Restriction map for
pF1KA1732
(Linear) (Six-base) MAPSORT of: KIAA1732.seq Check: 9271 from: 1 to: 7926
KIAA1732.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 6396 7926
Size: 594 5802 1530
Fragments arranged by size:
5802 1530 594
AccI GT'mk_AC
Cuts at: 0 329 548 3094 3684 3933 7926
Size: 329 219 2546 590 249 3993
Fragments arranged by size:
3993 2546 590 329 249 219
AclI AA'CG_TT
Cuts at: 0 5221 7926
Size: 5221 2705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 213 307 962 1547 2426 2441 2632
Size: 89 124 94 655 585 879 15 191
Cuts at: 2632 4063 4246 4344 4846 5189 5554 5605 6357
Size: 1431 183 98 502 343 365 51 752
Cuts at: 6357 7201 7760 7816 7926
Size: 844 559 56 110
Fragments arranged by size:
1431 879 844 752 655 585 559 502
365 343 191 183 124 110 98 94
89 56 51 15
AflIII A'CryG_T
Cuts at: 0 795 2964 3732 3886 6915 7135 7926
Size: 795 2169 768 154 3029 220 791
Fragments arranged by size:
3029 2169 795 791 768 220 154
AhdI GACnn_n'nnGTC
Cuts at: 0 4092 7926
Size: 4092 3834
AleI CACnn'nnGTG
Cuts at: 0 5123 7926
Size: 5123 2803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2073 7926
Size: 2073 5853
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1598 1630 7926
Size: 1598 32 6296
Fragments arranged by size:
6296 1598 32
AlwNI CAG_nnn'CTG
Cuts at: 0 967 2041 2587 5836 6100 6663 6928 7689
Size: 967 1074 546 3249 264 563 265 761
Cuts at: 7689 7926
Size: 237
Fragments arranged by size:
3249 1074 967 761 563 546 265 264
237
ApaI G_GGCC'C
Cuts at: 0 4340 7926
Size: 4340 3586
ApoI r'AATT_y
Cuts at: 0 422 872 1018 1702 1710 1915 2517 2536
Size: 422 450 146 684 8 205 602 19
Cuts at: 2536 2898 3015 3222 3346 3425 3552 4063 6305
Size: 362 117 207 124 79 127 511 2242
Cuts at: 6305 7926
Size: 1621
Fragments arranged by size:
2242 1621 684 602 511 450 422 362
207 205 146 127 124 117 79 19
8
AseI AT'TA_AT
Cuts at: 0 1808 6155 7926
Size: 1808 4347 1771
Fragments arranged by size:
4347 1808 1771
AvaI C'yCGr_G
Cuts at: 0 4772 7427 7926
Size: 4772 2655 499
Fragments arranged by size:
4772 2655 499
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2330 2363 3838 3871 7926
Size: 2330 33 1475 33 4055
Fragments arranged by size:
4055 2330 1475 33 33
BamHI G'GATC_C
Cuts at: 0 3918 4036 6765 7485 7926
Size: 3918 118 2729 720 441
Fragments arranged by size:
3918 2729 720 441 118
BanI G'GyrC_C
Cuts at: 0 172 4961 6909 7926
Size: 172 4789 1948 1017
Fragments arranged by size:
4789 1948 1017 172
BanII G_rGCy'C
Cuts at: 0 1063 1312 3764 4340 4530 6119 7926
Size: 1063 249 2452 576 190 1589 1807
Fragments arranged by size:
2452 1807 1589 1063 576 249 190
BbeI G_GCGC'C
Cuts at: 0 4965 7926
Size: 4965 2961
BbsI GAAGACnn'nnnn_
Cuts at: 0 1405 2647 2931 3035 4561 6403 7207 7426
Size: 1405 1242 284 104 1526 1842 804 219
Cuts at: 7426 7748 7926
Size: 322 178
Fragments arranged by size:
1842 1526 1405 1242 804 322 284 219
178 104
BbvCI CC'TCA_GC
Cuts at: 0 4933 7316 7926
Size: 4933 2383 610
Fragments arranged by size:
4933 2383 610
BclI T'GATC_A
Cuts at: 0 1980 5961 6843 7463 7926
Size: 1980 3981 882 620 463
Fragments arranged by size:
3981 1980 882 620 463
BfrBI ATG'CAT
Cuts at: 0 2141 3052 7926
Size: 2141 911 4874
Fragments arranged by size:
4874 2141 911
BglII A'GATC_T
Cuts at: 0 706 1210 4881 7926
Size: 706 504 3671 3045
Fragments arranged by size:
3671 3045 706 504
BlpI GC'TnA_GC
Cuts at: 0 1062 5440 5824 6851 7926
Size: 1062 4378 384 1027 1075
Fragments arranged by size:
4378 1075 1062 1027 384
Bme1580I G_kGCm'C
Cuts at: 0 4316 4340 7926
Size: 4316 24 3586
Fragments arranged by size:
4316 3586 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1618 3697 3924 3956 6814 6942 7018 7665
Size: 1618 2079 227 32 2858 128 76 647
Cuts at: 7665 7926
Size: 261
Fragments arranged by size:
2858 2079 1618 647 261 227 128 76
32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1229 1251 4765 4866 5436 7926
Size: 1229 22 3514 101 570 2490
Fragments arranged by size:
3514 2490 1229 570 101 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1826 2699 3181 4886 6972 7605 7843
Size: 293 1533 873 482 1705 2086 633 238
Cuts at: 7843 7926
Size: 83
Fragments arranged by size:
2086 1705 1533 873 633 482 293 238
83
Bpu10I CC'TnA_GC
Cuts at: 0 4933 7316 7926
Size: 4933 2383 610
Fragments arranged by size:
4933 2383 610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1392 2870 3773 3953 4514 4710 7091 7546
Size: 1392 1478 903 180 561 196 2381 455
Cuts at: 7546 7926
Size: 380
Fragments arranged by size:
2381 1478 1392 903 561 455 380 196
180
BsaI GGTCTCn'nnnn_
Cuts at: 0 1436 1486 1844 5039 5422 5720 6416 7926
Size: 1436 50 358 3195 383 298 696 1510
Fragments arranged by size:
3195 1510 1436 696 383 358 298 50
BsaAI yAC'GTr
Cuts at: 0 798 3809 3889 7926
Size: 798 3011 80 4037
Fragments arranged by size:
4037 3011 798 80
BsaBI GATnn'nnATC
Cuts at: 0 1182 2609 5960 7926
Size: 1182 1427 3351 1966
Fragments arranged by size:
3351 1966 1427 1182
BsaHI Gr'CG_yC
Cuts at: 0 4962 7926
Size: 4962 2964
BsaWI w'CCGG_w
Cuts at: 0 3420 7700 7926
Size: 3420 4280 226
Fragments arranged by size:
4280 3420 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2616 2646 2951 2981 5832 5862 6002 6032
Size: 2616 30 305 30 2851 30 140 30
Cuts at: 6032 6178 6208 6970 7000 7253 7283 7783 7813
Size: 146 30 762 30 253 30 500 30
Cuts at: 7813 7926
Size: 113
Fragments arranged by size:
2851 2616 762 500 305 253 146 140
113 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2966 4027 5318 6199 7370 7926
Size: 2966 1061 1291 881 1171 556
Fragments arranged by size:
2966 1291 1171 1061 881 556
BseYI C'CCAG_C
Cuts at: 0 1834 4682 4949 6769 6808 7205 7926
Size: 1834 2848 267 1820 39 397 721
Fragments arranged by size:
2848 1834 1820 721 397 267 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 254 4891 7082 7926
Size: 124 130 4637 2191 844
Fragments arranged by size:
4637 2191 844 130 124
BsiEI CG_ry'CG
Cuts at: 0 5601 7926
Size: 5601 2325
BsiHKAI G_wGCw'C
Cuts at: 0 59 1063 1312 1365 3764 4530 5441 5787
Size: 59 1004 249 53 2399 766 911 346
Cuts at: 5787 5830 6797 7926
Size: 43 967 1129
Fragments arranged by size:
2399 1129 1004 967 911 766 346 249
59 53 43
BsmI GAATG_Cn'
Cuts at: 0 4493 6043 7926
Size: 4493 1550 1883
Fragments arranged by size:
4493 1883 1550
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3199 4831 4997 7926
Size: 3199 1632 166 2929
Fragments arranged by size:
3199 2929 1632 166
Bsp1286I G_dGCh'C
Cuts at: 0 59 1063 1312 1365 3764 4316 4340 4530
Size: 59 1004 249 53 2399 552 24 190
Cuts at: 4530 5441 5787 5830 6119 6797 7926
Size: 911 346 43 289 678 1129
Fragments arranged by size:
2399 1129 1004 911 678 552 346 289
249 190 59 53 43 24
BspEI T'CCGG_A
Cuts at: 0 3420 7926
Size: 3420 4506
BspHI T'CATG_A
Cuts at: 0 3102 4574 5111 7926
Size: 3102 1472 537 2815
Fragments arranged by size:
3102 2815 1472 537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 2212 6396 7926
Size: 594 1618 4184 1530
Fragments arranged by size:
4184 1618 1530 594
BsrBI CCG'CTC
Cuts at: 0 6501 7926
Size: 6501 1425
BsrDI GCAATG_nn'
Cuts at: 0 285 3912 7836 7926
Size: 285 3627 3924 90
Fragments arranged by size:
3924 3627 285 90
BsrFI r'CCGG_y
Cuts at: 0 7011 7926
Size: 7011 915
BsrGI T'GTAC_A
Cuts at: 0 1723 4501 7077 7191 7926
Size: 1723 2778 2576 114 735
Fragments arranged by size:
2778 2576 1723 735 114
BssSI C'ACGA_G
Cuts at: 0 1364 7926
Size: 1364 6562
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4902 6654 7926
Size: 4902 1752 1272
Fragments arranged by size:
4902 1752 1272
BstBI TT'CG_AA
Cuts at: 0 4714 7926
Size: 4714 3212
BstEII G'GTnAC_C
Cuts at: 0 3544 5293 6730 7926
Size: 3544 1749 1437 1196
Fragments arranged by size:
3544 1749 1437 1196
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4376 5196 5245 6270 6967 7926
Size: 4376 820 49 1025 697 959
Fragments arranged by size:
4376 1025 959 820 697 49
BstYI r'GATC_y
Cuts at: 0 706 1083 1210 2023 3918 4036 4881 5573
Size: 706 377 127 813 1895 118 845 692
Cuts at: 5573 5955 6765 7362 7431 7485 7926
Size: 382 810 597 69 54 441
Fragments arranged by size:
1895 845 813 810 706 692 597 441
382 377 127 118 69 54
Bsu36I CC'TnA_GG
Cuts at: 0 1447 7814 7926
Size: 1447 6367 112
Fragments arranged by size:
6367 1447 112
BtgI C'CryG_G
Cuts at: 0 3023 3236 7926
Size: 3023 213 4690
Fragments arranged by size:
4690 3023 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4389 5762 7926
Size: 4389 1373 2164
Fragments arranged by size:
4389 2164 1373
BtsI GCAGTG_nn'
Cuts at: 0 266 463 530 2735 4498 4879 4891 6679
Size: 266 197 67 2205 1763 381 12 1788
Cuts at: 6679 7292 7926
Size: 613 634
Fragments arranged by size:
2205 1788 1763 634 613 381 266 197
67 12
ClaI AT'CG_AT
Cuts at: 0 1857 7926
Size: 1857 6069
DraI TTT'AAA
Cuts at: 0 1016 1053 1073 1707 1880 2762 4767 7749
Size: 1016 37 20 634 173 882 2005 2982
Cuts at: 7749 7926
Size: 177
Fragments arranged by size:
2982 2005 1016 882 634 177 173 37
20
DrdI GACnn_nn'nnGTC
Cuts at: 0 7136 7926
Size: 7136 790
EaeI y'GGCC_r
Cuts at: 0 1342 5023 5598 6257 7926
Size: 1342 3681 575 659 1669
Fragments arranged by size:
3681 1669 1342 659 575
EagI C'GGCC_G
Cuts at: 0 5598 7926
Size: 5598 2328
EarI CTCTTCn'nnn_
Cuts at: 0 326 1382 1437 1512 2651 2913 3126 3593
Size: 326 1056 55 75 1139 262 213 467
Cuts at: 3593 3752 4004 5621 5984 6176 6492 7926
Size: 159 252 1617 363 192 316 1434
Fragments arranged by size:
1617 1434 1139 1056 467 363 326 316
262 252 213 192 159 75 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 557 1218 7926
Size: 557 661 6708
Fragments arranged by size:
6708 661 557
EcoICRI GAG'CTC
Cuts at: 0 1061 1310 3762 4528 7926
Size: 1061 249 2452 766 3398
Fragments arranged by size:
3398 2452 1061 766 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 213 293 307 962 1547 1826 2426
Size: 89 124 80 14 655 585 279 600
Cuts at: 2426 2441 2632 2699 3181 4063 4246 4344 4846
Size: 15 191 67 482 882 183 98 502
Cuts at: 4846 4886 5189 5554 5605 6357 6972 7201 7605
Size: 40 303 365 51 752 615 229 404
Cuts at: 7605 7760 7816 7843 7926
Size: 155 56 27 83
Fragments arranged by size:
882 752 655 615 600 585 502 482
404 365 303 279 229 191 183 155
124 98 89 83 80 67 56 51
40 27 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1257 2813 6292 7926
Size: 1257 1556 3479 1634
Fragments arranged by size:
3479 1634 1556 1257
EcoO109I rG'GnC_Cy
Cuts at: 0 4204 4337 4365 7818 7926
Size: 4204 133 28 3453 108
Fragments arranged by size:
4204 3453 133 108 28
EcoRI G'AATT_C
Cuts at: 0 2898 3222 6305 7926
Size: 2898 324 3083 1621
Fragments arranged by size:
3083 2898 1621 324
EcoRV GAT'ATC
Cuts at: 0 4191 7926
Size: 4191 3735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4576 5974 7926
Size: 4576 1398 1952
Fragments arranged by size:
4576 1952 1398
HaeII r_GCGC'y
Cuts at: 0 4965 7926
Size: 4965 2961
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 760 792 817 849 1948 1980
Size: 355 32 373 32 25 32 1099 32
Cuts at: 1980 2695 2727 2918 2950 3514 3546 3910 3942
Size: 715 32 191 32 564 32 364 32
Cuts at: 3942 4078 4110 4828 4830 4860 4862 4873 4905
Size: 136 32 718 2 30 2 11 32
Cuts at: 4905 5306 5338 5761 5793 6178 6210 7338 7370
Size: 401 32 423 32 385 32 1128 32
Cuts at: 7370 7926
Size: 556
Fragments arranged by size:
1128 1099 718 715 564 556 423 401
385 373 364 355 191 136 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 25 11 2
2
HincII GTy'rAC
Cuts at: 0 5219 7926
Size: 5219 2707
HindIII A'AGCT_T
Cuts at: 0 5617 7926
Size: 5617 2309
HpaI GTT'AAC
Cuts at: 0 5219 7926
Size: 5219 2707
KasI G'GCGC_C
Cuts at: 0 4961 7926
Size: 4961 2965
MfeI C'AATT_G
Cuts at: 0 5283 7187 7926
Size: 5283 1904 739
Fragments arranged by size:
5283 1904 739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1641 5657 6259 7165 7582 7926
Size: 1641 4016 602 906 417 344
Fragments arranged by size:
4016 1641 906 602 417 344
MscI TGG'CCA
Cuts at: 0 5025 6259 7926
Size: 5025 1234 1667
Fragments arranged by size:
5025 1667 1234
MslI CAynn'nnrTG
Cuts at: 0 1280 2284 2600 3057 3335 3521 3737 4374
Size: 1280 1004 316 457 278 186 216 637
Cuts at: 4374 4649 5123 7926
Size: 275 474 2803
Fragments arranged by size:
2803 1280 1004 637 474 457 316 278
275 216 186
MspA1I CmG'CkG
Cuts at: 0 9 313 5123 6030 7926
Size: 9 304 4810 907 1896
Fragments arranged by size:
4810 1896 907 304 9
NaeI GCC'GGC
Cuts at: 0 7013 7926
Size: 7013 913
NarI GG'CG_CC
Cuts at: 0 4962 7926
Size: 4962 2964
NcoI C'CATG_G
Cuts at: 0 3023 3236 7926
Size: 3023 213 4690
Fragments arranged by size:
4690 3023 213
NdeI CA'TA_TG
Cuts at: 0 7563 7926
Size: 7563 363
NgoMIV G'CCGG_C
Cuts at: 0 7011 7926
Size: 7011 915
NsiI A_TGCA'T
Cuts at: 0 2143 3054 7926
Size: 2143 911 4872
Fragments arranged by size:
4872 2143 911
NspI r_CATG'y
Cuts at: 0 2141 2145 2211 2546 2770 2968 3736 4547
Size: 2141 4 66 335 224 198 768 811
Cuts at: 4547 4648 6919 7139 7878 7926
Size: 101 2271 220 739 48
Fragments arranged by size:
2271 2141 811 768 739 335 224 220
198 101 66 48 4
PciI A'CATG_T
Cuts at: 0 2964 3732 6915 7135 7926
Size: 2964 768 3183 220 791
Fragments arranged by size:
3183 2964 791 768 220
PflMI CCAn_nnn'nTGG
Cuts at: 0 6954 7926
Size: 6954 972
PfoI T'CCnGG_A
Cuts at: 0 3939 6269 7926
Size: 3939 2330 1657
Fragments arranged by size:
3939 2330 1657
PmlI CAC'GTG
Cuts at: 0 3889 7926
Size: 3889 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5503 5535 7926
Size: 5503 32 2391
Fragments arranged by size:
5503 2391 32
PpuMI rG'GwC_Cy
Cuts at: 0 4365 7818 7926
Size: 4365 3453 108
Fragments arranged by size:
4365 3453 108
PshAI GACnn'nnGTC
Cuts at: 0 1623 5731 7926
Size: 1623 4108 2195
Fragments arranged by size:
4108 2195 1623
PsiI TTA'TAA
Cuts at: 0 730 1664 1921 5635 5884 6704 7926
Size: 730 934 257 3714 249 820 1222
Fragments arranged by size:
3714 1222 934 820 730 257 249
PspOMI G'GGCC_C
Cuts at: 0 4336 7926
Size: 4336 3590
PspXI vC'TCGA_Gb
Cuts at: 0 4772 7926
Size: 4772 3154
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2562 2594 2733 2765 7926
Size: 2562 32 139 32 5161
Fragments arranged by size:
5161 2562 139 32 32
PstI C_TGCA'G
Cuts at: 0 14 381 7926
Size: 14 367 7545
Fragments arranged by size:
7545 367 14
PvuII CAG'CTG
Cuts at: 0 9 313 5123 6030 7926
Size: 9 304 4810 907 1896
Fragments arranged by size:
4810 1896 907 304 9
SacI G_AGCT'C
Cuts at: 0 1063 1312 3764 4530 7926
Size: 1063 249 2452 766 3396
Fragments arranged by size:
3396 2452 1063 766 249
SanDI GG'GwC_CC
Cuts at: 0 4365 7926
Size: 4365 3561
SapI GCTCTTCn'nnn_
Cuts at: 0 3126 3593 3752 7926
Size: 3126 467 159 4174
Fragments arranged by size:
4174 3126 467 159
ScaI AGT'ACT
Cuts at: 0 5693 7090 7801 7926
Size: 5693 1397 711 125
Fragments arranged by size:
5693 1397 711 125
SexAI A'CCwGG_T
Cuts at: 0 5432 6738 7926
Size: 5432 1306 1188
Fragments arranged by size:
5432 1306 1188
SfcI C'TryA_G
Cuts at: 0 10 377 545 3095 5981 6584 6872 7926
Size: 10 367 168 2550 2886 603 288 1054
Fragments arranged by size:
2886 2550 1054 603 367 288 168 10
SfoI GGC'GCC
Cuts at: 0 4963 7926
Size: 4963 2963
SmlI C'TyrA_G
Cuts at: 0 1407 2885 3788 3968 4529 4689 4772 7106
Size: 1407 1478 903 180 561 160 83 2334
Cuts at: 7106 7427 7525 7926
Size: 321 98 401
Fragments arranged by size:
2334 1478 1407 903 561 401 321 180
160 98 83
SpeI A'CTAG_T
Cuts at: 0 2575 7926
Size: 2575 5351
SphI G_CATG'C
Cuts at: 0 2141 2145 2211 2770 4648 7926
Size: 2141 4 66 559 1878 3278
Fragments arranged by size:
3278 2141 1878 559 66 4
SspI AAT'ATT
Cuts at: 0 809 1129 2169 2433 4735 7926
Size: 809 320 1040 264 2302 3191
Fragments arranged by size:
3191 2302 1040 809 320 264
StuI AGG'CCT
Cuts at: 0 6757 7585 7926
Size: 6757 828 341
Fragments arranged by size:
6757 828 341
StyI C'CwwG_G
Cuts at: 0 3023 3236 6857 7853 7926
Size: 3023 213 3621 996 73
Fragments arranged by size:
3621 3023 996 213 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3563 6822 7926
Size: 3563 3259 1104
Fragments arranged by size:
3563 3259 1104
TatI w'GTAC_w
Cuts at: 0 1723 2383 3508 4501 5691 6975 7077 7088
Size: 1723 660 1125 993 1190 1284 102 11
Cuts at: 7088 7191 7799 7859 7871 7926
Size: 103 608 60 12 55
Fragments arranged by size:
1723 1284 1190 1125 993 660 608 103
102 60 55 12 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2657 2689 2951 2983 4371 4403 5509 5541
Size: 2657 32 262 32 1388 32 1106 32
Cuts at: 5541 5793 5825 5832 5864 6808 6840 6970 7002
Size: 252 32 7 32 944 32 130 32
Cuts at: 7002 7236 7268 7810 7842 7926
Size: 234 32 542 32 84
Fragments arranged by size:
2657 1388 1106 944 542 262 252 234
130 84 32 32 32 32 32 32
32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 0 4842 7926
Size: 4842 3084
XbaI T'CTAG_A
Cuts at: 0 1237 7926
Size: 1237 6689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 3644 6267 6810 7926
Size: 465 3179 2623 543 1116
Fragments arranged by size:
3179 2623 1116 543 465
XhoI C'TCGA_G
Cuts at: 0 4772 7427 7926
Size: 4772 2655 499
Fragments arranged by size:
4772 2655 499
XmnI GAAnn'nnTTC
Cuts at: 0 872 5266 7926
Size: 872 4394 2660
Fragments arranged by size:
4394 2660 872
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AseI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuII SacI SanDI SapI ScaI SexAI
SfcI SfoI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI ApaLI AscI AsiSI
AvrII BcgI BciVI BglI BmgBI BmtI BsiWI BssHII
BstZ17I CspCI DraIII FseI FspI FspAI KpnI MluI
NheI NotI NruI PacI PasI PmeI PvuI RsrII
SacII SalI SbfI SfiI SgfI SgrAI SmaI SnaBI
SrfI SwaI XmaI ZraI