(Linear) (Six-base) MAPSORT of: KIAA1830.seq  Check: 4364  from: 1  to: 1362



KIAA1830.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     497    1362 
   Size:        257     240     865

  Fragments arranged by size: 

                 865     257     240
 
AseI AT'TA_AT

Cuts at:      0    1007    1362 
   Size:       1007     355
 
BanII G_rGCy'C

Cuts at:      0     396    1120    1295    1334    1362 
   Size:        396     724     175      39      28

  Fragments arranged by size: 

                 724     396     175      39      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     995    1362 
   Size:        995     367
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1165    1362 
   Size:       1165     197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    1362 
   Size:        209    1153
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223    1362 
   Size:        223    1139
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     827    1362 
   Size:        827     535
 
BsaBI GATnn'nnATC

Cuts at:      0     650    1362 
   Size:        650     712
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1295    1325    1362 
   Size:       1295      30      37

  Fragments arranged by size: 

                1295      37      30
 
BseYI C'CCAG_C

Cuts at:      0    1294    1362 
   Size:       1294      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1345    1362 
   Size:       1345      17
 
BsiHKAI G_wGCw'C

Cuts at:      0    1120    1362 
   Size:       1120     242
 
Bsp1286I G_dGCh'C

Cuts at:      0     396    1120    1295    1334    1362 
   Size:        396     724     175      39      28

  Fragments arranged by size: 

                 724     396     175      39      28
 
BsrDI GCAATG_nn'

Cuts at:      0     266    1362 
   Size:        266    1096
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     871    1362 
   Size:        871     491
 
BstYI r'GATC_y

Cuts at:      0    1306    1362 
   Size:       1306      56
 
BtgI C'CryG_G

Cuts at:      0     151    1362 
   Size:        151    1211
 
BtsI GCAGTG_nn'

Cuts at:      0    1305    1362 
   Size:       1305      57
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      30      65    1362 
   Size:         30      35    1297

  Fragments arranged by size: 

                1297      35      30
 
DraI TTT'AAA

Cuts at:      0     105    1362 
   Size:        105    1257
 
DraIII CAC_nnn'GTG

Cuts at:      0    1159    1362 
   Size:       1159     203
 
EaeI y'GGCC_r

Cuts at:      0     159     403     861    1362 
   Size:        159     244     458     501

  Fragments arranged by size: 

                 501     458     244     159
 
EarI CTCTTCn'nnn_

Cuts at:      0       7     436     844    1265    1362 
   Size:          7     429     408     421      97

  Fragments arranged by size: 

                 429     421     408      97       7
 
EcoICRI GAG'CTC

Cuts at:      0    1118    1362 
   Size:       1118     244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     257     497    1362 
   Size:        209      48     240     865

  Fragments arranged by size: 

                 865     240     209      48
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     217    1362 
   Size:        217    1145
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     643     675    1075    1107    1362 
   Size:        643      32     400      32     255

  Fragments arranged by size: 

                 643     400     255      32      32
 
HincII GTy'rAC

Cuts at:      0     418    1362 
   Size:        418     944
 
HindIII A'AGCT_T

Cuts at:      0     739    1362 
   Size:        739     623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     216     322    1362 
   Size:        216     106    1040

  Fragments arranged by size: 

                1040     216     106
 
MscI TGG'CCA

Cuts at:      0     161     863    1362 
   Size:        161     702     499

  Fragments arranged by size: 

                 702     499     161
 
MslI CAynn'nnrTG

Cuts at:      0     869    1362 
   Size:        869     493
 
MspA1I CmG'CkG

Cuts at:      0     595    1362 
   Size:        595     767
 
NcoI C'CATG_G

Cuts at:      0     151    1362 
   Size:        151    1211
 
NdeI CA'TA_TG

Cuts at:      0     434    1362 
   Size:        434     928
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     157    1362 
   Size:        157    1205
 
PvuII CAG'CTG

Cuts at:      0     595    1362 
   Size:        595     767
 
SacI G_AGCT'C

Cuts at:      0    1120    1362 
   Size:       1120     242
 
SfcI C'TryA_G

Cuts at:      0     923    1310    1319    1362 
   Size:        923     387       9      43

  Fragments arranged by size: 

                 923     387      43       9
 
SmlI C'TyrA_G

Cuts at:      0     202    1362 
   Size:        202    1160
 
SspI AAT'ATT

Cuts at:      0     955    1362 
   Size:        955     407
 
StyI C'CwwG_G

Cuts at:      0     151    1038    1334    1362 
   Size:        151     887     296      28

  Fragments arranged by size: 

                 887     296     151      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1147    1362 
   Size:       1147     215
 
TatI w'GTAC_w

Cuts at:      0      51     930    1362 
   Size:         51     879     432

  Fragments arranged by size: 

                 879     432      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     728     760    1298    1330    1362 
   Size:        728      32     538      32      32

  Fragments arranged by size: 

                 728     538      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     994    1362 
   Size:        994     368
 
XmnI GAAnn'nnTTC

Cuts at:      0     560     680    1362 
   Size:        560     120     682

  Fragments arranged by size: 

                 682     560     120
 

 Enzymes that do cut:

     AcuI     AseI    BanII     BbsI     BmrI     BpmI    BpuEI     BsaI
    BsaBI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI    BstXI
    BstYI     BtgI     BtsI    CspCI     DraI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI     FalI    Hin4I   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI    PflMI    PvuII     SacI     SfcI
     SmlI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaHI
    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DrdI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     XbaI
     XcmI     XhoI     XmaI     ZraI