Restriction map for
pF1KSDA1896
(Linear) (Six-base) MAPSORT of: KIAA1896.seq Check: 4950 from: 1 to: 1503
KIAA1896.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 1163 1463 1503
Size: 1163 300 40
Fragments arranged by size:
1163 300 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1495 1503
Size: 1027 468 8
Fragments arranged by size:
1027 468 8
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 761 1214 1503
Size: 761 453 289
Fragments arranged by size:
761 453 289
AlwNI CAG_nnn'CTG
Cuts at: 0 12 103 378 701 1262 1503
Size: 12 91 275 323 561 241
Fragments arranged by size:
561 323 275 241 91 12
ApaI G_GGCC'C
Cuts at: 0 460 1503
Size: 460 1043
ApoI r'AATT_y
Cuts at: 0 271 422 874 1503
Size: 271 151 452 629
Fragments arranged by size:
629 452 271 151
AvaI C'yCGr_G
Cuts at: 0 550 1503
Size: 550 953
BamHI G'GATC_C
Cuts at: 0 1070 1503
Size: 1070 433
BanI G'GyrC_C
Cuts at: 0 50 91 1246 1503
Size: 50 41 1155 257
Fragments arranged by size:
1155 257 50 41
BanII G_rGCy'C
Cuts at: 0 460 1503
Size: 460 1043
BbsI GAAGACnn'nnnn_
Cuts at: 0 1015 1036 1503
Size: 1015 21 467
Fragments arranged by size:
1015 467 21
BbvCI CC'TCA_GC
Cuts at: 0 155 307 1503
Size: 155 152 1196
Fragments arranged by size:
1196 155 152
BciVI GTATCCnnnnn_n'
Cuts at: 0 702 1503
Size: 702 801
Bme1580I G_kGCm'C
Cuts at: 0 460 1503
Size: 460 1043
BmtI G_CTAG'C
Cuts at: 0 32 1503
Size: 32 1471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 129 254 1503
Size: 45 84 125 1249
Fragments arranged by size:
1249 125 84 45
Bpu10I CC'TnA_GC
Cuts at: 0 155 307 1503
Size: 155 152 1196
Fragments arranged by size:
1196 155 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 709 1159 1503
Size: 412 297 450 344
Fragments arranged by size:
450 412 344 297
BsaI GGTCTCn'nnnn_
Cuts at: 0 107 1503
Size: 107 1396
BsaAI yAC'GTr
Cuts at: 0 1458 1503
Size: 1458 45
BsaBI GATnn'nnATC
Cuts at: 0 493 1503
Size: 493 1010
BsaWI w'CCGG_w
Cuts at: 0 1338 1503
Size: 1338 165
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 646 1503
Size: 517 129 857
Fragments arranged by size:
857 517 129
BseYI C'CCAG_C
Cuts at: 0 46 96 1125 1437 1503
Size: 46 50 1029 312 66
Fragments arranged by size:
1029 312 66 50 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1503
Size: 687 816
BsiEI CG_ry'CG
Cuts at: 0 78 1503
Size: 78 1425
BsiHKAI G_wGCw'C
Cuts at: 0 734 1235 1503
Size: 734 501 268
Fragments arranged by size:
734 501 268
BsmI GAATG_Cn'
Cuts at: 0 573 1055 1185 1503
Size: 573 482 130 318
Fragments arranged by size:
573 482 318 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1162 1503
Size: 1162 341
Bsp1286I G_dGCh'C
Cuts at: 0 460 734 1235 1503
Size: 460 274 501 268
Fragments arranged by size:
501 460 274 268
BspEI T'CCGG_A
Cuts at: 0 1338 1503
Size: 1338 165
BspHI T'CATG_A
Cuts at: 0 294 1424 1503
Size: 294 1130 79
Fragments arranged by size:
1130 294 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 207 709 729 1503
Size: 207 502 20 774
Fragments arranged by size:
774 502 207 20
BsrDI GCAATG_nn'
Cuts at: 0 837 1503
Size: 837 666
BsrFI r'CCGG_y
Cuts at: 0 1144 1503
Size: 1144 359
BsrGI T'GTAC_A
Cuts at: 0 225 1400 1503
Size: 225 1175 103
Fragments arranged by size:
1175 225 103
BssSI C'ACGA_G
Cuts at: 0 937 1503
Size: 937 566
BstAPI GCAn_nnn'nTGC
Cuts at: 0 378 1503
Size: 378 1125
BstEII G'GTnAC_C
Cuts at: 0 1344 1503
Size: 1344 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 1125 1503
Size: 186 939 378
Fragments arranged by size:
939 378 186
BstYI r'GATC_y
Cuts at: 0 1070 1503
Size: 1070 433
BtgI C'CryG_G
Cuts at: 0 54 161 536 1503
Size: 54 107 375 967
Fragments arranged by size:
967 375 107 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 957 1503
Size: 957 546
BtsI GCAGTG_nn'
Cuts at: 0 1208 1503
Size: 1208 295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 567 602 1503
Size: 567 35 901
Fragments arranged by size:
901 567 35
DraIII CAC_nnn'GTG
Cuts at: 0 537 666 1503
Size: 537 129 837
Fragments arranged by size:
837 537 129
DrdI GACnn_nn'nnGTC
Cuts at: 0 391 1159 1503
Size: 391 768 344
Fragments arranged by size:
768 391 344
EaeI y'GGCC_r
Cuts at: 0 1076 1091 1266 1274 1503
Size: 1076 15 175 8 229
Fragments arranged by size:
1076 229 175 15 8
EarI CTCTTCn'nnn_
Cuts at: 0 1366 1503
Size: 1366 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 129 254 1027 1495 1503
Size: 45 84 125 773 468 8
Fragments arranged by size:
773 468 125 84 45 8
EcoO109I rG'GnC_Cy
Cuts at: 0 243 456 457 956 1002 1301 1388 1503
Size: 243 213 1 499 46 299 87 115
Fragments arranged by size:
499 299 243 213 115 87 46 1
EcoRV GAT'ATC
Cuts at: 0 401 1503
Size: 401 1102
HaeII r_GCGC'y
Cuts at: 0 903 1024 1337 1503
Size: 903 121 313 166
Fragments arranged by size:
903 313 166 121
HindIII A'AGCT_T
Cuts at: 0 126 1503
Size: 126 1377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 1503
Size: 174 1329
MscI TGG'CCA
Cuts at: 0 1078 1268 1276 1503
Size: 1078 190 8 227
Fragments arranged by size:
1078 227 190 8
MslI CAynn'nnrTG
Cuts at: 0 469 790 1503
Size: 469 321 713
Fragments arranged by size:
713 469 321
MspA1I CmG'CkG
Cuts at: 0 1272 1441 1503
Size: 1272 169 62
Fragments arranged by size:
1272 169 62
NaeI GCC'GGC
Cuts at: 0 1146 1503
Size: 1146 357
NgoMIV G'CCGG_C
Cuts at: 0 1144 1503
Size: 1144 359
NheI G'CTAG_C
Cuts at: 0 28 1503
Size: 28 1475
NspI r_CATG'y
Cuts at: 0 1125 1503
Size: 1125 378
PasI CC'CwG_GG
Cuts at: 0 41 1485 1503
Size: 41 1444 18
Fragments arranged by size:
1444 41 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 500 537 1503
Size: 500 37 966
Fragments arranged by size:
966 500 37
PfoI T'CCnGG_A
Cuts at: 0 611 1503
Size: 611 892
PpuMI rG'GwC_Cy
Cuts at: 0 243 956 1002 1301 1503
Size: 243 713 46 299 202
Fragments arranged by size:
713 299 243 202 46
PspOMI G'GGCC_C
Cuts at: 0 456 1503
Size: 456 1047
PstI C_TGCA'G
Cuts at: 0 204 1193 1503
Size: 204 989 310
Fragments arranged by size:
989 310 204
PvuI CG_AT'CG
Cuts at: 0 78 1503
Size: 78 1425
PvuII CAG'CTG
Cuts at: 0 1272 1441 1503
Size: 1272 169 62
Fragments arranged by size:
1272 169 62
RsrII CG'GwC_CG
Cuts at: 0 1379 1503
Size: 1379 124
ScaI AGT'ACT
Cuts at: 0 1042 1503
Size: 1042 461
SexAI A'CCwGG_T
Cuts at: 0 662 1503
Size: 662 841
SfcI C'TryA_G
Cuts at: 0 200 1189 1503
Size: 200 989 314
Fragments arranged by size:
989 314 200
SmlI C'TyrA_G
Cuts at: 0 427 724 1174 1503
Size: 427 297 450 329
Fragments arranged by size:
450 427 329 297
StyI C'CwwG_G
Cuts at: 0 959 1503
Size: 959 544
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 38 583 1396 1503
Size: 38 545 813 107
Fragments arranged by size:
813 545 107 38
TatI w'GTAC_w
Cuts at: 0 225 1040 1400 1503
Size: 225 815 360 103
Fragments arranged by size:
815 360 225 103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 104 183 186 876 1071 1086 1284 1503
Size: 104 79 3 690 195 15 198 219
Fragments arranged by size:
690 219 198 195 104 79 15 3
XmnI GAAnn'nnTTC
Cuts at: 0 422 1503
Size: 422 1081
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BciVI Bme1580I BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI Eco57MI EcoO109I EcoRV HaeII HindIII MmeI MscI
MslI MspA1I NaeI NgoMIV NheI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuI PvuII RsrII ScaI
SexAI SfcI SmlI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BplI BsaHI BsaXI BsiWI BsrBI BssHII
BstBI BstZ17I Bsu36I ClaI DraI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI