Restriction map for
pF1KSDA1798
(Linear) (Six-base) MAPSORT of: KIAA1798.seq Check: 6640 from: 1 to: 2340
KIAA1798.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 261 2340
Size: 261 2079
AccI GT'mk_AC
Cuts at: 0 1139 1451 2340
Size: 1139 312 889
Fragments arranged by size:
1139 889 312
Acc65I G'GTAC_C
Cuts at: 0 195 2340
Size: 195 2145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1603 1640 2340
Size: 334 1269 37 700
Fragments arranged by size:
1269 700 334 37
AflII C'TTAA_G
Cuts at: 0 1036 1797 2340
Size: 1036 761 543
Fragments arranged by size:
1036 761 543
AflIII A'CryG_T
Cuts at: 0 1042 2340
Size: 1042 1298
AhdI GACnn_n'nnGTC
Cuts at: 0 2136 2340
Size: 2136 204
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1970 2002 2340
Size: 1970 32 338
Fragments arranged by size:
1970 338 32
ApaI G_GGCC'C
Cuts at: 0 1729 2272 2340
Size: 1729 543 68
Fragments arranged by size:
1729 543 68
ApoI r'AATT_y
Cuts at: 0 1015 2143 2282 2322 2340
Size: 1015 1128 139 40 18
Fragments arranged by size:
1128 1015 139 40 18
AseI AT'TA_AT
Cuts at: 0 1645 2340
Size: 1645 695
AvaI C'yCGr_G
Cuts at: 0 228 823 1680 1970 2340
Size: 228 595 857 290 370
Fragments arranged by size:
857 595 370 290 228
BamHI G'GATC_C
Cuts at: 0 327 819 2340
Size: 327 492 1521
Fragments arranged by size:
1521 492 327
BanI G'GyrC_C
Cuts at: 0 195 1966 2340
Size: 195 1771 374
Fragments arranged by size:
1771 374 195
BanII G_rGCy'C
Cuts at: 0 126 235 1636 1729 2272 2340
Size: 126 109 1401 93 543 68
Fragments arranged by size:
1401 543 126 109 93 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 307 485 1919 2340
Size: 307 178 1434 421
Fragments arranged by size:
1434 421 307 178
BciVI GTATCCnnnnn_n'
Cuts at: 0 865 2340
Size: 865 1475
BclI T'GATC_A
Cuts at: 0 1503 2340
Size: 1503 837
Bme1580I G_kGCm'C
Cuts at: 0 79 1729 1971 2272 2340
Size: 79 1650 242 301 68
Fragments arranged by size:
1650 301 242 79 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 314 946 1230 2275 2340
Size: 314 632 284 1045 65
Fragments arranged by size:
1045 632 314 284 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 422 2340
Size: 219 203 1918
Fragments arranged by size:
1918 219 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1037 1150 1462 2340
Size: 191 846 113 312 878
Fragments arranged by size:
878 846 312 191 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 1880 2340
Size: 1880 460
BsaBI GATnn'nnATC
Cuts at: 0 449 2340
Size: 449 1891
BsaWI w'CCGG_w
Cuts at: 0 875 2340
Size: 875 1465
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 733 1403 2340
Size: 733 670 937
Fragments arranged by size:
937 733 670
BseYI C'CCAG_C
Cuts at: 0 698 2057 2340
Size: 698 1359 283
Fragments arranged by size:
1359 698 283
BsiHKAI G_wGCw'C
Cuts at: 0 235 432 627 2128 2340
Size: 235 197 195 1501 212
Fragments arranged by size:
1501 235 212 197 195
BsmI GAATG_Cn'
Cuts at: 0 933 2340
Size: 933 1407
Bsp1286I G_dGCh'C
Cuts at: 0 79 126 235 432 627 1636 1729 1971
Size: 79 47 109 197 195 1009 93 242
Cuts at: 1971 2128 2272 2340
Size: 157 144 68
Fragments arranged by size:
1009 242 197 195 157 144 109 93
79 68 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 261 1389 2340
Size: 261 1128 951
Fragments arranged by size:
1128 951 261
BsrFI r'CCGG_y
Cuts at: 0 738 973 2340
Size: 738 235 1367
Fragments arranged by size:
1367 738 235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 669 2340
Size: 669 1671
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 955 959 1280 2340
Size: 192 763 4 321 1060
Fragments arranged by size:
1060 763 321 192 4
BstYI r'GATC_y
Cuts at: 0 327 495 819 2340
Size: 327 168 324 1521
Fragments arranged by size:
1521 327 324 168
Bsu36I CC'TnA_GG
Cuts at: 0 607 2340
Size: 607 1733
BtsI GCAGTG_nn'
Cuts at: 0 740 2340
Size: 740 1600
DraI TTT'AAA
Cuts at: 0 2190 2340
Size: 2190 150
EaeI y'GGCC_r
Cuts at: 0 975 1698 2340
Size: 975 723 642
Fragments arranged by size:
975 723 642
EarI CTCTTCn'nnn_
Cuts at: 0 557 710 1872 1916 2340
Size: 557 153 1162 44 424
Fragments arranged by size:
1162 557 424 153 44
EcoICRI GAG'CTC
Cuts at: 0 233 2340
Size: 233 2107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 334 422 1603 1640 2340
Size: 219 115 88 1181 37 700
Fragments arranged by size:
1181 700 219 115 88 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 677 715 995 2340
Size: 677 38 280 1345
Fragments arranged by size:
1345 677 280 38
EcoO109I rG'GnC_Cy
Cuts at: 0 1726 2269 2340
Size: 1726 543 71
Fragments arranged by size:
1726 543 71
EcoRI G'AATT_C
Cuts at: 0 1015 2322 2340
Size: 1015 1307 18
Fragments arranged by size:
1307 1015 18
EcoRV GAT'ATC
Cuts at: 0 945 2340
Size: 945 1395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 906 1886 2340
Size: 906 980 454
Fragments arranged by size:
980 906 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 748 780 817 849 2122 2154 2340
Size: 748 32 37 32 1273 32 186
Fragments arranged by size:
1273 748 186 37 32 32 32
HindIII A'AGCT_T
Cuts at: 0 883 1126 1438 1652 2340
Size: 883 243 312 214 688
Fragments arranged by size:
883 688 312 243 214
KpnI G_GTAC'C
Cuts at: 0 199 2340
Size: 199 2141
MfeI C'AATT_G
Cuts at: 0 1020 1539 2340
Size: 1020 519 801
Fragments arranged by size:
1020 801 519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 2107 2340
Size: 490 1617 233
Fragments arranged by size:
1617 490 233
MscI TGG'CCA
Cuts at: 0 1700 2340
Size: 1700 640
MslI CAynn'nnrTG
Cuts at: 0 190 276 893 1526 1898 2299 2340
Size: 190 86 617 633 372 401 41
Fragments arranged by size:
633 617 401 372 190 86 41
MspA1I CmG'CkG
Cuts at: 0 2011 2340
Size: 2011 329
NaeI GCC'GGC
Cuts at: 0 740 2340
Size: 740 1600
NgoMIV G'CCGG_C
Cuts at: 0 738 2340
Size: 738 1602
NspI r_CATG'y
Cuts at: 0 1046 1897 2340
Size: 1046 851 443
Fragments arranged by size:
1046 851 443
PciI A'CATG_T
Cuts at: 0 1042 2340
Size: 1042 1298
PflMI CCAn_nnn'nTGG
Cuts at: 0 188 2340
Size: 188 2152
PspOMI G'GGCC_C
Cuts at: 0 1725 2268 2340
Size: 1725 543 72
Fragments arranged by size:
1725 543 72
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1293 1325 2340
Size: 1293 32 1015
Fragments arranged by size:
1293 1015 32
PstI C_TGCA'G
Cuts at: 0 2316 2340
Size: 2316 24
PvuII CAG'CTG
Cuts at: 0 2011 2340
Size: 2011 329
SacI G_AGCT'C
Cuts at: 0 235 2340
Size: 235 2105
SapI GCTCTTCn'nnn_
Cuts at: 0 1872 2340
Size: 1872 468
SexAI A'CCwGG_T
Cuts at: 0 1317 2340
Size: 1317 1023
SfcI C'TryA_G
Cuts at: 0 1586 2312 2340
Size: 1586 726 28
Fragments arranged by size:
1586 726 28
SmaI CCC'GGG
Cuts at: 0 1682 1972 2340
Size: 1682 290 368
Fragments arranged by size:
1682 368 290
SmlI C'TyrA_G
Cuts at: 0 206 1036 1052 1129 1441 1797 2340
Size: 206 830 16 77 312 356 543
Fragments arranged by size:
830 543 356 312 206 77 16
SphI G_CATG'C
Cuts at: 0 1897 2340
Size: 1897 443
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 912 947 965 1994 2153 2340
Size: 912 35 18 1029 159 187
Fragments arranged by size:
1029 912 187 159 35 18
TatI w'GTAC_w
Cuts at: 0 654 839 2340
Size: 654 185 1501
Fragments arranged by size:
1501 654 185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 257 2340
Size: 189 68 2083
Fragments arranged by size:
2083 189 68
XmaI C'CCGG_G
Cuts at: 0 1680 1970 2340
Size: 1680 290 370
Fragments arranged by size:
1680 370 290
XmnI GAAnn'nnTTC
Cuts at: 0 347 748 2216 2340
Size: 347 401 1468 124
Fragments arranged by size:
1468 401 347 124
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AloI
ApaI ApoI AseI AvaI BamHI BanI BanII BbsI
BciVI BclI Bme1580I BmrI BpmI BpuEI BsaI BsaBI
BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BstAPI BstXI BstYI Bsu36I BtsI DraI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PciI PflMI PspOMI PsrI PstI PvuII
SacI SapI SexAI SfcI SmaI SmlI SphI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AleI AlfI AlwNI ApaLI
AscI AsiSI AvrII BaeI BbeI BbvCI BcgI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI
BsaHI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI
FseI FspI FspAI HaeII HincII HpaI KasI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI StyI SwaI TstI Tth111I XbaI XhoI ZraI