 1629 449
Fragments arranged by size:
1629 825 449 399 180 108 84 49
32 19
SmaI CCC'GGG
Cuts at: 0 2077 3774
Size: 2077 1697
SmlI C'TyrA_G
Cuts at: 0 111 1316 1646 2165 2853 2999 3774
Size: 111 1205 330 519 688 146 775
Fragments arranged by size:
1205 775 688 519 330 146 111
SspI AAT'ATT
Cuts at: 0 186 3657 3687 3774
Size: 186 3471 30 87
Fragments arranged by size:
3471 186 87 30
StuI AGG'CCT
Cuts at: 0 3331 3774
Size: 3331 443
StyI C'CwwG_G
Cuts at: 0 727 3774
Size: 727 3047
TatI w'GTAC_w
Cuts at: 0 327 354 423 463 589 1851 2525 2556
Size: 327 27 69 40 126 1262 674 31
Cuts at: 2556 2857 3774
Size: 301 917
Fragments arranged by size:
1262 917 674 327 301 126 69 40
31 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2402 3774
Size: 2402 1372
XhoI C'TCGA_G
Cuts at: 0 1316 1646 2853 3774
Size: 1316 330 1207 921
Fragments arranged by size:
1316 1207 921 330
XmaI C'CCGG_G
Cuts at: 0 2075 3774
Size: 2075 1699
XmnI GAAnn'nnTTC
Cuts at: 0 791 2135 3774
Size: 791 1344 1639
Fragments arranged by size:
1639 1344 791
Enzymes that do cut:
AcuI AflIII AhdI AlfI AloI AlwNI ApoI AvaI
AvrII BanII BbsI BbvCI BcgI BglII Bme1580I BmtI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsmI Bsp1286I BspHI BspMI BsrGI BstAPI BstBI
BstYI BtsI CspCI DraI EarI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII HpaI MmeI MslI NdeI
NheI NspI PciI PflMI PfoI PmlI PpuMI PspXI
PsrI PstI SanDI SapI ScaI SexAI SfcI SmaI
SmlI SspI StuI StyI TatI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI ApaI ApaLI AscI AseI AsiSI BaeI BamHI
BanI BbeI BciVI BclI BfrBI BglI BlpI BmgBI
BmrI BplI BsaI BsaHI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI HindIII KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI
PasI PmeI PpiI PshAI PsiI PspOMI PvuI PvuII
RsrII SacI SacII SalI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI TaqII TstI
Tth111I XbaI ZraI