Restriction map for
pF1KA1714
(Linear) (Six-base) MAPSORT of: KIAA1714.seq Check: 6023 from: 1 to: 3381
KIAA1714.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1321 2282 3381
Size: 1321 961 1099
Fragments arranged by size:
1321 1099 961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 3381
Size: 114 3267
AfeI AGC'GCT
Cuts at: 0 2023 3381
Size: 2023 1358
AflII C'TTAA_G
Cuts at: 0 1267 3381
Size: 1267 2114
AflIII A'CryG_T
Cuts at: 0 2447 3381
Size: 2447 934
AhdI GACnn_n'nnGTC
Cuts at: 0 1173 3381
Size: 1173 2208
AleI CACnn'nnGTG
Cuts at: 0 2545 3381
Size: 2545 836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2898 3381
Size: 2898 483
AlwNI CAG_nnn'CTG
Cuts at: 0 1336 1431 1664 2037 3381
Size: 1336 95 233 373 1344
Fragments arranged by size:
1344 1336 373 233 95
ApaLI G'TGCA_C
Cuts at: 0 424 3381
Size: 424 2957
ApoI r'AATT_y
Cuts at: 0 631 656 941 2376 3381
Size: 631 25 285 1435 1005
Fragments arranged by size:
1435 1005 631 285 25
AseI AT'TA_AT
Cuts at: 0 706 3381
Size: 706 2675
AvaI C'yCGr_G
Cuts at: 0 165 3381
Size: 165 3216
AvrII C'CTAG_G
Cuts at: 0 118 490 3381
Size: 118 372 2891
Fragments arranged by size:
2891 372 118
BamHI G'GATC_C
Cuts at: 0 1572 3381
Size: 1572 1809
BanI G'GyrC_C
Cuts at: 0 1933 1946 2063 2627 3381
Size: 1933 13 117 564 754
Fragments arranged by size:
1933 754 564 117 13
BanII G_rGCy'C
Cuts at: 0 64 307 836 1129 3178 3381
Size: 64 243 529 293 2049 203
Fragments arranged by size:
2049 529 293 243 203 64
BbeI G_GCGC'C
Cuts at: 0 2067 3381
Size: 2067 1314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3144 3178 3381
Size: 3144 34 203
Fragments arranged by size:
3144 203 34
BclI T'GATC_A
Cuts at: 0 2676 3122 3381
Size: 2676 446 259
Fragments arranged by size:
2676 446 259
BfrBI ATG'CAT
Cuts at: 0 2792 3268 3381
Size: 2792 476 113
Fragments arranged by size:
2792 476 113
BlpI GC'TnA_GC
Cuts at: 0 749 3381
Size: 749 2632
Bme1580I G_kGCm'C
Cuts at: 0 428 1938 1949 2720 3381
Size: 428 1510 11 771 661
Fragments arranged by size:
1510 771 661 428 11
BmgBI CAC'GTC
Cuts at: 0 1240 2448 3381
Size: 1240 1208 933
Fragments arranged by size:
1240 1208 933
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 1683 2142 2347 3381
Size: 321 1362 459 205 1034
Fragments arranged by size:
1362 1034 459 321 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1113 1603 3054 3381
Size: 421 692 490 1451 327
Fragments arranged by size:
1451 692 490 421 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 3381
Size: 10 3371
BsaI GGTCTCn'nnnn_
Cuts at: 0 1735 2804 3381
Size: 1735 1069 577
Fragments arranged by size:
1735 1069 577
BsaAI yAC'GTr
Cuts at: 0 2634 3381
Size: 2634 747
BsaBI GATnn'nnATC
Cuts at: 0 511 3251 3381
Size: 511 2740 130
Fragments arranged by size:
2740 511 130
BsaHI Gr'CG_yC
Cuts at: 0 1880 2064 2851 3381
Size: 1880 184 787 530
Fragments arranged by size:
1880 787 530 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 753 783 1010 1040 1690 1720 2854 2884
Size: 753 30 227 30 650 30 1134 30
Cuts at: 2884 3135 3165 3381
Size: 251 30 216
Fragments arranged by size:
1134 753 650 251 227 216 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 129 826 3012 3381
Size: 129 697 2186 369
Fragments arranged by size:
2186 697 369 129
BseYI C'CCAG_C
Cuts at: 0 1599 1657 1939 3381
Size: 1599 58 282 1442
Fragments arranged by size:
1599 1442 282 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1918 2614 2894 3381
Size: 1918 696 280 487
Fragments arranged by size:
1918 696 487 280
BsiHKAI G_wGCw'C
Cuts at: 0 307 428 573 836 1129 1445 2273 3178
Size: 307 121 145 263 293 316 828 905
Cuts at: 3178 3381
Size: 203
Fragments arranged by size:
905 828 316 307 293 263 203 145
121
BsiWI C'GTAC_G
Cuts at: 0 1973 3381
Size: 1973 1408
BsmI GAATG_Cn'
Cuts at: 0 816 967 2792 3381
Size: 816 151 1825 589
Fragments arranged by size:
1825 816 589 151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 608 2669 3381
Size: 608 2061 712
Fragments arranged by size:
2061 712 608
Bsp1286I G_dGCh'C
Cuts at: 0 64 307 428 573 836 1129 1445 1938
Size: 64 243 121 145 263 293 316 493
Cuts at: 1938 1949 2273 2720 3178 3381
Size: 11 324 447 458 203
Fragments arranged by size:
493 458 447 324 316 293 263 243
203 145 121 64 11
BspHI T'CATG_A
Cuts at: 0 1064 3381
Size: 1064 2317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 837 1321 1753 2282 3381
Size: 837 484 432 529 1099
Fragments arranged by size:
1099 837 529 484 432
BsrDI GCAATG_nn'
Cuts at: 0 657 750 2916 3381
Size: 657 93 2166 465
Fragments arranged by size:
2166 657 465 93
BssSI C'ACGA_G
Cuts at: 0 2075 3381
Size: 2075 1306
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3010 3381
Size: 3010 371
BstBI TT'CG_AA
Cuts at: 0 1194 3381
Size: 1194 2187
BstEII G'GTnAC_C
Cuts at: 0 315 1920 2943 3381
Size: 315 1605 1023 438
Fragments arranged by size:
1605 1023 438 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 909 2096 3381
Size: 909 1187 1285
Fragments arranged by size:
1285 1187 909
BstYI r'GATC_y
Cuts at: 0 912 1058 1572 2826 3381
Size: 912 146 514 1254 555
Fragments arranged by size:
1254 912 555 514 146
Bsu36I CC'TnA_GG
Cuts at: 0 1540 3381
Size: 1540 1841
BtgI C'CryG_G
Cuts at: 0 159 860 2003 2355 2427 3381
Size: 159 701 1143 352 72 954
Fragments arranged by size:
1143 954 701 352 159 72
BtsI GCAGTG_nn'
Cuts at: 0 272 1360 2344 2438 2880 3381
Size: 272 1088 984 94 442 501
Fragments arranged by size:
1088 984 501 442 272 94
DraI TTT'AAA
Cuts at: 0 602 636 3285 3381
Size: 602 34 2649 96
Fragments arranged by size:
2649 602 96 34
DraIII CAC_nnn'GTG
Cuts at: 0 1365 2634 3315 3381
Size: 1365 1269 681 66
Fragments arranged by size:
1365 1269 681 66
DrdI GACnn_nn'nnGTC
Cuts at: 0 847 2251 2903 3381
Size: 847 1404 652 478
Fragments arranged by size:
1404 847 652 478
EaeI y'GGCC_r
Cuts at: 0 2223 2762 3381
Size: 2223 539 619
Fragments arranged by size:
2223 619 539
EarI CTCTTCn'nnn_
Cuts at: 0 183 1300 2672 2752 3381
Size: 183 1117 1372 80 629
Fragments arranged by size:
1372 1117 629 183 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2032 3381
Size: 2032 1349
EcoICRI GAG'CTC
Cuts at: 0 305 834 1127 3176 3381
Size: 305 529 293 2049 205
Fragments arranged by size:
2049 529 305 293 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 421 1113 1603 3054 3381
Size: 114 307 692 490 1451 327
Fragments arranged by size:
1451 692 490 327 307 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 117 3381
Size: 117 3264
EcoO109I rG'GnC_Cy
Cuts at: 0 1614 3381
Size: 1614 1767
EcoRI G'AATT_C
Cuts at: 0 656 941 2376 3381
Size: 656 285 1435 1005
Fragments arranged by size:
1435 1005 656 285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 736 741 3381
Size: 736 5 2640
Fragments arranged by size:
2640 736 5
HaeII r_GCGC'y
Cuts at: 0 1600 2025 2067 3381
Size: 1600 425 42 1314
Fragments arranged by size:
1600 1314 425 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1477 1509 1690 1722 3107 3139
Size: 1159 32 286 32 181 32 1385 32
Cuts at: 3139 3381
Size: 242
Fragments arranged by size:
1385 1159 286 242 181 32 32 32
32
HincII GTy'rAC
Cuts at: 0 852 3381
Size: 852 2529
HindIII A'AGCT_T
Cuts at: 0 718 981 3381
Size: 718 263 2400
Fragments arranged by size:
2400 718 263
KasI G'GCGC_C
Cuts at: 0 2063 3381
Size: 2063 1318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 2085 3381
Size: 970 1115 1296
Fragments arranged by size:
1296 1115 970
MslI CAynn'nnrTG
Cuts at: 0 1394 2545 3098 3381
Size: 1394 1151 553 283
Fragments arranged by size:
1394 1151 553 283
MspA1I CmG'CkG
Cuts at: 0 1420 1943 1981 1995 2005 2034 2357 3381
Size: 1420 523 38 14 10 29 323 1024
Fragments arranged by size:
1420 1024 523 323 38 29 14 10
NarI GG'CG_CC
Cuts at: 0 2064 3381
Size: 2064 1317
NdeI CA'TA_TG
Cuts at: 0 1391 3381
Size: 1391 1990
NsiI A_TGCA'T
Cuts at: 0 2794 3270 3381
Size: 2794 476 111
Fragments arranged by size:
2794 476 111
NspI r_CATG'y
Cuts at: 0 2536 3381
Size: 2536 845
PacI TTA_AT'TAA
Cuts at: 0 710 3381
Size: 710 2671
PasI CC'CwG_GG
Cuts at: 0 786 1518 1842 3381
Size: 786 732 324 1539
Fragments arranged by size:
1539 786 732 324
PfoI T'CCnGG_A
Cuts at: 0 396 1129 3381
Size: 396 733 2252
Fragments arranged by size:
2252 733 396
PmlI CAC'GTG
Cuts at: 0 2634 3381
Size: 2634 747
PpuMI rG'GwC_Cy
Cuts at: 0 1614 3381
Size: 1614 1767
PsiI TTA'TAA
Cuts at: 0 1013 3381
Size: 1013 2368
PstI C_TGCA'G
Cuts at: 0 2807 2887 3381
Size: 2807 80 494
Fragments arranged by size:
2807 494 80
PvuII CAG'CTG
Cuts at: 0 1420 1995 3381
Size: 1420 575 1386
Fragments arranged by size:
1420 1386 575
RsrII CG'GwC_CG
Cuts at: 0 2000 3381
Size: 2000 1381
SacI G_AGCT'C
Cuts at: 0 307 836 1129 3178 3381
Size: 307 529 293 2049 203
Fragments arranged by size:
2049 529 307 293 203
SacII CC_GC'GG
Cuts at: 0 2006 2358 3381
Size: 2006 352 1023
Fragments arranged by size:
2006 1023 352
SapI GCTCTTCn'nnn_
Cuts at: 0 2752 3381
Size: 2752 629
SfcI C'TryA_G
Cuts at: 0 429 2803 2883 3381
Size: 429 2374 80 498
Fragments arranged by size:
2374 498 429 80
SfoI GGC'GCC
Cuts at: 0 2065 3381
Size: 2065 1316
SmaI CCC'GGG
Cuts at: 0 167 3381
Size: 167 3214
SmlI C'TyrA_G
Cuts at: 0 25 1267 3381
Size: 25 1242 2114
Fragments arranged by size:
2114 1242 25
SpeI A'CTAG_T
Cuts at: 0 2239 3381
Size: 2239 1142
SphI G_CATG'C
Cuts at: 0 2536 3381
Size: 2536 845
StyI C'CwwG_G
Cuts at: 0 118 252 288 490 1315 3381
Size: 118 134 36 202 825 2066
Fragments arranged by size:
2066 825 202 134 118 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 303 980 2616 2786 3381
Size: 301 2 677 1636 170 595
Fragments arranged by size:
1636 677 595 301 170 2
TatI w'GTAC_w
Cuts at: 0 1584 1859 2164 3381
Size: 1584 275 305 1217
Fragments arranged by size:
1584 1217 305 275
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 753 785 802 834 1091 1123 1351 1383
Size: 753 32 17 32 257 32 228 32
Cuts at: 1383 2854 2886 3119 3135 3151 3167 3381
Size: 1471 32 233 16 16 16 214
Fragments arranged by size:
1471 753 257 233 228 214 32 32
32 32 32 17 16 16 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 295 1137 2096 2749 3381
Size: 295 842 959 653 632
Fragments arranged by size:
959 842 653 632 295
XmaI C'CCGG_G
Cuts at: 0 165 3381
Size: 165 3216
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AhdI AleI AlfI
AlwNI ApaLI ApoI AseI AvaI AvrII BamHI BanI
BanII BbeI BcgI BclI BfrBI BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I
NarI NdeI NsiI NspI PacI PasI PfoI PmlI
PpuMI PsiI PstI PvuII RsrII SacI SacII SapI
SfcI SfoI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AloI ApaI AscI
AsiSI BaeI BbsI BbvCI BciVI BglI BglII BmtI
BplI Bpu10I BsaWI BsiEI BspEI BsrBI BsrFI BsrGI
BssHII BstZ17I BtgZI ClaI CspCI EagI EcoRV FseI
FspI FspAI HpaI KpnI MfeI MluI MscI NaeI
NcoI NgoMIV NheI NotI NruI PciI PflMI PmeI
PpiI PshAI PspOMI PspXI PsrI PvuI SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI