Restriction map for
pF1KSDA1696
(Linear) (Six-base) MAPSORT of: KIAA1696.seq Check: 2499 from: 1 to: 1902
KIAA1696.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1065 1902
Size: 1065 837
AatII G_ACGT'C
Cuts at: 0 547 1902
Size: 547 1355
AccI GT'mk_AC
Cuts at: 0 1199 1763 1902
Size: 1199 564 139
Fragments arranged by size:
1199 564 139
AclI AA'CG_TT
Cuts at: 0 892 1902
Size: 892 1010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1492 1902
Size: 1492 410
AflIII A'CryG_T
Cuts at: 0 1375 1902
Size: 1375 527
AleI CACnn'nnGTG
Cuts at: 0 514 1902
Size: 514 1388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 344 532 1902
Size: 344 188 1370
Fragments arranged by size:
1370 344 188
AlwNI CAG_nnn'CTG
Cuts at: 0 57 424 577 1362 1470 1902
Size: 57 367 153 785 108 432
Fragments arranged by size:
785 432 367 153 108 57
ApoI r'AATT_y
Cuts at: 0 38 837 1902
Size: 38 799 1065
Fragments arranged by size:
1065 799 38
BamHI G'GATC_C
Cuts at: 0 78 1902
Size: 78 1824
BbsI GAAGACnn'nnnn_
Cuts at: 0 367 1902
Size: 367 1535
BbvCI CC'TCA_GC
Cuts at: 0 632 773 1902
Size: 632 141 1129
Fragments arranged by size:
1129 632 141
BfrBI ATG'CAT
Cuts at: 0 1652 1902
Size: 1652 250
BlpI GC'TnA_GC
Cuts at: 0 505 970 1630 1902
Size: 505 465 660 272
Fragments arranged by size:
660 505 465 272
BmgBI CAC'GTC
Cuts at: 0 682 1902
Size: 682 1220
BmrI ACTGGGnnnn_n'
Cuts at: 0 569 1902
Size: 569 1333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 917 1726 1902
Size: 523 394 809 176
Fragments arranged by size:
809 523 394 176
Bpu10I CC'TnA_GC
Cuts at: 0 632 773 1902
Size: 632 141 1129
Fragments arranged by size:
1129 632 141
BsaI GGTCTCn'nnnn_
Cuts at: 0 450 1902
Size: 450 1452
BsaHI Gr'CG_yC
Cuts at: 0 544 1902
Size: 544 1358
BsaWI w'CCGG_w
Cuts at: 0 204 1194 1902
Size: 204 990 708
Fragments arranged by size:
990 708 204
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 620 761 1096 1845 1902
Size: 620 141 335 749 57
Fragments arranged by size:
749 620 335 141 57
BseYI C'CCAG_C
Cuts at: 0 317 744 905 1902
Size: 317 427 161 997
Fragments arranged by size:
997 427 317 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1046 1259 1791 1851 1902
Size: 538 508 213 532 60 51
Fragments arranged by size:
538 532 508 213 60 51
BsiHKAI G_wGCw'C
Cuts at: 0 506 1902
Size: 506 1396
BsmI GAATG_Cn'
Cuts at: 0 93 1902
Size: 93 1809
Bsp1286I G_dGCh'C
Cuts at: 0 506 1902
Size: 506 1396
BspHI T'CATG_A
Cuts at: 0 111 1320 1902
Size: 111 1209 582
Fragments arranged by size:
1209 582 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 603 1065 1902
Size: 603 462 837
Fragments arranged by size:
837 603 462
BsrDI GCAATG_nn'
Cuts at: 0 226 464 1902
Size: 226 238 1438
Fragments arranged by size:
1438 238 226
BsrFI r'CCGG_y
Cuts at: 0 1839 1902
Size: 1839 63
BsrGI T'GTAC_A
Cuts at: 0 963 1902
Size: 963 939
BstAPI GCAn_nnn'nTGC
Cuts at: 0 424 1340 1902
Size: 424 916 562
Fragments arranged by size:
916 562 424
BstEII G'GTnAC_C
Cuts at: 0 474 1902
Size: 474 1428
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 717 1902
Size: 471 246 1185
Fragments arranged by size:
1185 471 246
BstYI r'GATC_y
Cuts at: 0 78 1442 1902
Size: 78 1364 460
Fragments arranged by size:
1364 460 78
BtgI C'CryG_G
Cuts at: 0 899 1492 1737 1902
Size: 899 593 245 165
Fragments arranged by size:
899 593 245 165
BtsI GCAGTG_nn'
Cuts at: 0 1247 1902
Size: 1247 655
DraI TTT'AAA
Cuts at: 0 1589 1902
Size: 1589 313
EaeI y'GGCC_r
Cuts at: 0 1303 1353 1902
Size: 1303 50 549
Fragments arranged by size:
1303 549 50
EarI CTCTTCn'nnn_
Cuts at: 0 1228 1276 1547 1886 1902
Size: 1228 48 271 339 16
Fragments arranged by size:
1228 339 271 48 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 917 1492 1726 1902
Size: 523 394 575 234 176
Fragments arranged by size:
575 523 394 234 176
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1708 1902
Size: 1708 194
EcoO109I rG'GnC_Cy
Cuts at: 0 1274 1902
Size: 1274 628
EcoRI G'AATT_C
Cuts at: 0 837 1902
Size: 837 1065
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 387 419 915 947 1902
Size: 387 32 496 32 955
Fragments arranged by size:
955 496 387 32 32
HincII GTy'rAC
Cuts at: 0 534 1902
Size: 534 1368
MfeI C'AATT_G
Cuts at: 0 1511 1902
Size: 1511 391
MscI TGG'CCA
Cuts at: 0 1305 1355 1902
Size: 1305 50 547
Fragments arranged by size:
1305 547 50
MslI CAynn'nnrTG
Cuts at: 0 469 514 1657 1902
Size: 469 45 1143 245
Fragments arranged by size:
1143 469 245 45
MspA1I CmG'CkG
Cuts at: 0 328 1722 1902
Size: 328 1394 180
Fragments arranged by size:
1394 328 180
NaeI GCC'GGC
Cuts at: 0 1841 1902
Size: 1841 61
NcoI C'CATG_G
Cuts at: 0 899 1492 1902
Size: 899 593 410
Fragments arranged by size:
899 593 410
NgoMIV G'CCGG_C
Cuts at: 0 1839 1902
Size: 1839 63
NsiI A_TGCA'T
Cuts at: 0 1654 1902
Size: 1654 248
NspI r_CATG'y
Cuts at: 0 1379 1440 1902
Size: 1379 61 462
Fragments arranged by size:
1379 462 61
PasI CC'CwG_GG
Cuts at: 0 1270 1902
Size: 1270 632
PciI A'CATG_T
Cuts at: 0 1375 1902
Size: 1375 527
PflMI CCAn_nnn'nTGG
Cuts at: 0 57 588 1498 1902
Size: 57 531 910 404
Fragments arranged by size:
910 531 404 57
PpuMI rG'GwC_Cy
Cuts at: 0 1274 1902
Size: 1274 628
PstI C_TGCA'G
Cuts at: 0 333 614 1902
Size: 333 281 1288
Fragments arranged by size:
1288 333 281
PvuII CAG'CTG
Cuts at: 0 328 1722 1902
Size: 328 1394 180
Fragments arranged by size:
1394 328 180
SanDI GG'GwC_CC
Cuts at: 0 1274 1902
Size: 1274 628
SfcI C'TryA_G
Cuts at: 0 16 274 329 371 610 1200 1760 1902
Size: 16 258 55 42 239 590 560 142
Fragments arranged by size:
590 560 258 239 142 55 42 16
StyI C'CwwG_G
Cuts at: 0 899 1430 1492 1902
Size: 899 531 62 410
Fragments arranged by size:
899 531 410 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1080 1902
Size: 1080 822
TatI w'GTAC_w
Cuts at: 0 963 1902
Size: 963 939
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 666 673 698 807 839 1902
Size: 641 25 7 25 109 32 1063
Fragments arranged by size:
1063 641 109 32 25 25 7
XbaI T'CTAG_A
Cuts at: 0 1140 1902
Size: 1140 762
XmnI GAAnn'nnTTC
Cuts at: 0 1487 1902
Size: 1487 415
ZraI GAC'GTC
Cuts at: 0 545 1902
Size: 545 1357
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AleI AlfI
AlwNI ApoI BamHI BbsI BbvCI BfrBI BlpI BmgBI
BmrI BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII
MfeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PpuMI PstI PvuII SanDI
SfcI StyI TaqII TatI TstI XbaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI AloI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BanI BanII
BbeI BcgI BciVI BclI BglI BglII Bme1580I BmtI
BplI BpuEI BsaAI BsaBI BsaXI BsiEI BsiWI BsmBI
BspEI BsrBI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MmeI NarI NdeI NheI NotI NruI
PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XcmI XhoI XmaI