Restriction map for
pF1KSDA1792
(Linear) (Six-base) MAPSORT of: KIAA1792.seq Check: 479 from: 1 to: 2292
KIAA1792.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1421 1429 2292
Size: 1421 8 863
Fragments arranged by size:
1421 863 8
Acc65I G'GTAC_C
Cuts at: 0 955 2292
Size: 955 1337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1594 1813 2292
Size: 1594 219 479
Fragments arranged by size:
1594 479 219
AflII C'TTAA_G
Cuts at: 0 1531 2292
Size: 1531 761
AleI CACnn'nnGTG
Cuts at: 0 1099 2292
Size: 1099 1193
AlwNI CAG_nnn'CTG
Cuts at: 0 414 1050 1243 1948 2292
Size: 414 636 193 705 344
Fragments arranged by size:
705 636 414 344 193
ApaI G_GGCC'C
Cuts at: 0 1718 2292
Size: 1718 574
ApoI r'AATT_y
Cuts at: 0 235 1000 1151 1345 1390 1659 1672 2292
Size: 235 765 151 194 45 269 13 620
Fragments arranged by size:
765 620 269 235 194 151 45 13
AvrII C'CTAG_G
Cuts at: 0 775 2292
Size: 775 1517
BanI G'GyrC_C
Cuts at: 0 955 2292
Size: 955 1337
BanII G_rGCy'C
Cuts at: 0 283 514 1718 2292
Size: 283 231 1204 574
Fragments arranged by size:
1204 574 283 231
BbvCI CC'TCA_GC
Cuts at: 0 1145 2292
Size: 1145 1147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 29 63 2158 2192 2292
Size: 29 34 2095 34 100
Fragments arranged by size:
2095 100 34 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 891 2292
Size: 891 1401
BclI T'GATC_A
Cuts at: 0 617 935 2292
Size: 617 318 1357
Fragments arranged by size:
1357 617 318
BfrBI ATG'CAT
Cuts at: 0 394 2292
Size: 394 1898
BglI GCCn_nnn'nGGC
Cuts at: 0 1430 1825 2292
Size: 1430 395 467
Fragments arranged by size:
1430 467 395
BglII A'GATC_T
Cuts at: 0 612 2292
Size: 612 1680
BlpI GC'TnA_GC
Cuts at: 0 508 2292
Size: 508 1784
Bme1580I G_kGCm'C
Cuts at: 0 222 1718 2056 2292
Size: 222 1496 338 236
Fragments arranged by size:
1496 338 236 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 1713 2220 2292
Size: 1713 507 72
Fragments arranged by size:
1713 507 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 457 771 1338 1717 2115 2292
Size: 113 344 314 567 379 398 177
Fragments arranged by size:
567 398 379 344 314 177 113
Bpu10I CC'TnA_GC
Cuts at: 0 1145 1505 2292
Size: 1145 360 787
Fragments arranged by size:
1145 787 360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1873 2292
Size: 464 1409 419
Fragments arranged by size:
1409 464 419
BsaI GGTCTCn'nnnn_
Cuts at: 0 679 1693 2292
Size: 679 1014 599
Fragments arranged by size:
1014 679 599
BsaAI yAC'GTr
Cuts at: 0 1101 2292
Size: 1101 1191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 961 1133 2292
Size: 961 172 1159
Fragments arranged by size:
1159 961 172
BseYI C'CCAG_C
Cuts at: 0 276 543 1428 1707 2292
Size: 276 267 885 279 585
Fragments arranged by size:
885 585 279 276 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 2134 2292
Size: 89 2045 158
Fragments arranged by size:
2045 158 89
BsiHKAI G_wGCw'C
Cuts at: 0 514 2292
Size: 514 1778
BsmI GAATG_Cn'
Cuts at: 0 1239 2292
Size: 1239 1053
Bsp1286I G_dGCh'C
Cuts at: 0 222 283 514 1718 2056 2292
Size: 222 61 231 1204 338 236
Fragments arranged by size:
1204 338 236 231 222 61
BspHI T'CATG_A
Cuts at: 0 1118 2265 2292
Size: 1118 1147 27
Fragments arranged by size:
1147 1118 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1421 1429 2292
Size: 1421 8 863
Fragments arranged by size:
1421 863 8
BsrDI GCAATG_nn'
Cuts at: 0 268 2016 2292
Size: 268 1748 276
Fragments arranged by size:
1748 276 268
BsrFI r'CCGG_y
Cuts at: 0 1824 2292
Size: 1824 468
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1243 2292
Size: 1243 1049
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 1968 2040 2292
Size: 966 1002 72 252
Fragments arranged by size:
1002 966 252 72
BstYI r'GATC_y
Cuts at: 0 612 822 2136 2292
Size: 612 210 1314 156
Fragments arranged by size:
1314 612 210 156
BtgI C'CryG_G
Cuts at: 0 1961 2033 2292
Size: 1961 72 259
Fragments arranged by size:
1961 259 72
BtsI GCAGTG_nn'
Cuts at: 0 222 1244 2292
Size: 222 1022 1048
Fragments arranged by size:
1048 1022 222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 282 317 2292
Size: 282 35 1975
Fragments arranged by size:
1975 282 35
DraI TTT'AAA
Cuts at: 0 785 978 2292
Size: 785 193 1314
Fragments arranged by size:
1314 785 193
DrdI GACnn_nn'nnGTC
Cuts at: 0 1771 2292
Size: 1771 521
EarI CTCTTCn'nnn_
Cuts at: 0 195 302 2155 2292
Size: 195 107 1853 137
Fragments arranged by size:
1853 195 137 107
EcoICRI GAG'CTC
Cuts at: 0 512 2292
Size: 512 1780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 457 771 1338 1594 1717 1813 2115
Size: 113 344 314 567 256 123 96 302
Cuts at: 2115 2292
Size: 177
Fragments arranged by size:
567 344 314 302 256 177 123 113
96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1531 2292
Size: 1531 761
EcoO109I rG'GnC_Cy
Cuts at: 0 123 888 1714 1715 2292
Size: 123 765 826 1 577
Fragments arranged by size:
826 765 577 123 1
EcoRI G'AATT_C
Cuts at: 0 235 1151 1390 1659 1672 2292
Size: 235 916 239 269 13 620
Fragments arranged by size:
916 620 269 239 235 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1128 1160 1731 1763 2292
Size: 1128 32 571 32 529
Fragments arranged by size:
1128 571 529 32 32
HincII GTy'rAC
Cuts at: 0 1788 2292
Size: 1788 504
HindIII A'AGCT_T
Cuts at: 0 632 2292
Size: 632 1660
HpaI GTT'AAC
Cuts at: 0 1788 2292
Size: 1788 504
KpnI G_GTAC'C
Cuts at: 0 959 2292
Size: 959 1333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1949 2292
Size: 1949 343
MslI CAynn'nnrTG
Cuts at: 0 1099 2038 2292
Size: 1099 939 254
Fragments arranged by size:
1099 939 254
MspA1I CmG'CkG
Cuts at: 0 276 576 1984 2172 2292
Size: 276 300 1408 188 120
Fragments arranged by size:
1408 300 276 188 120
NaeI GCC'GGC
Cuts at: 0 1826 2292
Size: 1826 466
NcoI C'CATG_G
Cuts at: 0 1961 2033 2292
Size: 1961 72 259
Fragments arranged by size:
1961 259 72
NdeI CA'TA_TG
Cuts at: 0 175 1736 2292
Size: 175 1561 556
Fragments arranged by size:
1561 556 175
NgoMIV G'CCGG_C
Cuts at: 0 1824 2292
Size: 1824 468
NsiI A_TGCA'T
Cuts at: 0 396 2292
Size: 396 1896
NspI r_CATG'y
Cuts at: 0 345 1861 2070 2292
Size: 345 1516 209 222
Fragments arranged by size:
1516 345 222 209
PasI CC'CwG_GG
Cuts at: 0 287 1161 2292
Size: 287 874 1131
Fragments arranged by size:
1131 874 287
PmlI CAC'GTG
Cuts at: 0 1101 2292
Size: 1101 1191
PpuMI rG'GwC_Cy
Cuts at: 0 123 888 2292
Size: 123 765 1404
Fragments arranged by size:
1404 765 123
PsiI TTA'TAA
Cuts at: 0 1325 2292
Size: 1325 967
PspOMI G'GGCC_C
Cuts at: 0 1714 2292
Size: 1714 578
PstI C_TGCA'G
Cuts at: 0 525 2292
Size: 525 1767
PvuII CAG'CTG
Cuts at: 0 276 576 1984 2172 2292
Size: 276 300 1408 188 120
Fragments arranged by size:
1408 300 276 188 120
SacI G_AGCT'C
Cuts at: 0 514 2292
Size: 514 1778
ScaI AGT'ACT
Cuts at: 0 987 1450 1602 2292
Size: 987 463 152 690
Fragments arranged by size:
987 690 463 152
SfcI C'TryA_G
Cuts at: 0 355 521 2292
Size: 355 166 1771
Fragments arranged by size:
1771 355 166
SmlI C'TyrA_G
Cuts at: 0 13 479 1531 1852 2292
Size: 13 466 1052 321 440
Fragments arranged by size:
1052 466 440 321 13
SphI G_CATG'C
Cuts at: 0 2070 2292
Size: 2070 222
SspI AAT'ATT
Cuts at: 0 1074 2292
Size: 1074 1218
StyI C'CwwG_G
Cuts at: 0 775 1961 2033 2142 2292
Size: 775 1186 72 109 150
Fragments arranged by size:
1186 775 150 109 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 239 300 1347 2292
Size: 239 61 1047 945
Fragments arranged by size:
1047 945 239 61
TatI w'GTAC_w
Cuts at: 0 265 985 1448 1600 2292
Size: 265 720 463 152 692
Fragments arranged by size:
720 692 463 265 152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1425 2122 2292
Size: 1425 697 170
Fragments arranged by size:
1425 697 170
XmnI GAAnn'nnTTC
Cuts at: 0 1155 2275 2292
Size: 1155 1120 17
Fragments arranged by size:
1155 1120 17
Enzymes that do cut:
AarI Acc65I AcuI AflII AleI AlwNI ApaI ApoI
AvrII BanI BanII BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI
BtsI CspCI DraI DrdI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII HindIII HpaI KpnI MmeI
MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI
PasI PmlI PpuMI PsiI PspOMI PstI PvuII SacI
ScaI SfcI SmlI SphI SspI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvaI BaeI BamHI
BbeI BbsI BmgBI BmtI BplI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraIII
EaeI EagI EciI EcoRV FalI FseI FspI FspAI
HaeII KasI MfeI MluI MscI NarI NheI NotI
NruI PacI PciI PflMI PfoI PmeI PpiI PshAI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI