Restriction map for
pF1KSDA1748
(Linear) (Six-base) MAPSORT of: KIAA1748.seq Check: 5108 from: 1 to: 2145
KIAA1748.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 141 331 2145
Size: 141 190 1814
Fragments arranged by size:
1814 190 141
Acc65I G'GTAC_C
Cuts at: 0 2122 2145
Size: 2122 23
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1412 2017 2145
Size: 168 1244 605 128
Fragments arranged by size:
1244 605 168 128
AflIII A'CryG_T
Cuts at: 0 103 2145
Size: 103 2042
AhdI GACnn_n'nnGTC
Cuts at: 0 1605 2145
Size: 1605 540
AleI CACnn'nnGTG
Cuts at: 0 359 2145
Size: 359 1786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 2145
Size: 316 1829
AlwNI CAG_nnn'CTG
Cuts at: 0 13 365 928 1465 2145
Size: 13 352 563 537 680
Fragments arranged by size:
680 563 537 352 13
ApoI r'AATT_y
Cuts at: 0 385 670 2145
Size: 385 285 1475
Fragments arranged by size:
1475 385 285
AvaI C'yCGr_G
Cuts at: 0 221 1671 1728 2145
Size: 221 1450 57 417
Fragments arranged by size:
1450 417 221 57
BanI G'GyrC_C
Cuts at: 0 1348 1541 1554 2122 2145
Size: 1348 193 13 568 23
Fragments arranged by size:
1348 568 193 23 13
BanII G_rGCy'C
Cuts at: 0 264 1199 1583 2145
Size: 264 935 384 562
Fragments arranged by size:
935 562 384 264
BbsI GAAGACnn'nnnn_
Cuts at: 0 784 2005 2145
Size: 784 1221 140
Fragments arranged by size:
1221 784 140
BbvCI CC'TCA_GC
Cuts at: 0 227 871 2145
Size: 227 644 1274
Fragments arranged by size:
1274 644 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1318 1352 2145
Size: 1318 34 793
Fragments arranged by size:
1318 793 34
BclI T'GATC_A
Cuts at: 0 390 2145
Size: 390 1755
BglI GCCn_nnn'nGGC
Cuts at: 0 1516 1836 2145
Size: 1516 320 309
Fragments arranged by size:
1516 320 309
BlpI GC'TnA_GC
Cuts at: 0 2043 2145
Size: 2043 102
Bme1580I G_kGCm'C
Cuts at: 0 143 1306 1351 2145
Size: 143 1163 45 794
Fragments arranged by size:
1163 794 143 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 1807 2145
Size: 1807 338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 549 1170 2145
Size: 549 621 975
Fragments arranged by size:
975 621 549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 919 1095 1266 1615 2145
Size: 725 194 176 171 349 530
Fragments arranged by size:
725 530 349 194 176 171
Bpu10I CC'TnA_GC
Cuts at: 0 227 871 947 958 1466 1840 2037 2145
Size: 227 644 76 11 508 374 197 108
Fragments arranged by size:
644 508 374 227 197 108 76 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 2145
Size: 1273 872
BsaI GGTCTCn'nnnn_
Cuts at: 0 54 756 2145
Size: 54 702 1389
Fragments arranged by size:
1389 702 54
BsaAI yAC'GTr
Cuts at: 0 104 621 2145
Size: 104 517 1524
Fragments arranged by size:
1524 517 104
BsaHI Gr'CG_yC
Cuts at: 0 1803 2145
Size: 1803 342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1652 1682 2145
Size: 1652 30 463
Fragments arranged by size:
1652 463 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 888 932 1269 1746 2108 2145
Size: 244 644 44 337 477 362 37
Fragments arranged by size:
644 477 362 337 244 44 37
BseYI C'CCAG_C
Cuts at: 0 31 1180 1198 1317 1520 1806 2145
Size: 31 1149 18 119 203 286 339
Fragments arranged by size:
1149 339 286 203 119 31 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1474 2145
Size: 1474 671
BsiEI CG_ry'CG
Cuts at: 0 1066 1900 2145
Size: 1066 834 245
Fragments arranged by size:
1066 834 245
BsiHKAI G_wGCw'C
Cuts at: 0 264 2145
Size: 264 1881
BsmI GAATG_Cn'
Cuts at: 0 1104 1709 2145
Size: 1104 605 436
Fragments arranged by size:
1104 605 436
Bsp1286I G_dGCh'C
Cuts at: 0 143 264 1199 1306 1351 1583 2145
Size: 143 121 935 107 45 232 562
Fragments arranged by size:
935 562 232 143 121 107 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 141 331 2145
Size: 141 190 1814
Fragments arranged by size:
1814 190 141
BsrBI CCG'CTC
Cuts at: 0 1190 2145
Size: 1190 955
BsrDI GCAATG_nn'
Cuts at: 0 1398 2145
Size: 1398 747
BsrGI T'GTAC_A
Cuts at: 0 1052 2145
Size: 1052 1093
BssSI C'ACGA_G
Cuts at: 0 2085 2145
Size: 2085 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 756 2145
Size: 201 555 1389
Fragments arranged by size:
1389 555 201
BtsI GCAGTG_nn'
Cuts at: 0 143 354 366 396 2145
Size: 143 211 12 30 1749
Fragments arranged by size:
1749 211 143 30 12
ClaI AT'CG_AT
Cuts at: 0 1900 2145
Size: 1900 245
DraIII CAC_nnn'GTG
Cuts at: 0 1620 2145
Size: 1620 525
EaeI y'GGCC_r
Cuts at: 0 176 191 632 1022 1063 1376 2145
Size: 176 15 441 390 41 313 769
Fragments arranged by size:
769 441 390 313 176 41 15
EagI C'GGCC_G
Cuts at: 0 1063 2145
Size: 1063 1082
EarI CTCTTCn'nnn_
Cuts at: 0 94 2145
Size: 94 2051
EcoICRI GAG'CTC
Cuts at: 0 262 2145
Size: 262 1883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 725 919 1095 1266 1412 1615 2017
Size: 168 557 194 176 171 146 203 402
Cuts at: 2017 2145
Size: 128
Fragments arranged by size:
557 402 203 194 176 171 168 146
128
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1868 2057 2145
Size: 1868 189 88
Fragments arranged by size:
1868 189 88
EcoO109I rG'GnC_Cy
Cuts at: 0 1462 1859 2145
Size: 1462 397 286
Fragments arranged by size:
1462 397 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1421 2145
Size: 1421 724
FspI TGC'GCA
Cuts at: 0 304 2145
Size: 304 1841
FspAI rTGC'GCAy
Cuts at: 0 304 2145
Size: 304 1841
HaeII r_GCGC'y
Cuts at: 0 1118 2145
Size: 1118 1027
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 236 901 933 1158 1190 1246 1278
Size: 204 32 665 32 225 32 56 32
Cuts at: 1278 1432 1464 1591 1623 2145
Size: 154 32 127 32 522
Fragments arranged by size:
665 522 225 204 154 127 56 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1718 2145
Size: 1718 427
KpnI G_GTAC'C
Cuts at: 0 2126 2145
Size: 2126 19
MfeI C'AATT_G
Cuts at: 0 782 1157 2145
Size: 782 375 988
Fragments arranged by size:
988 782 375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2042 2145
Size: 2042 103
MscI TGG'CCA
Cuts at: 0 178 193 634 1024 1378 2145
Size: 178 15 441 390 354 767
Fragments arranged by size:
767 441 390 354 178 15
MslI CAynn'nnrTG
Cuts at: 0 199 298 359 2145
Size: 199 99 61 1786
Fragments arranged by size:
1786 199 99 61
MspA1I CmG'CkG
Cuts at: 0 1093 1810 1995 2145
Size: 1093 717 185 150
Fragments arranged by size:
1093 717 185 150
PasI CC'CwG_GG
Cuts at: 0 208 404 1299 2145
Size: 208 196 895 846
Fragments arranged by size:
895 846 208 196
PflMI CCAn_nnn'nTGG
Cuts at: 0 480 1620 2145
Size: 480 1140 525
Fragments arranged by size:
1140 525 480
PfoI T'CCnGG_A
Cuts at: 0 108 1631 2145
Size: 108 1523 514
Fragments arranged by size:
1523 514 108
PmlI CAC'GTG
Cuts at: 0 621 2145
Size: 621 1524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1206 1238 2145
Size: 1206 32 907
Fragments arranged by size:
1206 907 32
PpuMI rG'GwC_Cy
Cuts at: 0 1859 2145
Size: 1859 286
PspXI vC'TCGA_Gb
Cuts at: 0 221 2145
Size: 221 1924
PstI C_TGCA'G
Cuts at: 0 57 138 361 736 924 2145
Size: 57 81 223 375 188 1221
Fragments arranged by size:
1221 375 223 188 81 57
PvuI CG_AT'CG
Cuts at: 0 1900 2145
Size: 1900 245
PvuII CAG'CTG
Cuts at: 0 1093 1810 1995 2145
Size: 1093 717 185 150
Fragments arranged by size:
1093 717 185 150
SacI G_AGCT'C
Cuts at: 0 264 2145
Size: 264 1881
SanDI GG'GwC_CC
Cuts at: 0 1859 2145
Size: 1859 286
SexAI A'CCwGG_T
Cuts at: 0 1929 2145
Size: 1929 216
SfcI C'TryA_G
Cuts at: 0 53 134 357 596 732 920 2007 2145
Size: 53 81 223 239 136 188 1087 138
Fragments arranged by size:
1087 239 223 188 138 136 81 53
SmaI CCC'GGG
Cuts at: 0 1673 2145
Size: 1673 472
SmlI C'TyrA_G
Cuts at: 0 221 1288 2145
Size: 221 1067 857
Fragments arranged by size:
1067 857 221
SpeI A'CTAG_T
Cuts at: 0 1747 2145
Size: 1747 398
StyI C'CwwG_G
Cuts at: 0 1025 2145
Size: 1025 1120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 396 472 762 1559 1992 2145
Size: 396 76 290 797 433 153
Fragments arranged by size:
797 433 396 290 153 76
TatI w'GTAC_w
Cuts at: 0 1052 1989 2145
Size: 1052 937 156
Fragments arranged by size:
1052 937 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 605 637 1652 1684 2145
Size: 605 32 1015 32 461
Fragments arranged by size:
1015 605 461 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 186 1390 2145
Size: 69 117 1204 755
Fragments arranged by size:
1204 755 117 69
XhoI C'TCGA_G
Cuts at: 0 221 2145
Size: 221 1924
XmaI C'CCGG_G
Cuts at: 0 1671 2145
Size: 1671 474
XmnI GAAnn'nnTTC
Cuts at: 0 691 2145
Size: 691 1454
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlfI AlwNI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI
BstXI BtsI ClaI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII KpnI MfeI MmeI MscI MslI MspA1I PasI
PflMI PfoI PmlI PpiI PpuMI PspXI PstI PvuI
PvuII SacI SanDI SexAI SfcI SmaI SmlI SpeI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BfrBI BglII BmgBI BmtI BsaBI BsaWI BsiWI
BsmBI BspEI BspHI BsrFI BssHII BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI CspCI DraI DrdI
EciI EcoRI EcoRV FseI HindIII HpaI KasI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PshAI PsiI PspOMI
PsrI RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SphI SrfI SspI StuI
SwaI Tth111I XbaI ZraI