(Linear) (Six-base) MAPSORT of: KIAA1732.seq Check: 9271 from: 1 to: 7926 KIAA1732.orf With 182 enzymes: SgfI * October 24, 2006 15:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 594 6396 7926 Size: 594 5802 1530 Fragments arranged by size: 5802 1530 594 AccI GT'mk_AC Cuts at: 0 329 548 3094 3684 3933 7926 Size: 329 219 2546 590 249 3993 Fragments arranged by size: 3993 2546 590 329 249 219 AclI AA'CG_TT Cuts at: 0 5221 7926 Size: 5221 2705 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 89 213 307 962 1547 2426 2441 2632 Size: 89 124 94 655 585 879 15 191 Cuts at: 2632 4063 4246 4344 4846 5189 5554 5605 6357 Size: 1431 183 98 502 343 365 51 752 Cuts at: 6357 7201 7760 7816 7926 Size: 844 559 56 110 Fragments arranged by size: 1431 879 844 752 655 585 559 502 365 343 191 183 124 110 98 94 89 56 51 15 AflIII A'CryG_T Cuts at: 0 795 2964 3732 3886 6915 7135 7926 Size: 795 2169 768 154 3029 220 791 Fragments arranged by size: 3029 2169 795 791 768 220 154 AhdI GACnn_n'nnGTC Cuts at: 0 4092 7926 Size: 4092 3834 AleI CACnn'nnGTG Cuts at: 0 5123 7926 Size: 5123 2803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2073 7926 Size: 2073 5853 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1598 1630 7926 Size: 1598 32 6296 Fragments arranged by size: 6296 1598 32 AlwNI CAG_nnn'CTG Cuts at: 0 967 2041 2587 5836 6100 6663 6928 7689 Size: 967 1074 546 3249 264 563 265 761 Cuts at: 7689 7926 Size: 237 Fragments arranged by size: 3249 1074 967 761 563 546 265 264 237 ApaI G_GGCC'C Cuts at: 0 4340 7926 Size: 4340 3586 ApoI r'AATT_y Cuts at: 0 422 872 1018 1702 1710 1915 2517 2536 Size: 422 450 146 684 8 205 602 19 Cuts at: 2536 2898 3015 3222 3346 3425 3552 4063 6305 Size: 362 117 207 124 79 127 511 2242 Cuts at: 6305 7926 Size: 1621 Fragments arranged by size: 2242 1621 684 602 511 450 422 362 207 205 146 127 124 117 79 19 8 AseI AT'TA_AT Cuts at: 0 1808 6155 7926 Size: 1808 4347 1771 Fragments arranged by size: 4347 1808 1771 AvaI C'yCGr_G Cuts at: 0 4772 7427 7926 Size: 4772 2655 499 Fragments arranged by size: 4772 2655 499 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2330 2363 3838 3871 7926 Size: 2330 33 1475 33 4055 Fragments arranged by size: 4055 2330 1475 33 33 BamHI G'GATC_C Cuts at: 0 3918 4036 6765 7485 7926 Size: 3918 118 2729 720 441 Fragments arranged by size: 3918 2729 720 441 118 BanI G'GyrC_C Cuts at: 0 172 4961 6909 7926 Size: 172 4789 1948 1017 Fragments arranged by size: 4789 1948 1017 172 BanII G_rGCy'C Cuts at: 0 1063 1312 3764 4340 4530 6119 7926 Size: 1063 249 2452 576 190 1589 1807 Fragments arranged by size: 2452 1807 1589 1063 576 249 190 BbeI G_GCGC'C Cuts at: 0 4965 7926 Size: 4965 2961 BbsI GAAGACnn'nnnn_ Cuts at: 0 1405 2647 2931 3035 4561 6403 7207 7426 Size: 1405 1242 284 104 1526 1842 804 219 Cuts at: 7426 7748 7926 Size: 322 178 Fragments arranged by size: 1842 1526 1405 1242 804 322 284 219 178 104 BbvCI CC'TCA_GC Cuts at: 0 4933 7316 7926 Size: 4933 2383 610 Fragments arranged by size: 4933 2383 610 BclI T'GATC_A Cuts at: 0 1980 5961 6843 7463 7926 Size: 1980 3981 882 620 463 Fragments arranged by size: 3981 1980 882 620 463 BfrBI ATG'CAT Cuts at: 0 2141 3052 7926 Size: 2141 911 4874 Fragments arranged by size: 4874 2141 911 BglII A'GATC_T Cuts at: 0 706 1210 4881 7926 Size: 706 504 3671 3045 Fragments arranged by size: 3671 3045 706 504 BlpI GC'TnA_GC Cuts at: 0 1062 5440 5824 6851 7926 Size: 1062 4378 384 1027 1075 Fragments arranged by size: 4378 1075 1062 1027 384 Bme1580I G_kGCm'C Cuts at: 0 4316 4340 7926 Size: 4316 24 3586 Fragments arranged by size: 4316 3586 24 BmrI ACTGGGnnnn_n' Cuts at: 0 1618 3697 3924 3956 6814 6942 7018 7665 Size: 1618 2079 227 32 2858 128 76 647 Cuts at: 7665 7926 Size: 261 Fragments arranged by size: 2858 2079 1618 647 261 227 128 76 32 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1229 1251 4765 4866 5436 7926 Size: 1229 22 3514 101 570 2490 Fragments arranged by size: 3514 2490 1229 570 101 22 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 293 1826 2699 3181 4886 6972 7605 7843 Size: 293 1533 873 482 1705 2086 633 238 Cuts at: 7843 7926 Size: 83 Fragments arranged by size: 2086 1705 1533 873 633 482 293 238 83 Bpu10I CC'TnA_GC Cuts at: 0 4933 7316 7926 Size: 4933 2383 610 Fragments arranged by size: 4933 2383 610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1392 2870 3773 3953 4514 4710 7091 7546 Size: 1392 1478 903 180 561 196 2381 455 Cuts at: 7546 7926 Size: 380 Fragments arranged by size: 2381 1478 1392 903 561 455 380 196 180 BsaI GGTCTCn'nnnn_ Cuts at: 0 1436 1486 1844 5039 5422 5720 6416 7926 Size: 1436 50 358 3195 383 298 696 1510 Fragments arranged by size: 3195 1510 1436 696 383 358 298 50 BsaAI yAC'GTr Cuts at: 0 798 3809 3889 7926 Size: 798 3011 80 4037 Fragments arranged by size: 4037 3011 798 80 BsaBI GATnn'nnATC Cuts at: 0 1182 2609 5960 7926 Size: 1182 1427 3351 1966 Fragments arranged by size: 3351 1966 1427 1182 BsaHI Gr'CG_yC Cuts at: 0 4962 7926 Size: 4962 2964 BsaWI w'CCGG_w Cuts at: 0 3420 7700 7926 Size: 3420 4280 226 Fragments arranged by size: 4280 3420 226 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2616 2646 2951 2981 5832 5862 6002 6032 Size: 2616 30 305 30 2851 30 140 30 Cuts at: 6032 6178 6208 6970 7000 7253 7283 7783 7813 Size: 146 30 762 30 253 30 500 30 Cuts at: 7813 7926 Size: 113 Fragments arranged by size: 2851 2616 762 500 305 253 146 140 113 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2966 4027 5318 6199 7370 7926 Size: 2966 1061 1291 881 1171 556 Fragments arranged by size: 2966 1291 1171 1061 881 556 BseYI C'CCAG_C Cuts at: 0 1834 4682 4949 6769 6808 7205 7926 Size: 1834 2848 267 1820 39 397 721 Fragments arranged by size: 2848 1834 1820 721 397 267 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 254 4891 7082 7926 Size: 124 130 4637 2191 844 Fragments arranged by size: 4637 2191 844 130 124 BsiEI CG_ry'CG Cuts at: 0 5601 7926 Size: 5601 2325 BsiHKAI G_wGCw'C Cuts at: 0 59 1063 1312 1365 3764 4530 5441 5787 Size: 59 1004 249 53 2399 766 911 346 Cuts at: 5787 5830 6797 7926 Size: 43 967 1129 Fragments arranged by size: 2399 1129 1004 967 911 766 346 249 59 53 43 BsmI GAATG_Cn' Cuts at: 0 4493 6043 7926 Size: 4493 1550 1883 Fragments arranged by size: 4493 1883 1550 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3199 4831 4997 7926 Size: 3199 1632 166 2929 Fragments arranged by size: 3199 2929 1632 166 Bsp1286I G_dGCh'C Cuts at: 0 59 1063 1312 1365 3764 4316 4340 4530 Size: 59 1004 249 53 2399 552 24 190 Cuts at: 4530 5441 5787 5830 6119 6797 7926 Size: 911 346 43 289 678 1129 Fragments arranged by size: 2399 1129 1004 911 678 552 346 289 249 190 59 53 43 24 BspEI T'CCGG_A Cuts at: 0 3420 7926 Size: 3420 4506 BspHI T'CATG_A Cuts at: 0 3102 4574 5111 7926 Size: 3102 1472 537 2815 Fragments arranged by size: 3102 2815 1472 537 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 594 2212 6396 7926 Size: 594 1618 4184 1530 Fragments arranged by size: 4184 1618 1530 594 BsrBI CCG'CTC Cuts at: 0 6501 7926 Size: 6501 1425 BsrDI GCAATG_nn' Cuts at: 0 285 3912 7836 7926 Size: 285 3627 3924 90 Fragments arranged by size: 3924 3627 285 90 BsrFI r'CCGG_y Cuts at: 0 7011 7926 Size: 7011 915 BsrGI T'GTAC_A Cuts at: 0 1723 4501 7077 7191 7926 Size: 1723 2778 2576 114 735 Fragments arranged by size: 2778 2576 1723 735 114 BssSI C'ACGA_G Cuts at: 0 1364 7926 Size: 1364 6562 BstAPI GCAn_nnn'nTGC Cuts at: 0 4902 6654 7926 Size: 4902 1752 1272 Fragments arranged by size: 4902 1752 1272 BstBI TT'CG_AA Cuts at: 0 4714 7926 Size: 4714 3212 BstEII G'GTnAC_C Cuts at: 0 3544 5293 6730 7926 Size: 3544 1749 1437 1196 Fragments arranged by size: 3544 1749 1437 1196 BstXI CCAn_nnnn'nTGG Cuts at: 0 4376 5196 5245 6270 6967 7926 Size: 4376 820 49 1025 697 959 Fragments arranged by size: 4376 1025 959 820 697 49 BstYI r'GATC_y Cuts at: 0 706 1083 1210 2023 3918 4036 4881 5573 Size: 706 377 127 813 1895 118 845 692 Cuts at: 5573 5955 6765 7362 7431 7485 7926 Size: 382 810 597 69 54 441 Fragments arranged by size: 1895 845 813 810 706 692 597 441 382 377 127 118 69 54 Bsu36I CC'TnA_GG Cuts at: 0 1447 7814 7926 Size: 1447 6367 112 Fragments arranged by size: 6367 1447 112 BtgI C'CryG_G Cuts at: 0 3023 3236 7926 Size: 3023 213 4690 Fragments arranged by size: 4690 3023 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4389 5762 7926 Size: 4389 1373 2164 Fragments arranged by size: 4389 2164 1373 BtsI GCAGTG_nn' Cuts at: 0 266 463 530 2735 4498 4879 4891 6679 Size: 266 197 67 2205 1763 381 12 1788 Cuts at: 6679 7292 7926 Size: 613 634 Fragments arranged by size: 2205 1788 1763 634 613 381 266 197 67 12 ClaI AT'CG_AT Cuts at: 0 1857 7926 Size: 1857 6069 DraI TTT'AAA Cuts at: 0 1016 1053 1073 1707 1880 2762 4767 7749 Size: 1016 37 20 634 173 882 2005 2982 Cuts at: 7749 7926 Size: 177 Fragments arranged by size: 2982 2005 1016 882 634 177 173 37 20 DrdI GACnn_nn'nnGTC Cuts at: 0 7136 7926 Size: 7136 790 EaeI y'GGCC_r Cuts at: 0 1342 5023 5598 6257 7926 Size: 1342 3681 575 659 1669 Fragments arranged by size: 3681 1669 1342 659 575 EagI C'GGCC_G Cuts at: 0 5598 7926 Size: 5598 2328 EarI CTCTTCn'nnn_ Cuts at: 0 326 1382 1437 1512 2651 2913 3126 3593 Size: 326 1056 55 75 1139 262 213 467 Cuts at: 3593 3752 4004 5621 5984 6176 6492 7926 Size: 159 252 1617 363 192 316 1434 Fragments arranged by size: 1617 1434 1139 1056 467 363 326 316 262 252 213 192 159 75 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 557 1218 7926 Size: 557 661 6708 Fragments arranged by size: 6708 661 557 EcoICRI GAG'CTC Cuts at: 0 1061 1310 3762 4528 7926 Size: 1061 249 2452 766 3398 Fragments arranged by size: 3398 2452 1061 766 249 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 89 213 293 307 962 1547 1826 2426 Size: 89 124 80 14 655 585 279 600 Cuts at: 2426 2441 2632 2699 3181 4063 4246 4344 4846 Size: 15 191 67 482 882 183 98 502 Cuts at: 4846 4886 5189 5554 5605 6357 6972 7201 7605 Size: 40 303 365 51 752 615 229 404 Cuts at: 7605 7760 7816 7843 7926 Size: 155 56 27 83 Fragments arranged by size: 882 752 655 615 600 585 502 482 404 365 303 279 229 191 183 155 124 98 89 83 80 67 56 51 40 27 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 1257 2813 6292 7926 Size: 1257 1556 3479 1634 Fragments arranged by size: 3479 1634 1556 1257 EcoO109I rG'GnC_Cy Cuts at: 0 4204 4337 4365 7818 7926 Size: 4204 133 28 3453 108 Fragments arranged by size: 4204 3453 133 108 28 EcoRI G'AATT_C Cuts at: 0 2898 3222 6305 7926 Size: 2898 324 3083 1621 Fragments arranged by size: 3083 2898 1621 324 EcoRV GAT'ATC Cuts at: 0 4191 7926 Size: 4191 3735 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4576 5974 7926 Size: 4576 1398 1952 Fragments arranged by size: 4576 1952 1398 HaeII r_GCGC'y Cuts at: 0 4965 7926 Size: 4965 2961 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 355 387 760 792 817 849 1948 1980 Size: 355 32 373 32 25 32 1099 32 Cuts at: 1980 2695 2727 2918 2950 3514 3546 3910 3942 Size: 715 32 191 32 564 32 364 32 Cuts at: 3942 4078 4110 4828 4830 4860 4862 4873 4905 Size: 136 32 718 2 30 2 11 32 Cuts at: 4905 5306 5338 5761 5793 6178 6210 7338 7370 Size: 401 32 423 32 385 32 1128 32 Cuts at: 7370 7926 Size: 556 Fragments arranged by size: 1128 1099 718 715 564 556 423 401 385 373 364 355 191 136 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 25 11 2 2 HincII GTy'rAC Cuts at: 0 5219 7926 Size: 5219 2707 HindIII A'AGCT_T Cuts at: 0 5617 7926 Size: 5617 2309 HpaI GTT'AAC Cuts at: 0 5219 7926 Size: 5219 2707 KasI G'GCGC_C Cuts at: 0 4961 7926 Size: 4961 2965 MfeI C'AATT_G Cuts at: 0 5283 7187 7926 Size: 5283 1904 739 Fragments arranged by size: 5283 1904 739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1641 5657 6259 7165 7582 7926 Size: 1641 4016 602 906 417 344 Fragments arranged by size: 4016 1641 906 602 417 344 MscI TGG'CCA Cuts at: 0 5025 6259 7926 Size: 5025 1234 1667 Fragments arranged by size: 5025 1667 1234 MslI CAynn'nnrTG Cuts at: 0 1280 2284 2600 3057 3335 3521 3737 4374 Size: 1280 1004 316 457 278 186 216 637 Cuts at: 4374 4649 5123 7926 Size: 275 474 2803 Fragments arranged by size: 2803 1280 1004 637 474 457 316 278 275 216 186 MspA1I CmG'CkG Cuts at: 0 9 313 5123 6030 7926 Size: 9 304 4810 907 1896 Fragments arranged by size: 4810 1896 907 304 9 NaeI GCC'GGC Cuts at: 0 7013 7926 Size: 7013 913 NarI GG'CG_CC Cuts at: 0 4962 7926 Size: 4962 2964 NcoI C'CATG_G Cuts at: 0 3023 3236 7926 Size: 3023 213 4690 Fragments arranged by size: 4690 3023 213 NdeI CA'TA_TG Cuts at: 0 7563 7926 Size: 7563 363 NgoMIV G'CCGG_C Cuts at: 0 7011 7926 Size: 7011 915 NsiI A_TGCA'T Cuts at: 0 2143 3054 7926 Size: 2143 911 4872 Fragments arranged by size: 4872 2143 911 NspI r_CATG'y Cuts at: 0 2141 2145 2211 2546 2770 2968 3736 4547 Size: 2141 4 66 335 224 198 768 811 Cuts at: 4547 4648 6919 7139 7878 7926 Size: 101 2271 220 739 48 Fragments arranged by size: 2271 2141 811 768 739 335 224 220 198 101 66 48 4 PciI A'CATG_T Cuts at: 0 2964 3732 6915 7135 7926 Size: 2964 768 3183 220 791 Fragments arranged by size: 3183 2964 791 768 220 PflMI CCAn_nnn'nTGG Cuts at: 0 6954 7926 Size: 6954 972 PfoI T'CCnGG_A Cuts at: 0 3939 6269 7926 Size: 3939 2330 1657 Fragments arranged by size: 3939 2330 1657 PmlI CAC'GTG Cuts at: 0 3889 7926 Size: 3889 4037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 5503 5535 7926 Size: 5503 32 2391 Fragments arranged by size: 5503 2391 32 PpuMI rG'GwC_Cy Cuts at: 0 4365 7818 7926 Size: 4365 3453 108 Fragments arranged by size: 4365 3453 108 PshAI GACnn'nnGTC Cuts at: 0 1623 5731 7926 Size: 1623 4108 2195 Fragments arranged by size: 4108 2195 1623 PsiI TTA'TAA Cuts at: 0 730 1664 1921 5635 5884 6704 7926 Size: 730 934 257 3714 249 820 1222 Fragments arranged by size: 3714 1222 934 820 730 257 249 PspOMI G'GGCC_C Cuts at: 0 4336 7926 Size: 4336 3590 PspXI vC'TCGA_Gb Cuts at: 0 4772 7926 Size: 4772 3154 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2562 2594 2733 2765 7926 Size: 2562 32 139 32 5161 Fragments arranged by size: 5161 2562 139 32 32 PstI C_TGCA'G Cuts at: 0 14 381 7926 Size: 14 367 7545 Fragments arranged by size: 7545 367 14 PvuII CAG'CTG Cuts at: 0 9 313 5123 6030 7926 Size: 9 304 4810 907 1896 Fragments arranged by size: 4810 1896 907 304 9 SacI G_AGCT'C Cuts at: 0 1063 1312 3764 4530 7926 Size: 1063 249 2452 766 3396 Fragments arranged by size: 3396 2452 1063 766 249 SanDI GG'GwC_CC Cuts at: 0 4365 7926 Size: 4365 3561 SapI GCTCTTCn'nnn_ Cuts at: 0 3126 3593 3752 7926 Size: 3126 467 159 4174 Fragments arranged by size: 4174 3126 467 159 ScaI AGT'ACT Cuts at: 0 5693 7090 7801 7926 Size: 5693 1397 711 125 Fragments arranged by size: 5693 1397 711 125 SexAI A'CCwGG_T Cuts at: 0 5432 6738 7926 Size: 5432 1306 1188 Fragments arranged by size: 5432 1306 1188 SfcI C'TryA_G Cuts at: 0 10 377 545 3095 5981 6584 6872 7926 Size: 10 367 168 2550 2886 603 288 1054 Fragments arranged by size: 2886 2550 1054 603 367 288 168 10 SfoI GGC'GCC Cuts at: 0 4963 7926 Size: 4963 2963 SmlI C'TyrA_G Cuts at: 0 1407 2885 3788 3968 4529 4689 4772 7106 Size: 1407 1478 903 180 561 160 83 2334 Cuts at: 7106 7427 7525 7926 Size: 321 98 401 Fragments arranged by size: 2334 1478 1407 903 561 401 321 180 160 98 83 SpeI A'CTAG_T Cuts at: 0 2575 7926 Size: 2575 5351 SphI G_CATG'C Cuts at: 0 2141 2145 2211 2770 4648 7926 Size: 2141 4 66 559 1878 3278 Fragments arranged by size: 3278 2141 1878 559 66 4 SspI AAT'ATT Cuts at: 0 809 1129 2169 2433 4735 7926 Size: 809 320 1040 264 2302 3191 Fragments arranged by size: 3191 2302 1040 809 320 264 StuI AGG'CCT Cuts at: 0 6757 7585 7926 Size: 6757 828 341 Fragments arranged by size: 6757 828 341 StyI C'CwwG_G Cuts at: 0 3023 3236 6857 7853 7926 Size: 3023 213 3621 996 73 Fragments arranged by size: 3621 3023 996 213 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3563 6822 7926 Size: 3563 3259 1104 Fragments arranged by size: 3563 3259 1104 TatI w'GTAC_w Cuts at: 0 1723 2383 3508 4501 5691 6975 7077 7088 Size: 1723 660 1125 993 1190 1284 102 11 Cuts at: 7088 7191 7799 7859 7871 7926 Size: 103 608 60 12 55 Fragments arranged by size: 1723 1284 1190 1125 993 660 608 103 102 60 55 12 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2657 2689 2951 2983 4371 4403 5509 5541 Size: 2657 32 262 32 1388 32 1106 32 Cuts at: 5541 5793 5825 5832 5864 6808 6840 6970 7002 Size: 252 32 7 32 944 32 130 32 Cuts at: 7002 7236 7268 7810 7842 7926 Size: 234 32 542 32 84 Fragments arranged by size: 2657 1388 1106 944 542 262 252 234 130 84 32 32 32 32 32 32 32 32 32 32 7 Tth111I GACn'n_nGTC Cuts at: 0 4842 7926 Size: 4842 3084 XbaI T'CTAG_A Cuts at: 0 1237 7926 Size: 1237 6689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 465 3644 6267 6810 7926 Size: 465 3179 2623 543 1116 Fragments arranged by size: 3179 2623 1116 543 465 XhoI C'TCGA_G Cuts at: 0 4772 7427 7926 Size: 4772 2655 499 Fragments arranged by size: 4772 2655 499 XmnI GAAnn'nnTTC Cuts at: 0 872 5266 7926 Size: 872 4394 2660 Fragments arranged by size: 4394 2660 872 Enzymes that do cut: AarI AccI AclI AcuI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApoI AseI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI ApaLI AscI AsiSI AvrII BcgI BciVI BglI BmgBI BmtI BsiWI BssHII BstZ17I CspCI DraIII FseI FspI FspAI KpnI MluI NheI NotI NruI PacI PasI PmeI PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI SmaI SnaBI SrfI SwaI XmaI ZraI