Restriction map for
pF1KSDA1721
(Linear) (Six-base) MAPSORT of: KIAA1721.seq Check: 1157 from: 1 to: 3453
KIAA1721.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 1193 1476 3453
Size: 1193 283 1977
Fragments arranged by size:
1977 1193 283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 1037 1742 2311 2320 3453
Size: 644 393 705 569 9 1133
Fragments arranged by size:
1133 705 644 569 393 9
AflII C'TTAA_G
Cuts at: 0 2349 3160 3453
Size: 2349 811 293
Fragments arranged by size:
2349 811 293
AflIII A'CryG_T
Cuts at: 0 3236 3453
Size: 3236 217
AleI CACnn'nnGTG
Cuts at: 0 2128 3241 3453
Size: 2128 1113 212
Fragments arranged by size:
2128 1113 212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 911 2111 3453
Size: 911 1200 1342
Fragments arranged by size:
1342 1200 911
AlwNI CAG_nnn'CTG
Cuts at: 0 1807 2551 3453
Size: 1807 744 902
Fragments arranged by size:
1807 902 744
ApaI G_GGCC'C
Cuts at: 0 23 3453
Size: 23 3430
ApoI r'AATT_y
Cuts at: 0 496 541 723 1344 1748 2181 2773 3415
Size: 496 45 182 621 404 433 592 642
Cuts at: 3415 3453
Size: 38
Fragments arranged by size:
642 621 592 496 433 404 182 45
38
AseI AT'TA_AT
Cuts at: 0 1733 2947 3453
Size: 1733 1214 506
Fragments arranged by size:
1733 1214 506
AvaI C'yCGr_G
Cuts at: 0 1856 3453
Size: 1856 1597
BanI G'GyrC_C
Cuts at: 0 69 3453
Size: 69 3384
BanII G_rGCy'C
Cuts at: 0 23 3453
Size: 23 3430
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 650 3453
Size: 584 66 2803
Fragments arranged by size:
2803 584 66
BbvCI CC'TCA_GC
Cuts at: 0 2447 3453
Size: 2447 1006
BclI T'GATC_A
Cuts at: 0 1425 1971 3453
Size: 1425 546 1482
Fragments arranged by size:
1482 1425 546
BglII A'GATC_T
Cuts at: 0 590 1872 2839 3453
Size: 590 1282 967 614
Fragments arranged by size:
1282 967 614 590
BlpI GC'TnA_GC
Cuts at: 0 38 2189 3453
Size: 38 2151 1264
Fragments arranged by size:
2151 1264 38
Bme1580I G_kGCm'C
Cuts at: 0 23 3453
Size: 23 3430
BmrI ACTGGGnnnn_n'
Cuts at: 0 894 3301 3453
Size: 894 2407 152
Fragments arranged by size:
2407 894 152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1891 3453
Size: 1891 1562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1017 1313 1670 1745 2097 3097 3453
Size: 64 953 296 357 75 352 1000 356
Fragments arranged by size:
1000 953 357 356 352 296 75 64
Bpu10I CC'TnA_GC
Cuts at: 0 2447 3453
Size: 2447 1006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 3453
Size: 720 2733
BsaI GGTCTCn'nnnn_
Cuts at: 0 1406 3442 3453
Size: 1406 2036 11
Fragments arranged by size:
2036 1406 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1213 1243 3453
Size: 1213 30 2210
Fragments arranged by size:
2210 1213 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1095 1390 3353 3453
Size: 1095 295 1963 100
Fragments arranged by size:
1963 1095 295 100
BseYI C'CCAG_C
Cuts at: 0 15 1931 3453
Size: 15 1916 1522
Fragments arranged by size:
1916 1522 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2131 2260 3453
Size: 2131 129 1193
Fragments arranged by size:
2131 1193 129
BsiHKAI G_wGCw'C
Cuts at: 0 116 3453
Size: 116 3337
BsmI GAATG_Cn'
Cuts at: 0 1451 1713 3453
Size: 1451 262 1740
Fragments arranged by size:
1740 1451 262
Bsp1286I G_dGCh'C
Cuts at: 0 23 116 3453
Size: 23 93 3337
Fragments arranged by size:
3337 93 23
BspHI T'CATG_A
Cuts at: 0 414 3453
Size: 414 3039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2738 2768 3453
Size: 2738 30 685
Fragments arranged by size:
2738 685 30
BsrDI GCAATG_nn'
Cuts at: 0 2441 3453
Size: 2441 1012
BssSI C'ACGA_G
Cuts at: 0 1370 3453
Size: 1370 2083
BstAPI GCAn_nnn'nTGC
Cuts at: 0 768 3453
Size: 768 2685
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1362 3453
Size: 1362 2091
BstYI r'GATC_y
Cuts at: 0 590 1028 1756 1872 2839 2904 3000 3453
Size: 590 438 728 116 967 65 96 453
Fragments arranged by size:
967 728 590 453 438 116 96 65
BstZ17I GTA'TAC
Cuts at: 0 1194 1477 3453
Size: 1194 283 1976
Fragments arranged by size:
1976 1194 283
Bsu36I CC'TnA_GG
Cuts at: 0 2996 3453
Size: 2996 457
BtgI C'CryG_G
Cuts at: 0 80 546 1521 3453
Size: 80 466 975 1932
Fragments arranged by size:
1932 975 466 80
BtsI GCAGTG_nn'
Cuts at: 0 895 2414 3212 3347 3453
Size: 895 1519 798 135 106
Fragments arranged by size:
1519 895 798 135 106
DraI TTT'AAA
Cuts at: 0 1925 3009 3453
Size: 1925 1084 444
Fragments arranged by size:
1925 1084 444
DrdI GACnn_nn'nnGTC
Cuts at: 0 1523 3453
Size: 1523 1930
EaeI y'GGCC_r
Cuts at: 0 1433 3453
Size: 1433 2020
EarI CTCTTCn'nnn_
Cuts at: 0 809 917 1487 2133 2708 3453
Size: 809 108 570 646 575 745
Fragments arranged by size:
809 745 646 575 570 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 644 1017 1037 1313 1670 1742 1745
Size: 64 580 373 20 276 357 72 3
Cuts at: 1745 2097 2311 2320 3097 3453
Size: 352 214 9 777 356
Fragments arranged by size:
777 580 373 357 356 352 276 214
72 64 20 9 3
EcoO109I rG'GnC_Cy
Cuts at: 0 19 20 2091 2430 3453
Size: 19 1 2071 339 1023
Fragments arranged by size:
2071 1023 339 19 1
EcoRI G'AATT_C
Cuts at: 0 541 2773 3453
Size: 541 2232 680
Fragments arranged by size:
2232 680 541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 598 1189 2662 2749 3411 3453
Size: 598 591 1473 87 662 42
Fragments arranged by size:
1473 662 598 591 87 42
HaeII r_GCGC'y
Cuts at: 0 16 3453
Size: 16 3437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 925 957 2244 2276 2541 2573 2589 2621
Size: 925 32 1287 32 265 32 16 32
Cuts at: 2621 3453
Size: 832
Fragments arranged by size:
1287 925 832 265 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 602 1116 1232 1581 1728 3453
Size: 602 514 116 349 147 1725
Fragments arranged by size:
1725 602 514 349 147 116
HindIII A'AGCT_T
Cuts at: 0 3227 3453
Size: 3227 226
HpaI GTT'AAC
Cuts at: 0 602 1116 1232 3453
Size: 602 514 116 2221
Fragments arranged by size:
2221 602 514 116
MfeI C'AATT_G
Cuts at: 0 392 2508 3453
Size: 392 2116 945
Fragments arranged by size:
2116 945 392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 2944 3453
Size: 1199 1745 509
Fragments arranged by size:
1745 1199 509
MscI TGG'CCA
Cuts at: 0 1435 3453
Size: 1435 2018
MslI CAynn'nnrTG
Cuts at: 0 2128 3205 3241 3453
Size: 2128 1077 36 212
Fragments arranged by size:
2128 1077 212 36
MspA1I CmG'CkG
Cuts at: 0 42 2854 3453
Size: 42 2812 599
Fragments arranged by size:
2812 599 42
NcoI C'CATG_G
Cuts at: 0 80 546 1521 3453
Size: 80 466 975 1932
Fragments arranged by size:
1932 975 466 80
NdeI CA'TA_TG
Cuts at: 0 1206 2285 3453
Size: 1206 1079 1168
Fragments arranged by size:
1206 1168 1079
NspI r_CATG'y
Cuts at: 0 109 1285 3453
Size: 109 1176 2168
Fragments arranged by size:
2168 1176 109
PasI CC'CwG_GG
Cuts at: 0 1786 3453
Size: 1786 1667
PfoI T'CCnGG_A
Cuts at: 0 1761 3453
Size: 1761 1692
PpuMI rG'GwC_Cy
Cuts at: 0 2091 3453
Size: 2091 1362
PshAI GACnn'nnGTC
Cuts at: 0 584 3453
Size: 584 2869
PspOMI G'GGCC_C
Cuts at: 0 19 3453
Size: 19 3434
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 329 361 1460 1492 3453
Size: 329 32 1099 32 1961
Fragments arranged by size:
1961 1099 329 32 32
PstI C_TGCA'G
Cuts at: 0 620 890 1467 3453
Size: 620 270 577 1986
Fragments arranged by size:
1986 620 577 270
PvuII CAG'CTG
Cuts at: 0 42 3453
Size: 42 3411
SapI GCTCTTCn'nnn_
Cuts at: 0 2708 3453
Size: 2708 745
ScaI AGT'ACT
Cuts at: 0 2938 3453
Size: 2938 515
SfcI C'TryA_G
Cuts at: 0 616 886 1463 3453
Size: 616 270 577 1990
Fragments arranged by size:
1990 616 577 270
SmlI C'TyrA_G
Cuts at: 0 699 1856 2349 3160 3453
Size: 699 1157 493 811 293
Fragments arranged by size:
1157 811 699 493 293
SpeI A'CTAG_T
Cuts at: 0 178 3453
Size: 178 3275
SphI G_CATG'C
Cuts at: 0 109 3453
Size: 109 3344
SspI AAT'ATT
Cuts at: 0 1702 3265 3453
Size: 1702 1563 188
Fragments arranged by size:
1702 1563 188
StuI AGG'CCT
Cuts at: 0 3082 3453
Size: 3082 371
StyI C'CwwG_G
Cuts at: 0 80 546 1521 3453
Size: 80 466 975 1932
Fragments arranged by size:
1932 975 466 80
TatI w'GTAC_w
Cuts at: 0 840 1993 2936 3020 3453
Size: 840 1153 943 84 433
Fragments arranged by size:
1153 943 840 433 84
Tth111I GACn'n_nGTC
Cuts at: 0 193 3453
Size: 193 3260
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 788 3453
Size: 219 569 2665
Fragments arranged by size:
2665 569 219
XhoI C'TCGA_G
Cuts at: 0 1856 3453
Size: 1856 1597
XmnI GAAnn'nnTTC
Cuts at: 0 341 581 1613 1803 3453
Size: 341 240 1032 190 1650
Fragments arranged by size:
1650 1032 341 240 190
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI BbvCI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraI DrdI EaeI EarI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI
PasI PfoI PpuMI PshAI PspOMI PsrI PstI PvuII
SapI ScaI SfcI SmlI SpeI SphI SspI StuI
StyI TatI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BfrBI BglI BmgBI BmtI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BtgZI ClaI CspCI DraIII EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
SwaI TaqII TstI XbaI XmaI ZraI