Restriction map for
pF1KA1782
(Linear) (Six-base) MAPSORT of: KIAA1782.seq Check: 1966 from: 1 to: 1614
KIAA1782.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1111 1614
Size: 1111 503
AatII G_ACGT'C
Cuts at: 0 347 1614
Size: 347 1267
AccI GT'mk_AC
Cuts at: 0 1064 1092 1614
Size: 1064 28 522
Fragments arranged by size:
1064 522 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 858 1503 1614
Size: 716 142 645 111
Fragments arranged by size:
716 645 142 111
AgeI A'CCGG_T
Cuts at: 0 1565 1614
Size: 1565 49
AleI CACnn'nnGTG
Cuts at: 0 404 1312 1614
Size: 404 908 302
Fragments arranged by size:
908 404 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 334 1349 1614
Size: 334 1015 265
Fragments arranged by size:
1015 334 265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 1614
Size: 52 32 1530
Fragments arranged by size:
1530 52 32
ApaI G_GGCC'C
Cuts at: 0 81 92 1614
Size: 81 11 1522
Fragments arranged by size:
1522 81 11
ApaLI G'TGCA_C
Cuts at: 0 385 1614
Size: 385 1229
ApoI r'AATT_y
Cuts at: 0 1573 1614
Size: 1573 41
AvaI C'yCGr_G
Cuts at: 0 73 906 1112 1172 1614
Size: 73 833 206 60 442
Fragments arranged by size:
833 442 206 73 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1449 1482 1614
Size: 1449 33 132
Fragments arranged by size:
1449 132 33
BamHI G'GATC_C
Cuts at: 0 1108 1614
Size: 1108 506
BanI G'GyrC_C
Cuts at: 0 436 665 750 1381 1614
Size: 436 229 85 631 233
Fragments arranged by size:
631 436 233 229 85
BanII G_rGCy'C
Cuts at: 0 81 92 263 1614
Size: 81 11 171 1351
Fragments arranged by size:
1351 171 81 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 1614
Size: 17 1597
BciVI GTATCCnnnnn_n'
Cuts at: 0 1281 1477 1614
Size: 1281 196 137
Fragments arranged by size:
1281 196 137
Bme1580I G_kGCm'C
Cuts at: 0 81 92 389 1384 1614
Size: 81 11 297 995 230
Fragments arranged by size:
995 297 230 81 11
BmgBI CAC'GTC
Cuts at: 0 1485 1614
Size: 1485 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 76 1614
Size: 76 1538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 1174 1614
Size: 315 859 440
Fragments arranged by size:
859 440 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 667 763 1117 1614
Size: 79 588 96 354 497
Fragments arranged by size:
588 497 354 96 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1614
Size: 319 1295
BsaI GGTCTCn'nnnn_
Cuts at: 0 1517 1614
Size: 1517 97
BsaAI yAC'GTr
Cuts at: 0 736 1614
Size: 736 878
BsaHI Gr'CG_yC
Cuts at: 0 344 1614
Size: 344 1270
BsaWI w'CCGG_w
Cuts at: 0 1565 1614
Size: 1565 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 559 589 1614
Size: 52 30 477 30 1025
Fragments arranged by size:
1025 477 52 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 567 664 1150 1614
Size: 193 374 97 486 464
Fragments arranged by size:
486 464 374 193 97
BseYI C'CCAG_C
Cuts at: 0 195 1204 1295 1614
Size: 195 1009 91 319
Fragments arranged by size:
1009 319 195 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 751 1010 1614
Size: 466 285 259 604
Fragments arranged by size:
604 466 285 259
BsiEI CG_ry'CG
Cuts at: 0 801 1614
Size: 801 813
BsiHKAI G_wGCw'C
Cuts at: 0 103 380 389 656 1460 1614
Size: 103 277 9 267 804 154
Fragments arranged by size:
804 277 267 154 103 9
Bsp1286I G_dGCh'C
Cuts at: 0 81 92 103 263 380 389 656 1384
Size: 81 11 11 160 117 9 267 728
Cuts at: 1384 1460 1614
Size: 76 154
Fragments arranged by size:
728 267 160 154 117 81 76 11
11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 1111 1614
Size: 470 641 503
Fragments arranged by size:
641 503 470
BsrBI CCG'CTC
Cuts at: 0 26 134 210 663 783 1614
Size: 26 108 76 453 120 831
Fragments arranged by size:
831 453 120 108 76 26
BsrDI GCAATG_nn'
Cuts at: 0 24 420 1215 1614
Size: 24 396 795 399
Fragments arranged by size:
795 399 396 24
BsrFI r'CCGG_y
Cuts at: 0 533 1166 1322 1565 1614
Size: 533 633 156 243 49
Fragments arranged by size:
633 533 243 156 49
BsrGI T'GTAC_A
Cuts at: 0 167 1614
Size: 167 1447
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1214 1614
Size: 1214 400
BstEII G'GTnAC_C
Cuts at: 0 553 1614
Size: 553 1061
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 1314 1317 1614
Size: 159 1155 3 297
Fragments arranged by size:
1155 297 159 3
BstYI r'GATC_y
Cuts at: 0 1108 1614
Size: 1108 506
Bsu36I CC'TnA_GG
Cuts at: 0 1133 1280 1614
Size: 1133 147 334
Fragments arranged by size:
1133 334 147
BtgI C'CryG_G
Cuts at: 0 1512 1614
Size: 1512 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 188 1614
Size: 188 1426
BtsI GCAGTG_nn'
Cuts at: 0 1458 1614
Size: 1458 156
ClaI AT'CG_AT
Cuts at: 0 797 1614
Size: 797 817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 414 449 1527 1562 1614
Size: 414 35 1078 35 52
Fragments arranged by size:
1078 414 52 35 35
DraI TTT'AAA
Cuts at: 0 504 1614
Size: 504 1110
DraIII CAC_nnn'GTG
Cuts at: 0 142 1614
Size: 142 1472
EaeI y'GGCC_r
Cuts at: 0 21 618 714 784 806 1614
Size: 21 597 96 70 22 808
Fragments arranged by size:
808 597 96 70 22 21
EarI CTCTTCn'nnn_
Cuts at: 0 126 1477 1584 1614
Size: 126 1351 107 30
Fragments arranged by size:
1351 126 107 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1000 1614
Size: 1000 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 667 716 763 858 1117 1503 1614
Size: 79 588 49 47 95 259 386 111
Fragments arranged by size:
588 386 259 111 95 79 49 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1279 1614
Size: 220 1059 335
Fragments arranged by size:
1059 335 220
EcoO109I rG'GnC_Cy
Cuts at: 0 77 78 88 89 413 1129 1137 1153
Size: 77 1 10 1 324 716 8 16
Cuts at: 1153 1177 1285 1350 1390 1614
Size: 24 108 65 40 224
Fragments arranged by size:
716 324 224 108 77 65 40 24
16 10 8 1 1
EcoRI G'AATT_C
Cuts at: 0 1573 1614
Size: 1573 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 238 270 889 921 1614
Size: 166 32 40 32 619 32 693
Fragments arranged by size:
693 619 166 40 32 32 32
HincII GTy'rAC
Cuts at: 0 1093 1614
Size: 1093 521
HindIII A'AGCT_T
Cuts at: 0 704 1614
Size: 704 910
MfeI C'AATT_G
Cuts at: 0 1026 1614
Size: 1026 588
MscI TGG'CCA
Cuts at: 0 716 808 1614
Size: 716 92 806
Fragments arranged by size:
806 716 92
MslI CAynn'nnrTG
Cuts at: 0 157 404 1072 1312 1315 1614
Size: 157 247 668 240 3 299
Fragments arranged by size:
668 299 247 240 157 3
NaeI GCC'GGC
Cuts at: 0 535 1324 1614
Size: 535 789 290
Fragments arranged by size:
789 535 290
NcoI C'CATG_G
Cuts at: 0 1512 1614
Size: 1512 102
NgoMIV G'CCGG_C
Cuts at: 0 533 1322 1614
Size: 533 789 292
Fragments arranged by size:
789 533 292
PasI CC'CwG_GG
Cuts at: 0 264 304 1192 1614
Size: 264 40 888 422
Fragments arranged by size:
888 422 264 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 708 1614
Size: 708 906
PfoI T'CCnGG_A
Cuts at: 0 215 1104 1475 1614
Size: 215 889 371 139
Fragments arranged by size:
889 371 215 139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 1614
Size: 52 32 1530
Fragments arranged by size:
1530 52 32
PpuMI rG'GwC_Cy
Cuts at: 0 1129 1137 1153 1285 1350 1614
Size: 1129 8 16 132 65 264
Fragments arranged by size:
1129 264 132 65 16 8
PspOMI G'GGCC_C
Cuts at: 0 77 88 1614
Size: 77 11 1526
Fragments arranged by size:
1526 77 11
PvuI CG_AT'CG
Cuts at: 0 801 1614
Size: 801 813
SalI G'TCGA_C
Cuts at: 0 1091 1614
Size: 1091 523
SanDI GG'GwC_CC
Cuts at: 0 1285 1614
Size: 1285 329
SexAI A'CCwGG_T
Cuts at: 0 720 1614
Size: 720 894
SfcI C'TryA_G
Cuts at: 0 679 1614
Size: 679 935
SgrAI Cr'CCGG_yG
Cuts at: 0 533 1614
Size: 533 1081
SmlI C'TyrA_G
Cuts at: 0 334 1614
Size: 334 1280
StuI AGG'CCT
Cuts at: 0 1444 1614
Size: 1444 170
StyI C'CwwG_G
Cuts at: 0 1397 1512 1614
Size: 1397 115 102
Fragments arranged by size:
1397 115 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 976 1035 1084 1614
Size: 463 513 59 49 530
Fragments arranged by size:
530 513 463 59 49
TatI w'GTAC_w
Cuts at: 0 167 1614
Size: 167 1447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 359 391 559 591 1614
Size: 359 32 168 32 1023
Fragments arranged by size:
1023 359 168 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1483 1614
Size: 1483 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 709 1314 1614
Size: 378 331 605 300
Fragments arranged by size:
605 378 331 300
XmnI GAAnn'nnTTC
Cuts at: 0 67 1406 1444 1614
Size: 67 1339 38 170
Fragments arranged by size:
1339 170 67 38
ZraI GAC'GTC
Cuts at: 0 345 1614
Size: 345 1269
Enzymes that do cut:
AarI AatII AccI AcuI AgeI AleI AlfI AloI
ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbsI BciVI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI Hin4I HincII HindIII MfeI MscI
MslI NaeI NcoI NgoMIV PasI PflMI PfoI PpiI
PpuMI PspOMI PvuI SalI SanDI SexAI SfcI SgrAI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AhdI AlwNI AscI
AseI AsiSI AvrII BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmtI Bpu10I BsaBI BsiWI BsmI
BsmBI BspEI BspHI BssHII BssSI BstBI BstZ17I DrdI
EagI EcoICRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI MmeI MspA1I NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuII RsrII
SacI SacII SapI SbfI ScaI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmaI