Restriction map for
pF1KSDA1711
(Linear) (Six-base) MAPSORT of: KIAA1711.seq Check: 6201 from: 1 to: 3093
KIAA1711.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AatII G_ACGT'C
Cuts at: 0 1790 3093
Size: 1790 1303
AccI GT'mk_AC
Cuts at: 0 830 3093
Size: 830 2263
AclI AA'CG_TT
Cuts at: 0 2961 3093
Size: 2961 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 233 1307 1565 2636 3008 3093
Size: 1 232 1074 258 1071 372 85
Fragments arranged by size:
1074 1071 372 258 232 85 1
AflII C'TTAA_G
Cuts at: 0 2949 3093
Size: 2949 144
AflIII A'CryG_T
Cuts at: 0 550 940 1347 2367 3093
Size: 550 390 407 1020 726
Fragments arranged by size:
1020 726 550 407 390
AleI CACnn'nnGTG
Cuts at: 0 758 3093
Size: 758 2335
AlwNI CAG_nnn'CTG
Cuts at: 0 2305 2881 3093
Size: 2305 576 212
Fragments arranged by size:
2305 576 212
ApaLI G'TGCA_C
Cuts at: 0 2844 3093
Size: 2844 249
ApoI r'AATT_y
Cuts at: 0 93 301 1419 1453 2350 2499 2651 2786
Size: 93 208 1118 34 897 149 152 135
Cuts at: 2786 3093
Size: 307
Fragments arranged by size:
1118 897 307 208 152 149 135 93
34
AseI AT'TA_AT
Cuts at: 0 223 1802 3093
Size: 223 1579 1291
Fragments arranged by size:
1579 1291 223
AvaI C'yCGr_G
Cuts at: 0 353 3093
Size: 353 2740
AvrII C'CTAG_G
Cuts at: 0 1186 3093
Size: 1186 1907
BanI G'GyrC_C
Cuts at: 0 183 1917 3093
Size: 183 1734 1176
Fragments arranged by size:
1734 1176 183
BanII G_rGCy'C
Cuts at: 0 1362 3093
Size: 1362 1731
BbsI GAAGACnn'nnnn_
Cuts at: 0 1085 1331 1553 3093
Size: 1085 246 222 1540
Fragments arranged by size:
1540 1085 246 222
BbvCI CC'TCA_GC
Cuts at: 0 1820 3066 3093
Size: 1820 1246 27
Fragments arranged by size:
1820 1246 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 796 1633 3093
Size: 796 837 1460
Fragments arranged by size:
1460 837 796
BclI T'GATC_A
Cuts at: 0 903 2250 3093
Size: 903 1347 843
Fragments arranged by size:
1347 903 843
BfrBI ATG'CAT
Cuts at: 0 2095 2122 3093
Size: 2095 27 971
Fragments arranged by size:
2095 971 27
BglI GCCn_nnn'nGGC
Cuts at: 0 1742 3093
Size: 1742 1351
Bme1580I G_kGCm'C
Cuts at: 0 2848 2990 3093
Size: 2848 142 103
Fragments arranged by size:
2848 142 103
BmgBI CAC'GTC
Cuts at: 0 1329 3093
Size: 1329 1764
BmrI ACTGGGnnnn_n'
Cuts at: 0 1268 3093
Size: 1268 1825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2134 3093
Size: 2134 959
Bpu10I CC'TnA_GC
Cuts at: 0 1820 3066 3093
Size: 1820 1246 27
Fragments arranged by size:
1820 1246 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 3093
Size: 1375 1718
BsaAI yAC'GTr
Cuts at: 0 943 3093
Size: 943 2150
BsaBI GATnn'nnATC
Cuts at: 0 2459 3093
Size: 2459 634
BsaHI Gr'CG_yC
Cuts at: 0 1277 1787 3093
Size: 1277 510 1306
Fragments arranged by size:
1306 1277 510
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 908 938 2376 2406 3093
Size: 908 30 1438 30 687
Fragments arranged by size:
1438 908 687 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1162 1165 1183 1186 1737 2709 3093
Size: 1162 3 18 3 551 972 384
Fragments arranged by size:
1162 972 551 384 18 3 3
BseYI C'CCAG_C
Cuts at: 0 3080 3093
Size: 3080 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1521 3093
Size: 1521 1572
BsiHKAI G_wGCw'C
Cuts at: 0 964 1362 2848 3093
Size: 964 398 1486 245
Fragments arranged by size:
1486 964 398 245
BsmI GAATG_Cn'
Cuts at: 0 546 3007 3093
Size: 546 2461 86
Fragments arranged by size:
2461 546 86
Bsp1286I G_dGCh'C
Cuts at: 0 964 1362 2848 2990 3093
Size: 964 398 1486 142 103
Fragments arranged by size:
1486 964 398 142 103
BspHI T'CATG_A
Cuts at: 0 2675 3093
Size: 2675 418
BsrDI GCAATG_nn'
Cuts at: 0 383 3093
Size: 383 2710
BsrGI T'GTAC_A
Cuts at: 0 597 3093
Size: 597 2496
BssSI C'ACGA_G
Cuts at: 0 1670 3093
Size: 1670 1423
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1508 3093
Size: 1508 1585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 646 3093
Size: 646 2447
BstYI r'GATC_y
Cuts at: 0 396 2445 2900 3093
Size: 396 2049 455 193
Fragments arranged by size:
2049 455 396 193
BstZ17I GTA'TAC
Cuts at: 0 831 3093
Size: 831 2262
Bsu36I CC'TnA_GG
Cuts at: 0 2905 3093
Size: 2905 188
DraI TTT'AAA
Cuts at: 0 1601 2361 3015 3093
Size: 1601 760 654 78
Fragments arranged by size:
1601 760 654 78
DrdI GACnn_nn'nnGTC
Cuts at: 0 1786 3093
Size: 1786 1307
EaeI y'GGCC_r
Cuts at: 0 2 3093
Size: 2 3091
EarI CTCTTCn'nnn_
Cuts at: 0 173 503 1139 1160 1283 2526 2923 2973
Size: 173 330 636 21 123 1243 397 50
Cuts at: 2973 3027 3067 3093
Size: 54 40 26
Fragments arranged by size:
1243 636 397 330 173 123 54 50
40 26 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2010 3093
Size: 2010 1083
EcoICRI GAG'CTC
Cuts at: 0 1360 3093
Size: 1360 1733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 233 1307 1565 2134 2636 3008 3093
Size: 1 232 1074 258 569 502 372 85
Fragments arranged by size:
1074 569 502 372 258 232 85 1
EcoO109I rG'GnC_Cy
Cuts at: 0 643 3077 3093
Size: 643 2434 16
Fragments arranged by size:
2434 643 16
EcoRI G'AATT_C
Cuts at: 0 1453 3093
Size: 1453 1640
EcoRV GAT'ATC
Cuts at: 0 1965 3093
Size: 1965 1128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 52 3093
Size: 52 3041
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 787 819 888 920 1267 1299
Size: 388 32 367 32 69 32 347 32
Cuts at: 1299 1935 1967 2376 2408 2560 2592 3093
Size: 636 32 409 32 152 32 501
Fragments arranged by size:
636 501 409 388 367 347 152 69
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 442 801 896 2612 2819 3093
Size: 442 359 95 1716 207 274
Fragments arranged by size:
1716 442 359 274 207 95
HpaI GTT'AAC
Cuts at: 0 801 2612 3093
Size: 801 1811 481
Fragments arranged by size:
1811 801 481
MfeI C'AATT_G
Cuts at: 0 1922 3093
Size: 1922 1171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 852 1672 3093
Size: 852 820 1421
Fragments arranged by size:
1421 852 820
MscI TGG'CCA
Cuts at: 0 4 3093
Size: 4 3089
MslI CAynn'nnrTG
Cuts at: 0 593 758 3093
Size: 593 165 2335
Fragments arranged by size:
2335 593 165
MspA1I CmG'CkG
Cuts at: 0 664 2878 3093
Size: 664 2214 215
Fragments arranged by size:
2214 664 215
NsiI A_TGCA'T
Cuts at: 0 2097 2124 3093
Size: 2097 27 969
Fragments arranged by size:
2097 969 27
NspI r_CATG'y
Cuts at: 0 554 925 1351 2371 3093
Size: 554 371 426 1020 722
Fragments arranged by size:
1020 722 554 426 371
PciI A'CATG_T
Cuts at: 0 550 1347 2367 3093
Size: 550 797 1020 726
Fragments arranged by size:
1020 797 726 550
PfoI T'CCnGG_A
Cuts at: 0 1719 3093
Size: 1719 1374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1175 1207 3093
Size: 1175 32 1886
Fragments arranged by size:
1886 1175 32
PpuMI rG'GwC_Cy
Cuts at: 0 643 3077 3093
Size: 643 2434 16
Fragments arranged by size:
2434 643 16
PshAI GACnn'nnGTC
Cuts at: 0 216 3093
Size: 216 2877
PsiI TTA'TAA
Cuts at: 0 1634 2506 3093
Size: 1634 872 587
Fragments arranged by size:
1634 872 587
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2728 2760 3093
Size: 2728 32 333
Fragments arranged by size:
2728 333 32
PstI C_TGCA'G
Cuts at: 0 156 3093
Size: 156 2937
PvuII CAG'CTG
Cuts at: 0 664 2878 3093
Size: 664 2214 215
Fragments arranged by size:
2214 664 215
SacI G_AGCT'C
Cuts at: 0 1362 3093
Size: 1362 1731
SapI GCTCTTCn'nnn_
Cuts at: 0 503 3027 3093
Size: 503 2524 66
Fragments arranged by size:
2524 503 66
SfcI C'TryA_G
Cuts at: 0 152 3093
Size: 152 2941
SmlI C'TyrA_G
Cuts at: 0 1390 2949 3093
Size: 1390 1559 144
Fragments arranged by size:
1559 1390 144
SspI AAT'ATT
Cuts at: 0 227 2219 2238 3093
Size: 227 1992 19 855
Fragments arranged by size:
1992 855 227 19
StyI C'CwwG_G
Cuts at: 0 639 1186 3093
Size: 639 547 1907
Fragments arranged by size:
1907 639 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 1605 3093
Size: 261 1344 1488
Fragments arranged by size:
1488 1344 261
TatI w'GTAC_w
Cuts at: 0 597 1054 1107 2798 3093
Size: 597 457 53 1691 295
Fragments arranged by size:
1691 597 457 295 53
Tth111I GACn'n_nGTC
Cuts at: 0 1834 3093
Size: 1834 1259
XbaI T'CTAG_A
Cuts at: 0 2098 3093
Size: 2098 995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1945 2921 3093
Size: 1945 976 172
Fragments arranged by size:
1945 976 172
XmnI GAAnn'nnTTC
Cuts at: 0 1130 1679 1875 1933 3093
Size: 1130 549 196 58 1160
Fragments arranged by size:
1160 1130 549 196 58
ZraI GAC'GTC
Cuts at: 0 1788 3093
Size: 1788 1305
Enzymes that do cut:
AatII AccI AclI AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI AvaI AvrII BanI BanII BbsI
BbvCI BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I DraI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HpaI MfeI MmeI MscI MslI
MspA1I NsiI NspI PciI PfoI PpiI PpuMI PshAI
PsiI PsrI PstI PvuII SacI SapI SfcI SmlI
SspI StyI TaqII TatI Tth111I XbaI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AgeI AhdI AlfI AloI ApaI
AscI AsiSI BaeI BamHI BbeI BcgI BglII BlpI
BmtI BplI BsaI BsaWI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BssHII BstBI BstEII BtgI BtgZI
BtsI ClaI CspCI DraIII EagI EcoNI FseI FspI
FspAI HaeII HindIII KasI KpnI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI XhoI XmaI