Restriction map for
pF1KA1646
(Linear) (Six-base) MAPSORT of: KIAA1646.seq Check: 6016 from: 1 to: 1611
KIAA1646.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 291 1611
Size: 291 1320
AatII G_ACGT'C
Cuts at: 0 382 1611
Size: 382 1229
AccI GT'mk_AC
Cuts at: 0 650 1611
Size: 650 961
AfeI AGC'GCT
Cuts at: 0 58 1435 1611
Size: 58 1377 176
Fragments arranged by size:
1377 176 58
AflIII A'CryG_T
Cuts at: 0 1184 1611
Size: 1184 427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1541 1611
Size: 1541 70
AlwNI CAG_nnn'CTG
Cuts at: 0 354 1080 1493 1611
Size: 354 726 413 118
Fragments arranged by size:
726 413 354 118
ApaI G_GGCC'C
Cuts at: 0 119 1611
Size: 119 1492
ApoI r'AATT_y
Cuts at: 0 1369 1611
Size: 1369 242
AvaI C'yCGr_G
Cuts at: 0 111 665 1611
Size: 111 554 946
Fragments arranged by size:
946 554 111
BanI G'GyrC_C
Cuts at: 0 127 281 462 748 1611
Size: 127 154 181 286 863
Fragments arranged by size:
863 286 181 154 127
BanII G_rGCy'C
Cuts at: 0 80 90 112 119 1210 1611
Size: 80 10 22 7 1091 401
Fragments arranged by size:
1091 401 80 22 10 7
BbeI G_GCGC'C
Cuts at: 0 131 1611
Size: 131 1480
BbsI GAAGACnn'nnnn_
Cuts at: 0 1241 1611
Size: 1241 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 749 783 1611
Size: 749 34 828
Fragments arranged by size:
828 749 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1214 1611
Size: 1214 397
Bme1580I G_kGCm'C
Cuts at: 0 119 751 1445 1611
Size: 119 632 694 166
Fragments arranged by size:
694 632 166 119
BmgBI CAC'GTC
Cuts at: 0 1131 1611
Size: 1131 480
BmrI ACTGGGnnnn_n'
Cuts at: 0 677 1611
Size: 677 934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 388 1102 1263 1611
Size: 94 294 714 161 348
Fragments arranged by size:
714 348 294 161 94
Bpu10I CC'TnA_GC
Cuts at: 0 477 1611
Size: 477 1134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1399 1611
Size: 1399 212
BsaBI GATnn'nnATC
Cuts at: 0 700 1611
Size: 700 911
BsaHI Gr'CG_yC
Cuts at: 0 128 379 1611
Size: 128 251 1232
Fragments arranged by size:
1232 251 128
BsaWI w'CCGG_w
Cuts at: 0 692 1268 1611
Size: 692 576 343
Fragments arranged by size:
692 576 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 523 553 1611
Size: 316 30 177 30 1058
Fragments arranged by size:
1058 316 177 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 651 1099 1102 1150 1611
Size: 337 314 448 3 48 461
Fragments arranged by size:
461 448 337 314 48 3
BseYI C'CCAG_C
Cuts at: 0 861 1611
Size: 861 750
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 983 1458 1501 1611
Size: 595 388 475 43 110
Fragments arranged by size:
595 475 388 110 43
BsiHKAI G_wGCw'C
Cuts at: 0 276 1611
Size: 276 1335
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1235 1485 1611
Size: 1235 250 126
Fragments arranged by size:
1235 250 126
Bsp1286I G_dGCh'C
Cuts at: 0 80 90 112 119 276 751 1210 1445
Size: 80 10 22 7 157 475 459 235
Cuts at: 1445 1611
Size: 166
Fragments arranged by size:
475 459 235 166 157 80 22 10
7
BspEI T'CCGG_A
Cuts at: 0 692 1268 1611
Size: 692 576 343
Fragments arranged by size:
692 576 343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 291 1611
Size: 291 1320
BsrFI r'CCGG_y
Cuts at: 0 124 1611
Size: 124 1487
BssSI C'ACGA_G
Cuts at: 0 1571 1611
Size: 1571 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 354 1611
Size: 36 318 1257
Fragments arranged by size:
1257 318 36
BstYI r'GATC_y
Cuts at: 0 1015 1611
Size: 1015 596
BtgI C'CryG_G
Cuts at: 0 743 803 1611
Size: 743 60 808
Fragments arranged by size:
808 743 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 156 1611
Size: 156 1455
BtsI GCAGTG_nn'
Cuts at: 0 219 966 1545 1611
Size: 219 747 579 66
Fragments arranged by size:
747 579 219 66
DraI TTT'AAA
Cuts at: 0 947 1611
Size: 947 664
DraIII CAC_nnn'GTG
Cuts at: 0 1011 1611
Size: 1011 600
DrdI GACnn_nn'nnGTC
Cuts at: 0 571 1510 1611
Size: 571 939 101
Fragments arranged by size:
939 571 101
EaeI y'GGCC_r
Cuts at: 0 800 1166 1611
Size: 800 366 445
Fragments arranged by size:
800 445 366
EarI CTCTTCn'nnn_
Cuts at: 0 1577 1611
Size: 1577 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 120 599 1611
Size: 34 86 479 1012
Fragments arranged by size:
1012 479 86 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 388 1102 1263 1611
Size: 94 294 714 161 348
Fragments arranged by size:
714 348 294 161 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1417 1611
Size: 1417 194
EcoO109I rG'GnC_Cy
Cuts at: 0 115 1215 1512 1611
Size: 115 1100 297 99
Fragments arranged by size:
1100 297 115 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1452 1611
Size: 1452 159
HaeII r_GCGC'y
Cuts at: 0 60 131 644 775 1437 1611
Size: 60 71 513 131 662 174
Fragments arranged by size:
662 513 174 131 71 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 799 831 1289 1321 1611
Size: 133 32 634 32 458 32 290
Fragments arranged by size:
634 458 290 133 32 32 32
HincII GTy'rAC
Cuts at: 0 651 719 1611
Size: 651 68 892
Fragments arranged by size:
892 651 68
KasI G'GCGC_C
Cuts at: 0 127 1611
Size: 127 1484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 1611
Size: 1508 103
MscI TGG'CCA
Cuts at: 0 802 1168 1611
Size: 802 366 443
Fragments arranged by size:
802 443 366
MslI CAynn'nnrTG
Cuts at: 0 808 971 1183 1611
Size: 808 163 212 428
Fragments arranged by size:
808 428 212 163
MspA1I CmG'CkG
Cuts at: 0 27 287 330 1080 1554 1611
Size: 27 260 43 750 474 57
Fragments arranged by size:
750 474 260 57 43 27
NaeI GCC'GGC
Cuts at: 0 126 1611
Size: 126 1485
NarI GG'CG_CC
Cuts at: 0 128 1611
Size: 128 1483
NcoI C'CATG_G
Cuts at: 0 803 1611
Size: 803 808
NgoMIV G'CCGG_C
Cuts at: 0 124 1611
Size: 124 1487
NspI r_CATG'y
Cuts at: 0 517 1188 1611
Size: 517 671 423
Fragments arranged by size:
671 517 423
PasI CC'CwG_GG
Cuts at: 0 1211 1611
Size: 1211 400
PciI A'CATG_T
Cuts at: 0 1184 1611
Size: 1184 427
PfoI T'CCnGG_A
Cuts at: 0 1492 1611
Size: 1492 119
PpuMI rG'GwC_Cy
Cuts at: 0 1512 1611
Size: 1512 99
PspOMI G'GGCC_C
Cuts at: 0 115 1611
Size: 115 1496
PstI C_TGCA'G
Cuts at: 0 350 1611
Size: 350 1261
PvuII CAG'CTG
Cuts at: 0 330 1080 1554 1611
Size: 330 750 474 57
Fragments arranged by size:
750 474 330 57
SalI G'TCGA_C
Cuts at: 0 649 1611
Size: 649 962
SfcI C'TryA_G
Cuts at: 0 346 1611
Size: 346 1265
SfoI GGC'GCC
Cuts at: 0 129 1611
Size: 129 1482
SmaI CCC'GGG
Cuts at: 0 113 667 1611
Size: 113 554 944
Fragments arranged by size:
944 554 113
SmlI C'TyrA_G
Cuts at: 0 1414 1611
Size: 1414 197
StyI C'CwwG_G
Cuts at: 0 527 803 1021 1611
Size: 527 276 218 590
Fragments arranged by size:
590 527 276 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 1611
Size: 33 1578
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 715 747 1611
Size: 316 32 367 32 864
Fragments arranged by size:
864 367 316 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 984 1611
Size: 984 627
XmaI C'CCGG_G
Cuts at: 0 111 665 1611
Size: 111 554 946
Fragments arranged by size:
946 554 111
ZraI GAC'GTC
Cuts at: 0 380 1611
Size: 380 1231
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BcgI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrFI BssSI BstAPI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PpuMI PspOMI PstI PvuII SalI
SfcI SfoI SmaI SmlI StyI TaqII TstI Tth111I
XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BglII BlpI BmtI BplI BsaI
BsaAI BsiEI BsiWI BsmI BspHI BsrBI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI XbaI XcmI
XhoI XmnI