Restriction map for
pF1KSDA1754
(Linear) (Six-base) MAPSORT of: KIAA1754.seq Check: 9322 from: 1 to: 1641
KIAA1754.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 201 591 1641
Size: 201 390 1050
Fragments arranged by size:
1050 390 201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1318 1641
Size: 1021 297 323
Fragments arranged by size:
1021 323 297
AfeI AGC'GCT
Cuts at: 0 442 1586 1641
Size: 442 1144 55
Fragments arranged by size:
1144 442 55
AflIII A'CryG_T
Cuts at: 0 246 1608 1641
Size: 246 1362 33
Fragments arranged by size:
1362 246 33
AgeI A'CCGG_T
Cuts at: 0 1267 1641
Size: 1267 374
AleI CACnn'nnGTG
Cuts at: 0 339 618 1641
Size: 339 279 1023
Fragments arranged by size:
1023 339 279
AlwNI CAG_nnn'CTG
Cuts at: 0 932 1641
Size: 932 709
ApaI G_GGCC'C
Cuts at: 0 332 1641
Size: 332 1309
AvaI C'yCGr_G
Cuts at: 0 91 479 649 784 990 1198 1641
Size: 91 388 170 135 206 208 443
Fragments arranged by size:
443 388 208 206 170 135 91
BanI G'GyrC_C
Cuts at: 0 382 828 1114 1204 1449 1641
Size: 382 446 286 90 245 192
Fragments arranged by size:
446 382 286 245 192 90
BanII G_rGCy'C
Cuts at: 0 14 332 656 677 1106 1283 1496 1641
Size: 14 318 324 21 429 177 213 145
Fragments arranged by size:
429 324 318 213 177 145 21 14
BbeI G_GCGC'C
Cuts at: 0 386 832 1208 1641
Size: 386 446 376 433
Fragments arranged by size:
446 433 386 376
BbsI GAAGACnn'nnnn_
Cuts at: 0 797 1306 1641
Size: 797 509 335
Fragments arranged by size:
797 509 335
BbvCI CC'TCA_GC
Cuts at: 0 1216 1641
Size: 1216 425
BlpI GC'TnA_GC
Cuts at: 0 1282 1641
Size: 1282 359
Bme1580I G_kGCm'C
Cuts at: 0 332 638 701 1117 1641
Size: 332 306 63 416 524
Fragments arranged by size:
524 416 332 306 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 1641
Size: 348 1293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 184 226 285 1110 1393 1414 1641
Size: 169 15 42 59 825 283 21 227
Fragments arranged by size:
825 283 227 169 59 42 21 15
Bpu10I CC'TnA_GC
Cuts at: 0 1216 1483 1641
Size: 1216 267 158
Fragments arranged by size:
1216 267 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1555 1641
Size: 1555 86
BsaHI Gr'CG_yC
Cuts at: 0 383 829 1205 1641
Size: 383 446 376 436
Fragments arranged by size:
446 436 383 376
BsaWI w'CCGG_w
Cuts at: 0 1267 1641
Size: 1267 374
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1069 1099 1301 1331 1641
Size: 1069 30 202 30 310
Fragments arranged by size:
1069 310 202 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 118 181 184 235 370 540 1315
Size: 115 3 63 3 51 135 170 775
Cuts at: 1315 1641
Size: 326
Fragments arranged by size:
775 326 170 135 115 63 51 3
3
BseYI C'CCAG_C
Cuts at: 0 375 1119 1273 1581 1641
Size: 375 744 154 308 60
Fragments arranged by size:
744 375 308 154 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1297 1357 1641
Size: 793 504 60 284
Fragments arranged by size:
793 504 284 60
BsiEI CG_ry'CG
Cuts at: 0 191 464 690 1641
Size: 191 273 226 951
Fragments arranged by size:
951 273 226 191
BsiHKAI G_wGCw'C
Cuts at: 0 271 677 685 1484 1641
Size: 271 406 8 799 157
Fragments arranged by size:
799 406 271 157 8
BsmI GAATG_Cn'
Cuts at: 0 1581 1641
Size: 1581 60
Bsp1286I G_dGCh'C
Cuts at: 0 14 271 332 638 656 677 685 701
Size: 14 257 61 306 18 21 8 16
Cuts at: 701 1106 1117 1283 1484 1496 1641
Size: 405 11 166 201 12 145
Fragments arranged by size:
405 306 257 201 166 145 61 21
18 16 14 12 11 8
BspHI T'CATG_A
Cuts at: 0 899 1641
Size: 899 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 591 863 885 1641
Size: 201 390 272 22 756
Fragments arranged by size:
756 390 272 201 22
BsrBI CCG'CTC
Cuts at: 0 692 1641
Size: 692 949
BsrFI r'CCGG_y
Cuts at: 0 1267 1641
Size: 1267 374
BssSI C'ACGA_G
Cuts at: 0 1375 1641
Size: 1375 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 275 1380 1641
Size: 275 1105 261
Fragments arranged by size:
1105 275 261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 677 1641
Size: 677 964
Bsu36I CC'TnA_GG
Cuts at: 0 523 1174 1454 1472 1641
Size: 523 651 280 18 169
Fragments arranged by size:
651 523 280 169 18
BtgI C'CryG_G
Cuts at: 0 5 70 458 1460 1641
Size: 5 65 388 1002 181
Fragments arranged by size:
1002 388 181 65 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 1641
Size: 927 714
BtsI GCAGTG_nn'
Cuts at: 0 613 1192 1641
Size: 613 579 449
Fragments arranged by size:
613 579 449
EaeI y'GGCC_r
Cuts at: 0 2 41 188 426 461 687 720 975
Size: 2 39 147 238 35 226 33 255
Cuts at: 975 1641
Size: 666
Fragments arranged by size:
666 255 238 226 147 39 35 33
2
EagI C'GGCC_G
Cuts at: 0 188 461 687 1641
Size: 188 273 226 954
Fragments arranged by size:
954 273 226 188
EarI CTCTTCn'nnn_
Cuts at: 0 19 292 1393 1510 1641
Size: 19 273 1101 117 131
Fragments arranged by size:
1101 273 131 117 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 1641
Size: 232 1409
EcoICRI GAG'CTC
Cuts at: 0 675 1641
Size: 675 966
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 184 226 285 1021 1110 1318 1393
Size: 169 15 42 59 736 89 208 75
Cuts at: 1393 1414 1641
Size: 21 227
Fragments arranged by size:
736 227 208 169 89 75 59 42
21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 1330 1641
Size: 166 1164 311
Fragments arranged by size:
1164 311 166
EcoO109I rG'GnC_Cy
Cuts at: 0 258 328 329 396 995 1641
Size: 258 70 1 67 599 646
Fragments arranged by size:
646 599 258 70 67 1
HaeII r_GCGC'y
Cuts at: 0 386 444 832 1208 1588 1641
Size: 386 58 388 376 380 53
Fragments arranged by size:
388 386 380 376 58 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1157 1189 1641
Size: 1157 32 452
Fragments arranged by size:
1157 452 32
KasI G'GCGC_C
Cuts at: 0 382 828 1204 1641
Size: 382 446 376 437
Fragments arranged by size:
446 437 382 376
MluI A'CGCG_T
Cuts at: 0 246 1641
Size: 246 1395
MscI TGG'CCA
Cuts at: 0 4 428 977 1641
Size: 4 424 549 664
Fragments arranged by size:
664 549 424 4
MslI CAynn'nnrTG
Cuts at: 0 339 434 550 556 574 618 1641
Size: 339 95 116 6 18 44 1023
Fragments arranged by size:
1023 339 116 95 44 18 6
MspA1I CmG'CkG
Cuts at: 0 60 72 147 981 1220 1277 1359 1641
Size: 60 12 75 834 239 57 82 282
Fragments arranged by size:
834 282 239 82 75 60 57 12
NarI GG'CG_CC
Cuts at: 0 383 829 1205 1641
Size: 383 446 376 436
Fragments arranged by size:
446 436 383 376
NcoI C'CATG_G
Cuts at: 0 5 1460 1641
Size: 5 1455 181
Fragments arranged by size:
1455 181 5
NspI r_CATG'y
Cuts at: 0 588 798 1612 1641
Size: 588 210 814 29
Fragments arranged by size:
814 588 210 29
PasI CC'CwG_GG
Cuts at: 0 1097 1641
Size: 1097 544
PciI A'CATG_T
Cuts at: 0 1608 1641
Size: 1608 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 932 1641
Size: 932 709
PfoI T'CCnGG_A
Cuts at: 0 488 1391 1641
Size: 488 903 250
Fragments arranged by size:
903 488 250
PpuMI rG'GwC_Cy
Cuts at: 0 258 995 1641
Size: 258 737 646
Fragments arranged by size:
737 646 258
PshAI GACnn'nnGTC
Cuts at: 0 1367 1641
Size: 1367 274
PspOMI G'GGCC_C
Cuts at: 0 328 1641
Size: 328 1313
PstI C_TGCA'G
Cuts at: 0 146 1229 1526 1641
Size: 146 1083 297 115
Fragments arranged by size:
1083 297 146 115
PvuII CAG'CTG
Cuts at: 0 147 981 1220 1359 1641
Size: 147 834 239 139 282
Fragments arranged by size:
834 282 239 147 139
SacI G_AGCT'C
Cuts at: 0 677 1641
Size: 677 964
SacII CC_GC'GG
Cuts at: 0 73 1641
Size: 73 1568
SanDI GG'GwC_CC
Cuts at: 0 995 1641
Size: 995 646
SapI GCTCTTCn'nnn_
Cuts at: 0 19 1393 1641
Size: 19 1374 248
Fragments arranged by size:
1374 248 19
SfcI C'TryA_G
Cuts at: 0 142 1225 1522 1641
Size: 142 1083 297 119
Fragments arranged by size:
1083 297 142 119
SfoI GGC'GCC
Cuts at: 0 384 830 1206 1641
Size: 384 446 376 435
Fragments arranged by size:
446 435 384 376
SmaI CCC'GGG
Cuts at: 0 481 992 1641
Size: 481 511 649
Fragments arranged by size:
649 511 481
SmlI C'TyrA_G
Cuts at: 0 1570 1641
Size: 1570 71
StyI C'CwwG_G
Cuts at: 0 5 1460 1641
Size: 5 1455 181
Fragments arranged by size:
1455 181 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 683 1641
Size: 683 958
TatI w'GTAC_w
Cuts at: 0 1076 1641
Size: 1076 565
XmaI C'CCGG_G
Cuts at: 0 479 990 1641
Size: 479 511 651
Fragments arranged by size:
651 511 479
XmnI GAAnn'nnTTC
Cuts at: 0 907 1641
Size: 907 734
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AleI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrFI BssSI BstAPI BstXI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MluI MscI MslI MspA1I
NarI NcoI NspI PasI PciI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SanDI SapI
SfcI SfoI SmaI SmlI StyI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI
BmtI BsaI BsaAI BsaBI BsiWI BsmBI BspEI BsrDI
BsrGI BssHII BstBI BstEII BstYI BstZ17I ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI ZraI