Restriction map for
pF1KA1693
(Linear) (Six-base) MAPSORT of: KIAA1693.seq Check: 7614 from: 1 to: 2601
KIAA1693.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 456 725 1269 1520 2601
Size: 456 269 544 251 1081
Fragments arranged by size:
1081 544 456 269 251
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1415 1673 1907 1913 2132 2414 2601
Size: 602 813 258 234 6 219 282 187
Fragments arranged by size:
813 602 282 258 234 219 187 6
AflIII A'CryG_T
Cuts at: 0 2223 2601
Size: 2223 378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1882 2107 2601
Size: 1882 225 494
Fragments arranged by size:
1882 494 225
AlwNI CAG_nnn'CTG
Cuts at: 0 781 2601
Size: 781 1820
BanII G_rGCy'C
Cuts at: 0 275 1088 2601
Size: 275 813 1513
Fragments arranged by size:
1513 813 275
BbsI GAAGACnn'nnnn_
Cuts at: 0 704 1517 2060 2601
Size: 704 813 543 541
Fragments arranged by size:
813 704 543 541
BbvCI CC'TCA_GC
Cuts at: 0 211 266 446 1024 1079 1259 2601
Size: 211 55 180 578 55 180 1342
Fragments arranged by size:
1342 578 211 180 180 55 55
BfrBI ATG'CAT
Cuts at: 0 2209 2601
Size: 2209 392
BlpI GC'TnA_GC
Cuts at: 0 484 1297 1855 2080 2601
Size: 484 813 558 225 521
Fragments arranged by size:
813 558 521 484 225
BmgBI CAC'GTC
Cuts at: 0 2019 2244 2601
Size: 2019 225 357
Fragments arranged by size:
2019 357 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 769 1128 2601
Size: 315 454 359 1473
Fragments arranged by size:
1473 454 359 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 631 1177 1444 2116 2601
Size: 364 267 546 267 672 485
Fragments arranged by size:
672 546 485 364 267 267
Bpu10I CC'TnA_GC
Cuts at: 0 211 266 274 446 1024 1079 1087 1259
Size: 211 55 8 172 578 55 8 172
Cuts at: 1259 2601
Size: 1342
Fragments arranged by size:
1342 578 211 172 172 55 55 8
8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1054 2601
Size: 241 813 1547
Fragments arranged by size:
1547 813 241
BsaAI yAC'GTr
Cuts at: 0 2526 2601
Size: 2526 75
BsaWI w'CCGG_w
Cuts at: 0 396 1209 2601
Size: 396 813 1392
Fragments arranged by size:
1392 813 396
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1981 2011 2601
Size: 1981 30 590
Fragments arranged by size:
1981 590 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 283 379 544 1063 1096 1192 1672
Size: 250 33 96 165 519 33 96 480
Cuts at: 1672 2601
Size: 929
Fragments arranged by size:
929 519 480 250 165 96 96 33
33
BseYI C'CCAG_C
Cuts at: 0 77 890 2601
Size: 77 813 1711
Fragments arranged by size:
1711 813 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1378 2601
Size: 592 786 1223
Fragments arranged by size:
1223 786 592
BsiHKAI G_wGCw'C
Cuts at: 0 275 1088 2601
Size: 275 813 1513
Fragments arranged by size:
1513 813 275
Bsp1286I G_dGCh'C
Cuts at: 0 275 1088 2601
Size: 275 813 1513
Fragments arranged by size:
1513 813 275
BspEI T'CCGG_A
Cuts at: 0 396 1209 2601
Size: 396 813 1392
Fragments arranged by size:
1392 813 396
BspHI T'CATG_A
Cuts at: 0 741 2601
Size: 741 1860
BsrBI CCG'CTC
Cuts at: 0 362 1175 2601
Size: 362 813 1426
Fragments arranged by size:
1426 813 362
BsrFI r'CCGG_y
Cuts at: 0 13 826 2601
Size: 13 813 1775
Fragments arranged by size:
1775 813 13
BsrGI T'GTAC_A
Cuts at: 0 1569 2601
Size: 1569 1032
BstBI TT'CG_AA
Cuts at: 0 1447 2601
Size: 1447 1154
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2191 2601
Size: 2191 410
Bsu36I CC'TnA_GG
Cuts at: 0 599 1412 2601
Size: 599 813 1189
Fragments arranged by size:
1189 813 599
BtgI C'CryG_G
Cuts at: 0 642 1455 2601
Size: 642 813 1146
Fragments arranged by size:
1146 813 642
BtsI GCAGTG_nn'
Cuts at: 0 1977 2601
Size: 1977 624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 631 666 2601
Size: 631 35 1935
Fragments arranged by size:
1935 631 35
DraIII CAC_nnn'GTG
Cuts at: 0 2571 2601
Size: 2571 30
EaeI y'GGCC_r
Cuts at: 0 152 965 2601
Size: 152 813 1636
Fragments arranged by size:
1636 813 152
EarI CTCTTCn'nnn_
Cuts at: 0 176 798 989 1601 1649 2039 2381 2601
Size: 176 622 191 612 48 390 342 220
Fragments arranged by size:
622 612 390 342 220 191 176 48
EcoICRI GAG'CTC
Cuts at: 0 273 1086 2601
Size: 273 813 1515
Fragments arranged by size:
1515 813 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 602 631 1177 1415 1444 1673 1907
Size: 364 238 29 546 238 29 229 234
Cuts at: 1907 1913 2116 2132 2414 2601
Size: 6 203 16 282 187
Fragments arranged by size:
546 364 282 238 238 234 229 203
187 29 29 16 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1952 2601
Size: 1952 649
EcoO109I rG'GnC_Cy
Cuts at: 0 384 426 1197 1239 1846 1882 2601
Size: 384 42 771 42 607 36 719
Fragments arranged by size:
771 719 607 384 42 42 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 2601
Size: 718 32 1851
Fragments arranged by size:
1851 718 32
HincII GTy'rAC
Cuts at: 0 708 1521 1791 2601
Size: 708 813 270 810
Fragments arranged by size:
813 810 708 270
HindIII A'AGCT_T
Cuts at: 0 241 1054 2601
Size: 241 813 1547
Fragments arranged by size:
1547 813 241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1496 2601
Size: 683 813 1105
Fragments arranged by size:
1105 813 683
MscI TGG'CCA
Cuts at: 0 154 967 2601
Size: 154 813 1634
Fragments arranged by size:
1634 813 154
MspA1I CmG'CkG
Cuts at: 0 81 441 894 1254 2601
Size: 81 360 453 360 1347
Fragments arranged by size:
1347 453 360 360 81
NaeI GCC'GGC
Cuts at: 0 15 828 2601
Size: 15 813 1773
Fragments arranged by size:
1773 813 15
NcoI C'CATG_G
Cuts at: 0 1455 2601
Size: 1455 1146
NgoMIV G'CCGG_C
Cuts at: 0 13 826 2601
Size: 13 813 1775
Fragments arranged by size:
1775 813 13
NsiI A_TGCA'T
Cuts at: 0 2211 2601
Size: 2211 390
NspI r_CATG'y
Cuts at: 0 2227 2601
Size: 2227 374
PasI CC'CwG_GG
Cuts at: 0 417 563 1230 1376 2601
Size: 417 146 667 146 1225
Fragments arranged by size:
1225 667 417 146 146
PciI A'CATG_T
Cuts at: 0 2223 2601
Size: 2223 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 781 2601
Size: 781 1820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 1024 1056 2601
Size: 211 32 781 32 1545
Fragments arranged by size:
1545 781 211 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 426 1239 1846 2601
Size: 426 813 607 755
Fragments arranged by size:
813 755 607 426
PstI C_TGCA'G
Cuts at: 0 1646 2601
Size: 1646 955
PvuII CAG'CTG
Cuts at: 0 81 441 894 1254 2601
Size: 81 360 453 360 1347
Fragments arranged by size:
1347 453 360 360 81
SacI G_AGCT'C
Cuts at: 0 275 1088 2601
Size: 275 813 1513
Fragments arranged by size:
1513 813 275
SalI G'TCGA_C
Cuts at: 0 1519 2601
Size: 1519 1082
SapI GCTCTTCn'nnn_
Cuts at: 0 2381 2601
Size: 2381 220
ScaI AGT'ACT
Cuts at: 0 545 2601
Size: 545 2056
SexAI A'CCwGG_T
Cuts at: 0 2567 2601
Size: 2567 34
SfcI C'TryA_G
Cuts at: 0 1642 1965 2472 2601
Size: 1642 323 507 129
Fragments arranged by size:
1642 507 323 129
SmlI C'TyrA_G
Cuts at: 0 256 1069 2601
Size: 256 813 1532
Fragments arranged by size:
1532 813 256
StyI C'CwwG_G
Cuts at: 0 1455 2001 2058 2601
Size: 1455 546 57 543
Fragments arranged by size:
1455 546 543 57
TatI w'GTAC_w
Cuts at: 0 543 1569 2441 2601
Size: 543 1026 872 160
Fragments arranged by size:
1026 872 543 160
Tth111I GACn'n_nGTC
Cuts at: 0 703 1516 2601
Size: 703 813 1085
Fragments arranged by size:
1085 813 703
XbaI T'CTAG_A
Cuts at: 0 51 864 2601
Size: 51 813 1737
Fragments arranged by size:
1737 813 51
XmnI GAAnn'nnTTC
Cuts at: 0 518 1331 2601
Size: 518 813 1270
Fragments arranged by size:
1270 813 518
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI BanII BbsI BbvCI
BfrBI BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspHI BsrBI BsrFI BsrGI BstBI BstXI Bsu36I BtgI
BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I HincII HindIII MmeI MscI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PpiI
PpuMI PstI PvuII SacI SalI SapI ScaI SexAI
SfcI SmlI StyI TatI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BglI BglII Bme1580I BmtI BplI BsaI BsaBI
BsaHI BsiEI BsiWI BsmI BsmBI BspMI BsrDI BssHII
BssSI BstAPI BstEII BstYI BstZ17I BtgZI ClaI DraI
DrdI EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MslI
NarI NdeI NheI NotI NruI PacI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI XcmI XhoI XmaI ZraI