Restriction map for
pF1KSDA1892
(Linear) (Six-base) MAPSORT of: KIAA1892.seq Check: 1119 from: 1 to: 1359
KIAA1892.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 140 1359
Size: 140 1219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1307 1359
Size: 256 1051 52
Fragments arranged by size:
1051 256 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 224 1359
Size: 224 1135
AlwNI CAG_nnn'CTG
Cuts at: 0 598 1359
Size: 598 761
ApaI G_GGCC'C
Cuts at: 0 73 1359
Size: 73 1286
AvaI C'yCGr_G
Cuts at: 0 191 1359
Size: 191 1168
BamHI G'GATC_C
Cuts at: 0 519 966 1359
Size: 519 447 393
Fragments arranged by size:
519 447 393
BanII G_rGCy'C
Cuts at: 0 73 771 943 1081 1107 1331 1352 1359
Size: 73 698 172 138 26 224 21 7
Fragments arranged by size:
698 224 172 138 73 26 21 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 743 1359
Size: 743 616
BciVI GTATCCnnnnn_n'
Cuts at: 0 436 1359
Size: 436 923
Bme1580I G_kGCm'C
Cuts at: 0 73 1359
Size: 73 1286
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 808 1359
Size: 221 587 551
Fragments arranged by size:
587 551 221
BmtI G_CTAG'C
Cuts at: 0 466 1359
Size: 466 893
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 80 1146 1333 1359
Size: 80 1066 187 26
Fragments arranged by size:
1066 187 80 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 491 1239 1359
Size: 419 72 748 120
Fragments arranged by size:
748 419 120 72
Bpu10I CC'TnA_GC
Cuts at: 0 65 1359
Size: 65 1294
BsaAI yAC'GTr
Cuts at: 0 607 1359
Size: 607 752
BsaWI w'CCGG_w
Cuts at: 0 1028 1359
Size: 1028 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 1359
Size: 232 30 1097
Fragments arranged by size:
1097 232 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 488 853 1359
Size: 488 365 506
Fragments arranged by size:
506 488 365
BseYI C'CCAG_C
Cuts at: 0 52 83 410 1345 1359
Size: 52 31 327 935 14
Fragments arranged by size:
935 327 52 31 14
BsiHKAI G_wGCw'C
Cuts at: 0 1052 1107 1359
Size: 1052 55 252
Fragments arranged by size:
1052 252 55
Bsp1286I G_dGCh'C
Cuts at: 0 73 771 943 1052 1081 1107 1331 1352
Size: 73 698 172 109 29 26 224 21
Cuts at: 1352 1359
Size: 7
Fragments arranged by size:
698 224 172 109 73 29 26 21
7
BspHI T'CATG_A
Cuts at: 0 1206 1359
Size: 1206 153
BstAPI GCAn_nnn'nTGC
Cuts at: 0 323 1359
Size: 323 1036
BstYI r'GATC_y
Cuts at: 0 130 371 519 557 966 1359
Size: 130 241 148 38 409 393
Fragments arranged by size:
409 393 241 148 130 38
BstZ17I GTA'TAC
Cuts at: 0 141 1359
Size: 141 1218
BtgI C'CryG_G
Cuts at: 0 970 1332 1359
Size: 970 362 27
Fragments arranged by size:
970 362 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 552 1359
Size: 552 807
BtsI GCAGTG_nn'
Cuts at: 0 457 599 812 941 1026 1269 1359
Size: 457 142 213 129 85 243 90
Fragments arranged by size:
457 243 213 142 129 90 85
DraIII CAC_nnn'GTG
Cuts at: 0 675 1359
Size: 675 684
EarI CTCTTCn'nnn_
Cuts at: 0 121 1115 1359
Size: 121 994 244
Fragments arranged by size:
994 244 121
EcoICRI GAG'CTC
Cuts at: 0 1105 1359
Size: 1105 254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 419 491 1239 1307 1359
Size: 256 163 72 748 68 52
Fragments arranged by size:
748 256 163 72 68 52
EcoO109I rG'GnC_Cy
Cuts at: 0 69 260 684 1312 1359
Size: 69 191 424 628 47
Fragments arranged by size:
628 424 191 69 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 1359
Size: 150 1209
HaeII r_GCGC'y
Cuts at: 0 37 1359
Size: 37 1322
MslI CAynn'nnrTG
Cuts at: 0 206 285 1060 1359
Size: 206 79 775 299
Fragments arranged by size:
775 299 206 79
NcoI C'CATG_G
Cuts at: 0 1332 1359
Size: 1332 27
NheI G'CTAG_C
Cuts at: 0 462 1359
Size: 462 897
PflMI CCAn_nnn'nTGG
Cuts at: 0 572 1359
Size: 572 787
PmlI CAC'GTG
Cuts at: 0 607 1359
Size: 607 752
PpuMI rG'GwC_Cy
Cuts at: 0 260 684 1312 1359
Size: 260 424 628 47
Fragments arranged by size:
628 424 260 47
PspOMI G'GGCC_C
Cuts at: 0 69 1359
Size: 69 1290
SacI G_AGCT'C
Cuts at: 0 1107 1359
Size: 1107 252
SanDI GG'GwC_CC
Cuts at: 0 260 684 1359
Size: 260 424 675
Fragments arranged by size:
675 424 260
SfcI C'TryA_G
Cuts at: 0 169 1359
Size: 169 1190
SmlI C'TyrA_G
Cuts at: 0 191 1359
Size: 191 1168
StuI AGG'CCT
Cuts at: 0 715 1359
Size: 715 644
StyI C'CwwG_G
Cuts at: 0 255 1332 1359
Size: 255 1077 27
Fragments arranged by size:
1077 255 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1282 1359
Size: 1282 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1359
Size: 511 32 816
Fragments arranged by size:
816 511 32
XbaI T'CTAG_A
Cuts at: 0 451 1359
Size: 451 908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 418 1359
Size: 418 941
XhoI C'TCGA_G
Cuts at: 0 191 1359
Size: 191 1168
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI AvaI BamHI BanII
BbsI BciVI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI
BstAPI BstYI BstZ17I BtgI BtgZI BtsI DraIII EarI
EcoICRI Eco57MI EcoO109I FalI HaeII MslI NcoI NheI
PflMI PmlI PpuMI PspOMI SacI SanDI SfcI SmlI
StuI StyI TaqII TstI XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BpuEI BsaI BsaBI BsaHI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
Bsu36I ClaI CspCI DraI DrdI EaeI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XmaI XmnI ZraI