Restriction map for
pF1KA1738
(Linear) (Six-base) MAPSORT of: KIAA1738.seq Check: 576 from: 1 to: 3381
KIAA1738.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2333 3381
Size: 2333 1048
AccI GT'mk_AC
Cuts at: 0 586 3381
Size: 586 2795
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1189 1204 1660 1949 2797 2945 3381
Size: 959 230 15 456 289 848 148 436
Fragments arranged by size:
959 848 456 436 289 230 148 15
AfeI AGC'GCT
Cuts at: 0 2856 3381
Size: 2856 525
AflII C'TTAA_G
Cuts at: 0 1734 3381
Size: 1734 1647
AflIII A'CryG_T
Cuts at: 0 3162 3381
Size: 3162 219
AhdI GACnn_n'nnGTC
Cuts at: 0 1935 3381
Size: 1935 1446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 3381
Size: 136 32 3213
Fragments arranged by size:
3213 136 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2923 3381
Size: 2923 458
ApaI G_GGCC'C
Cuts at: 0 428 3381
Size: 428 2953
ApaLI G'TGCA_C
Cuts at: 0 67 3381
Size: 67 3314
ApoI r'AATT_y
Cuts at: 0 2736 3381
Size: 2736 645
AvaI C'yCGr_G
Cuts at: 0 89 2722 2813 3381
Size: 89 2633 91 568
Fragments arranged by size:
2633 568 91 89
BanI G'GyrC_C
Cuts at: 0 2216 3381
Size: 2216 1165
BanII G_rGCy'C
Cuts at: 0 428 1127 2383 2594 2891 3381
Size: 428 699 1256 211 297 490
Fragments arranged by size:
1256 699 490 428 297 211
BbsI GAAGACnn'nnnn_
Cuts at: 0 224 407 649 1192 1426 2879 3381
Size: 224 183 242 543 234 1453 502
Fragments arranged by size:
1453 543 502 242 234 224 183
BbvCI CC'TCA_GC
Cuts at: 0 1985 3381
Size: 1985 1396
BciVI GTATCCnnnnn_n'
Cuts at: 0 684 3381
Size: 684 2697
BclI T'GATC_A
Cuts at: 0 2661 3372 3381
Size: 2661 711 9
Fragments arranged by size:
2661 711 9
BglII A'GATC_T
Cuts at: 0 1609 2035 3381
Size: 1609 426 1346
Fragments arranged by size:
1609 1346 426
Bme1580I G_kGCm'C
Cuts at: 0 71 428 842 1010 2219 3381
Size: 71 357 414 168 1209 1162
Fragments arranged by size:
1209 1162 414 357 168 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 3381
Size: 437 2944
BmtI G_CTAG'C
Cuts at: 0 437 3381
Size: 437 2944
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1404 2865 3381
Size: 1404 1461 516
Fragments arranged by size:
1461 1404 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 2183 2623 2835 3265 3381
Size: 1225 958 440 212 430 116
Fragments arranged by size:
1225 958 440 430 212 116
Bpu10I CC'TnA_GC
Cuts at: 0 429 1985 3381
Size: 429 1556 1396
Fragments arranged by size:
1556 1396 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 1741 3381
Size: 1315 426 1640
Fragments arranged by size:
1640 1315 426
BsaI GGTCTCn'nnnn_
Cuts at: 0 2719 3381
Size: 2719 662
BsaAI yAC'GTr
Cuts at: 0 72 3381
Size: 72 3309
BsaHI Gr'CG_yC
Cuts at: 0 876 3381
Size: 876 2505
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 1033 1063 2251 2281 2897 2927
Size: 136 30 867 30 1188 30 616 30
Cuts at: 2927 3238 3268 3381
Size: 311 30 113
Fragments arranged by size:
1188 867 616 311 136 113 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 145 148 157 160 163 184 1057
Size: 142 3 3 9 3 3 21 873
Cuts at: 1057 1060 2272 2391 2461 2853 2902 3381
Size: 3 1212 119 70 392 49 479
Fragments arranged by size:
1212 873 479 392 142 119 70 49
21 9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2413 2890 3381
Size: 2413 477 491
Fragments arranged by size:
2413 491 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1417 2171 3381
Size: 94 1323 754 1210
Fragments arranged by size:
1323 1210 754 94
BsiEI CG_ry'CG
Cuts at: 0 590 1394 3381
Size: 590 804 1987
Fragments arranged by size:
1987 804 590
BsiHKAI G_wGCw'C
Cuts at: 0 71 1346 1563 2594 3381
Size: 71 1275 217 1031 787
Fragments arranged by size:
1275 1031 787 217 71
BsmI GAATG_Cn'
Cuts at: 0 2478 2818 3381
Size: 2478 340 563
Fragments arranged by size:
2478 563 340
BsmBI CGTCTCn'nnnn_
Cuts at: 0 330 3381
Size: 330 3051
Bsp1286I G_dGCh'C
Cuts at: 0 71 428 842 1010 1127 1346 1563 2219
Size: 71 357 414 168 117 219 217 656
Cuts at: 2219 2383 2594 2891 3381
Size: 164 211 297 490
Fragments arranged by size:
656 490 414 357 297 219 217 211
168 164 117 71
BspHI T'CATG_A
Cuts at: 0 563 1517 3381
Size: 563 954 1864
Fragments arranged by size:
1864 954 563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2333 3381
Size: 2333 1048
BsrFI r'CCGG_y
Cuts at: 0 256 3381
Size: 256 3125
BsrGI T'GTAC_A
Cuts at: 0 1262 2333 3381
Size: 1262 1071 1048
Fragments arranged by size:
1262 1071 1048
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2313 3381
Size: 2313 1068
BstEII G'GTnAC_C
Cuts at: 0 1800 3381
Size: 1800 1581
BstYI r'GATC_y
Cuts at: 0 1609 2035 2543 3381
Size: 1609 426 508 838
Fragments arranged by size:
1609 838 508 426
Bsu36I CC'TnA_GG
Cuts at: 0 2021 3381
Size: 2021 1360
BtgI C'CryG_G
Cuts at: 0 665 3317 3381
Size: 665 2652 64
Fragments arranged by size:
2652 665 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 2852 3381
Size: 84 2768 529
Fragments arranged by size:
2768 529 84
BtsI GCAGTG_nn'
Cuts at: 0 2256 3381
Size: 2256 1125
DraI TTT'AAA
Cuts at: 0 834 3381
Size: 834 2547
DraIII CAC_nnn'GTG
Cuts at: 0 1482 1558 3381
Size: 1482 76 1823
Fragments arranged by size:
1823 1482 76
DrdI GACnn_nn'nnGTC
Cuts at: 0 2204 3381
Size: 2204 1177
EaeI y'GGCC_r
Cuts at: 0 243 1391 2079 3181 3381
Size: 243 1148 688 1102 200
Fragments arranged by size:
1148 1102 688 243 200
EagI C'GGCC_G
Cuts at: 0 1391 3381
Size: 1391 1990
EarI CTCTTCn'nnn_
Cuts at: 0 134 1241 1964 3381
Size: 134 1107 723 1417
Fragments arranged by size:
1417 1107 723 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2845 3381
Size: 2845 536
EcoICRI GAG'CTC
Cuts at: 0 2592 3381
Size: 2592 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1189 1204 1225 1660 1949 2183 2623
Size: 959 230 15 21 435 289 234 440
Cuts at: 2623 2797 2835 2945 3265 3381
Size: 174 38 110 320 116
Fragments arranged by size:
959 440 435 320 289 234 230 174
116 110 38 21 15
EcoO109I rG'GnC_Cy
Cuts at: 0 306 424 425 1998 2705 2810 3381
Size: 306 118 1 1573 707 105 571
Fragments arranged by size:
1573 707 571 306 118 105 1
EcoRV GAT'ATC
Cuts at: 0 1620 3381
Size: 1620 1761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2779 3381
Size: 2779 602
HaeII r_GCGC'y
Cuts at: 0 2858 3381
Size: 2858 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 83 115 277 309 682 714 1179 1211
Size: 83 32 162 32 373 32 465 32
Cuts at: 1211 1300 1332 1921 1953 2046 2078 2251 2283
Size: 89 32 589 32 93 32 173 32
Cuts at: 2283 2518 2550 2830 2862 3313 3345 3381
Size: 235 32 280 32 451 32 36
Fragments arranged by size:
589 465 451 373 280 235 173 162
93 89 83 36 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 587 3108 3381
Size: 587 2521 273
Fragments arranged by size:
2521 587 273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 668 1109 2114 2763 2916 3074 3381
Size: 329 339 441 1005 649 153 158 307
Fragments arranged by size:
1005 649 441 339 329 307 158 153
MscI TGG'CCA
Cuts at: 0 245 3381
Size: 245 3136
MslI CAynn'nnrTG
Cuts at: 0 40 2617 3304 3381
Size: 40 2577 687 77
Fragments arranged by size:
2577 687 77 40
MspA1I CmG'CkG
Cuts at: 0 1984 2413 2894 3381
Size: 1984 429 481 487
Fragments arranged by size:
1984 487 481 429
NaeI GCC'GGC
Cuts at: 0 258 3381
Size: 258 3123
NcoI C'CATG_G
Cuts at: 0 3317 3381
Size: 3317 64
NgoMIV G'CCGG_C
Cuts at: 0 256 3381
Size: 256 3125
NheI G'CTAG_C
Cuts at: 0 433 3381
Size: 433 2948
NspI r_CATG'y
Cuts at: 0 45 3166 3381
Size: 45 3121 215
Fragments arranged by size:
3121 215 45
PasI CC'CwG_GG
Cuts at: 0 406 1155 2917 3381
Size: 406 749 1762 464
Fragments arranged by size:
1762 749 464 406
PciI A'CATG_T
Cuts at: 0 3162 3381
Size: 3162 219
PflMI CCAn_nnn'nTGG
Cuts at: 0 3190 3381
Size: 3190 191
PfoI T'CCnGG_A
Cuts at: 0 1765 3381
Size: 1765 1616
PmlI CAC'GTG
Cuts at: 0 72 3381
Size: 72 3309
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 3381
Size: 136 32 3213
Fragments arranged by size:
3213 136 32
PpuMI rG'GwC_Cy
Cuts at: 0 1998 2705 3381
Size: 1998 707 676
Fragments arranged by size:
1998 707 676
PshAI GACnn'nnGTC
Cuts at: 0 649 3381
Size: 649 2732
PsiI TTA'TAA
Cuts at: 0 1438 3381
Size: 1438 1943
PspOMI G'GGCC_C
Cuts at: 0 424 3381
Size: 424 2957
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1619 1651 3381
Size: 1619 32 1730
Fragments arranged by size:
1730 1619 32
PstI C_TGCA'G
Cuts at: 0 1527 2390 3381
Size: 1527 863 991
Fragments arranged by size:
1527 991 863
PvuII CAG'CTG
Cuts at: 0 1984 2413 3381
Size: 1984 429 968
Fragments arranged by size:
1984 968 429
SacI G_AGCT'C
Cuts at: 0 2594 3381
Size: 2594 787
SalI G'TCGA_C
Cuts at: 0 585 3381
Size: 585 2796
ScaI AGT'ACT
Cuts at: 0 163 3381
Size: 163 3218
SfcI C'TryA_G
Cuts at: 0 1029 1523 2325 2386 3381
Size: 1029 494 802 61 995
Fragments arranged by size:
1029 995 802 494 61
SmaI CCC'GGG
Cuts at: 0 2815 3381
Size: 2815 566
SmlI C'TyrA_G
Cuts at: 0 1330 1720 1734 2722 3381
Size: 1330 390 14 988 659
Fragments arranged by size:
1330 988 659 390 14
SspI AAT'ATT
Cuts at: 0 2492 2995 3381
Size: 2492 503 386
Fragments arranged by size:
2492 503 386
StuI AGG'CCT
Cuts at: 0 1181 2644 3381
Size: 1181 1463 737
Fragments arranged by size:
1463 1181 737
StyI C'CwwG_G
Cuts at: 0 212 1509 2667 3098 3317 3381
Size: 212 1297 1158 431 219 64
Fragments arranged by size:
1297 1158 431 219 212 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 1121 2457 3146 3381
Size: 604 517 1336 689 235
Fragments arranged by size:
1336 689 604 517 235
TatI w'GTAC_w
Cuts at: 0 161 382 1262 2333 3078 3381
Size: 161 221 880 1071 745 303
Fragments arranged by size:
1071 880 745 303 221 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 1033 1065 1096 1128 3381
Size: 281 32 720 32 31 32 2253
Fragments arranged by size:
2253 720 281 32 32 32 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1483 3381
Size: 1483 1898
XhoI C'TCGA_G
Cuts at: 0 2722 3381
Size: 2722 659
XmaI C'CCGG_G
Cuts at: 0 2813 3381
Size: 2813 568
XmnI GAAnn'nnTTC
Cuts at: 0 1080 3381
Size: 1080 2301
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI
BbvCI BciVI BclI BglII Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrFI BsrGI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SacI
SalI ScaI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AleI AlfI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglI
BlpI BmgBI BsaBI BsaWI BsiWI BspEI BsrBI BsrDI
BssHII BssSI BstBI BstXI BstZ17I ClaI CspCI EcoNI
EcoRI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NotI NruI NsiI PacI
PmeI PspXI PvuI RsrII SacII SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI ZraI