Restriction map for
pF1KA1717
(Linear) (Six-base) MAPSORT of: KIAA1717.seq Check: 5358 from: 1 to: 1098
KIAA1717.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 260 586 1098
Size: 260 326 512
Fragments arranged by size:
512 326 260
Acc65I G'GTAC_C
Cuts at: 0 1055 1098
Size: 1055 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1061 1090 1098
Size: 545 516 29 8
Fragments arranged by size:
545 516 29 8
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 784 816 1098
Size: 784 32 282
Fragments arranged by size:
784 282 32
AlwNI CAG_nnn'CTG
Cuts at: 0 776 1098
Size: 776 322
ApaI G_GGCC'C
Cuts at: 0 783 944 1098
Size: 783 161 154
Fragments arranged by size:
783 161 154
ApoI r'AATT_y
Cuts at: 0 472 561 1098
Size: 472 89 537
Fragments arranged by size:
537 472 89
AvaI C'yCGr_G
Cuts at: 0 1024 1098
Size: 1024 74
BanI G'GyrC_C
Cuts at: 0 41 1055 1098
Size: 41 1014 43
Fragments arranged by size:
1014 43 41
BanII G_rGCy'C
Cuts at: 0 783 839 944 992 1098
Size: 783 56 105 48 106
Fragments arranged by size:
783 106 105 56 48
BciVI GTATCCnnnnn_n'
Cuts at: 0 861 1098
Size: 861 237
BfrBI ATG'CAT
Cuts at: 0 953 1098
Size: 953 145
BglI GCCn_nnn'nGGC
Cuts at: 0 1081 1098
Size: 1081 17
Bme1580I G_kGCm'C
Cuts at: 0 44 253 783 944 1098
Size: 44 209 530 161 154
Fragments arranged by size:
530 209 161 154 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 786 1098
Size: 786 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 229 440 698 1098
Size: 196 33 211 258 400
Fragments arranged by size:
400 258 211 196 33
Bpu10I CC'TnA_GC
Cuts at: 0 833 1098
Size: 833 265
BsaAI yAC'GTr
Cuts at: 0 254 849 1098
Size: 254 595 249
Fragments arranged by size:
595 254 249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 784 814 850 880 1098
Size: 784 30 36 30 218
Fragments arranged by size:
784 218 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 81 1098
Size: 40 41 1017
Fragments arranged by size:
1017 41 40
BseYI C'CCAG_C
Cuts at: 0 1088 1098
Size: 1088 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1098
Size: 60 1038
BsiHKAI G_wGCw'C
Cuts at: 0 992 1098
Size: 992 106
Bsp1286I G_dGCh'C
Cuts at: 0 44 253 783 839 944 992 1098
Size: 44 209 530 56 105 48 106
Fragments arranged by size:
530 209 106 105 56 48 44
BstBI TT'CG_AA
Cuts at: 0 744 1098
Size: 744 354
BstYI r'GATC_y
Cuts at: 0 624 1098
Size: 624 474
BtsI GCAGTG_nn'
Cuts at: 0 977 1098
Size: 977 121
EarI CTCTTCn'nnn_
Cuts at: 0 620 980 1024 1098
Size: 620 360 44 74
Fragments arranged by size:
620 360 74 44
EcoICRI GAG'CTC
Cuts at: 0 990 1098
Size: 990 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 229 440 545 698 1061 1090 1098
Size: 196 33 211 105 153 363 29 8
Fragments arranged by size:
363 211 196 153 105 33 29 8
EcoO109I rG'GnC_Cy
Cuts at: 0 709 780 1098
Size: 709 71 318
Fragments arranged by size:
709 318 71
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 171 1098
Size: 171 927
HincII GTy'rAC
Cuts at: 0 587 765 1098
Size: 587 178 333
Fragments arranged by size:
587 333 178
KpnI G_GTAC'C
Cuts at: 0 1059 1098
Size: 1059 39
MslI CAynn'nnrTG
Cuts at: 0 826 1098
Size: 826 272
NsiI A_TGCA'T
Cuts at: 0 955 1098
Size: 955 143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 1098
Size: 784 32 282
Fragments arranged by size:
784 282 32
PpuMI rG'GwC_Cy
Cuts at: 0 709 1098
Size: 709 389
PsiI TTA'TAA
Cuts at: 0 734 1098
Size: 734 364
PspOMI G'GGCC_C
Cuts at: 0 779 940 1098
Size: 779 161 158
Fragments arranged by size:
779 161 158
SacI G_AGCT'C
Cuts at: 0 992 1098
Size: 992 106
SalI G'TCGA_C
Cuts at: 0 585 1098
Size: 585 513
SapI GCTCTTCn'nnn_
Cuts at: 0 620 980 1098
Size: 620 360 118
Fragments arranged by size:
620 360 118
ScaI AGT'ACT
Cuts at: 0 859 1098
Size: 859 239
SexAI A'CCwGG_T
Cuts at: 0 1058 1098
Size: 1058 40
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1081 1098
Size: 1081 17
SmaI CCC'GGG
Cuts at: 0 1026 1098
Size: 1026 72
StuI AGG'CCT
Cuts at: 0 537 1075 1098
Size: 537 538 23
Fragments arranged by size:
538 537 23
StyI C'CwwG_G
Cuts at: 0 869 1098
Size: 869 229
TatI w'GTAC_w
Cuts at: 0 857 1098
Size: 857 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 551 583 1098
Size: 551 32 515
Fragments arranged by size:
551 515 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 934 1098
Size: 934 164
XmaI C'CCGG_G
Cuts at: 0 1024 1098
Size: 1024 74
XmnI GAAnn'nnTTC
Cuts at: 0 151 1075 1098
Size: 151 924 23
Fragments arranged by size:
924 151 23
Enzymes that do cut:
AccI Acc65I AcuI AloI AlwNI ApaI ApoI AvaI
BanI BanII BciVI BfrBI BglI Bme1580I BmrI BpmI
Bpu10I BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BstBI BstYI BtsI EarI EcoICRI Eco57MI EcoO109I FalI
HincII KpnI MslI NsiI PpiI PpuMI PsiI PspOMI
SacI SalI SapI ScaI SexAI SfiI SmaI StuI
StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BclI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PspXI PsrI PstI
PvuI PvuII RsrII SacII SanDI SbfI SfcI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XhoI ZraI