Restriction map for
pF1KSDA1736
(Linear) (Six-base) MAPSORT of: KIAA1736.seq Check: 8519 from: 1 to: 3714
KIAA1736.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 498 3714
Size: 498 3216
Acc65I G'GTAC_C
Cuts at: 0 3529 3714
Size: 3529 185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 1095 1376 1480 1522 2739 3073 3130
Size: 165 930 281 104 42 1217 334 57
Cuts at: 3130 3714
Size: 584
Fragments arranged by size:
1217 930 584 334 281 165 104 57
42
AfeI AGC'GCT
Cuts at: 0 3606 3714
Size: 3606 108
AflIII A'CryG_T
Cuts at: 0 2841 3714
Size: 2841 873
AhdI GACnn_n'nnGTC
Cuts at: 0 877 2230 3714
Size: 877 1353 1484
Fragments arranged by size:
1484 1353 877
AleI CACnn'nnGTG
Cuts at: 0 214 1658 3112 3714
Size: 214 1444 1454 602
Fragments arranged by size:
1454 1444 602 214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 809 1861 3714
Size: 809 1052 1853
Fragments arranged by size:
1853 1052 809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 620 652 3714
Size: 620 32 3062
Fragments arranged by size:
3062 620 32
AlwNI CAG_nnn'CTG
Cuts at: 0 93 453 938 1249 1545 1557 2529 3138
Size: 93 360 485 311 296 12 972 609
Cuts at: 3138 3214 3279 3714
Size: 76 65 435
Fragments arranged by size:
972 609 485 435 360 311 296 93
76 65 12
ApoI r'AATT_y
Cuts at: 0 1976 3714
Size: 1976 1738
AvaI C'yCGr_G
Cuts at: 0 59 950 1473 1855 3452 3714
Size: 59 891 523 382 1597 262
Fragments arranged by size:
1597 891 523 382 262 59
AvrII C'CTAG_G
Cuts at: 0 3691 3714
Size: 3691 23
BamHI G'GATC_C
Cuts at: 0 917 2122 2711 3269 3714
Size: 917 1205 589 558 445
Fragments arranged by size:
1205 917 589 558 445
BanI G'GyrC_C
Cuts at: 0 2180 3127 3183 3241 3415 3529 3714
Size: 2180 947 56 58 174 114 185
Fragments arranged by size:
2180 947 185 174 114 58 56
BanII G_rGCy'C
Cuts at: 0 75 1043 2050 2190 3714
Size: 75 968 1007 140 1524
Fragments arranged by size:
1524 1007 968 140 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 529 2745 2979 3714
Size: 253 276 2216 234 735
Fragments arranged by size:
2216 735 276 253 234
BbvCI CC'TCA_GC
Cuts at: 0 824 3608 3714
Size: 824 2784 106
Fragments arranged by size:
2784 824 106
BciVI GTATCCnnnnn_n'
Cuts at: 0 26 3714
Size: 26 3688
BglI GCCn_nnn'nGGC
Cuts at: 0 427 2558 3714
Size: 427 2131 1156
Fragments arranged by size:
2131 1156 427
BglII A'GATC_T
Cuts at: 0 1996 2483 3714
Size: 1996 487 1231
Fragments arranged by size:
1996 1231 487
BlpI GC'TnA_GC
Cuts at: 0 74 446 3262 3714
Size: 74 372 2816 452
Fragments arranged by size:
2816 452 372 74
Bme1580I G_kGCm'C
Cuts at: 0 681 3130 3406 3714
Size: 681 2449 276 308
Fragments arranged by size:
2449 681 308 276
BmrI ACTGGGnnnn_n'
Cuts at: 0 1977 1980 2973 3714
Size: 1977 3 993 741
Fragments arranged by size:
1977 993 741 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1080 1686 2064 3714
Size: 1080 606 378 1650
Fragments arranged by size:
1650 1080 606 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1443 1543 1869 1915 2389 3714
Size: 513 930 100 326 46 474 1325
Fragments arranged by size:
1325 930 513 474 326 100 46
Bpu10I CC'TnA_GC
Cuts at: 0 824 2873 3496 3608 3714
Size: 824 2049 623 112 106
Fragments arranged by size:
2049 824 623 112 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 2776 2906 3714
Size: 2776 130 808
Fragments arranged by size:
2776 808 130
BsaHI Gr'CG_yC
Cuts at: 0 709 3714
Size: 709 3005
BsaWI w'CCGG_w
Cuts at: 0 29 3714
Size: 29 3685
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2903 2933 3605 3635 3714
Size: 2903 30 672 30 79
Fragments arranged by size:
2903 672 79 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 1764 1939 2056 2068 2085 2808 3420
Size: 706 1058 175 117 12 17 723 612
Cuts at: 3420 3714
Size: 294
Fragments arranged by size:
1058 723 706 612 294 175 117 17
12
BseYI C'CCAG_C
Cuts at: 0 1748 1807 1879 2151 2272 2545 3437 3551
Size: 1748 59 72 272 121 273 892 114
Cuts at: 3551 3601 3714
Size: 50 113
Fragments arranged by size:
1748 892 273 272 121 114 113 72
59 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 2195 2491 2704 3331 3714
Size: 715 1480 296 213 627 383
Fragments arranged by size:
1480 715 627 383 296 213
BsiEI CG_ry'CG
Cuts at: 0 1102 3714
Size: 1102 2612
BsiHKAI G_wGCw'C
Cuts at: 0 60 75 2050 2135 2190 2738 3328 3714
Size: 60 15 1975 85 55 548 590 386
Fragments arranged by size:
1975 590 548 386 85 60 55 15
BsmI GAATG_Cn'
Cuts at: 0 27 1481 3081 3162 3380 3714
Size: 27 1454 1600 81 218 334
Fragments arranged by size:
1600 1454 334 218 81 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1503 2974 3714
Size: 1503 1471 740
Fragments arranged by size:
1503 1471 740
Bsp1286I G_dGCh'C
Cuts at: 0 60 75 681 1043 2050 2135 2190 2738
Size: 60 15 606 362 1007 85 55 548
Cuts at: 2738 3130 3328 3406 3714
Size: 392 198 78 308
Fragments arranged by size:
1007 606 548 392 362 308 198 85
78 60 55 15
BspEI T'CCGG_A
Cuts at: 0 29 3714
Size: 29 3685
BspHI T'CATG_A
Cuts at: 0 2283 3714
Size: 2283 1431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1791 3714
Size: 1791 1923
BsrBI CCG'CTC
Cuts at: 0 749 1178 1286 1860 3021 3714
Size: 749 429 108 574 1161 693
Fragments arranged by size:
1161 749 693 574 429 108
BsrDI GCAATG_nn'
Cuts at: 0 416 2202 2381 3714
Size: 416 1786 179 1333
Fragments arranged by size:
1786 1333 416 179
BsrFI r'CCGG_y
Cuts at: 0 1097 1135 1532 2399 3714
Size: 1097 38 397 867 1315
Fragments arranged by size:
1315 1097 867 397 38
BsrGI T'GTAC_A
Cuts at: 0 1029 1385 3714
Size: 1029 356 2329
Fragments arranged by size:
2329 1029 356
BstAPI GCAn_nnn'nTGC
Cuts at: 0 938 3714
Size: 938 2776
BstXI CCAn_nnnn'nTGG
Cuts at: 0 913 1893 3714
Size: 913 980 1821
Fragments arranged by size:
1821 980 913
BstYI r'GATC_y
Cuts at: 0 476 635 917 1246 1996 2122 2173 2483
Size: 476 159 282 329 750 126 51 310
Cuts at: 2483 2711 3269 3714
Size: 228 558 445
Fragments arranged by size:
750 558 476 445 329 310 282 228
159 126 51
Bsu36I CC'TnA_GG
Cuts at: 0 2039 3207 3714
Size: 2039 1168 507
Fragments arranged by size:
2039 1168 507
BtgI C'CryG_G
Cuts at: 0 65 292 833 1683 2772 3305 3332 3341
Size: 65 227 541 850 1089 533 27 9
Cuts at: 3341 3703 3714
Size: 362 11
Fragments arranged by size:
1089 850 541 533 362 227 65 27
11 9
BtsI GCAGTG_nn'
Cuts at: 0 390 464 1130 1665 2351 2591 3624 3714
Size: 390 74 666 535 686 240 1033 90
Fragments arranged by size:
1033 686 666 535 390 240 90 74
DraIII CAC_nnn'GTG
Cuts at: 0 2742 3714
Size: 2742 972
EaeI y'GGCC_r
Cuts at: 0 1099 1686 2372 2520 3714
Size: 1099 587 686 148 1194
Fragments arranged by size:
1194 1099 686 587 148
EagI C'GGCC_G
Cuts at: 0 1099 3714
Size: 1099 2615
EarI CTCTTCn'nnn_
Cuts at: 0 756 1332 2322 3573 3714
Size: 756 576 990 1251 141
Fragments arranged by size:
1251 990 756 576 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1309 3301 3714
Size: 1309 1992 413
Fragments arranged by size:
1992 1309 413
EcoICRI GAG'CTC
Cuts at: 0 73 2048 2188 3714
Size: 73 1975 140 1526
Fragments arranged by size:
1975 1526 140 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 513 1095 1376 1443 1480 1522 1543
Size: 165 348 582 281 67 37 42 21
Cuts at: 1543 1869 1915 2389 2739 3073 3130 3714
Size: 326 46 474 350 334 57 584
Fragments arranged by size:
584 582 474 350 348 334 326 281
165 67 57 46 42 37 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2570 2706 2790 2876 3218 3714
Size: 2570 136 84 86 342 496
Fragments arranged by size:
2570 496 342 136 86 84
EcoO109I rG'GnC_Cy
Cuts at: 0 1086 1165 1189 1630 1803 2331 3211 3688
Size: 1086 79 24 441 173 528 880 477
Cuts at: 3688 3714
Size: 26
Fragments arranged by size:
1086 880 528 477 441 173 79 26
24
HaeII r_GCGC'y
Cuts at: 0 843 3608 3714
Size: 843 2765 106
Fragments arranged by size:
2765 843 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 549 581 863 895 1282 1314 2216 2248
Size: 549 32 282 32 387 32 902 32
Cuts at: 2248 2347 2379 3605 3637 3714
Size: 99 32 1226 32 77
Fragments arranged by size:
1226 902 549 387 282 99 77 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 499 600 1070 1241 2325 3635 3714
Size: 499 101 470 171 1084 1310 79
Fragments arranged by size:
1310 1084 499 470 171 101 79
HpaI GTT'AAC
Cuts at: 0 600 2325 3714
Size: 600 1725 1389
Fragments arranged by size:
1725 1389 600
KpnI G_GTAC'C
Cuts at: 0 3533 3714
Size: 3533 181
MfeI C'AATT_G
Cuts at: 0 593 3714
Size: 593 3121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1697 3026 3714
Size: 894 803 1329 688
Fragments arranged by size:
1329 894 803 688
MscI TGG'CCA
Cuts at: 0 1688 2374 2522 3714
Size: 1688 686 148 1192
Fragments arranged by size:
1688 1192 686 148
MslI CAynn'nnrTG
Cuts at: 0 214 763 1658 2288 2656 3112 3331 3714
Size: 214 549 895 630 368 456 219 383
Fragments arranged by size:
895 630 549 456 383 368 219 214
MspA1I CmG'CkG
Cuts at: 0 78 876 895 938 2501 2835 2973 3150
Size: 78 798 19 43 1563 334 138 177
Cuts at: 3150 3315 3714
Size: 165 399
Fragments arranged by size:
1563 798 399 334 177 165 138 78
43 19
NaeI GCC'GGC
Cuts at: 0 1137 3714
Size: 1137 2577
NcoI C'CATG_G
Cuts at: 0 65 292 2772 3332 3341 3714
Size: 65 227 2480 560 9 373
Fragments arranged by size:
2480 560 373 227 65 9
NgoMIV G'CCGG_C
Cuts at: 0 1135 3714
Size: 1135 2579
NspI r_CATG'y
Cuts at: 0 738 2845 3714
Size: 738 2107 869
Fragments arranged by size:
2107 869 738
PasI CC'CwG_GG
Cuts at: 0 3535 3714
Size: 3535 179
PciI A'CATG_T
Cuts at: 0 2841 3714
Size: 2841 873
PflMI CCAn_nnn'nTGG
Cuts at: 0 1834 2529 2610 2884 3138 3654 3714
Size: 1834 695 81 274 254 516 60
Fragments arranged by size:
1834 695 516 274 254 81 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 3714
Size: 1270 32 2412
Fragments arranged by size:
2412 1270 32
PpuMI rG'GwC_Cy
Cuts at: 0 1165 1803 2331 3211 3714
Size: 1165 638 528 880 503
Fragments arranged by size:
1165 880 638 528 503
PshAI GACnn'nnGTC
Cuts at: 0 494 3714
Size: 494 3220
PspXI vC'TCGA_Gb
Cuts at: 0 950 3714
Size: 950 2764
PstI C_TGCA'G
Cuts at: 0 89 2517 3155 3687 3714
Size: 89 2428 638 532 27
Fragments arranged by size:
2428 638 532 89 27
PvuII CAG'CTG
Cuts at: 0 895 2501 2973 3150 3714
Size: 895 1606 472 177 564
Fragments arranged by size:
1606 895 564 472 177
SacI G_AGCT'C
Cuts at: 0 75 2050 2190 3714
Size: 75 1975 140 1524
Fragments arranged by size:
1975 1524 140 75
SalI G'TCGA_C
Cuts at: 0 497 3714
Size: 497 3217
SapI GCTCTTCn'nnn_
Cuts at: 0 756 3714
Size: 756 2958
ScaI AGT'ACT
Cuts at: 0 1268 2260 2959 3714
Size: 1268 992 699 755
Fragments arranged by size:
1268 992 755 699
SexAI A'CCwGG_T
Cuts at: 0 2915 3411 3714
Size: 2915 496 303
Fragments arranged by size:
2915 496 303
SfcI C'TryA_G
Cuts at: 0 85 2513 3151 3683 3714
Size: 85 2428 638 532 31
Fragments arranged by size:
2428 638 532 85 31
SmlI C'TyrA_G
Cuts at: 0 950 1855 3714
Size: 950 905 1859
Fragments arranged by size:
1859 950 905
StuI AGG'CCT
Cuts at: 0 326 1063 2020 2938 3494 3714
Size: 326 737 957 918 556 220
Fragments arranged by size:
957 918 737 556 326 220
StyI C'CwwG_G
Cuts at: 0 65 292 912 2236 2772 3332 3341 3691
Size: 65 227 620 1324 536 560 9 350
Cuts at: 3691 3714
Size: 23
Fragments arranged by size:
1324 620 560 536 350 227 65 23
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 1051 1999 2150 3145 3714
Size: 226 825 948 151 995 569
Fragments arranged by size:
995 948 825 569 226 151
TatI w'GTAC_w
Cuts at: 0 1029 1114 1266 1385 2258 2588 2957 3714
Size: 1029 85 152 119 873 330 369 757
Fragments arranged by size:
1029 873 757 369 330 152 119 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 294 326 724 756 939 971 2696 2728
Size: 294 32 398 32 183 32 1725 32
Cuts at: 2728 2903 2935 3714
Size: 175 32 779
Fragments arranged by size:
1725 779 398 294 183 175 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 982 3714
Size: 982 2732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 2736 3339 3655 3714
Size: 423 2313 603 316 59
Fragments arranged by size:
2313 603 423 316 59
XhoI C'TCGA_G
Cuts at: 0 950 1855 3714
Size: 950 905 1859
Fragments arranged by size:
1859 950 905
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApoI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
HpaI KpnI MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PflMI PpiI PpuMI
PshAI PspXI PstI PvuII SacI SalI SapI ScaI
SexAI SfcI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI ApaI ApaLI AscI
AseI AsiSI BaeI BbeI BcgI BclI BfrBI BmgBI
BmtI BpuEI BsaAI BsaBI BsiWI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI FspI FspAI HindIII KasI MluI
NarI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PsiI PspOMI PsrI PvuI RsrII SacII
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XmaI XmnI
ZraI