Restriction map for
pF1KSDA1860
(Linear) (Six-base) MAPSORT of: KIAA1860.seq Check: 4800 from: 1 to: 2064
KIAA1860.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 588 1360 2064
Size: 565 23 772 704
Fragments arranged by size:
772 704 565 23
AflIII A'CryG_T
Cuts at: 0 638 833 2064
Size: 638 195 1231
Fragments arranged by size:
1231 638 195
AhdI GACnn_n'nnGTC
Cuts at: 0 1992 2064
Size: 1992 72
AlwNI CAG_nnn'CTG
Cuts at: 0 306 2064
Size: 306 1758
ApaI G_GGCC'C
Cuts at: 0 1772 2064
Size: 1772 292
ApaLI G'TGCA_C
Cuts at: 0 505 2064
Size: 505 1559
AvaI C'yCGr_G
Cuts at: 0 24 148 473 901 973 1551 2064
Size: 24 124 325 428 72 578 513
Fragments arranged by size:
578 513 428 325 124 72 24
BamHI G'GATC_C
Cuts at: 0 1945 2064
Size: 1945 119
BanI G'GyrC_C
Cuts at: 0 55 115 1132 1164 1204 1603 1614 1647
Size: 55 60 1017 32 40 399 11 33
Cuts at: 1647 1927 2064
Size: 280 137
Fragments arranged by size:
1017 399 280 137 60 55 40 33
32 11
BanII G_rGCy'C
Cuts at: 0 655 974 1772 2064
Size: 655 319 798 292
Fragments arranged by size:
798 655 319 292
BbeI G_GCGC'C
Cuts at: 0 1136 1168 1931 2064
Size: 1136 32 763 133
Fragments arranged by size:
1136 763 133 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 391 1114 2064
Size: 391 723 950
Fragments arranged by size:
950 723 391
BbvCI CC'TCA_GC
Cuts at: 0 184 2064
Size: 184 1880
BglI GCCn_nnn'nGGC
Cuts at: 0 1798 1818 2064
Size: 1798 20 246
Fragments arranged by size:
1798 246 20
BlpI GC'TnA_GC
Cuts at: 0 1559 2064
Size: 1559 505
Bme1580I G_kGCm'C
Cuts at: 0 120 509 776 1619 1772 2064
Size: 120 389 267 843 153 292
Fragments arranged by size:
843 389 292 267 153 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 121 2064
Size: 121 1943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 738 1398 1617 2064
Size: 440 298 660 219 447
Fragments arranged by size:
660 447 440 298 219
Bpu10I CC'TnA_GC
Cuts at: 0 184 1599 2064
Size: 184 1415 465
Fragments arranged by size:
1415 465 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 2064
Size: 766 1298
BsaAI yAC'GTr
Cuts at: 0 165 2064
Size: 165 1899
BsaBI GATnn'nnATC
Cuts at: 0 931 2064
Size: 931 1133
BsaHI Gr'CG_yC
Cuts at: 0 1133 1165 1928 2064
Size: 1133 32 763 136
Fragments arranged by size:
1133 763 136 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1380 1410 2064
Size: 1380 30 654
Fragments arranged by size:
1380 654 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 66 166 964 991 1129 1360 2064
Size: 66 100 798 27 138 231 704
Fragments arranged by size:
798 704 231 138 100 66 27
BseYI C'CCAG_C
Cuts at: 0 278 289 621 882 1098 1168 2064
Size: 278 11 332 261 216 70 896
Fragments arranged by size:
896 332 278 261 216 70 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1274 1282 1780 1787 2064
Size: 1274 8 498 7 277
Fragments arranged by size:
1274 498 277 8 7
BsiEI CG_ry'CG
Cuts at: 0 36 2064
Size: 36 2028
BsiHKAI G_wGCw'C
Cuts at: 0 509 1324 1489 2064
Size: 509 815 165 575
Fragments arranged by size:
815 575 509 165
Bsp1286I G_dGCh'C
Cuts at: 0 120 509 655 776 974 1324 1489 1619
Size: 120 389 146 121 198 350 165 130
Cuts at: 1619 1772 2064
Size: 153 292
Fragments arranged by size:
389 350 292 198 165 153 146 130
121 120
BspHI T'CATG_A
Cuts at: 0 326 911 2064
Size: 326 585 1153
Fragments arranged by size:
1153 585 326
BsrBI CCG'CTC
Cuts at: 0 74 107 1350 1467 1665 2064
Size: 74 33 1243 117 198 399
Fragments arranged by size:
1243 399 198 117 74 33
BsrFI r'CCGG_y
Cuts at: 0 1727 2064
Size: 1727 337
BsrGI T'GTAC_A
Cuts at: 0 531 737 2064
Size: 531 206 1327
Fragments arranged by size:
1327 531 206
BssSI C'ACGA_G
Cuts at: 0 439 1482 2064
Size: 439 1043 582
Fragments arranged by size:
1043 582 439
BstEII G'GTnAC_C
Cuts at: 0 1878 2064
Size: 1878 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1422 2064
Size: 1422 642
BstYI r'GATC_y
Cuts at: 0 1457 1945 2064
Size: 1457 488 119
Fragments arranged by size:
1457 488 119
Bsu36I CC'TnA_GG
Cuts at: 0 1990 2064
Size: 1990 74
BtgI C'CryG_G
Cuts at: 0 1382 1421 1609 1707 2064
Size: 1382 39 188 98 357
Fragments arranged by size:
1382 357 188 98 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 2064
Size: 120 1944
BtsI GCAGTG_nn'
Cuts at: 0 72 2064
Size: 72 1992
DraIII CAC_nnn'GTG
Cuts at: 0 115 2064
Size: 115 1949
EaeI y'GGCC_r
Cuts at: 0 69 450 1713 2064
Size: 69 381 1263 351
Fragments arranged by size:
1263 381 351 69
EarI CTCTTCn'nnn_
Cuts at: 0 1242 1849 2064
Size: 1242 607 215
Fragments arranged by size:
1242 607 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1348 1485 2064
Size: 1348 137 579
Fragments arranged by size:
1348 579 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 565 588 738 1360 1398 1617 2064
Size: 440 125 23 150 622 38 219 447
Fragments arranged by size:
622 447 440 219 150 125 38 23
EcoO109I rG'GnC_Cy
Cuts at: 0 333 540 822 1769 1986 2064
Size: 333 207 282 947 217 78
Fragments arranged by size:
947 333 282 217 207 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 222 2064
Size: 222 1842
HaeII r_GCGC'y
Cuts at: 0 1136 1168 1436 1566 1931 2064
Size: 1136 32 268 130 365 133
Fragments arranged by size:
1136 365 268 133 130 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 1380 1412 1552 1584 1937 1969
Size: 25 32 1323 32 140 32 353 32
Cuts at: 1969 1978 2010 2064
Size: 9 32 54
Fragments arranged by size:
1323 353 140 54 32 32 32 32
32 25 9
HincII GTy'rAC
Cuts at: 0 839 2064
Size: 839 1225
KasI G'GCGC_C
Cuts at: 0 1132 1164 1927 2064
Size: 1132 32 763 137
Fragments arranged by size:
1132 763 137 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 2064
Size: 510 1554
MscI TGG'CCA
Cuts at: 0 1715 2064
Size: 1715 349
MspA1I CmG'CkG
Cuts at: 0 282 752 1227 1384 1916 2064
Size: 282 470 475 157 532 148
Fragments arranged by size:
532 475 470 282 157 148
NarI GG'CG_CC
Cuts at: 0 1133 1165 1928 2064
Size: 1133 32 763 136
Fragments arranged by size:
1133 763 136 32
NcoI C'CATG_G
Cuts at: 0 1421 1707 2064
Size: 1421 286 357
Fragments arranged by size:
1421 357 286
NdeI CA'TA_TG
Cuts at: 0 660 2064
Size: 660 1404
NspI r_CATG'y
Cuts at: 0 837 2064
Size: 837 1227
PasI CC'CwG_GG
Cuts at: 0 1138 1941 2064
Size: 1138 803 123
Fragments arranged by size:
1138 803 123
PciI A'CATG_T
Cuts at: 0 833 2064
Size: 833 1231
PfoI T'CCnGG_A
Cuts at: 0 1721 2064
Size: 1721 343
PmlI CAC'GTG
Cuts at: 0 165 2064
Size: 165 1899
PpuMI rG'GwC_Cy
Cuts at: 0 540 822 1986 2064
Size: 540 282 1164 78
Fragments arranged by size:
1164 540 282 78
PspOMI G'GGCC_C
Cuts at: 0 1768 2064
Size: 1768 296
PspXI vC'TCGA_Gb
Cuts at: 0 473 2064
Size: 473 1591
PstI C_TGCA'G
Cuts at: 0 302 1378 2064
Size: 302 1076 686
Fragments arranged by size:
1076 686 302
PvuI CG_AT'CG
Cuts at: 0 36 2064
Size: 36 2028
PvuII CAG'CTG
Cuts at: 0 282 2064
Size: 282 1782
SacII CC_GC'GG
Cuts at: 0 1385 2064
Size: 1385 679
SanDI GG'GwC_CC
Cuts at: 0 822 2064
Size: 822 1242
ScaI AGT'ACT
Cuts at: 0 803 2064
Size: 803 1261
SexAI A'CCwGG_T
Cuts at: 0 395 2064
Size: 395 1669
SfcI C'TryA_G
Cuts at: 0 298 1374 2064
Size: 298 1076 690
Fragments arranged by size:
1076 690 298
SfoI GGC'GCC
Cuts at: 0 1134 1166 1929 2064
Size: 1134 32 763 135
Fragments arranged by size:
1134 763 135 32
SmaI CCC'GGG
Cuts at: 0 26 1553 2064
Size: 26 1527 511
Fragments arranged by size:
1527 511 26
SmlI C'TyrA_G
Cuts at: 0 473 781 901 2064
Size: 473 308 120 1163
Fragments arranged by size:
1163 473 308 120
SspI AAT'ATT
Cuts at: 0 528 2064
Size: 528 1536
StyI C'CwwG_G
Cuts at: 0 59 1421 1707 2013 2064
Size: 59 1362 286 306 51
Fragments arranged by size:
1362 306 286 59 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 2064
Size: 103 1961
TatI w'GTAC_w
Cuts at: 0 531 737 801 895 1067 2064
Size: 531 206 64 94 172 997
Fragments arranged by size:
997 531 206 172 94 64
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 219 1641 2064
Size: 108 111 1422 423
Fragments arranged by size:
1422 423 111 108
XhoI C'TCGA_G
Cuts at: 0 473 901 2064
Size: 473 428 1163
Fragments arranged by size:
1163 473 428
XmaI C'CCGG_G
Cuts at: 0 24 1551 2064
Size: 24 1527 513
Fragments arranged by size:
1527 513 24
XmnI GAAnn'nnTTC
Cuts at: 0 307 1191 2064
Size: 307 884 873
Fragments arranged by size:
884 873 307
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI
BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MspA1I
NarI NcoI NdeI NspI PasI PciI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuI PvuII SacII SanDI
ScaI SexAI SfcI SfoI SmaI SmlI SspI StyI
TaqII TatI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BsaI BsaWI BsiWI BsmI BsmBI BspEI BspMI
BsrDI BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MslI NaeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PpiI PshAI PsiI PsrI RsrII SacI SalI SapI
SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XbaI ZraI