Restriction map for
pF1KSDA1691
(Linear) (Six-base) MAPSORT of: KIAA1691.seq Check: 5432 from: 1 to: 1569
KIAA1691.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 591 1569
Size: 591 978
AatII G_ACGT'C
Cuts at: 0 662 1569
Size: 662 907
AfeI AGC'GCT
Cuts at: 0 1127 1569
Size: 1127 442
AleI CACnn'nnGTG
Cuts at: 0 283 418 574 1060 1569
Size: 283 135 156 486 509
Fragments arranged by size:
509 486 283 156 135
AlwNI CAG_nnn'CTG
Cuts at: 0 126 459 510 519 930 1569
Size: 126 333 51 9 411 639
Fragments arranged by size:
639 411 333 126 51 9
AvaI C'yCGr_G
Cuts at: 0 100 484 1569
Size: 100 384 1085
Fragments arranged by size:
1085 384 100
BanI G'GyrC_C
Cuts at: 0 21 1569
Size: 21 1548
BanII G_rGCy'C
Cuts at: 0 446 491 799 1569
Size: 446 45 308 770
Fragments arranged by size:
770 446 308 45
BbeI G_GCGC'C
Cuts at: 0 25 1569
Size: 25 1544
BglI GCCn_nnn'nGGC
Cuts at: 0 151 1569
Size: 151 1418
BlpI GC'TnA_GC
Cuts at: 0 977 1569
Size: 977 592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 870 1559 1569
Size: 870 689 10
Fragments arranged by size:
870 689 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 544 604 783 1268 1491 1539 1569
Size: 481 63 60 179 485 223 48 30
Fragments arranged by size:
485 481 223 179 63 60 48 30
Bpu10I CC'TnA_GC
Cuts at: 0 67 1569
Size: 67 1502
BsaI GGTCTCn'nnnn_
Cuts at: 0 322 1360 1569
Size: 322 1038 209
Fragments arranged by size:
1038 322 209
BsaAI yAC'GTr
Cuts at: 0 831 1119 1569
Size: 831 288 450
Fragments arranged by size:
831 450 288
BsaHI Gr'CG_yC
Cuts at: 0 22 239 659 824 1569
Size: 22 217 420 165 745
Fragments arranged by size:
745 420 217 165 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 241 862 1018 1285 1288 1569
Size: 163 78 621 156 267 3 281
Fragments arranged by size:
621 281 267 163 156 78 3
BseYI C'CCAG_C
Cuts at: 0 71 445 534 630 732 758 768 1330
Size: 71 374 89 96 102 26 10 562
Cuts at: 1330 1569
Size: 239
Fragments arranged by size:
562 374 239 102 96 89 71 26
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 237 309 1569
Size: 28 209 72 1260
Fragments arranged by size:
1260 209 72 28
BsiEI CG_ry'CG
Cuts at: 0 548 1382 1569
Size: 548 834 187
Fragments arranged by size:
834 548 187
BsiHKAI G_wGCw'C
Cuts at: 0 233 1569
Size: 233 1336
BsmBI CGTCTCn'nnnn_
Cuts at: 0 520 1569
Size: 520 1049
Bsp1286I G_dGCh'C
Cuts at: 0 233 446 491 799 1569
Size: 233 213 45 308 770
Fragments arranged by size:
770 308 233 213 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 591 1076 1569
Size: 591 485 493
Fragments arranged by size:
591 493 485
BsrBI CCG'CTC
Cuts at: 0 465 1282 1569
Size: 465 817 287
Fragments arranged by size:
817 465 287
BsrFI r'CCGG_y
Cuts at: 0 47 209 295 1008 1132 1569
Size: 47 162 86 713 124 437
Fragments arranged by size:
713 437 162 124 86 47
BsrGI T'GTAC_A
Cuts at: 0 1337 1569
Size: 1337 232
BssHII G'CGCG_C
Cuts at: 0 764 1569
Size: 764 805
BssSI C'ACGA_G
Cuts at: 0 1533 1569
Size: 1533 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 51 126 519 1569
Size: 51 75 393 1050
Fragments arranged by size:
1050 393 75 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1569
Size: 843 726
BstYI r'GATC_y
Cuts at: 0 606 1569
Size: 606 963
BtgI C'CryG_G
Cuts at: 0 791 1291 1382 1569
Size: 791 500 91 187
Fragments arranged by size:
791 500 187 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 285 1569
Size: 258 27 1284
Fragments arranged by size:
1284 258 27
BtsI GCAGTG_nn'
Cuts at: 0 311 1368 1569
Size: 311 1057 201
Fragments arranged by size:
1057 311 201
EaeI y'GGCC_r
Cuts at: 0 2 386 473 545 1010 1379 1569
Size: 2 384 87 72 465 369 190
Fragments arranged by size:
465 384 369 190 87 72 2
EagI C'GGCC_G
Cuts at: 0 545 1379 1569
Size: 545 834 190
Fragments arranged by size:
834 545 190
EarI CTCTTCn'nnn_
Cuts at: 0 181 190 1183 1569
Size: 181 9 993 386
Fragments arranged by size:
993 386 181 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 355 454 610 1569
Size: 355 99 156 959
Fragments arranged by size:
959 355 156 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 544 604 783 1268 1491 1539 1569
Size: 481 63 60 179 485 223 48 30
Fragments arranged by size:
485 481 223 179 63 60 48 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 561 1569
Size: 561 1008
EcoO109I rG'GnC_Cy
Cuts at: 0 123 516 1020 1158 1252 1569
Size: 123 393 504 138 94 317
Fragments arranged by size:
504 393 317 138 123 94
HaeII r_GCGC'y
Cuts at: 0 25 296 1129 1569
Size: 25 271 833 440
Fragments arranged by size:
833 440 271 25
KasI G'GCGC_C
Cuts at: 0 21 1569
Size: 21 1548
MslI CAynn'nnrTG
Cuts at: 0 283 304 418 574 715 1060 1569
Size: 283 21 114 156 141 345 509
Fragments arranged by size:
509 345 283 156 141 114 21
MspA1I CmG'CkG
Cuts at: 0 471 758 1293 1343 1384 1454 1559 1569
Size: 471 287 535 50 41 70 105 10
Fragments arranged by size:
535 471 287 105 70 50 41 10
NaeI GCC'GGC
Cuts at: 0 211 297 1010 1569
Size: 211 86 713 559
Fragments arranged by size:
713 559 211 86
NarI GG'CG_CC
Cuts at: 0 22 1569
Size: 22 1547
NgoMIV G'CCGG_C
Cuts at: 0 209 295 1008 1569
Size: 209 86 713 561
Fragments arranged by size:
713 561 209 86
NspI r_CATG'y
Cuts at: 0 1329 1569
Size: 1329 240
PasI CC'CwG_GG
Cuts at: 0 998 1569
Size: 998 571
PflMI CCAn_nnn'nTGG
Cuts at: 0 1474 1569
Size: 1474 95
PfoI T'CCnGG_A
Cuts at: 0 395 1037 1569
Size: 395 642 532
Fragments arranged by size:
642 532 395
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1569
Size: 1020 549
PstI C_TGCA'G
Cuts at: 0 455 515 881 1073 1222 1569
Size: 455 60 366 192 149 347
Fragments arranged by size:
455 366 347 192 149 60
PvuII CAG'CTG
Cuts at: 0 471 758 1343 1569
Size: 471 287 585 226
Fragments arranged by size:
585 471 287 226
SacII CC_GC'GG
Cuts at: 0 1294 1385 1569
Size: 1294 91 184
Fragments arranged by size:
1294 184 91
SapI GCTCTTCn'nnn_
Cuts at: 0 190 1569
Size: 190 1379
ScaI AGT'ACT
Cuts at: 0 853 883 1569
Size: 853 30 686
Fragments arranged by size:
853 686 30
SfcI C'TryA_G
Cuts at: 0 451 511 877 1069 1218 1569
Size: 451 60 366 192 149 351
Fragments arranged by size:
451 366 351 192 149 60
SfoI GGC'GCC
Cuts at: 0 23 1569
Size: 23 1546
StyI C'CwwG_G
Cuts at: 0 704 1569
Size: 704 865
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 1569
Size: 21 1548
TatI w'GTAC_w
Cuts at: 0 851 881 1337 1418 1569
Size: 851 30 456 81 151
Fragments arranged by size:
851 456 151 81 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 1569
Size: 391 32 1146
Fragments arranged by size:
1146 391 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 711 1569
Size: 381 330 858
Fragments arranged by size:
858 381 330
ZraI GAC'GTC
Cuts at: 0 660 1569
Size: 660 909
Enzymes that do cut:
AarI AatII AfeI AleI AlwNI AvaI BanI BanII
BbeI BglI BlpI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I HaeII KasI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PflMI PfoI PpuMI PstI
PvuII SacII SapI ScaI SfcI SfoI StyI TaqII
TatI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmrI BmtI BpuEI BsaBI
BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrDI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI