Restriction map for
pF1KSDA1715
(Linear) (Six-base) MAPSORT of: KIAA1715.seq Check: 6317 from: 1 to: 1284
KIAA1715.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 966 1284
Size: 966 318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1284
Size: 674 610
AlwNI CAG_nnn'CTG
Cuts at: 0 697 1228 1284
Size: 697 531 56
Fragments arranged by size:
697 531 56
ApaI G_GGCC'C
Cuts at: 0 559 1284
Size: 559 725
ApoI r'AATT_y
Cuts at: 0 71 88 205 868 1284
Size: 71 17 117 663 416
Fragments arranged by size:
663 416 117 71 17
AseI AT'TA_AT
Cuts at: 0 136 400 1284
Size: 136 264 884
Fragments arranged by size:
884 264 136
AvaI C'yCGr_G
Cuts at: 0 749 1284
Size: 749 535
BamHI G'GATC_C
Cuts at: 0 676 1284
Size: 676 608
BanII G_rGCy'C
Cuts at: 0 559 1284
Size: 559 725
BbsI GAAGACnn'nnnn_
Cuts at: 0 700 1284
Size: 700 584
BclI T'GATC_A
Cuts at: 0 254 1284
Size: 254 1030
Bme1580I G_kGCm'C
Cuts at: 0 559 1284
Size: 559 725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1215 1284
Size: 1215 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 482 623 701 1250 1284
Size: 282 200 141 78 549 34
Fragments arranged by size:
549 282 200 141 78 34
BsaWI w'CCGG_w
Cuts at: 0 420 1284
Size: 420 864
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 455 485 621 651 728 758 1284
Size: 455 30 136 30 77 30 526
Fragments arranged by size:
526 455 136 77 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 551 1183 1284
Size: 551 632 101
Fragments arranged by size:
632 551 101
Bsp1286I G_dGCh'C
Cuts at: 0 559 1284
Size: 559 725
BspEI T'CCGG_A
Cuts at: 0 420 1284
Size: 420 864
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 966 1284
Size: 966 318
BsrDI GCAATG_nn'
Cuts at: 0 878 1284
Size: 878 406
BstAPI GCAn_nnn'nTGC
Cuts at: 0 220 1284
Size: 220 1064
BstYI r'GATC_y
Cuts at: 0 676 1284
Size: 676 608
Bsu36I CC'TnA_GG
Cuts at: 0 935 1284
Size: 935 349
BtsI GCAGTG_nn'
Cuts at: 0 838 1284
Size: 838 446
DraI TTT'AAA
Cuts at: 0 335 1284
Size: 335 949
EarI CTCTTCn'nnn_
Cuts at: 0 964 1284
Size: 964 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 482 623 674 701 1250 1284
Size: 282 200 141 51 27 549 34
Fragments arranged by size:
549 282 200 141 51 34 27
EcoO109I rG'GnC_Cy
Cuts at: 0 555 556 1284
Size: 555 1 728
Fragments arranged by size:
728 555 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 409 439 441 471 621 653
Size: 322 32 55 30 2 30 150 32
Cuts at: 653 728 760 1284
Size: 75 32 524
Fragments arranged by size:
524 322 150 75 55 32 32 32
30 30 2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 556 1284
Size: 108 448 728
Fragments arranged by size:
728 448 108
MslI CAynn'nnrTG
Cuts at: 0 822 1284
Size: 822 462
MspA1I CmG'CkG
Cuts at: 0 469 1284
Size: 469 815
NspI r_CATG'y
Cuts at: 0 173 1284
Size: 173 1111
PflMI CCAn_nnn'nTGG
Cuts at: 0 460 1284
Size: 460 824
PfoI T'CCnGG_A
Cuts at: 0 582 1227 1284
Size: 582 645 57
Fragments arranged by size:
645 582 57
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 498 530 1284
Size: 498 32 754
Fragments arranged by size:
754 498 32
PspOMI G'GGCC_C
Cuts at: 0 555 1284
Size: 555 729
PstI C_TGCA'G
Cuts at: 0 513 1284
Size: 513 771
PvuII CAG'CTG
Cuts at: 0 469 1284
Size: 469 815
SfcI C'TryA_G
Cuts at: 0 41 509 1115 1284
Size: 41 468 606 169
Fragments arranged by size:
606 468 169 41
SspI AAT'ATT
Cuts at: 0 784 1284
Size: 784 500
XbaI T'CTAG_A
Cuts at: 0 51 1284
Size: 51 1233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 257 1284
Size: 257 1027
XmnI GAAnn'nnTTC
Cuts at: 0 525 1284
Size: 525 759
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AseI AvaI BamHI
BanII BbsI BclI Bme1580I BplI BpmI BsaWI BsaXI
BseRI Bsp1286I BspEI BspMI BsrDI BstAPI BstYI Bsu36I
BtsI DraI EarI Eco57MI EcoO109I Hin4I MmeI MslI
MspA1I NspI PflMI PfoI PpiI PspOMI PstI PvuII
SfcI SspI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII TatI TstI Tth111I XhoI XmaI ZraI