Restriction map for
pF1KA1893
(Linear) (Six-base) MAPSORT of: KIAA1893.seq Check: 8081 from: 1 to: 2394
KIAA1893.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 676 2394
Size: 676 1718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1172 1220 1433 1457 2394
Size: 32 1140 48 213 24 937
Fragments arranged by size:
1140 937 213 48 32 24
AhdI GACnn_n'nnGTC
Cuts at: 0 1374 1508 1707 2394
Size: 1374 134 199 687
Fragments arranged by size:
1374 687 199 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1099 1131 2394
Size: 1099 32 1263
Fragments arranged by size:
1263 1099 32
AlwNI CAG_nnn'CTG
Cuts at: 0 391 559 1348 1681 1966 2394
Size: 391 168 789 333 285 428
Fragments arranged by size:
789 428 391 333 285 168
ApaI G_GGCC'C
Cuts at: 0 878 2394
Size: 878 1516
AvaI C'yCGr_G
Cuts at: 0 879 1758 1878 2212 2349 2394
Size: 879 879 120 334 137 45
Fragments arranged by size:
879 879 334 137 120 45
BamHI G'GATC_C
Cuts at: 0 507 579 603 634 699 723 1035 1224
Size: 507 72 24 31 65 24 312 189
Cuts at: 1224 1248 1770 1866 2010 2017 2394
Size: 24 522 96 144 7 377
Fragments arranged by size:
522 507 377 312 189 144 96 72
65 31 24 24 24 7
BanI G'GyrC_C
Cuts at: 0 1752 2218 2394
Size: 1752 466 176
Fragments arranged by size:
1752 466 176
BanII G_rGCy'C
Cuts at: 0 416 776 878 950 1014 1301 1634 2039
Size: 416 360 102 72 64 287 333 405
Cuts at: 2039 2394
Size: 355
Fragments arranged by size:
416 405 360 355 333 287 102 72
64
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 1226 1439 1463 2155 2394
Size: 20 1206 213 24 692 239
Fragments arranged by size:
1206 692 239 213 24 20
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2175 2209 2312 2346 2394
Size: 2175 34 103 34 48
Fragments arranged by size:
2175 103 48 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1005 1170 1362 2394
Size: 1005 165 192 1032
Fragments arranged by size:
1032 1005 192 165
BclI T'GATC_A
Cuts at: 0 2381 2394
Size: 2381 13
BglII A'GATC_T
Cuts at: 0 627 843 1200 2394
Size: 627 216 357 1194
Fragments arranged by size:
1194 627 357 216
BlpI GC'TnA_GC
Cuts at: 0 1532 2199 2394
Size: 1532 667 195
Fragments arranged by size:
1532 667 195
Bme1580I G_kGCm'C
Cuts at: 0 878 902 1757 2394
Size: 878 24 855 637
Fragments arranged by size:
878 855 637 24
BmgBI CAC'GTC
Cuts at: 0 374 2394
Size: 374 2020
BmrI ACTGGGnnnn_n'
Cuts at: 0 215 2394
Size: 215 2179
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 330 2394
Size: 330 2064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 648 1365 1718 2154 2164 2394
Size: 13 635 717 353 436 10 230
Fragments arranged by size:
717 635 436 353 230 13 10
Bpu10I CC'TnA_GC
Cuts at: 0 1284 2394
Size: 1284 1110
BsaI GGTCTCn'nnnn_
Cuts at: 0 1731 2213 2394
Size: 1731 482 181
Fragments arranged by size:
1731 482 181
BsaHI Gr'CG_yC
Cuts at: 0 1155 2394
Size: 1155 1239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 763 793 996 1026 1123 1153
Size: 124 30 609 30 203 30 97 30
Cuts at: 1153 2048 2078 2394
Size: 895 30 316
Fragments arranged by size:
895 609 316 203 124 97 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 1131 2154 2157 2394
Size: 784 347 1023 3 237
Fragments arranged by size:
1023 784 347 237 3
BseYI C'CCAG_C
Cuts at: 0 143 229 2394
Size: 143 86 2165
Fragments arranged by size:
2165 143 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 2072 2394
Size: 146 1926 322
Fragments arranged by size:
1926 322 146
BsiEI CG_ry'CG
Cuts at: 0 2291 2322 2394
Size: 2291 31 72
Fragments arranged by size:
2291 72 31
BsiHKAI G_wGCw'C
Cuts at: 0 664 1285 1301 2394
Size: 664 621 16 1093
Fragments arranged by size:
1093 664 621 16
Bsp1286I G_dGCh'C
Cuts at: 0 416 664 776 878 902 950 1014 1285
Size: 416 248 112 102 24 48 64 271
Cuts at: 1285 1301 1634 1757 2039 2394
Size: 16 333 123 282 355
Fragments arranged by size:
416 355 333 282 271 248 123 112
102 64 48 24 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 298 1498 1570 2394
Size: 298 1200 72 824
Fragments arranged by size:
1200 824 298 72
BsrFI r'CCGG_y
Cuts at: 0 2286 2394
Size: 2286 108
BssHII G'CGCG_C
Cuts at: 0 2265 2394
Size: 2265 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 160 2281 2394
Size: 160 2121 113
Fragments arranged by size:
2121 160 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1047 1497 2394
Size: 1047 450 897
Fragments arranged by size:
1047 897 450
BstYI r'GATC_y
Cuts at: 0 507 531 579 603 627 634 651 699
Size: 507 24 48 24 24 7 17 48
Cuts at: 699 723 843 867 891 1035 1059 1200 1224
Size: 24 120 24 24 144 24 141 24
Cuts at: 1224 1248 1557 1770 1866 2010 2017 2394
Size: 24 309 213 96 144 7 377
Fragments arranged by size:
507 377 309 213 144 144 141 120
96 48 48 24 24 24 24 24
24 24 24 24 17 7 7
BstZ17I GTA'TAC
Cuts at: 0 677 2394
Size: 677 1717
Bsu36I CC'TnA_GG
Cuts at: 0 1143 1617 2046 2394
Size: 1143 474 429 348
Fragments arranged by size:
1143 474 429 348
BtgI C'CryG_G
Cuts at: 0 2291 2316 2394
Size: 2291 25 78
Fragments arranged by size:
2291 78 25
BtsI GCAGTG_nn'
Cuts at: 0 5 2235 2394
Size: 5 2230 159
Fragments arranged by size:
2230 159 5
DrdI GACnn_nn'nnGTC
Cuts at: 0 2157 2394
Size: 2157 237
EaeI y'GGCC_r
Cuts at: 0 1167 2288 2319 2394
Size: 1167 1121 31 75
Fragments arranged by size:
1167 1121 75 31
EagI C'GGCC_G
Cuts at: 0 2288 2319 2394
Size: 2288 31 75
Fragments arranged by size:
2288 75 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1879 2238 2394
Size: 1879 359 156
Fragments arranged by size:
1879 359 156
EcoICRI GAG'CTC
Cuts at: 0 1299 2394
Size: 1299 1095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 32 648 1172 1220 1365 1433 1457
Size: 13 19 616 524 48 145 68 24
Cuts at: 1457 1718 2154 2164 2394
Size: 261 436 10 230
Fragments arranged by size:
616 524 436 261 230 145 68 48
24 19 13 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1026 1383 2169 2394
Size: 1026 357 786 225
Fragments arranged by size:
1026 786 357 225
EcoO109I rG'GnC_Cy
Cuts at: 0 61 239 515 875 1019 1368 1392 1493
Size: 61 178 276 360 144 349 24 101
Cuts at: 1493 1510 1653 1874 2312 2389 2394
Size: 17 143 221 438 77 5
Fragments arranged by size:
438 360 349 276 221 178 144 143
101 77 61 24 17 5
HaeII r_GCGC'y
Cuts at: 0 1943 2394
Size: 1943 451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 332 364 379 403 411 435 451 483
Size: 332 32 15 24 8 24 16 32
Cuts at: 483 499 531 619 651 691 715 723 747
Size: 16 32 88 32 40 24 8 24
Cuts at: 747 907 939 996 1028 1044 1051 1076 1083
Size: 160 32 57 32 16 7 25 7
Cuts at: 1083 1123 1155 1192 1216 1224 1248 1288 1312
Size: 40 32 37 24 8 24 40 24
Cuts at: 1312 1320 1344 1360 1384 1392 1416 1477 1494
Size: 8 24 16 24 8 24 61 17
Cuts at: 1494 1509 1526 1549 1581 1693 1725 1762 1794
Size: 15 17 23 32 112 32 37 32
Cuts at: 1794 2002 2034 2050 2082 2098 2130 2394
Size: 208 32 16 32 16 32 264
Fragments arranged by size:
332 264 208 160 112 88 61 57
40 40 40 37 37 32 32 32
32 32 32 32 32 32 32 32
32 32 25 24 24 24 24 24
24 24 24 24 24 23 17 17
16 16 16 16 16 16 15 15
8 8 8 8 8 7 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2382 2394
Size: 2382 12
MscI TGG'CCA
Cuts at: 0 1169 2394
Size: 1169 1225
MspA1I CmG'CkG
Cuts at: 0 33 2293 2394
Size: 33 2260 101
Fragments arranged by size:
2260 101 33
NaeI GCC'GGC
Cuts at: 0 2288 2394
Size: 2288 106
NgoMIV G'CCGG_C
Cuts at: 0 2286 2394
Size: 2286 108
NspI r_CATG'y
Cuts at: 0 1046 2394
Size: 1046 1348
PasI CC'CwG_GG
Cuts at: 0 639 1782 1871 1927 2070 2394
Size: 639 1143 89 56 143 324
Fragments arranged by size:
1143 639 324 143 89 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 94 2394
Size: 94 2300
PfoI T'CCnGG_A
Cuts at: 0 591 1308 1734 2013 2394
Size: 591 717 426 279 381
Fragments arranged by size:
717 591 426 381 279
PpuMI rG'GwC_Cy
Cuts at: 0 61 515 1019 1368 1392 1493 1510 1653
Size: 61 454 504 349 24 101 17 143
Cuts at: 1653 2312 2394
Size: 659 82
Fragments arranged by size:
659 504 454 349 143 101 82 61
24 17
PspOMI G'GGCC_C
Cuts at: 0 874 2394
Size: 874 1520
PstI C_TGCA'G
Cuts at: 0 834 1524 1947 2026 2277 2394
Size: 834 690 423 79 251 117
Fragments arranged by size:
834 690 423 251 117 79
PvuII CAG'CTG
Cuts at: 0 33 2394
Size: 33 2361
SacI G_AGCT'C
Cuts at: 0 1301 2394
Size: 1301 1093
SacII CC_GC'GG
Cuts at: 0 2294 2394
Size: 2294 100
SanDI GG'GwC_CC
Cuts at: 0 2312 2394
Size: 2312 82
SfcI C'TryA_G
Cuts at: 0 830 1520 1712 1943 2022 2273 2394
Size: 830 690 192 231 79 251 121
Fragments arranged by size:
830 690 251 231 192 121 79
StuI AGG'CCT
Cuts at: 0 1996 2394
Size: 1996 398
StyI C'CwwG_G
Cuts at: 0 317 902 1022 1496 2117 2394
Size: 317 585 120 474 621 277
Fragments arranged by size:
621 585 474 317 277 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 2048 2080 2394
Size: 1264 32 752 32 314
Fragments arranged by size:
1264 752 314 32 32
XbaI T'CTAG_A
Cuts at: 0 205 1830 2394
Size: 205 1625 564
Fragments arranged by size:
1625 564 205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 324 2394
Size: 267 57 2070
Fragments arranged by size:
2070 267 57
Enzymes that do cut:
AccI AcuI AhdI AloI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssHII BstAPI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MscI MspA1I NaeI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SfcI StuI StyI TstI XbaI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BfrBI BglI BmtI BpuEI BsaAI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssSI BstBI BstEII BtgZI ClaI CspCI
DraI DraIII EarI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI Tth111I XhoI
XmaI XmnI ZraI