Restriction map for
pF1KA1664
(Linear) (Six-base) MAPSORT of: KIAA1664.seq Check: 3715 from: 1 to: 2748
KIAA1664.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1694 1857 2748
Size: 1694 163 891
Fragments arranged by size:
1694 891 163
AatII G_ACGT'C
Cuts at: 0 262 2748
Size: 262 2486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 937 952 1069 1448 2164 2748
Size: 607 330 15 117 379 716 584
Fragments arranged by size:
716 607 584 379 330 117 15
AfeI AGC'GCT
Cuts at: 0 120 994 2748
Size: 120 874 1754
Fragments arranged by size:
1754 874 120
AflIII A'CryG_T
Cuts at: 0 665 1901 2030 2390 2748
Size: 665 1236 129 360 358
Fragments arranged by size:
1236 665 360 358 129
AgeI A'CCGG_T
Cuts at: 0 2132 2748
Size: 2132 616
AleI CACnn'nnGTG
Cuts at: 0 1771 2748
Size: 1771 977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 643 1722 2748
Size: 643 1079 1026
Fragments arranged by size:
1079 1026 643
AlwNI CAG_nnn'CTG
Cuts at: 0 280 1659 2337 2481 2529 2547 2591 2748
Size: 280 1379 678 144 48 18 44 157
Fragments arranged by size:
1379 678 280 157 144 48 44 18
ApaI G_GGCC'C
Cuts at: 0 353 2748
Size: 353 2395
ApaLI G'TGCA_C
Cuts at: 0 1189 1702 1774 2748
Size: 1189 513 72 974
Fragments arranged by size:
1189 974 513 72
ApoI r'AATT_y
Cuts at: 0 2005 2748
Size: 2005 743
AvaI C'yCGr_G
Cuts at: 0 383 415 1237 2748
Size: 383 32 822 1511
Fragments arranged by size:
1511 822 383 32
BamHI G'GATC_C
Cuts at: 0 1610 2655 2748
Size: 1610 1045 93
Fragments arranged by size:
1610 1045 93
BanI G'GyrC_C
Cuts at: 0 105 979 1486 1990 2135 2555 2748
Size: 105 874 507 504 145 420 193
Fragments arranged by size:
874 507 504 420 193 145 105
BanII G_rGCy'C
Cuts at: 0 59 149 353 1465 1604 1640 2748
Size: 59 90 204 1112 139 36 1108
Fragments arranged by size:
1112 1108 204 139 90 59 36
BbeI G_GCGC'C
Cuts at: 0 109 983 2559 2748
Size: 109 874 1576 189
Fragments arranged by size:
1576 874 189 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 481 1376 1418 2326 2748
Size: 481 895 42 908 422
Fragments arranged by size:
908 895 481 422 42
BbvCI CC'TCA_GC
Cuts at: 0 946 1887 2748
Size: 946 941 861
Fragments arranged by size:
946 941 861
BclI T'GATC_A
Cuts at: 0 1100 1394 2748
Size: 1100 294 1354
Fragments arranged by size:
1354 1100 294
BfrBI ATG'CAT
Cuts at: 0 2280 2748
Size: 2280 468
BglI GCCn_nnn'nGGC
Cuts at: 0 29 529 2748
Size: 29 500 2219
Fragments arranged by size:
2219 500 29
BlpI GC'TnA_GC
Cuts at: 0 1464 2748
Size: 1464 1284
Bme1580I G_kGCm'C
Cuts at: 0 173 353 525 1164 1193 1260 1502 1706
Size: 173 180 172 639 29 67 242 204
Cuts at: 1706 1778 2737 2748
Size: 72 959 11
Fragments arranged by size:
959 639 242 204 180 173 172 72
67 29 11
BmgBI CAC'GTC
Cuts at: 0 128 2748
Size: 128 2620
BmrI ACTGGGnnnn_n'
Cuts at: 0 2709 2748
Size: 2709 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 396 2748
Size: 348 48 2352
Fragments arranged by size:
2352 348 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 826 973 1479 1590 1636 1693 1932
Size: 501 325 147 506 111 46 57 239
Cuts at: 1932 1941 2551 2587 2748
Size: 9 610 36 161
Fragments arranged by size:
610 506 501 325 239 161 147 111
57 46 36 9
Bpu10I CC'TnA_GC
Cuts at: 0 333 946 1887 2748
Size: 333 613 941 861
Fragments arranged by size:
941 861 613 333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 745 1003 2748
Size: 553 192 258 1745
Fragments arranged by size:
1745 553 258 192
BsaI GGTCTCn'nnnn_
Cuts at: 0 1807 2748
Size: 1807 941
BsaAI yAC'GTr
Cuts at: 0 668 953 1902 2031 2232 2748
Size: 668 285 949 129 201 516
Fragments arranged by size:
949 668 516 285 201 129
BsaBI GATnn'nnATC
Cuts at: 0 1942 2748
Size: 1942 806
BsaHI Gr'CG_yC
Cuts at: 0 106 259 980 2556 2748
Size: 106 153 721 1576 192
Fragments arranged by size:
1576 721 192 153 106
BsaWI w'CCGG_w
Cuts at: 0 177 2132 2748
Size: 177 1955 616
Fragments arranged by size:
1955 616 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1714 1744 1891 1921 2377 2407 2748
Size: 1714 30 147 30 456 30 341
Fragments arranged by size:
1714 456 341 147 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 470 2449 2548 2748
Size: 470 1979 99 200
Fragments arranged by size:
1979 470 200 99
BseYI C'CCAG_C
Cuts at: 0 155 161 280 346 615 885 1806 2290
Size: 155 6 119 66 269 270 921 484
Cuts at: 2290 2299 2748
Size: 9 449
Fragments arranged by size:
921 484 449 270 269 155 119 66
9 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1692 2748
Size: 889 803 1056
Fragments arranged by size:
1056 889 803
BsiEI CG_ry'CG
Cuts at: 0 45 316 1270 1596 2748
Size: 45 271 954 326 1152
Fragments arranged by size:
1152 954 326 271 45
BsiHKAI G_wGCw'C
Cuts at: 0 1193 1465 1604 1706 1778 2047 2269 2432
Size: 1193 272 139 102 72 269 222 163
Cuts at: 2432 2748
Size: 316
Fragments arranged by size:
1193 316 272 269 222 163 139 102
72
BsmI GAATG_Cn'
Cuts at: 0 1535 2748
Size: 1535 1213
BsmBI CGTCTCn'nnnn_
Cuts at: 0 247 2748
Size: 247 2501
Bsp1286I G_dGCh'C
Cuts at: 0 59 149 173 353 525 1164 1193 1260
Size: 59 90 24 180 172 639 29 67
Cuts at: 1260 1465 1502 1604 1640 1706 1778 2047 2269
Size: 205 37 102 36 66 72 269 222
Cuts at: 2269 2432 2737 2748
Size: 163 305 11
Fragments arranged by size:
639 305 269 222 205 180 172 163
102 90 72 67 66 59 37 36
29 24 11
BspHI T'CATG_A
Cuts at: 0 1577 2748
Size: 1577 1171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1694 1857 2748
Size: 1694 163 891
Fragments arranged by size:
1694 891 163
BsrBI CCG'CTC
Cuts at: 0 47 183 378 1065 1242 2748
Size: 47 136 195 687 177 1506
Fragments arranged by size:
1506 687 195 177 136 47
BsrDI GCAATG_nn'
Cuts at: 0 627 963 2748
Size: 627 336 1785
Fragments arranged by size:
1785 627 336
BsrFI r'CCGG_y
Cuts at: 0 782 1265 1269 1620 2132 2558 2748
Size: 782 483 4 351 512 426 190
Fragments arranged by size:
782 512 483 426 351 190 4
BsrGI T'GTAC_A
Cuts at: 0 2684 2748
Size: 2684 64
BssSI C'ACGA_G
Cuts at: 0 1495 2244 2431 2748
Size: 1495 749 187 317
Fragments arranged by size:
1495 749 317 187
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1188 2591 2748
Size: 1188 1403 157
Fragments arranged by size:
1403 1188 157
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1719 2017 2748
Size: 1719 298 731
Fragments arranged by size:
1719 731 298
BstYI r'GATC_y
Cuts at: 0 1022 1610 2655 2748
Size: 1022 588 1045 93
Fragments arranged by size:
1045 1022 588 93
BtgI C'CryG_G
Cuts at: 0 51 672 1364 1451 2748
Size: 51 621 692 87 1297
Fragments arranged by size:
1297 692 621 87 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 951 1767 2748
Size: 951 816 981
Fragments arranged by size:
981 951 816
BtsI GCAGTG_nn'
Cuts at: 0 233 796 1162 1704 1706 2748
Size: 233 563 366 542 2 1042
Fragments arranged by size:
1042 563 542 366 233 2
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1433 1468 2748
Size: 1433 35 1280
Fragments arranged by size:
1433 1280 35
DraIII CAC_nnn'GTG
Cuts at: 0 1902 2031 2748
Size: 1902 129 717
Fragments arranged by size:
1902 717 129
EaeI y'GGCC_r
Cuts at: 0 42 313 812 1263 1267 1271 1593 1739
Size: 42 271 499 451 4 4 322 146
Cuts at: 1739 2748
Size: 1009
Fragments arranged by size:
1009 499 451 322 271 146 42 4
4
EagI C'GGCC_G
Cuts at: 0 42 313 1267 1593 2748
Size: 42 271 954 326 1155
Fragments arranged by size:
1155 954 326 271 42
EarI CTCTTCn'nnn_
Cuts at: 0 458 1081 2035 2600 2641 2748
Size: 458 623 954 565 41 107
Fragments arranged by size:
954 623 565 458 107 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2632 2748
Size: 2632 116
EcoICRI GAG'CTC
Cuts at: 0 1463 1602 2748
Size: 1463 139 1146
Fragments arranged by size:
1463 1146 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 607 826 937 952 973 1069 1448
Size: 501 106 219 111 15 21 96 379
Cuts at: 1448 1479 1590 1636 1693 1932 1941 2164 2551
Size: 31 111 46 57 239 9 223 387
Cuts at: 2551 2587 2748
Size: 36 161
Fragments arranged by size:
501 387 379 239 223 219 161 111
111 106 96 57 46 36 31 21
15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1930 2748
Size: 1930 818
EcoO109I rG'GnC_Cy
Cuts at: 0 350 1668 2748
Size: 350 1318 1080
Fragments arranged by size:
1318 1080 350
EcoRI G'AATT_C
Cuts at: 0 2005 2748
Size: 2005 743
FseI GG_CCGG'CC
Cuts at: 0 1269 1273 2748
Size: 1269 4 1475
Fragments arranged by size:
1475 1269 4
FspI TGC'GCA
Cuts at: 0 1895 2748
Size: 1895 853
HaeII r_GCGC'y
Cuts at: 0 109 122 603 983 996 2449 2547 2559
Size: 109 13 481 380 13 1453 98 12
Cuts at: 2559 2748
Size: 189
Fragments arranged by size:
1453 481 380 189 109 98 13 13
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 409 441 567 599 828 860
Size: 244 32 133 32 126 32 229 32
Cuts at: 860 1128 1160 1363 1395 1447 1474 1479 1506
Size: 268 32 203 32 52 27 5 27
Cuts at: 1506 1627 1659 2640 2672 2748
Size: 121 32 981 32 76
Fragments arranged by size:
981 268 244 229 203 133 126 121
76 52 32 32 32 32 32 32
32 32 27 27 5
KasI G'GCGC_C
Cuts at: 0 105 979 2555 2748
Size: 105 874 1576 193
Fragments arranged by size:
1576 874 193 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1126 2748
Size: 188 938 1622
Fragments arranged by size:
1622 938 188
MscI TGG'CCA
Cuts at: 0 814 2748
Size: 814 1934
MslI CAynn'nnrTG
Cuts at: 0 851 1390 1621 1771 2068 2748
Size: 851 539 231 150 297 680
Fragments arranged by size:
851 680 539 297 231 150
MspA1I CmG'CkG
Cuts at: 0 53 192 605 1158 1215 1656 1891 2142
Size: 53 139 413 553 57 441 235 251
Cuts at: 2142 2370 2478 2526 2591 2661 2748
Size: 228 108 48 65 70 87
Fragments arranged by size:
553 441 413 251 235 228 139 108
87 70 65 57 53 48
NaeI GCC'GGC
Cuts at: 0 1267 1271 2560 2748
Size: 1267 4 1289 188
Fragments arranged by size:
1289 1267 188 4
NarI GG'CG_CC
Cuts at: 0 106 980 2556 2748
Size: 106 874 1576 192
Fragments arranged by size:
1576 874 192 106
NcoI C'CATG_G
Cuts at: 0 1364 2748
Size: 1364 1384
NdeI CA'TA_TG
Cuts at: 0 2729 2748
Size: 2729 19
NgoMIV G'CCGG_C
Cuts at: 0 1265 1269 2558 2748
Size: 1265 4 1289 190
Fragments arranged by size:
1289 1265 190 4
NotI GC'GGCC_GC
Cuts at: 0 42 2748
Size: 42 2706
NsiI A_TGCA'T
Cuts at: 0 2282 2748
Size: 2282 466
NspI r_CATG'y
Cuts at: 0 2394 2748
Size: 2394 354
PciI A'CATG_T
Cuts at: 0 2390 2748
Size: 2390 358
PflMI CCAn_nnn'nTGG
Cuts at: 0 280 1689 2748
Size: 280 1409 1059
Fragments arranged by size:
1409 1059 280
PfoI T'CCnGG_A
Cuts at: 0 1606 2748
Size: 1606 1142
PmlI CAC'GTG
Cuts at: 0 668 953 2748
Size: 668 285 1795
Fragments arranged by size:
1795 668 285
PpuMI rG'GwC_Cy
Cuts at: 0 1668 2748
Size: 1668 1080
PshAI GACnn'nnGTC
Cuts at: 0 192 417 2748
Size: 192 225 2331
Fragments arranged by size:
2331 225 192
PspOMI G'GGCC_C
Cuts at: 0 349 2748
Size: 349 2399
PspXI vC'TCGA_Gb
Cuts at: 0 1237 2748
Size: 1237 1511
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2380 2412 2748
Size: 2380 32 336
Fragments arranged by size:
2380 336 32
PstI C_TGCA'G
Cuts at: 0 305 1114 1169 2596 2748
Size: 305 809 55 1427 152
Fragments arranged by size:
1427 809 305 152 55
PvuII CAG'CTG
Cuts at: 0 1215 1891 2142 2591 2748
Size: 1215 676 251 449 157
Fragments arranged by size:
1215 676 449 251 157
SacI G_AGCT'C
Cuts at: 0 1465 1604 2748
Size: 1465 139 1144
Fragments arranged by size:
1465 1144 139
SacII CC_GC'GG
Cuts at: 0 54 2748
Size: 54 2694
SapI GCTCTTCn'nnn_
Cuts at: 0 2035 2748
Size: 2035 713
SbfI CC_TGCA'GG
Cuts at: 0 1114 2748
Size: 1114 1634
ScaI AGT'ACT
Cuts at: 0 1354 2748
Size: 1354 1394
SfcI C'TryA_G
Cuts at: 0 301 639 1110 1165 1554 2157 2592 2748
Size: 301 338 471 55 389 603 435 156
Fragments arranged by size:
603 471 435 389 338 301 156 55
SfoI GGC'GCC
Cuts at: 0 107 981 2557 2748
Size: 107 874 1576 191
Fragments arranged by size:
1576 874 191 107
SgrAI Cr'CCGG_yG
Cuts at: 0 2558 2748
Size: 2558 190
SmaI CCC'GGG
Cuts at: 0 385 2748
Size: 385 2363
SmlI C'TyrA_G
Cuts at: 0 568 760 1018 1237 2748
Size: 568 192 258 219 1511
Fragments arranged by size:
1511 568 258 219 192
StuI AGG'CCT
Cuts at: 0 2572 2748
Size: 2572 176
StyI C'CwwG_G
Cuts at: 0 815 821 1364 1406 1502 2270 2708 2748
Size: 815 6 543 42 96 768 438 40
Fragments arranged by size:
815 768 543 438 96 42 40 6
TatI w'GTAC_w
Cuts at: 0 1352 1476 2393 2684 2748
Size: 1352 124 917 291 64
Fragments arranged by size:
1352 917 291 124 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1714 1746 2748
Size: 1714 32 1002
Fragments arranged by size:
1714 1002 32
Tth111I GACn'n_nGTC
Cuts at: 0 258 2337 2748
Size: 258 2079 411
Fragments arranged by size:
2079 411 258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 670 774 1212 1258 2748
Size: 525 145 104 438 46 1490
Fragments arranged by size:
1490 525 438 145 104 46
XhoI C'TCGA_G
Cuts at: 0 1237 2748
Size: 1237 1511
XmaI C'CCGG_G
Cuts at: 0 383 2748
Size: 383 2365
ZraI GAC'GTC
Cuts at: 0 260 2748
Size: 260 2488
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NsiI NspI PciI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SacII SapI SbfI ScaI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TatI TstI Tth111I XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AhdI AloI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BglII BmtI BsiWI
BspEI BssHII BstBI BstEII BstZ17I Bsu36I ClaI DraI
DrdI EcoRV FalI FspAI HincII HindIII HpaI KpnI
MfeI MluI NheI NruI PacI PasI PmeI PpiI
PsiI PvuI RsrII SalI SanDI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI
XmnI